Archaeoglobus sulfaticallidus PM70-1

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Archaeoglobi; Archaeoglobales; Archaeoglobaceae; Archaeoglobus; Archaeoglobus sulfaticallidus

Average proteome isoelectric point is 6.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2213 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|N0BGQ3|N0BGQ3_9EURY Ribosomal RNA small subunit methyltransferase Nep1 OS=Archaeoglobus sulfaticallidus PM70-1 OX=387631 GN=nep1 PE=3 SV=1
MM1 pKa = 7.48VDD3 pKa = 3.34VKK5 pKa = 10.31KK6 pKa = 10.73KK7 pKa = 10.59CVGAVLMVAVLLMASVLPALAATTGQSSGSFEE39 pKa = 5.0LGNEE43 pKa = 4.03APQVVSVNLLLEE55 pKa = 4.86DD56 pKa = 4.51EE57 pKa = 4.55NTDD60 pKa = 3.97ANNQMTPQTEE70 pKa = 4.11YY71 pKa = 11.04ALKK74 pKa = 10.36IVASDD79 pKa = 3.35ANGYY83 pKa = 10.79GDD85 pKa = 3.24ISKK88 pKa = 8.37ITVYY92 pKa = 10.0IYY94 pKa = 11.23YY95 pKa = 10.15DD96 pKa = 3.65ANGDD100 pKa = 3.78GADD103 pKa = 4.41EE104 pKa = 4.93PGSDD108 pKa = 4.29NPQTQATYY116 pKa = 9.96QWTSSGGWTFVGPAGSTWGIDD137 pKa = 3.22TAADD141 pKa = 3.5QSRR144 pKa = 11.84GPVSTTTTTGTWYY157 pKa = 10.78LHH159 pKa = 6.67FIPGKK164 pKa = 10.27VATQSDD170 pKa = 4.09EE171 pKa = 5.72DD172 pKa = 4.03DD173 pKa = 3.89SKK175 pKa = 11.19WIIKK179 pKa = 9.48VVVEE183 pKa = 4.9DD184 pKa = 4.54GNSASDD190 pKa = 4.04SMKK193 pKa = 10.58DD194 pKa = 3.34TDD196 pKa = 6.36NEE198 pKa = 4.26MQWYY202 pKa = 10.47GEE204 pKa = 4.02IQVDD208 pKa = 4.17DD209 pKa = 4.34GSFDD213 pKa = 3.62FGSVNLGSSDD223 pKa = 4.41NPITTPNDD231 pKa = 3.25HH232 pKa = 7.05NIDD235 pKa = 3.2ITTVSNGQHH244 pKa = 6.79KK245 pKa = 10.05IQAKK249 pKa = 9.06TDD251 pKa = 3.75AQWSSGGNNVNVNTVSEE268 pKa = 4.46SPGAGEE274 pKa = 3.58ITIEE278 pKa = 4.06HH279 pKa = 6.54FNANDD284 pKa = 3.38EE285 pKa = 4.15TSAAIVQTSYY295 pKa = 11.55TDD297 pKa = 3.54VQGLTSIGQTSEE309 pKa = 3.72TGTTSEE315 pKa = 3.71IYY317 pKa = 8.66MWLTVGSEE325 pKa = 4.55GIMSGTYY332 pKa = 8.63TGTYY336 pKa = 8.69YY337 pKa = 10.96VQIANAA343 pKa = 3.92

Molecular weight: 36.35 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|N0BB60|N0BB60_9EURY Uncharacterized protein OS=Archaeoglobus sulfaticallidus PM70-1 OX=387631 GN=Asulf_00204 PE=4 SV=1
MM1 pKa = 7.34GKK3 pKa = 8.34KK4 pKa = 8.25TVGVKK9 pKa = 10.04FRR11 pKa = 11.84LAKK14 pKa = 10.29FYY16 pKa = 10.43RR17 pKa = 11.84QNRR20 pKa = 11.84RR21 pKa = 11.84APLWVTLKK29 pKa = 9.3TKK31 pKa = 10.51RR32 pKa = 11.84RR33 pKa = 11.84VFSSPKK39 pKa = 7.81RR40 pKa = 11.84RR41 pKa = 11.84HH42 pKa = 4.06WRR44 pKa = 11.84RR45 pKa = 11.84KK46 pKa = 7.81KK47 pKa = 10.93LKK49 pKa = 9.64VV50 pKa = 3.14

Molecular weight: 6.23 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2213

0

2213

604556

29

2362

273.2

30.86

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.627 ± 0.056

1.119 ± 0.025

5.437 ± 0.041

8.474 ± 0.066

4.583 ± 0.037

6.853 ± 0.047

1.516 ± 0.021

8.694 ± 0.047

7.714 ± 0.056

9.24 ± 0.063

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.61 ± 0.023

3.73 ± 0.042

3.617 ± 0.03

1.711 ± 0.02

5.407 ± 0.05

5.86 ± 0.038

4.261 ± 0.042

7.819 ± 0.046

0.975 ± 0.021

3.756 ± 0.04
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski