Sanxia tombus-like virus 2

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 7.53

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KGQ8|A0A1L3KGQ8_9VIRU Capsid protein OS=Sanxia tombus-like virus 2 OX=1923386 PE=3 SV=1
MM1 pKa = 7.89AKK3 pKa = 9.66TNQNKK8 pKa = 9.18NKK10 pKa = 9.6PMKK13 pKa = 10.48QNGQVARR20 pKa = 11.84QSASKK25 pKa = 10.42GFTSMGYY32 pKa = 10.53SNNNQDD38 pKa = 3.46PRR40 pKa = 11.84FSNSSGGTVIKK51 pKa = 10.42HH52 pKa = 6.01RR53 pKa = 11.84EE54 pKa = 4.05LIEE57 pKa = 4.14LVSSQTQFNVAQKK70 pKa = 10.29RR71 pKa = 11.84INPADD76 pKa = 3.9RR77 pKa = 11.84ATFPWLSAVAAKK89 pKa = 10.05YY90 pKa = 9.34EE91 pKa = 4.07KK92 pKa = 10.78YY93 pKa = 10.12RR94 pKa = 11.84FRR96 pKa = 11.84KK97 pKa = 9.77LKK99 pKa = 10.28FQMVPHH105 pKa = 7.1APTTASGTLGMYY117 pKa = 9.87IDD119 pKa = 5.16YY120 pKa = 10.83DD121 pKa = 3.92PSDD124 pKa = 3.83VPAPNASAFFSSGNAVTSQIWLEE147 pKa = 4.02TSVQAKK153 pKa = 7.37PQQMQLFNQDD163 pKa = 3.99SIQMADD169 pKa = 5.19LEE171 pKa = 4.89IKK173 pKa = 9.23WFDD176 pKa = 3.31YY177 pKa = 11.21ARR179 pKa = 11.84IFFYY183 pKa = 9.97MQSPGSNGAHH193 pKa = 5.3TAWLFVDD200 pKa = 4.11YY201 pKa = 10.08EE202 pKa = 4.39IEE204 pKa = 4.63LFTPTANVEE213 pKa = 4.28GSKK216 pKa = 8.91FTPDD220 pKa = 3.37FTGFAYY226 pKa = 7.53WTTAQNTAAFTNAPTILRR244 pKa = 11.84GLEE247 pKa = 3.87YY248 pKa = 10.65VQEE251 pKa = 4.5DD252 pKa = 4.61GEE254 pKa = 4.5AKK256 pKa = 9.65LTVPYY261 pKa = 9.98SGYY264 pKa = 10.88YY265 pKa = 10.31RR266 pKa = 11.84ITTLTANTNSGASVTEE282 pKa = 4.02IFEE285 pKa = 4.63SPTCRR290 pKa = 11.84IINGLDD296 pKa = 3.5GEE298 pKa = 4.93GNPTTRR304 pKa = 11.84DD305 pKa = 3.07QTVIVQLQQGEE316 pKa = 4.97TYY318 pKa = 10.71VPLRR322 pKa = 11.84MTTGILDD329 pKa = 4.25LFADD333 pKa = 4.62PDD335 pKa = 4.05NDD337 pKa = 3.84PSPLGVEE344 pKa = 4.4VVLLQASS351 pKa = 3.68

Molecular weight: 38.9 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KGQ8|A0A1L3KGQ8_9VIRU Capsid protein OS=Sanxia tombus-like virus 2 OX=1923386 PE=3 SV=1
MM1 pKa = 7.26SRR3 pKa = 11.84VDD5 pKa = 3.41PGIPGGTPLQHH16 pKa = 6.48KK17 pKa = 9.57SSRR20 pKa = 11.84NLMLIIIDD28 pKa = 3.32GHH30 pKa = 7.58IPMVSLMGQSSVILLGAQSRR50 pKa = 11.84SFAFLFVGLTCSTLWKK66 pKa = 9.52KK67 pKa = 10.8VLMRR71 pKa = 11.84YY72 pKa = 9.48FSLMRR77 pKa = 11.84NMSRR81 pKa = 11.84CCSSASAGSKK91 pKa = 8.57QRR93 pKa = 11.84EE94 pKa = 4.27GNLLRR99 pKa = 11.84TCYY102 pKa = 10.18RR103 pKa = 11.84QIFWDD108 pKa = 3.93CLSTGRR114 pKa = 11.84DD115 pKa = 3.54QPSGLPYY122 pKa = 10.77VLDD125 pKa = 3.3WLAWLDD131 pKa = 3.64AGG133 pKa = 5.22

Molecular weight: 14.81 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

1008

133

524

336.0

37.94

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.448 ± 1.234

1.984 ± 0.889

4.365 ± 0.274

4.96 ± 1.101

5.258 ± 0.364

6.944 ± 0.518

1.786 ± 0.534

4.464 ± 0.214

4.663 ± 0.507

8.532 ± 1.423

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.075 ± 0.511

4.464 ± 0.958

5.06 ± 0.479

5.853 ± 0.531

6.349 ± 1.381

7.937 ± 1.129

5.754 ± 1.894

6.845 ± 0.964

1.786 ± 0.297

3.472 ± 0.335
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski