Halorubrum halodurans

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Haloferacales; Halorubraceae; Halorubrum

Average proteome isoelectric point is 4.91

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3137 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A256IR82|A0A256IR82_9EURY Cytochrome C oxidase subunit I (Fragment) OS=Halorubrum halodurans OX=1383851 GN=DJ70_02010 PE=3 SV=1
MM1 pKa = 7.59SSPAGTAGEE10 pKa = 4.17DD11 pKa = 3.88GEE13 pKa = 4.55RR14 pKa = 11.84AEE16 pKa = 5.48HH17 pKa = 6.99NDD19 pKa = 4.23DD20 pKa = 4.37EE21 pKa = 6.13PGDD24 pKa = 4.26DD25 pKa = 3.49EE26 pKa = 4.81TVDD29 pKa = 3.74VDD31 pKa = 3.85LQRR34 pKa = 11.84CEE36 pKa = 3.67VCGAEE41 pKa = 4.47KK42 pKa = 10.09PADD45 pKa = 3.92LVDD48 pKa = 3.25HH49 pKa = 5.86TTIEE53 pKa = 4.47TIAPMEE59 pKa = 4.18ADD61 pKa = 2.86ICEE64 pKa = 4.57SCQHH68 pKa = 4.9VHH70 pKa = 5.82NHH72 pKa = 5.51QLDD75 pKa = 3.55EE76 pKa = 4.4DD77 pKa = 4.17VCMKK81 pKa = 10.54CGSEE85 pKa = 3.72TDD87 pKa = 3.39GAGFYY92 pKa = 11.21LEE94 pKa = 5.26LEE96 pKa = 4.44YY97 pKa = 10.55PLGAEE102 pKa = 4.35DD103 pKa = 5.69LPAKK107 pKa = 10.27RR108 pKa = 11.84AGSLCGEE115 pKa = 4.26CADD118 pKa = 5.44WIGTDD123 pKa = 2.86IAYY126 pKa = 8.86RR127 pKa = 11.84ALDD130 pKa = 3.88ADD132 pKa = 4.07EE133 pKa = 5.2EE134 pKa = 4.48ATQEE138 pKa = 4.74LVRR141 pKa = 11.84LLDD144 pKa = 3.51KK145 pKa = 11.26HH146 pKa = 7.12HH147 pKa = 7.43DD148 pKa = 3.92AQSTDD153 pKa = 3.54QPDD156 pKa = 4.03SEE158 pKa = 6.15DD159 pKa = 3.94DD160 pKa = 3.77DD161 pKa = 4.23

Molecular weight: 17.48 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A256IPX4|A0A256IPX4_9EURY Uncharacterized protein OS=Halorubrum halodurans OX=1383851 GN=DJ70_02575 PE=4 SV=1
MM1 pKa = 7.7ASFDD5 pKa = 3.47AAEE8 pKa = 4.21RR9 pKa = 11.84RR10 pKa = 11.84NLDD13 pKa = 2.96RR14 pKa = 11.84QICMRR19 pKa = 11.84CNARR23 pKa = 11.84NAPDD27 pKa = 3.43AGRR30 pKa = 11.84CRR32 pKa = 11.84KK33 pKa = 9.7CGYY36 pKa = 8.58TKK38 pKa = 10.64LRR40 pKa = 11.84PKK42 pKa = 10.52AKK44 pKa = 9.61EE45 pKa = 3.4RR46 pKa = 11.84RR47 pKa = 11.84AAA49 pKa = 3.56

Molecular weight: 5.63 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3137

0

3137

917458

27

4473

292.5

31.55

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.819 ± 0.078

0.663 ± 0.014

8.744 ± 0.052

8.464 ± 0.061

3.165 ± 0.028

9.077 ± 0.045

1.936 ± 0.023

3.728 ± 0.033

1.626 ± 0.024

8.62 ± 0.058

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.593 ± 0.017

2.168 ± 0.03

4.88 ± 0.031

1.887 ± 0.027

7.106 ± 0.062

5.223 ± 0.036

6.32 ± 0.056

9.349 ± 0.053

1.061 ± 0.017

2.57 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski