Amino acid dipepetide frequency for Micromonospora sp. Llam0

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
23.257AlaAla: 23.257 ± 0.173
0.969AlaCys: 0.969 ± 0.022
9.765AlaAsp: 9.765 ± 0.077
7.846AlaGlu: 7.846 ± 0.07
3.131AlaPhe: 3.131 ± 0.042
14.863AlaGly: 14.863 ± 0.114
2.505AlaHis: 2.505 ± 0.032
4.028AlaIle: 4.028 ± 0.047
1.879AlaLys: 1.879 ± 0.037
13.135AlaLeu: 13.135 ± 0.107
2.352AlaMet: 2.352 ± 0.032
2.126AlaAsn: 2.126 ± 0.033
6.683AlaPro: 6.683 ± 0.076
3.756AlaGln: 3.756 ± 0.047
10.208AlaArg: 10.208 ± 0.07
5.698AlaSer: 5.698 ± 0.05
8.258AlaThr: 8.258 ± 0.072
12.936AlaVal: 12.936 ± 0.105
1.838AlaTrp: 1.838 ± 0.029
2.677AlaTyr: 2.677 ± 0.033
0.0AlaXaa: 0.0 ± 0.0
Cys
1.039CysAla: 1.039 ± 0.023
0.11CysCys: 0.11 ± 0.006
0.547CysAsp: 0.547 ± 0.017
0.314CysGlu: 0.314 ± 0.011
0.214CysPhe: 0.214 ± 0.009
0.967CysGly: 0.967 ± 0.019
0.227CysHis: 0.227 ± 0.009
0.15CysIle: 0.15 ± 0.008
0.083CysLys: 0.083 ± 0.007
0.749CysLeu: 0.749 ± 0.019
0.106CysMet: 0.106 ± 0.006
0.141CysAsn: 0.141 ± 0.007
0.539CysPro: 0.539 ± 0.017
0.226CysGln: 0.226 ± 0.009
0.733CysArg: 0.733 ± 0.02
0.402CysSer: 0.402 ± 0.014
0.457CysThr: 0.457 ± 0.014
0.661CysVal: 0.661 ± 0.015
0.14CysTrp: 0.14 ± 0.007
0.19CysTyr: 0.19 ± 0.009
0.0CysXaa: 0.0 ± 0.0
Asp
7.757AspAla: 7.757 ± 0.071
0.482AspCys: 0.482 ± 0.014
4.356AspAsp: 4.356 ± 0.049
3.812AspGlu: 3.812 ± 0.045
1.477AspPhe: 1.477 ± 0.024
7.013AspGly: 7.013 ± 0.072
1.468AspHis: 1.468 ± 0.026
1.826AspIle: 1.826 ± 0.034
0.788AspLys: 0.788 ± 0.02
6.942AspLeu: 6.942 ± 0.054
0.748AspMet: 0.748 ± 0.018
1.066AspAsn: 1.066 ± 0.023
5.476AspPro: 5.476 ± 0.053
2.208AspGln: 2.208 ± 0.036
6.22AspArg: 6.22 ± 0.056
2.625AspSer: 2.625 ± 0.042
3.217AspThr: 3.217 ± 0.034
5.017AspVal: 5.017 ± 0.052
1.089AspTrp: 1.089 ± 0.025
1.205AspTyr: 1.205 ± 0.025
0.0AspXaa: 0.0 ± 0.0
Glu
5.637GluAla: 5.637 ± 0.059
0.34GluCys: 0.34 ± 0.012
1.868GluAsp: 1.868 ± 0.028
2.079GluGlu: 2.079 ± 0.034
1.491GluPhe: 1.491 ± 0.026
2.534GluGly: 2.534 ± 0.04
1.334GluHis: 1.334 ± 0.024
2.248GluIle: 2.248 ± 0.029
0.796GluLys: 0.796 ± 0.023
6.287GluLeu: 6.287 ± 0.064
0.796GluMet: 0.796 ± 0.017
0.736GluAsn: 0.736 ± 0.018
3.494GluPro: 3.494 ± 0.048
2.344GluGln: 2.344 ± 0.034
4.284GluArg: 4.284 ± 0.05
2.268GluSer: 2.268 ± 0.035
2.452GluThr: 2.452 ± 0.039
4.29GluVal: 4.29 ± 0.047
0.685GluTrp: 0.685 ± 0.017
1.062GluTyr: 1.062 ± 0.022
0.0GluXaa: 0.0 ± 0.0
Phe
3.524PheAla: 3.524 ± 0.04
0.249PheCys: 0.249 ± 0.011
2.142PheAsp: 2.142 ± 0.032
1.148PheGlu: 1.148 ± 0.024
0.774PhePhe: 0.774 ± 0.02
2.865PheGly: 2.865 ± 0.039
0.568PheHis: 0.568 ± 0.017
0.743PheIle: 0.743 ± 0.019
0.343PheLys: 0.343 ± 0.013
2.358PheLeu: 2.358 ± 0.036
0.321PheMet: 0.321 ± 0.012
0.566PheAsn: 0.566 ± 0.017
1.286PhePro: 1.286 ± 0.025
0.668PheGln: 0.668 ± 0.018
1.757PheArg: 1.757 ± 0.028
1.311PheSer: 1.311 ± 0.027
1.909PheThr: 1.909 ± 0.032
2.355PheVal: 2.355 ± 0.031
0.421PheTrp: 0.421 ± 0.014
0.597PheTyr: 0.597 ± 0.016
0.0PheXaa: 0.0 ± 0.0
Gly
10.264GlyAla: 10.264 ± 0.078
0.87GlyCys: 0.87 ± 0.02
5.497GlyAsp: 5.497 ± 0.051
4.392GlyGlu: 4.392 ± 0.047
2.67GlyPhe: 2.67 ± 0.032
8.641GlyGly: 8.641 ± 0.085
2.212GlyHis: 2.212 ± 0.034
3.063GlyIle: 3.063 ± 0.037
1.627GlyLys: 1.627 ± 0.027
9.198GlyLeu: 9.198 ± 0.073
1.865GlyMet: 1.865 ± 0.026
1.756GlyAsn: 1.756 ± 0.03
5.575GlyPro: 5.575 ± 0.052
3.106GlyGln: 3.106 ± 0.039
8.24GlyArg: 8.24 ± 0.062
5.093GlySer: 5.093 ± 0.051
6.159GlyThr: 6.159 ± 0.064
7.759GlyVal: 7.759 ± 0.069
1.933GlyTrp: 1.933 ± 0.025
2.488GlyTyr: 2.488 ± 0.037
0.0GlyXaa: 0.0 ± 0.0
His
2.553HisAla: 2.553 ± 0.031
0.213HisCys: 0.213 ± 0.009
1.455HisAsp: 1.455 ± 0.028
0.939HisGlu: 0.939 ± 0.021
0.557HisPhe: 0.557 ± 0.016
2.147HisGly: 2.147 ± 0.032
0.707HisHis: 0.707 ± 0.018
0.579HisIle: 0.579 ± 0.016
0.231HisLys: 0.231 ± 0.01
2.529HisLeu: 2.529 ± 0.034
0.245HisMet: 0.245 ± 0.011
0.389HisAsn: 0.389 ± 0.014
1.847HisPro: 1.847 ± 0.029
0.783HisGln: 0.783 ± 0.019
2.442HisArg: 2.442 ± 0.036
0.916HisSer: 0.916 ± 0.02
1.236HisThr: 1.236 ± 0.027
1.614HisVal: 1.614 ± 0.029
0.366HisTrp: 0.366 ± 0.012
0.498HisTyr: 0.498 ± 0.014
0.0HisXaa: 0.0 ± 0.0
Ile
4.81IleAla: 4.81 ± 0.048
0.343IleCys: 0.343 ± 0.011
2.789IleAsp: 2.789 ± 0.029
1.943IleGlu: 1.943 ± 0.029
0.818IlePhe: 0.818 ± 0.018
3.668IleGly: 3.668 ± 0.038
0.608IleHis: 0.608 ± 0.017
0.998IleIle: 0.998 ± 0.021
0.545IleLys: 0.545 ± 0.015
2.473IleLeu: 2.473 ± 0.032
0.452IleMet: 0.452 ± 0.016
0.762IleAsn: 0.762 ± 0.02
1.648IlePro: 1.648 ± 0.026
0.703IleGln: 0.703 ± 0.019
2.634IleArg: 2.634 ± 0.037
1.789IleSer: 1.789 ± 0.029
2.218IleThr: 2.218 ± 0.034
2.936IleVal: 2.936 ± 0.038
0.484IleTrp: 0.484 ± 0.015
0.609IleTyr: 0.609 ± 0.017
0.0IleXaa: 0.0 ± 0.0
Lys
1.711LysAla: 1.711 ± 0.033
0.097LysCys: 0.097 ± 0.007
0.647LysAsp: 0.647 ± 0.02
0.599LysGlu: 0.599 ± 0.018
0.33LysPhe: 0.33 ± 0.014
1.009LysGly: 1.009 ± 0.025
0.283LysHis: 0.283 ± 0.011
0.71LysIle: 0.71 ± 0.02
0.348LysLys: 0.348 ± 0.016
1.508LysLeu: 1.508 ± 0.03
0.263LysMet: 0.263 ± 0.011
0.278LysAsn: 0.278 ± 0.01
0.885LysPro: 0.885 ± 0.021
0.511LysGln: 0.511 ± 0.017
1.106LysArg: 1.106 ± 0.024
0.783LysSer: 0.783 ± 0.021
0.859LysThr: 0.859 ± 0.02
1.277LysVal: 1.277 ± 0.027
0.202LysTrp: 0.202 ± 0.009
0.284LysTyr: 0.284 ± 0.011
0.0LysXaa: 0.0 ± 0.0
Leu
16.208LeuAla: 16.208 ± 0.119
0.778LeuCys: 0.778 ± 0.023
7.033LeuAsp: 7.033 ± 0.058
3.484LeuGlu: 3.484 ± 0.045
2.421LeuPhe: 2.421 ± 0.039
8.545LeuGly: 8.545 ± 0.069
2.288LeuHis: 2.288 ± 0.03
3.563LeuIle: 3.563 ± 0.045
1.127LeuLys: 1.127 ± 0.027
11.26LeuLeu: 11.26 ± 0.125
1.303LeuMet: 1.303 ± 0.024
1.592LeuAsn: 1.592 ± 0.026
6.717LeuPro: 6.717 ± 0.056
2.075LeuGln: 2.075 ± 0.03
9.418LeuArg: 9.418 ± 0.073
4.905LeuSer: 4.905 ± 0.047
7.16LeuThr: 7.16 ± 0.056
9.216LeuVal: 9.216 ± 0.07
1.282LeuTrp: 1.282 ± 0.022
1.695LeuTyr: 1.695 ± 0.028
0.0LeuXaa: 0.0 ± 0.0
Met
2.005MetAla: 2.005 ± 0.033
0.117MetCys: 0.117 ± 0.007
0.758MetAsp: 0.758 ± 0.018
0.547MetGlu: 0.547 ± 0.014
0.463MetPhe: 0.463 ± 0.014
0.975MetGly: 0.975 ± 0.021
0.313MetHis: 0.313 ± 0.012
0.764MetIle: 0.764 ± 0.017
0.27MetLys: 0.27 ± 0.012
1.717MetLeu: 1.717 ± 0.029
0.283MetMet: 0.283 ± 0.011
0.363MetAsn: 0.363 ± 0.013
1.061MetPro: 1.061 ± 0.019
0.427MetGln: 0.427 ± 0.013
1.403MetArg: 1.403 ± 0.027
1.221MetSer: 1.221 ± 0.023
1.508MetThr: 1.508 ± 0.025
1.394MetVal: 1.394 ± 0.026
0.208MetTrp: 0.208 ± 0.009
0.265MetTyr: 0.265 ± 0.011
0.0MetXaa: 0.0 ± 0.0
Asn
2.076AsnAla: 2.076 ± 0.036
0.179AsnCys: 0.179 ± 0.008
0.98AsnAsp: 0.98 ± 0.022
0.755AsnGlu: 0.755 ± 0.02
0.472AsnPhe: 0.472 ± 0.015
1.852AsnGly: 1.852 ± 0.03
0.407AsnHis: 0.407 ± 0.012
0.612AsnIle: 0.612 ± 0.016
0.289AsnLys: 0.289 ± 0.011
1.845AsnLeu: 1.845 ± 0.029
0.268AsnMet: 0.268 ± 0.012
0.443AsnAsn: 0.443 ± 0.017
1.412AsnPro: 1.412 ± 0.024
0.636AsnGln: 0.636 ± 0.015
1.476AsnArg: 1.476 ± 0.024
0.913AsnSer: 0.913 ± 0.022
0.956AsnThr: 0.956 ± 0.021
1.316AsnVal: 1.316 ± 0.025
0.322AsnTrp: 0.322 ± 0.012
0.431AsnTyr: 0.431 ± 0.014
0.0AsnXaa: 0.0 ± 0.0
Pro
10.329ProAla: 10.329 ± 0.089
0.331ProCys: 0.331 ± 0.013
5.284ProAsp: 5.284 ± 0.056
3.234ProGlu: 3.234 ± 0.04
1.373ProPhe: 1.373 ± 0.025
6.681ProGly: 6.681 ± 0.057
1.262ProHis: 1.262 ± 0.024
1.654ProIle: 1.654 ± 0.028
0.769ProLys: 0.769 ± 0.022
4.981ProLeu: 4.981 ± 0.048
0.994ProMet: 0.994 ± 0.02
0.987ProAsn: 0.987 ± 0.022
4.624ProPro: 4.624 ± 0.074
1.978ProGln: 1.978 ± 0.026
3.916ProArg: 3.916 ± 0.044
3.03ProSer: 3.03 ± 0.042
4.406ProThr: 4.406 ± 0.052
6.185ProVal: 6.185 ± 0.052
0.946ProTrp: 0.946 ± 0.022
1.334ProTyr: 1.334 ± 0.023
0.0ProXaa: 0.0 ± 0.0
Gln
4.067GlnAla: 4.067 ± 0.043
0.181GlnCys: 0.181 ± 0.009
1.15GlnAsp: 1.15 ± 0.024
1.199GlnGlu: 1.199 ± 0.024
0.784GlnPhe: 0.784 ± 0.018
1.855GlnGly: 1.855 ± 0.029
0.704GlnHis: 0.704 ± 0.018
1.299GlnIle: 1.299 ± 0.023
0.328GlnLys: 0.328 ± 0.014
3.804GlnLeu: 3.804 ± 0.047
0.508GlnMet: 0.508 ± 0.014
0.454GlnAsn: 0.454 ± 0.015
2.366GlnPro: 2.366 ± 0.032
1.424GlnGln: 1.424 ± 0.03
3.138GlnArg: 3.138 ± 0.036
1.242GlnSer: 1.242 ± 0.024
1.583GlnThr: 1.583 ± 0.028
3.133GlnVal: 3.133 ± 0.039
0.58GlnTrp: 0.58 ± 0.015
0.553GlnTyr: 0.553 ± 0.015
0.0GlnXaa: 0.0 ± 0.0
Arg
9.924ArgAla: 9.924 ± 0.075
0.759ArgCys: 0.759 ± 0.02
5.011ArgAsp: 5.011 ± 0.053
3.767ArgGlu: 3.767 ± 0.043
2.5ArgPhe: 2.5 ± 0.035
5.406ArgGly: 5.406 ± 0.049
2.457ArgHis: 2.457 ± 0.035
3.423ArgIle: 3.423 ± 0.041
1.206ArgLys: 1.206 ± 0.025
9.656ArgLeu: 9.656 ± 0.073
1.909ArgMet: 1.909 ± 0.027
1.462ArgAsn: 1.462 ± 0.027
5.655ArgPro: 5.655 ± 0.052
3.129ArgGln: 3.129 ± 0.041
9.351ArgArg: 9.351 ± 0.076
4.532ArgSer: 4.532 ± 0.045
5.208ArgThr: 5.208 ± 0.047
6.417ArgVal: 6.417 ± 0.061
1.79ArgTrp: 1.79 ± 0.026
2.184ArgTyr: 2.184 ± 0.029
0.0ArgXaa: 0.0 ± 0.0
Ser
6.58SerAla: 6.58 ± 0.061
0.418SerCys: 0.418 ± 0.014
2.753SerAsp: 2.753 ± 0.04
1.9SerGlu: 1.9 ± 0.027
1.428SerPhe: 1.428 ± 0.027
5.726SerGly: 5.726 ± 0.057
0.965SerHis: 0.965 ± 0.021
1.589SerIle: 1.589 ± 0.026
0.674SerLys: 0.674 ± 0.018
4.228SerLeu: 4.228 ± 0.046
1.038SerMet: 1.038 ± 0.021
0.88SerAsn: 0.88 ± 0.023
3.148SerPro: 3.148 ± 0.041
1.307SerGln: 1.307 ± 0.025
3.86SerArg: 3.86 ± 0.043
2.662SerSer: 2.662 ± 0.042
3.275SerThr: 3.275 ± 0.044
4.264SerVal: 4.264 ± 0.049
1.008SerTrp: 1.008 ± 0.021
1.249SerTyr: 1.249 ± 0.026
0.0SerXaa: 0.0 ± 0.0
Thr
9.228ThrAla: 9.228 ± 0.075
0.453ThrCys: 0.453 ± 0.012
4.178ThrAsp: 4.178 ± 0.044
