Magnaporthe oryzae virus 2
Average proteome isoelectric point is 6.96
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A9ZNC9|A9ZNC9_9VIRU RNA-directed RNA polymerase OS=Magnaporthe oryzae virus 2 OX=441999 GN=RdRp PE=3 SV=1
MM1 pKa = 7.87 ASTQGFPSIEE11 pKa = 3.93 ASQLASTLADD21 pKa = 3.32 PRR23 pKa = 11.84 GALLQTAEE31 pKa = 3.91 EE32 pKa = 4.21 YY33 pKa = 10.02 RR34 pKa = 11.84 RR35 pKa = 11.84 YY36 pKa = 9.77 RR37 pKa = 11.84 AGVFIGTMEE46 pKa = 4.23 HH47 pKa = 6.0 GQRR50 pKa = 11.84 GSYY53 pKa = 9.29 GRR55 pKa = 11.84 SIFYY59 pKa = 10.42 EE60 pKa = 3.84 VGFRR64 pKa = 11.84 HH65 pKa = 6.5 AGARR69 pKa = 11.84 DD70 pKa = 3.26 ALAPRR75 pKa = 11.84 SEE77 pKa = 4.04 HH78 pKa = 7.11 AIPIDD83 pKa = 3.64 CSLDD87 pKa = 3.35 INAVEE92 pKa = 3.96 AANFEE97 pKa = 4.45 GYY99 pKa = 10.34 ARR101 pKa = 11.84 RR102 pKa = 11.84 FSNFSPQWQKK112 pKa = 10.44 MDD114 pKa = 3.66 LAGMVEE120 pKa = 4.33 RR121 pKa = 11.84 LAKK124 pKa = 10.17 GVAAASLYY132 pKa = 11.14 GNMGTVNLIAGQPTRR147 pKa = 11.84 VTALGTLDD155 pKa = 3.78 SPQTASIDD163 pKa = 3.72 SVFIPRR169 pKa = 11.84 TVDD172 pKa = 3.11 TVGNDD177 pKa = 2.94 HH178 pKa = 6.29 VFAVLAAAANGEE190 pKa = 4.34 GAAVTTDD197 pKa = 3.57 VLRR200 pKa = 11.84 LDD202 pKa = 4.82 ANTNQPIIPSVVGPAFATACVEE224 pKa = 3.92 ALRR227 pKa = 11.84 ILGANFEE234 pKa = 4.56 SSGGGDD240 pKa = 3.22 VFAYY244 pKa = 10.43 ALTRR248 pKa = 11.84 GIHH251 pKa = 5.37 SCVSVVAHH259 pKa = 6.01 TDD261 pKa = 2.67 EE262 pKa = 4.92 GGFMRR267 pKa = 11.84 NLFRR271 pKa = 11.84 HH272 pKa = 5.34 AAFRR276 pKa = 11.84 TPYY279 pKa = 10.58 GGVNQSLRR287 pKa = 11.84 AFPSLPALAGTANTAVAAWVDD308 pKa = 4.58 AIALKK313 pKa = 9.18 TAAAVAHH320 pKa = 6.94 CDD322 pKa = 3.63 PLVPGPGGLFPTVFTSADD340 pKa = 3.7 GPISPAGTPEE350 pKa = 4.45 GEE352 pKa = 4.34 PTDD355 pKa = 3.72 ADD357 pKa = 3.1 ARR359 pKa = 11.84 AIGRR363 pKa = 11.84 QISADD368 pKa = 3.49 LGRR371 pKa = 11.84 FAPLYY376 pKa = 9.66 MRR378 pKa = 11.84 ALGQIVGTRR387 pKa = 11.84 TVSGVAEE394 pKa = 4.11 SHH396 pKa = 6.31 FCTVASLALDD406 pKa = 3.35 QGGGMVDD413 pKa = 2.54 RR414 pKa = 11.84 HH415 pKa = 5.87 LRR417 pKa = 11.84 HH418 pKa = 5.82 KK419 pKa = 9.35 TVAPYY424 pKa = 9.8 FWIEE428 pKa = 3.91 PTSLIPRR435 pKa = 11.84 DD436 pKa = 4.0 FLDD439 pKa = 4.39 SPAEE443 pKa = 3.92 HH444 pKa = 6.87 AGYY447 pKa = 10.4 ASKK450 pKa = 9.18 VTPGHH455 pKa = 5.86 VAHH458 pKa = 6.97 EE459 pKa = 4.08 PCFEE463 pKa = 3.97 KK464 pKa = 10.72 FALLEE469 pKa = 4.37 RR470 pKa = 11.84 GTTANHH476 pKa = 5.26 ATVAFKK482 pKa = 10.17 MRR484 pKa = 11.84 SARR487 pKa = 11.84 TSALICAYY495 pKa = 10.21 AGDD498 pKa = 4.24 PAVLADD504 pKa = 3.28 IKK506 pKa = 10.83 LYY508 pKa = 10.83 QFDD511 pKa = 3.87 EE512 pKa = 5.04 DD513 pKa = 4.55 SVVLAGDD520 pKa = 3.55 QGPTAGPVPVKK531 pKa = 10.27 HH532 pKa = 6.24 AAADD536 pKa = 4.57 PISSYY541 pKa = 9.56 LWRR544 pKa = 11.84 RR545 pKa = 11.84 GQSCFPAPAEE555 pKa = 4.37 FINTNSNYY563 pKa = 9.02 GAKK566 pKa = 10.18 VSLITWDD573 pKa = 3.37 NDD575 pKa = 3.19 FNATVPDD582 pKa = 4.14 LPHH585 pKa = 5.6 EE586 pKa = 4.42 HH587 pKa = 6.51 EE588 pKa = 4.6 MAHH591 pKa = 6.0 EE592 pKa = 4.78 SISFRR597 pKa = 11.84 VTRR600 pKa = 11.84 PTGLPTGPSNYY611 pKa = 8.89 EE612 pKa = 3.46 DD613 pKa = 3.64 RR614 pKa = 11.84 EE615 pKa = 4.05 ARR617 pKa = 11.84 RR618 pKa = 11.84 ARR620 pKa = 11.84 SRR622 pKa = 11.84 AAIALAQATVRR633 pKa = 11.84 ARR635 pKa = 11.84 AFGGAASPTMAVSNVPPSFGEE656 pKa = 3.64 SDD658 pKa = 3.12 TTYY661 pKa = 11.22 RR662 pKa = 11.84 DD663 pKa = 3.01 TDD665 pKa = 3.7 YY666 pKa = 11.61 SEE668 pKa = 4.77 YY669 pKa = 10.52 RR670 pKa = 11.84 ADD672 pKa = 4.07 PGTTTGRR679 pKa = 11.84 GIGGNVGGAGTSRR692 pKa = 11.84 GAPLPPTAHH701 pKa = 7.03 HH702 pKa = 6.51 EE703 pKa = 4.14 PQRR706 pKa = 11.84 APRR709 pKa = 11.84 LPPQGPLGGGPGGGAAPPPQGGAGVPPPAAAPPTGPNPPAGVLDD753 pKa = 4.0 EE754 pKa = 4.81 RR755 pKa = 11.84 QLDD758 pKa = 4.21 PLPAPHH764 pKa = 7.07 IPGAFDD770 pKa = 4.25 RR771 pKa = 11.84 EE772 pKa = 4.65 GNNNEE777 pKa = 4.04 QAAGAQPAPAQQ788 pKa = 3.57
Molecular weight: 82.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.712
IPC2_protein 5.601
IPC_protein 5.664
Toseland 6.122
ProMoST 6.008
Dawson 5.931
Bjellqvist 5.944
Wikipedia 5.944
Rodwell 5.919
Grimsley 6.3
Solomon 5.931
Lehninger 5.919
Nozaki 6.198
DTASelect 6.389
Thurlkill 6.402
EMBOSS 6.364
Sillero 6.3
Patrickios 4.139
IPC_peptide 5.944
IPC2_peptide 6.313
IPC2.peptide.svr19 6.205
Protein with the highest isoelectric point:
>tr|A9ZNC9|A9ZNC9_9VIRU RNA-directed RNA polymerase OS=Magnaporthe oryzae virus 2 OX=441999 GN=RdRp PE=3 SV=1
MM1 pKa = 7.7 IEE3 pKa = 4.5 LPVATRR9 pKa = 11.84 AKK11 pKa = 9.6 ATGSLGQYY19 pKa = 10.61 LKK21 pKa = 11.28 GLLDD25 pKa = 3.84 RR26 pKa = 11.84 RR27 pKa = 11.84 WLRR30 pKa = 11.84 RR31 pKa = 11.84 MAQLDD36 pKa = 3.64 YY37 pKa = 10.45 AGQMSEE43 pKa = 3.99 IYY45 pKa = 10.75 APFWNGTRR53 pKa = 11.84 VSPLVRR59 pKa = 11.84 AAGAFLVPAMPIQVPVPHH77 pKa = 6.68 NALTTLLQATLPPLDD92 pKa = 3.98 GLAPRR97 pKa = 11.84 QSRR100 pKa = 11.84 PSKK103 pKa = 10.74 DD104 pKa = 2.96 GWSLSASRR112 pKa = 11.84 TRR114 pKa = 11.84 DD115 pKa = 3.36 AFPLKK120 pKa = 10.66 SNPAASNKK128 pKa = 9.17 VNVYY132 pKa = 9.92 LNEE135 pKa = 4.04 VMGSLTGLSPALAIQAEE152 pKa = 4.55 ATLSTIAYY160 pKa = 9.76 SGVANDD166 pKa = 3.66 AAAAVIIYY174 pKa = 9.98 GYY176 pKa = 10.07 GLSHH180 pKa = 7.16 NGVCDD185 pKa = 3.96 PFDD188 pKa = 3.34 TAARR192 pKa = 11.84 LVRR195 pKa = 11.84 APEE198 pKa = 3.89 YY199 pKa = 10.64 CKK201 pKa = 10.87 ALTTFLKK208 pKa = 10.18 ATGGNSTRR216 pKa = 11.84 LGALLAEE223 pKa = 4.86 ANTLLGRR230 pKa = 11.84 DD231 pKa = 3.34 VGAIDD236 pKa = 4.11 LRR238 pKa = 11.84 EE239 pKa = 3.79 EE240 pKa = 3.54 AAYY243 pKa = 8.15 RR244 pKa = 11.84 TNPKK248 pKa = 9.95 VEE250 pKa = 4.3 TTVANYY256 pKa = 10.0 PEE258 pKa = 5.28 AEE260 pKa = 4.07 LRR262 pKa = 11.84 RR263 pKa = 11.84 QIRR266 pKa = 11.84 HH267 pKa = 5.81 FLTTEE272 pKa = 3.16 IRR274 pKa = 11.84 RR275 pKa = 11.84 EE276 pKa = 3.83 DD277 pKa = 3.5 DD278 pKa = 3.46 SYY280 pKa = 11.78 TVAFPSLEE288 pKa = 4.13 EE289 pKa = 3.92 HH290 pKa = 5.94 WQSRR294 pKa = 11.84 WLWAVNGAHH303 pKa = 6.59 SNIATSHH310 pKa = 4.66 VVAEE314 pKa = 4.2 EE315 pKa = 4.08 RR316 pKa = 11.84 PPGVTRR322 pKa = 11.84 EE323 pKa = 3.99 HH324 pKa = 5.87 RR325 pKa = 11.84 RR326 pKa = 11.84 AWLEE330 pKa = 3.69 RR331 pKa = 11.84 VRR333 pKa = 11.84 DD334 pKa = 4.09 DD335 pKa = 5.68 PRR337 pKa = 11.84 PAWDD341 pKa = 4.49 GYY343 pKa = 8.38 TVASGSPKK351 pKa = 10.35 LEE353 pKa = 3.84 QGKK356 pKa = 7.6 TRR358 pKa = 11.84 AIFACDD364 pKa = 3.2 TLNYY368 pKa = 10.52 LSFEE372 pKa = 4.21 HH373 pKa = 6.98 LMAPIEE379 pKa = 4.13 KK380 pKa = 8.93 RR381 pKa = 11.84 WRR383 pKa = 11.84 HH384 pKa = 4.8 EE385 pKa = 4.6 RR386 pKa = 11.84 IILDD390 pKa = 3.64 PGRR393 pKa = 11.84 GGAAGMIFRR402 pKa = 11.84 TKK404 pKa = 10.36 AARR407 pKa = 11.84 EE408 pKa = 3.89 RR409 pKa = 11.84 AGVSMMLDD417 pKa = 3.13 YY418 pKa = 11.56 DD419 pKa = 4.41 DD420 pKa = 6.4 FNSQHH425 pKa = 6.76 SNVAMKK431 pKa = 10.35 ILIEE435 pKa = 4.13 EE436 pKa = 4.39 TCALTNYY443 pKa = 9.83 PPDD446 pKa = 3.32 LAARR450 pKa = 11.84 LVEE453 pKa = 5.0 SFDD456 pKa = 3.27 KK457 pKa = 10.95 TDD459 pKa = 3.09 IYY461 pKa = 11.38 VGGVKK466 pKa = 10.11 VGRR469 pKa = 11.84 SRR471 pKa = 11.84 GTLMSGHH478 pKa = 6.99 RR479 pKa = 11.84 CTTYY483 pKa = 10.57 INSILNAVYY492 pKa = 10.45 LRR494 pKa = 11.84 LEE496 pKa = 4.58 LGDD499 pKa = 3.98 EE500 pKa = 4.16 LFDD503 pKa = 4.53 RR504 pKa = 11.84 SVSLHH509 pKa = 6.24 VGDD512 pKa = 5.33 DD513 pKa = 3.65 VYY515 pKa = 11.61 LGVASYY521 pKa = 11.87 ADD523 pKa = 3.24 ATRR526 pKa = 11.84 VQEE529 pKa = 4.74 RR530 pKa = 11.84 ISSSQLRR537 pKa = 11.84 MNPIKK542 pKa = 10.54 QSVGHH547 pKa = 6.15 VSTEE551 pKa = 3.88 FLRR554 pKa = 11.84 VACQGRR560 pKa = 11.84 DD561 pKa = 3.27 CYY563 pKa = 11.22 GYY565 pKa = 9.83 FARR568 pKa = 11.84 AVASTVSGNWVSDD581 pKa = 3.22 ILLGPLEE588 pKa = 4.85 ALTSICAASRR598 pKa = 11.84 TLVNRR603 pKa = 11.84 SGAPGLPLLLLKK615 pKa = 10.74 SVIRR619 pKa = 11.84 MTKK622 pKa = 10.31 LPRR625 pKa = 11.84 EE626 pKa = 4.1 DD627 pKa = 3.85 HH628 pKa = 7.34 DD629 pKa = 5.28 RR630 pKa = 11.84 LAQLLLGNAALGNGPQYY647 pKa = 10.81 ATGATYY653 pKa = 10.52 RR654 pKa = 11.84 SIRR657 pKa = 11.84 ATVIRR662 pKa = 11.84 SEE664 pKa = 4.38 PDD666 pKa = 2.74 DD667 pKa = 4.0 HH668 pKa = 7.13 GYY670 pKa = 11.14 SKK672 pKa = 11.35 LPMAATTTYY681 pKa = 9.49 LSSMMTPLEE690 pKa = 4.31 TTVLTEE696 pKa = 4.8 AGISPLNPMVEE707 pKa = 4.15 ASWGKK712 pKa = 7.56 TLAEE716 pKa = 4.08 PSRR719 pKa = 11.84 VVEE722 pKa = 4.19 RR723 pKa = 11.84 LRR725 pKa = 11.84 LRR727 pKa = 11.84 PVRR730 pKa = 11.84 QSLAVGSDD738 pKa = 3.07 TVEE741 pKa = 3.73 RR742 pKa = 11.84 LINQTPVKK750 pKa = 10.28 GVLTDD755 pKa = 3.49 YY756 pKa = 10.83 PILYY760 pKa = 9.51 LVKK763 pKa = 10.65 DD764 pKa = 4.11 RR765 pKa = 11.84 LTGSQLRR772 pKa = 11.84 RR773 pKa = 11.84 AIGLAGGDD781 pKa = 3.67 ANAVDD786 pKa = 4.84 LQLEE790 pKa = 4.19 AWGEE794 pKa = 3.96 RR795 pKa = 11.84 SHH797 pKa = 7.06 GCIIASPMDD806 pKa = 3.21 WATASTYY813 pKa = 10.6 GKK815 pKa = 8.35 RR816 pKa = 11.84 THH818 pKa = 6.93 ASVLTCHH825 pKa = 6.66 LRR827 pKa = 11.84 LFII830 pKa = 5.47
Molecular weight: 90.91 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.208
IPC2_protein 8.273
IPC_protein 8.185
Toseland 8.244
ProMoST 8.639
Dawson 8.887
Bjellqvist 9.019
Wikipedia 8.975
Rodwell 8.916
Grimsley 8.448
Solomon 8.96
Lehninger 8.931
Nozaki 9.048
DTASelect 8.829
Thurlkill 8.858
EMBOSS 9.019
Sillero 9.107
Patrickios 4.495
IPC_peptide 8.946
IPC2_peptide 7.966
IPC2.peptide.svr19 7.892
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1618
788
830
809.0
86.53
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.288 ± 1.511
1.112 ± 0.067
4.944 ± 0.091
4.697 ± 0.263
3.028 ± 0.798
8.776 ± 1.122
2.472 ± 0.22
3.956 ± 0.19
2.04 ± 0.531
8.9 ± 1.759
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.792 ± 0.273
3.337 ± 0.026
7.108 ± 1.31
2.781 ± 0.182
7.478 ± 0.693
6.737 ± 0.444
6.922 ± 0.135
6.551 ± 0.142
1.112 ± 0.329
2.967 ± 0.383
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here