Amino acid dipepetide frequency for Pseudogymnoascus sp. VKM F-4520 (FW-2644)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	9.935AlaAla: 9.935 ± 0.078	1.092AlaCys: 1.092 ± 0.016	4.459AlaAsp: 4.459 ± 0.03	5.395AlaGlu: 5.395 ± 0.042	3.145AlaPhe: 3.145 ± 0.026	6.169AlaGly: 6.169 ± 0.036	1.753AlaHis: 1.753 ± 0.02	4.651AlaIle: 4.651 ± 0.035	4.397AlaLys: 4.397 ± 0.036	7.953AlaLeu: 7.953 ± 0.047	2.041AlaMet: 2.041 ± 0.021	3.082AlaAsn: 3.082 ± 0.024	5.078AlaPro: 5.078 ± 0.048	3.413AlaGln: 3.413 ± 0.028	4.809AlaArg: 4.809 ± 0.036	7.215AlaSer: 7.215 ± 0.039	5.62AlaThr: 5.62 ± 0.036	5.648AlaVal: 5.648 ± 0.037	1.141AlaTrp: 1.141 ± 0.016	2.236AlaTyr: 2.236 ± 0.021	0.0AlaXaa: 0.0 ± 0.0
Cys	0.976CysAla: 0.976 ± 0.015	0.243CysCys: 0.243 ± 0.007	0.669CysAsp: 0.669 ± 0.013	0.6CysGlu: 0.6 ± 0.01	0.524CysPhe: 0.524 ± 0.01	1.001CysGly: 1.001 ± 0.017	0.327CysHis: 0.327 ± 0.008	0.724CysIle: 0.724 ± 0.012	0.523CysLys: 0.523 ± 0.01	1.195CysLeu: 1.195 ± 0.016	0.274CysMet: 0.274 ± 0.007	0.442CysAsn: 0.442 ± 0.009	0.615CysPro: 0.615 ± 0.012	0.429CysGln: 0.429 ± 0.009	0.697CysArg: 0.697 ± 0.013	0.911CysSer: 0.911 ± 0.017	0.7CysThr: 0.7 ± 0.014	0.772CysVal: 0.772 ± 0.014	0.205CysTrp: 0.205 ± 0.007	0.361CysTyr: 0.361 ± 0.008	0.0CysXaa: 0.0 ± 0.0
Asp	4.889AspAla: 4.889 ± 0.034	0.615AspCys: 0.615 ± 0.012	4.051AspAsp: 4.051 ± 0.036	4.464AspGlu: 4.464 ± 0.038	2.208AspPhe: 2.208 ± 0.023	4.321AspGly: 4.321 ± 0.034	1.146AspHis: 1.146 ± 0.017	3.23AspIle: 3.23 ± 0.027	2.424AspLys: 2.424 ± 0.022	4.912AspLeu: 4.912 ± 0.032	1.214AspMet: 1.214 ± 0.015	2.022AspAsn: 2.022 ± 0.022	3.052AspPro: 3.052 ± 0.023	1.738AspGln: 1.738 ± 0.016	2.863AspArg: 2.863 ± 0.026	4.054AspSer: 4.054 ± 0.028	2.991AspThr: 2.991 ± 0.025	3.738AspVal: 3.738 ± 0.029	0.881AspTrp: 0.881 ± 0.013	1.604AspTyr: 1.604 ± 0.018	0.0AspXaa: 0.0 ± 0.0
Glu	5.763GluAla: 5.763 ± 0.047	0.622GluCys: 0.622 ± 0.012	4.202GluAsp: 4.202 ± 0.035	5.648GluGlu: 5.648 ± 0.052	1.977GluPhe: 1.977 ± 0.02	4.288GluGly: 4.288 ± 0.033	1.275GluHis: 1.275 ± 0.015	3.184GluIle: 3.184 ± 0.028	3.626GluLys: 3.626 ± 0.033	5.171GluLeu: 5.171 ± 0.029	1.506GluMet: 1.506 ± 0.017	2.258GluAsn: 2.258 ± 0.02	2.671GluPro: 2.671 ± 0.033	2.321GluGln: 2.321 ± 0.026	3.904GluArg: 3.904 ± 0.035	4.265GluSer: 4.265 ± 0.032	3.449GluThr: 3.449 ± 0.027	3.841GluVal: 3.841 ± 0.035	0.913GluTrp: 0.913 ± 0.016	1.7GluTyr: 1.7 ± 0.016	0.0GluXaa: 0.0 ± 0.0
Phe	3.081PheAla: 3.081 ± 0.024	0.558PheCys: 0.558 ± 0.01	2.244PheAsp: 2.244 ± 0.022	2.148PheGlu: 2.148 ± 0.02	1.552PhePhe: 1.552 ± 0.02	2.93PheGly: 2.93 ± 0.029	0.831PheHis: 0.831 ± 0.013	1.873PheIle: 1.873 ± 0.021	1.58PheLys: 1.58 ± 0.018	3.294PheLeu: 3.294 ± 0.025	0.794PheMet: 0.794 ± 0.013	1.449PheAsn: 1.449 ± 0.016	1.883PhePro: 1.883 ± 0.022	1.379PheGln: 1.379 ± 0.019	1.809PheArg: 1.809 ± 0.017	2.893PheSer: 2.893 ± 0.021	2.153PheThr: 2.153 ± 0.021	2.37PheVal: 2.37 ± 0.024	0.632PheTrp: 0.632 ± 0.011	1.111PheTyr: 1.111 ± 0.015	0.0PheXaa: 0.0 ± 0.0
Gly	5.963GlyAla: 5.963 ± 0.039	0.945GlyCys: 0.945 ± 0.015	3.855GlyAsp: 3.855 ± 0.027	4.088GlyGlu: 4.088 ± 0.028	2.893GlyPhe: 2.893 ± 0.028	6.728GlyGly: 6.728 ± 0.071	1.729GlyHis: 1.729 ± 0.02	3.807GlyIle: 3.807 ± 0.033	3.791GlyLys: 3.791 ± 0.032	6.213GlyLeu: 6.213 ± 0.035	1.689GlyMet: 1.689 ± 0.02	2.714GlyAsn: 2.714 ± 0.026	3.316GlyPro: 3.316 ± 0.028	2.593GlyGln: 2.593 ± 0.025	4.165GlyArg: 4.165 ± 0.034	5.772GlySer: 5.772 ± 0.041	4.169GlyThr: 4.169 ± 0.033	4.702GlyVal: 4.702 ± 0.031	1.229GlyTrp: 1.229 ± 0.016	2.183GlyTyr: 2.183 ± 0.022	0.0GlyXaa: 0.0 ± 0.0
His	1.756HisAla: 1.756 ± 0.02	0.309HisCys: 0.309 ± 0.008	1.23HisAsp: 1.23 ± 0.016	1.271HisGlu: 1.271 ± 0.015	0.899HisPhe: 0.899 ± 0.012	1.693HisGly: 1.693 ± 0.018	0.752HisHis: 0.752 ± 0.014	1.258HisIle: 1.258 ± 0.014	0.92HisLys: 0.92 ± 0.016	2.116HisLeu: 2.116 ± 0.02	0.447HisMet: 0.447 ± 0.008	0.87HisAsn: 0.87 ± 0.013	1.54HisPro: 1.54 ± 0.018	0.899HisGln: 0.899 ± 0.015	1.39HisArg: 1.39 ± 0.017	1.717HisSer: 1.717 ± 0.018	1.28HisThr: 1.28 ± 0.018	1.361HisVal: 1.361 ± 0.013	0.334HisTrp: 0.334 ± 0.009	0.681HisTyr: 0.681 ± 0.012	0.0HisXaa: 0.0 ± 0.0
Ile	4.513IleAla: 4.513 ± 0.037	0.773IleCys: 0.773 ± 0.014	2.995IleAsp: 2.995 ± 0.027	3.024IleGlu: 3.024 ± 0.024	2.054IlePhe: 2.054 ± 0.021	3.461IleGly: 3.461 ± 0.028	1.173IleHis: 1.173 ± 0.017	2.782IleIle: 2.782 ± 0.027	2.391IleLys: 2.391 ± 0.024	4.667IleLeu: 4.667 ± 0.032	1.076IleMet: 1.076 ± 0.015	1.962IleAsn: 1.962 ± 0.021	3.103IlePro: 3.103 ± 0.025	1.943IleGln: 1.943 ± 0.021	2.74IleArg: 2.74 ± 0.023	4.077IleSer: 4.077 ± 0.026	3.088IleThr: 3.088 ± 0.026	3.312IleVal: 3.312 ± 0.026	0.738IleTrp: 0.738 ± 0.011	1.513IleTyr: 1.513 ± 0.018	0.0IleXaa: 0.0 ± 0.0
Lys	4.548LysAla: 4.548 ± 0.034	0.524LysCys: 0.524 ± 0.009	2.813LysAsp: 2.813 ± 0.025	3.494LysGlu: 3.494 ± 0.034	1.537LysPhe: 1.537 ± 0.016	3.242LysGly: 3.242 ± 0.029	1.103LysHis: 1.103 ± 0.014	2.4LysIle: 2.4 ± 0.022	3.272LysLys: 3.272 ± 0.048	4.238LysLeu: 4.238 ± 0.035	1.025LysMet: 1.025 ± 0.014	1.792LysAsn: 1.792 ± 0.02	2.65LysPro: 2.65 ± 0.026	1.744LysGln: 1.744 ± 0.022	3.245LysArg: 3.245 ± 0.03	3.628LysSer: 3.628 ± 0.029	2.921LysThr: 2.921 ± 0.024	2.978LysVal: 2.978 ± 0.027	0.687LysTrp: 0.687 ± 0.011	1.438LysTyr: 1.438 ± 0.016	0.0LysXaa: 0.0 ± 0.0
Leu	7.878LeuAla: 7.878 ± 0.045	1.195LeuCys: 1.195 ± 0.016	5.051LeuAsp: 5.051 ± 0.032	5.526LeuGlu: 5.526 ± 0.041	3.218LeuPhe: 3.218 ± 0.027	6.083LeuGly: 6.083 ± 0.036	2.126LeuHis: 2.126 ± 0.023	4.015LeuIle: 4.015 ± 0.035	4.301LeuLys: 4.301 ± 0.03	8.352LeuLeu: 8.352 ± 0.055	1.729LeuMet: 1.729 ± 0.02	3.128LeuAsn: 3.128 ± 0.025	5.342LeuPro: 5.342 ± 0.039	3.802LeuGln: 3.802 ± 0.033	5.418LeuArg: 5.418 ± 0.038	7.193LeuSer: 7.193 ± 0.043	4.822LeuThr: 4.822 ± 0.033	5.33LeuVal: 5.33 ± 0.033	1.213LeuTrp: 1.213 ± 0.017	2.347LeuTyr: 2.347 ± 0.021	0.0LeuXaa: 0.0 ± 0.0
Met	2.227MetAla: 2.227 ± 0.021	0.224MetCys: 0.224 ± 0.006	1.257MetAsp: 1.257 ± 0.015	1.316MetGlu: 1.316 ± 0.018	0.754MetPhe: 0.754 ± 0.012	1.572MetGly: 1.572 ± 0.017	0.479MetHis: 0.479 ± 0.009	1.006MetIle: 1.006 ± 0.015	1.046MetLys: 1.046 ± 0.016	1.824MetLeu: 1.824 ± 0.02	0.569MetMet: 0.569 ± 0.01	0.771MetAsn: 0.771 ± 0.012	1.201MetPro: 1.201 ± 0.015	0.821MetGln: 0.821 ± 0.015	1.239MetArg: 1.239 ± 0.016	1.818MetSer: 1.818 ± 0.019	1.218MetThr: 1.218 ± 0.016	1.336MetVal: 1.336 ± 0.015	0.263MetTrp: 0.263 ± 0.007	0.511MetTyr: 0.511 ± 0.011	0.0MetXaa: 0.0 ± 0.0
Asn	3.213AsnAla: 3.213 ± 0.027	0.465AsnCys: 0.465 ± 0.009	1.956AsnAsp: 1.956 ± 0.02	2.058AsnGlu: 2.058 ± 0.019	1.427AsnPhe: 1.427 ± 0.016	3.183AsnGly: 3.183 ± 0.034	0.82AsnHis: 0.82 ± 0.012	2.234AsnIle: 2.234 ± 0.022	1.589AsnLys: 1.589 ± 0.018	3.247AsnLeu: 3.247 ± 0.023	0.845AsnMet: 0.845 ± 0.013	1.575AsnAsn: 1.575 ± 0.02	2.451AsnPro: 2.451 ± 0.023	1.311AsnGln: 1.311 ± 0.016	1.886AsnArg: 1.886 ± 0.022	2.911AsnSer: 2.911 ± 0.027	2.263AsnThr: 2.263 ± 0.023	2.322AsnVal: 2.322 ± 0.023	0.585AsnTrp: 0.585 ± 0.011	1.11AsnTyr: 1.11 ± 0.016	0.0AsnXaa: 0.0 ± 0.0
Pro	5.185ProAla: 5.185 ± 0.044	0.471ProCys: 0.471 ± 0.009	2.965ProAsp: 2.965 ± 0.026	3.629ProGlu: 3.629 ± 0.03	1.927ProPhe: 1.927 ± 0.02	3.83ProGly: 3.83 ± 0.029	1.274ProHis: 1.274 ± 0.017	2.655ProIle: 2.655 ± 0.026	2.698ProLys: 2.698 ± 0.025	4.725ProLeu: 4.725 ± 0.032	1.026ProMet: 1.026 ± 0.014	2.228ProAsn: 2.228 ± 0.024	4.748ProPro: 4.748 ± 0.053	2.396ProGln: 2.396 ± 0.031	3.222ProArg: 3.222 ± 0.032	5.593ProSer: 5.593 ± 0.042	4.148ProThr: 4.148 ± 0.036	3.458ProVal: 3.458 ± 0.03	0.714ProTrp: 0.714 ± 0.011	1.453ProTyr: 1.453 ± 0.018	0.0ProXaa: 0.0 ± 0.0
Gln	3.383GlnAla: 3.383 ± 0.036	0.42GlnCys: 0.42 ± 0.01	1.955GlnAsp: 1.955 ± 0.022	2.329GlnGlu: 2.329 ± 0.022	1.244GlnPhe: 1.244 ± 0.015	2.515GlnGly: 2.515 ± 0.026	1.0GlnHis: 1.0 ± 0.016	1.892GlnIle: 1.892 ± 0.021	1.927GlnLys: 1.927 ± 0.022	3.389GlnLeu: 3.389 ± 0.03	0.83GlnMet: 0.83 ± 0.014	1.558GlnAsn: 1.558 ± 0.019	2.346GlnPro: 2.346 ± 0.027	2.345GlnGln: 2.345 ± 0.061	2.505GlnArg: 2.505 ± 0.026	2.923GlnSer: 2.923 ± 0.03	2.271GlnThr: 2.271 ± 0.022	2.083GlnVal: 2.083 ± 0.019	0.571GlnTrp: 0.571 ± 0.01	1.177GlnTyr: 1.177 ± 0.016	0.0GlnXaa: 0.0 ± 0.0
Arg	4.721ArgAla: 4.721 ± 0.029	0.688ArgCys: 0.688 ± 0.012	3.288ArgAsp: 3.288 ± 0.029	3.777ArgGlu: 3.777 ± 0.035	1.944ArgPhe: 1.944 ± 0.02	3.883ArgGly: 3.883 ± 0.035	1.471ArgHis: 1.471 ± 0.017	2.86ArgIle: 2.86 ± 0.026	3.266ArgLys: 3.266 ± 0.029	5.067ArgLeu: 5.067 ± 0.033	1.218ArgMet: 1.218 ± 0.016	2.188ArgAsn: 2.188 ± 0.023	3.203ArgPro: 3.203 ± 0.03	2.436ArgGln: 2.436 ± 0.023	4.711ArgArg: 4.711 ± 0.045	4.374ArgSer: 4.374 ± 0.04	3.16ArgThr: 3.16 ± 0.025	3.28ArgVal: 3.28 ± 0.026	0.853ArgTrp: 0.853 ± 0.013	1.535ArgTyr: 1.535 ± 0.018	0.0ArgXaa: 0.0 ± 0.0
Ser	6.637SerAla: 6.637 ± 0.033	0.881SerCys: 0.881 ± 0.013	4.139SerAsp: 4.139 ± 0.03	4.08SerGlu: 4.08 ± 0.03	2.933SerPhe: 2.933 ± 0.024	5.704SerGly: 5.704 ± 0.032	1.827SerHis: 1.827 ± 0.019	4.201SerIle: 4.201 ± 0.032	3.788SerLys: 3.788 ± 0.031	7.022SerLeu: 7.022 ± 0.041	1.652SerMet: 1.652 ± 0.019	3.094SerAsn: 3.094 ± 0.027	5.25SerPro: 5.25 ± 0.052	3.134SerGln: 3.134 ± 0.028	4.647SerArg: 4.647 ± 0.037	8.039SerSer: 8.039 ± 0.062	5.579SerThr: 5.579 ± 0.041	4.536SerVal: 4.536 ± 0.027	1.112SerTrp: 1.112 ± 0.014	2.107SerTyr: 2.107 ± 0.02	0.0SerXaa: 0.0 ± 0.0
Thr	5.494ThrAla: 5.494 ± 0.038	0.752ThrCys: 0.752 ± 0.013	2.901ThrAsp: 2.901 ± 0.025	3.177ThrGlu: 3.177 ± 0.028	2.238ThrPhe: 2.238 ± 0.022	4.358ThrGly: 4.358 ± 0.032	1.26ThrHis: 1.26 ± 0.015	3.292ThrIle: 3.292 ± 0.024	2.732ThrLys: 2.732 ± 0.023	5.26ThrLeu: 5.26 ± 0.035	1.222ThrMet: 1.222 ± 0.014	2.221ThrAsn: 2.221 ± 0.024	4.376ThrPro: 4.376 ± 0.035	2.039ThrGln: 2.039 ± 0.022	2.978ThrArg: 2.978 ± 0.027	5.361ThrSer: 5.361 ± 0.039	4.53ThrThr: 4.53 ± 0.043	3.793ThrVal: 3.793 ± 0.03	0.887ThrTrp: 0.887 ± 0.013	1.656ThrTyr: 1.656 ± 0.017	0.0ThrXaa: 0.0 ± 0.0
Val	5.676ValAla: 5.676 ± 0.035	0.792ValCys: 0.792 ± 0.013	3.82ValAsp: 3.82 ± 0.029	4.155ValGlu: 4.155 ± 0.033	2.366ValPhe: 2.366 ± 0.024	4.412ValGly: 4.412 ± 0.035	1.32ValHis: 1.32 ± 0.016	3.089ValIle: 3.089 ± 0.024	3.019ValLys: 3.019 ± 0.026	5.515ValLeu: 5.515 ± 0.04	1.328ValMet: 1.328 ± 0.015	2.254ValAsn: 2.254 ± 0.022	3.459ValPro: 3.459 ± 0.028	2.301ValGln: 2.301 ± 0.021	3.238ValArg: 3.238 ± 0.024	4.47ValSer: 4.47 ± 0.029	3.595ValThr: 3.595 ± 0.032	4.567ValVal: 4.567 ± 0.036	0.865ValTrp: 0.865 ± 0.013	1.717ValTyr: 1.717 ± 0.019	0.0ValXaa: 0.0 ± 0.0
Trp	1.205TrpAla: 1.205 ± 0.014	0.194TrpCys: 0.194 ± 0.007	0.906TrpAsp: 0.906 ± 0.016	0.865TrpGlu: 0.865 ± 0.013	0.542TrpPhe: 0.542 ± 0.01	1.004TrpGly: 1.004 ± 0.014	0.339TrpHis: 0.339 ± 0.008	0.758TrpIle: 0.758 ± 0.012	0.798TrpLys: 0.798 ± 0.013	1.302TrpLeu: 1.302 ± 0.016	0.365TrpMet: 0.365 ± 0.008	0.627TrpAsn: 0.627 ± 0.012	0.603TrpPro: 0.603 ± 0.01	0.535TrpGln: 0.535 ± 0.011	0.914TrpArg: 0.914 ± 0.015	1.032TrpSer: 1.032 ± 0.014	0.912TrpThr: 0.912 ± 0.014	0.929TrpVal: 0.929 ± 0.013	0.271TrpTrp: 0.271 ± 0.007	0.421TrpTyr: 0.421 ± 0.009	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.19TyrAla: 2.19 ± 0.02	0.423TyrCys: 0.423 ± 0.008	1.661TyrAsp: 1.661 ± 0.017	1.538TyrGlu: 1.538 ± 0.015	1.185TyrPhe: 1.185 ± 0.015	2.167TyrGly: 2.167 ± 0.02	0.716TyrHis: 0.716 ± 0.011	1.475TyrIle: 1.475 ± 0.018	1.19TyrLys: 1.19 ± 0.016	2.603TyrLeu: 2.603 ± 0.024	0.602TyrMet: 0.602 ± 0.01	1.218TyrAsn: 1.218 ± 0.015	1.458TyrPro: 1.458 ± 0.016	1.082TyrGln: 1.082 ± 0.016	1.545TyrArg: 1.545 ± 0.018	2.115TyrSer: 2.115 ± 0.022	1.658TyrThr: 1.658 ± 0.02	1.602TyrVal: 1.602 ± 0.016	0.433TyrTrp: 0.433 ± 0.009	0.95TyrTyr: 0.95 ± 0.014	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 11550 proteins (5669913 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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