Mucilaginibacter lappiensis
Average proteome isoelectric point is 6.95
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5808 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1N7BPI7|A0A1N7BPI7_9SPHI Quinoprotein glucose dehydrogenase OS=Mucilaginibacter lappiensis OX=354630 GN=SAMN05421821_108102 PE=4 SV=1
MM1 pKa = 7.19 YY2 pKa = 7.77 WTLEE6 pKa = 4.21 LASHH10 pKa = 7.01 LEE12 pKa = 4.08 DD13 pKa = 4.93 APWPATKK20 pKa = 10.54 DD21 pKa = 3.44 EE22 pKa = 5.26 LIDD25 pKa = 3.48 YY26 pKa = 8.56 AIRR29 pKa = 11.84 SGAPVEE35 pKa = 4.53 VIEE38 pKa = 4.56 NLQALEE44 pKa = 4.69 DD45 pKa = 4.28 DD46 pKa = 4.31 GEE48 pKa = 4.31 PYY50 pKa = 11.05 EE51 pKa = 4.65 NIEE54 pKa = 4.81 EE55 pKa = 4.08 IWPDD59 pKa = 3.85 YY60 pKa = 7.47 PTKK63 pKa = 10.94 DD64 pKa = 3.05 DD65 pKa = 4.15 FFFNEE70 pKa = 4.51 DD71 pKa = 3.17 EE72 pKa = 4.46 YY73 pKa = 11.99
Molecular weight: 8.61 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.818
IPC2_protein 3.592
IPC_protein 3.528
Toseland 3.338
ProMoST 3.694
Dawson 3.516
Bjellqvist 3.681
Wikipedia 3.439
Rodwell 3.363
Grimsley 3.261
Solomon 3.478
Lehninger 3.439
Nozaki 3.643
DTASelect 3.795
Thurlkill 3.401
EMBOSS 3.452
Sillero 3.656
Patrickios 0.693
IPC_peptide 3.478
IPC2_peptide 3.617
IPC2.peptide.svr19 3.629
Protein with the highest isoelectric point:
>tr|A0A1N7FKT2|A0A1N7FKT2_9SPHI Glycosyl hydrolases family 38 C-terminal domain-containing protein OS=Mucilaginibacter lappiensis OX=354630 GN=SAMN05421821_11879 PE=4 SV=1
MM1 pKa = 7.58 GFGIIIIRR9 pKa = 11.84 ILFITCMVFIIGYY22 pKa = 8.73 VFGGFSKK29 pKa = 10.68 KK30 pKa = 10.08 SALTSITKK38 pKa = 9.69 VAAILVIILFVATNMLFMINRR59 pKa = 11.84 FEE61 pKa = 4.02 YY62 pKa = 10.7 FRR64 pKa = 11.84 GKK66 pKa = 8.3 GHH68 pKa = 7.28 RR69 pKa = 11.84 RR70 pKa = 11.84 QCDD73 pKa = 3.11 TVYY76 pKa = 11.08 SRR78 pKa = 11.84 GKK80 pKa = 8.04 HH81 pKa = 3.83 TT82 pKa = 4.27
Molecular weight: 9.34 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.327
IPC2_protein 9.882
IPC_protein 10.511
Toseland 10.628
ProMoST 10.292
Dawson 10.76
Bjellqvist 10.452
Wikipedia 10.935
Rodwell 11.125
Grimsley 10.818
Solomon 10.833
Lehninger 10.804
Nozaki 10.628
DTASelect 10.438
Thurlkill 10.643
EMBOSS 11.023
Sillero 10.687
Patrickios 10.906
IPC_peptide 10.833
IPC2_peptide 9.516
IPC2.peptide.svr19 8.348
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5808
0
5808
1973590
26
3993
339.8
38.06
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.505 ± 0.035
0.778 ± 0.01
5.433 ± 0.024
5.102 ± 0.035
4.893 ± 0.021
6.871 ± 0.032
1.99 ± 0.016
7.392 ± 0.03
6.928 ± 0.031
9.485 ± 0.035
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.219 ± 0.015
5.746 ± 0.031
3.935 ± 0.018
3.906 ± 0.019
3.726 ± 0.021
6.29 ± 0.025
6.001 ± 0.037
6.325 ± 0.024
1.232 ± 0.012
4.243 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here