Cutibacterium granulosum DSM 20700
Average proteome isoelectric point is 6.16
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1962 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|U1F026|U1F026_9ACTN ABC transporter OS=Cutibacterium granulosum DSM 20700 OX=1160719 GN=H641_03400 PE=4 SV=1
MM1 pKa = 6.84 NTTYY5 pKa = 10.26 TITGMTCEE13 pKa = 4.4 HH14 pKa = 6.67 CVKK17 pKa = 10.72 AITEE21 pKa = 4.2 EE22 pKa = 4.2 VSAIDD27 pKa = 4.26 GVDD30 pKa = 3.0 KK31 pKa = 11.23 VYY33 pKa = 11.34 VSLEE37 pKa = 4.24 TGSMTIDD44 pKa = 3.26 SAEE47 pKa = 4.63 EE48 pKa = 3.57 ISMDD52 pKa = 5.19 AINKK56 pKa = 9.62 ALDD59 pKa = 3.37 EE60 pKa = 4.77 AGEE63 pKa = 4.16 YY64 pKa = 8.34 TATKK68 pKa = 10.43 ADD70 pKa = 3.77 SLDD73 pKa = 3.85 TAGHH77 pKa = 6.68 PEE79 pKa = 3.92 GG80 pKa = 4.71
Molecular weight: 8.45 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.809
IPC2_protein 4.037
IPC_protein 3.935
Toseland 3.757
ProMoST 4.088
Dawson 3.91
Bjellqvist 4.062
Wikipedia 3.821
Rodwell 3.783
Grimsley 3.681
Solomon 3.897
Lehninger 3.846
Nozaki 4.037
DTASelect 4.19
Thurlkill 3.808
EMBOSS 3.834
Sillero 4.062
Patrickios 1.901
IPC_peptide 3.897
IPC2_peptide 4.037
IPC2.peptide.svr19 3.939
Protein with the highest isoelectric point:
>tr|U1GJ04|U1GJ04_9ACTN DNA mismatch endonuclease Vsr OS=Cutibacterium granulosum DSM 20700 OX=1160719 GN=H641_05265 PE=3 SV=1
MM1 pKa = 7.4 GSVIKK6 pKa = 10.42 KK7 pKa = 8.47 RR8 pKa = 11.84 RR9 pKa = 11.84 KK10 pKa = 9.22 RR11 pKa = 11.84 MAKK14 pKa = 9.41 KK15 pKa = 9.99 KK16 pKa = 9.77 HH17 pKa = 5.81 RR18 pKa = 11.84 KK19 pKa = 8.55 LLKK22 pKa = 8.44 KK23 pKa = 9.1 TRR25 pKa = 11.84 IQRR28 pKa = 11.84 RR29 pKa = 11.84 RR30 pKa = 11.84 AGKK33 pKa = 9.79
Molecular weight: 4.07 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.493
IPC2_protein 11.14
IPC_protein 12.691
Toseland 12.866
ProMoST 13.349
Dawson 12.866
Bjellqvist 12.852
Wikipedia 13.334
Rodwell 12.735
Grimsley 12.91
Solomon 13.349
Lehninger 13.261
Nozaki 12.866
DTASelect 12.852
Thurlkill 12.866
EMBOSS 13.364
Sillero 12.866
Patrickios 12.457
IPC_peptide 13.349
IPC2_peptide 12.34
IPC2.peptide.svr19 9.071
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1962
0
1962
595837
22
1860
303.7
32.83
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.275 ± 0.06
0.995 ± 0.019
6.634 ± 0.056
5.338 ± 0.055
2.695 ± 0.038
8.52 ± 0.043
2.634 ± 0.026
4.599 ± 0.046
2.727 ± 0.05
9.304 ± 0.064
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.419 ± 0.024
2.354 ± 0.03
5.323 ± 0.046
3.465 ± 0.029
6.984 ± 0.052
6.161 ± 0.04
6.483 ± 0.051
8.705 ± 0.05
1.52 ± 0.026
1.866 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here