2.967ThrGlu: 2.967 ± 0.038
1.638ThrPhe: 1.638 ± 0.031
7.125ThrGly: 7.125 ± 0.066
1.133ThrHis: 1.133 ± 0.023
2.079ThrIle: 2.079 ± 0.03
0.774ThrLys: 0.774 ± 0.018
5.599ThrLeu: 5.599 ± 0.054
1.015ThrMet: 1.015 ± 0.02
1.142ThrAsn: 1.142 ± 0.026
4.172ThrPro: 4.172 ± 0.053
1.37ThrGln: 1.37 ± 0.021
4.344ThrArg: 4.344 ± 0.044
3.215ThrSer: 3.215 ± 0.038
4.132ThrThr: 4.132 ± 0.063
6.753ThrVal: 6.753 ± 0.064
0.943ThrTrp: 0.943 ± 0.019
1.305ThrTyr: 1.305 ± 0.034
0.0ThrXaa: 0.0 ± 0.0
Val
12.487ValAla: 12.487 ± 0.092
0.728ValCys: 0.728 ± 0.019
6.419ValAsp: 6.419 ± 0.058
4.511ValGlu: 4.511 ± 0.046
2.243ValPhe: 2.243 ± 0.032
7.476ValGly: 7.476 ± 0.069
1.838ValHis: 1.838 ± 0.027
2.875ValIle: 2.875 ± 0.039
1.153ValLys: 1.153 ± 0.025
9.315ValLeu: 9.315 ± 0.081
1.128ValMet: 1.128 ± 0.021
1.726ValAsn: 1.726 ± 0.031
5.442ValPro: 5.442 ± 0.045
2.108ValGln: 2.108 ± 0.03
7.458ValArg: 7.458 ± 0.058
4.307ValSer: 4.307 ± 0.045
6.357ValThr: 6.357 ± 0.058
8.591ValVal: 8.591 ± 0.083
1.168ValTrp: 1.168 ± 0.024
1.558ValTyr: 1.558 ± 0.028
0.0ValXaa: 0.0 ± 0.0
Trp
1.657TrpAla: 1.657 ± 0.028
0.175TrpCys: 0.175 ± 0.009
0.773TrpAsp: 0.773 ± 0.021
0.59TrpGlu: 0.59 ± 0.015
0.499TrpPhe: 0.499 ± 0.014
0.948TrpGly: 0.948 ± 0.019
0.46TrpHis: 0.46 ± 0.013
0.58TrpIle: 0.58 ± 0.014
0.213TrpLys: 0.213 ± 0.009
2.059TrpLeu: 2.059 ± 0.033
0.292TrpMet: 0.292 ± 0.01
0.378TrpAsn: 0.378 ± 0.014
0.989TrpPro: 0.989 ± 0.021
0.8TrpGln: 0.8 ± 0.019
1.701TrpArg: 1.701 ± 0.027
1.119TrpSer: 1.119 ± 0.024
1.091TrpThr: 1.091 ± 0.021
1.083TrpVal: 1.083 ± 0.021
0.425TrpTrp: 0.425 ± 0.012
0.373TrpTyr: 0.373 ± 0.012
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.798TyrAla: 2.798 ± 0.035
0.201TyrCys: 0.201 ± 0.008
1.389TyrAsp: 1.389 ± 0.033
1.0TyrGlu: 1.0 ± 0.021
0.621TyrPhe: 0.621 ± 0.019
2.111TyrGly: 2.111 ± 0.032
0.506TyrHis: 0.506 ± 0.015
0.447TyrIle: 0.447 ± 0.013
0.244TyrLys: 0.244 ± 0.011
2.428TyrLeu: 2.428 ± 0.034
0.176TyrMet: 0.176 ± 0.01
0.394TyrAsn: 0.394 ± 0.014
1.28TyrPro: 1.28 ± 0.023
0.794TyrGln: 0.794 ± 0.019
2.057TyrArg: 2.057 ± 0.03
0.922TyrSer: 0.922 ± 0.022
1.12TyrThr: 1.12 ± 0.027
1.692TyrVal: 1.692 ± 0.03
0.377TyrTrp: 0.377 ± 0.012
0.465TyrTyr: 0.465 ± 0.014
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 7541 proteins (2555137 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski