Tortoise microvirus 93

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 7.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4V1FW19|A0A4V1FW19_9VIRU Uncharacterized protein OS=Tortoise microvirus 93 OX=2583202 PE=4 SV=1
MM1 pKa = 7.71PANEE5 pKa = 4.29PRR7 pKa = 11.84TIAGRR12 pKa = 11.84YY13 pKa = 7.26SLRR16 pKa = 11.84TPYY19 pKa = 10.93NFVLGQQPYY28 pKa = 10.29DD29 pKa = 3.68YY30 pKa = 10.27EE31 pKa = 4.36EE32 pKa = 4.76VYY34 pKa = 10.87GPSEE38 pKa = 4.15TVPDD42 pKa = 3.63QTLSLQEE49 pKa = 3.32ILYY52 pKa = 9.22RR53 pKa = 11.84HH54 pKa = 5.9SRR56 pKa = 11.84GLPIPSLSGTYY67 pKa = 9.96SDD69 pKa = 5.29EE70 pKa = 4.48DD71 pKa = 4.07LPDD74 pKa = 4.24LRR76 pKa = 11.84TLDD79 pKa = 3.47LSEE82 pKa = 3.78IQEE85 pKa = 3.95IKK87 pKa = 10.21EE88 pKa = 3.99RR89 pKa = 11.84NIDD92 pKa = 3.85RR93 pKa = 11.84IAQLRR98 pKa = 11.84DD99 pKa = 3.39EE100 pKa = 4.49IQQAEE105 pKa = 4.25LVQRR109 pKa = 11.84LKK111 pKa = 10.95RR112 pKa = 11.84EE113 pKa = 3.84EE114 pKa = 3.95QKK116 pKa = 10.86RR117 pKa = 11.84KK118 pKa = 10.07SLQDD122 pKa = 3.37ALKK125 pKa = 10.39AEE127 pKa = 4.63SGLTPDD133 pKa = 3.7QTLNPQTT140 pKa = 3.85

Molecular weight: 16.13 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4P8W734|A0A4P8W734_9VIRU DNA pilot protein OS=Tortoise microvirus 93 OX=2583202 PE=4 SV=1
MM1 pKa = 7.2RR2 pKa = 11.84QSPRR6 pKa = 11.84RR7 pKa = 11.84AWRR10 pKa = 11.84SRR12 pKa = 11.84AAPGLKK18 pKa = 9.91QPTQTYY24 pKa = 7.52EE25 pKa = 3.94VPSYY29 pKa = 10.69STTLNNAKK37 pKa = 9.66IRR39 pKa = 11.84ILLEE43 pKa = 4.01RR44 pKa = 11.84GGEE47 pKa = 4.13AEE49 pKa = 5.03GEE51 pKa = 4.2GSPLKK56 pKa = 10.45CAAGHH61 pKa = 5.88EE62 pKa = 4.4PQTRR66 pKa = 11.84AGPTFQAGWNPSVGDD81 pKa = 3.77AGTNSTNNLLIYY93 pKa = 10.61

Molecular weight: 10.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

1353

93

509

270.6

30.64

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.5 ± 1.229

0.961 ± 0.666

5.248 ± 0.645

5.987 ± 0.784

3.548 ± 1.018

5.691 ± 0.811

2.439 ± 0.662

5.765 ± 0.32

5.026 ± 1.034

8.204 ± 0.986

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.922 ± 0.248

5.248 ± 0.826

5.987 ± 0.575

5.765 ± 0.798

7.243 ± 1.027

7.021 ± 0.61

5.691 ± 0.699

4.287 ± 0.792

1.035 ± 0.228

4.435 ± 0.78
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski