Micromonospora sp. Llam0

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micromonosporales; Micromonosporaceae; Micromonospora; unclassified Micromonospora

Average proteome isoelectric point is 6.56

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7541 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3N1B2S3|A0A3N1B2S3_9ACTN DNA-binding XRE family transcriptional regulator OS=Micromonospora sp. Llam0 OX=2485143 GN=EDC02_5069 PE=4 SV=1
MM1 pKa = 7.45LNAAGWGRR9 pKa = 11.84LGAGTLLAGALVVAAAAPAAAQNPGPDD36 pKa = 3.55LFVSFDD42 pKa = 3.79RR43 pKa = 11.84EE44 pKa = 3.95PVAEE48 pKa = 4.06VDD50 pKa = 3.5NSGVTLGMYY59 pKa = 9.58VYY61 pKa = 10.74NYY63 pKa = 10.34GDD65 pKa = 3.79APASDD70 pKa = 3.57VTIVVDD76 pKa = 3.48ATGVDD81 pKa = 3.9DD82 pKa = 5.36AVQLAEE88 pKa = 5.15GYY90 pKa = 10.48HH91 pKa = 6.22PGCEE95 pKa = 3.6ITDD98 pKa = 3.6RR99 pKa = 11.84VVTCAYY105 pKa = 9.39GALAAGLTDD114 pKa = 4.14HH115 pKa = 7.46IYY117 pKa = 10.17PVRR120 pKa = 11.84LASRR124 pKa = 11.84AGATPGDD131 pKa = 4.3AGTMTVTISSAEE143 pKa = 3.84QDD145 pKa = 3.9ANPADD150 pKa = 3.81NTTTFPVTVLASGPDD165 pKa = 3.7LVALVDD171 pKa = 5.66DD172 pKa = 5.03INTEE176 pKa = 3.83QDD178 pKa = 2.92RR179 pKa = 11.84VGPGDD184 pKa = 3.59TAPLYY189 pKa = 10.79AAVLNEE195 pKa = 4.2GDD197 pKa = 4.02STADD201 pKa = 3.06SYY203 pKa = 11.73TIGLDD208 pKa = 3.23LPTGVGFVDD217 pKa = 5.25EE218 pKa = 4.82YY219 pKa = 10.97TDD221 pKa = 4.25CDD223 pKa = 4.48YY224 pKa = 11.34ISYY227 pKa = 9.12WPNDD231 pKa = 3.86PKK233 pKa = 11.08TEE235 pKa = 4.07GYY237 pKa = 10.71AYY239 pKa = 10.02GPSRR243 pKa = 11.84VTCALTIPLAPGEE256 pKa = 4.26TLFLVDD262 pKa = 4.14EE263 pKa = 4.39QGEE266 pKa = 4.77SLFDD270 pKa = 4.03LYY272 pKa = 11.05FGNNLAGPDD281 pKa = 3.49QTSGSFEE288 pKa = 3.96ARR290 pKa = 11.84LVADD294 pKa = 5.6DD295 pKa = 4.37PAQAQARR302 pKa = 11.84ASTAGGKK309 pKa = 9.79SINEE313 pKa = 3.96ALANAQAAAARR324 pKa = 11.84SAGRR328 pKa = 11.84SAASLLEE335 pKa = 4.65EE336 pKa = 5.35IDD338 pKa = 3.72TEE340 pKa = 4.99DD341 pKa = 3.4NVDD344 pKa = 3.47YY345 pKa = 10.42FAVHH349 pKa = 6.3TKK351 pKa = 10.59PNTFDD356 pKa = 3.18IAVTAEE362 pKa = 4.26PVTGEE367 pKa = 3.87PGEE370 pKa = 4.41TVDD373 pKa = 4.89LTFTVVNNGPSDD385 pKa = 3.56GSGPGVTVTAPSGTVLLPSDD405 pKa = 3.06WCYY408 pKa = 11.11TEE410 pKa = 5.82GEE412 pKa = 4.31PGTRR416 pKa = 11.84LPEE419 pKa = 4.4SPALRR424 pKa = 11.84CNFEE428 pKa = 4.89SIFPATASGGGQITHH443 pKa = 6.54TLQLKK448 pKa = 10.07IEE450 pKa = 4.39STPGTDD456 pKa = 3.27GTIVADD462 pKa = 3.59SGGPSTEE469 pKa = 4.2SDD471 pKa = 3.19PSNNTVAIVFTGAGDD486 pKa = 4.48DD487 pKa = 4.41PDD489 pKa = 5.33DD490 pKa = 4.17EE491 pKa = 5.72PGGGGAGDD499 pKa = 4.05GDD501 pKa = 4.2EE502 pKa = 5.05LPITGASVGTVVGVGAVAVALGVALLLTVRR532 pKa = 11.84RR533 pKa = 11.84RR534 pKa = 11.84ATVTVSAPEE543 pKa = 3.6

Molecular weight: 55.05 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3N1A7I6|A0A3N1A7I6_9ACTN Uncharacterized protein DUF4192 OS=Micromonospora sp. Llam0 OX=2485143 GN=EDC02_7697 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILSTRR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.47GRR40 pKa = 11.84ARR42 pKa = 11.84LSAA45 pKa = 3.91

Molecular weight: 5.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7541

0

7541

2555137

29

14379

338.8

36.26

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.791 ± 0.049

0.799 ± 0.01

6.479 ± 0.023

4.702 ± 0.025

2.59 ± 0.016

9.241 ± 0.029

2.224 ± 0.014

3.323 ± 0.016

1.369 ± 0.018

10.207 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.606 ± 0.01

1.764 ± 0.016

6.43 ± 0.028

3.035 ± 0.015

8.575 ± 0.03

4.895 ± 0.019

6.356 ± 0.028

8.889 ± 0.029

1.621 ± 0.012

2.102 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski