Paracoccus seriniphilus
Average proteome isoelectric point is 6.25
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3981 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A239PMU0|A0A239PMU0_9RHOB Uncharacterized protein OS=Paracoccus seriniphilus OX=184748 GN=SAMN05444959_102128 PE=4 SV=1
MM1 pKa = 7.46 KK2 pKa = 10.38 LVIAAVTSSLIAGSAYY18 pKa = 10.31 AGGYY22 pKa = 4.76 TAPVVEE28 pKa = 5.3 PSPEE32 pKa = 5.01 PITVSPVVNDD42 pKa = 2.97 VSNWTGFYY50 pKa = 10.64 AGAQYY55 pKa = 11.04 GQGSADD61 pKa = 3.65 FNNGLGDD68 pKa = 3.81 SDD70 pKa = 3.74 ADD72 pKa = 3.55 AYY74 pKa = 10.49 GVHH77 pKa = 6.87 AGYY80 pKa = 10.82 NYY82 pKa = 10.92 DD83 pKa = 3.45 LGNWVVGGEE92 pKa = 3.98 LDD94 pKa = 3.99 YY95 pKa = 11.92 NKK97 pKa = 9.96 VDD99 pKa = 5.77 ADD101 pKa = 4.08 DD102 pKa = 5.95 LDD104 pKa = 5.07 DD105 pKa = 5.08 KK106 pKa = 11.99 GDD108 pKa = 3.69 MTRR111 pKa = 11.84 LRR113 pKa = 11.84 ARR115 pKa = 11.84 AGYY118 pKa = 10.98 DD119 pKa = 2.78 MGKK122 pKa = 10.16 FMPYY126 pKa = 8.09 VTLGAAHH133 pKa = 7.87 LSQDD137 pKa = 3.44 IPGGDD142 pKa = 3.15 ISEE145 pKa = 4.32 TDD147 pKa = 3.14 VTYY150 pKa = 10.68 GIGAEE155 pKa = 4.17 YY156 pKa = 10.52 KK157 pKa = 8.79 VTEE160 pKa = 4.2 QFTVGAEE167 pKa = 3.87 YY168 pKa = 9.89 TKK170 pKa = 10.73 QDD172 pKa = 3.51 FSDD175 pKa = 3.71 VADD178 pKa = 3.66 VDD180 pKa = 4.92 GLDD183 pKa = 4.57 LDD185 pKa = 3.88 TDD187 pKa = 4.29 MVQMRR192 pKa = 11.84 ASFRR196 pKa = 11.84 FF197 pKa = 3.41
Molecular weight: 20.99 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.69
IPC2_protein 3.821
IPC_protein 3.846
Toseland 3.605
ProMoST 4.024
Dawson 3.846
Bjellqvist 4.012
Wikipedia 3.821
Rodwell 3.668
Grimsley 3.516
Solomon 3.846
Lehninger 3.795
Nozaki 3.961
DTASelect 4.266
Thurlkill 3.668
EMBOSS 3.821
Sillero 3.973
Patrickios 1.138
IPC_peptide 3.834
IPC2_peptide 3.935
IPC2.peptide.svr19 3.847
Protein with the highest isoelectric point:
>tr|A0A239PLZ7|A0A239PLZ7_9RHOB Xaa-Pro aminopeptidase OS=Paracoccus seriniphilus OX=184748 GN=SAMN05444959_101368 PE=3 SV=1
MM1 pKa = 7.06 GQIINMILRR10 pKa = 11.84 QILRR14 pKa = 11.84 QAMQWGIRR22 pKa = 11.84 KK23 pKa = 8.73 GVNRR27 pKa = 11.84 MGQNPGKK34 pKa = 8.3 TGSRR38 pKa = 11.84 ASPQTARR45 pKa = 11.84 NQQQTVKK52 pKa = 10.58 RR53 pKa = 11.84 IRR55 pKa = 11.84 QAMRR59 pKa = 11.84 VIRR62 pKa = 11.84 RR63 pKa = 11.84 IGRR66 pKa = 3.42
Molecular weight: 7.64 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.272
IPC_protein 12.881
Toseland 13.042
ProMoST 13.539
Dawson 13.042
Bjellqvist 13.042
Wikipedia 13.525
Rodwell 12.647
Grimsley 13.086
Solomon 13.539
Lehninger 13.437
Nozaki 13.042
DTASelect 13.042
Thurlkill 13.042
EMBOSS 13.539
Sillero 13.042
Patrickios 12.369
IPC_peptide 13.539
IPC2_peptide 12.53
IPC2.peptide.svr19 9.218
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3981
0
3981
1275686
26
2132
320.4
34.85
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.13 ± 0.049
0.925 ± 0.014
6.05 ± 0.034
5.72 ± 0.033
3.624 ± 0.026
8.639 ± 0.037
2.122 ± 0.018
5.396 ± 0.025
2.866 ± 0.027
10.213 ± 0.046
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.91 ± 0.018
2.59 ± 0.018
5.165 ± 0.026
3.47 ± 0.022
7.076 ± 0.037
5.398 ± 0.025
5.168 ± 0.028
6.998 ± 0.032
1.419 ± 0.017
2.121 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here