Fibrisoma limi BUZ 3

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Cytophagia; Cytophagales; Cytophagaceae; Fibrisoma; Fibrisoma limi

Average proteome isoelectric point is 6.88

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6341 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|I2GCC2|I2GCC2_9BACT Response regulator receiver protein OS=Fibrisoma limi BUZ 3 OX=1185876 GN=BN8_00475 PE=4 SV=1
MM1 pKa = 7.65SVLRR5 pKa = 11.84CIAIDD10 pKa = 4.01DD11 pKa = 4.13EE12 pKa = 4.71PFALEE17 pKa = 4.66IIADD21 pKa = 4.33NISQVPFLHH30 pKa = 6.87LVGTFSNPYY39 pKa = 9.61DD40 pKa = 3.98ALDD43 pKa = 3.71VLSSGQVDD51 pKa = 3.63ILFLDD56 pKa = 3.37IQMPMLTGVQFLRR69 pKa = 11.84SFPQQTPVILTTAYY83 pKa = 9.45EE84 pKa = 4.28QYY86 pKa = 11.39ALEE89 pKa = 4.22

Molecular weight: 9.95 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|I2GBR3|I2GBR3_9BACT Uncharacterized protein OS=Fibrisoma limi BUZ 3 OX=1185876 GN=BN8_00255 PE=4 SV=1
MM1 pKa = 6.96RR2 pKa = 11.84TIVALLLLCTGCARR16 pKa = 11.84QPTLPADD23 pKa = 3.47SRR25 pKa = 11.84HH26 pKa = 5.54NVVGMLSDD34 pKa = 3.8STWFATGKK42 pKa = 10.38ALRR45 pKa = 11.84LVKK48 pKa = 10.45RR49 pKa = 11.84SEE51 pKa = 4.14APTSVRR57 pKa = 11.84QFNLQITTDD66 pKa = 3.62LGYY69 pKa = 10.06PGNTSANRR77 pKa = 11.84SPIVTGCNGDD87 pKa = 3.74CVPTQRR93 pKa = 11.84LHH95 pKa = 7.29LYY97 pKa = 7.62TIPLRR102 pKa = 11.84KK103 pKa = 9.91GKK105 pKa = 10.41FKK107 pKa = 10.86LSKK110 pKa = 9.2LDD112 pKa = 3.21KK113 pKa = 10.63RR114 pKa = 11.84RR115 pKa = 11.84VVDD118 pKa = 5.29DD119 pKa = 3.64EE120 pKa = 4.27RR121 pKa = 11.84TNYY124 pKa = 8.92WLLINGGGVVKK135 pKa = 10.6NYY137 pKa = 10.04RR138 pKa = 11.84FGGRR142 pKa = 11.84RR143 pKa = 11.84HH144 pKa = 4.51GWVRR148 pKa = 11.84ITRR151 pKa = 11.84YY152 pKa = 9.93DD153 pKa = 3.39RR154 pKa = 11.84QSNVIEE160 pKa = 4.3GRR162 pKa = 11.84FSFDD166 pKa = 3.69LDD168 pKa = 3.71EE169 pKa = 5.0NLNQVGRR176 pKa = 11.84RR177 pKa = 11.84LNSLPSVARR186 pKa = 11.84FRR188 pKa = 11.84DD189 pKa = 3.59GLFRR193 pKa = 11.84IPLEE197 pKa = 4.39DD198 pKa = 3.43VTLKK202 pKa = 10.93DD203 pKa = 3.3

Molecular weight: 23.02 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6341

0

6341

2247638

30

5101

354.5

39.54

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.355 ± 0.03

0.733 ± 0.009

5.339 ± 0.025

5.18 ± 0.029

4.433 ± 0.021

7.234 ± 0.035

1.823 ± 0.016

5.572 ± 0.026

4.534 ± 0.025

10.11 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.059 ± 0.013

4.631 ± 0.027

4.763 ± 0.019

4.626 ± 0.02

5.874 ± 0.026

5.975 ± 0.023

6.512 ± 0.049

7.095 ± 0.023

1.346 ± 0.011

3.805 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski