Candidatus Brocadia sinica JPN1

Taxonomy: cellular organisms; Bacteria; PVC group; Planctomycetes; Candidatus Brocadiae; Candidatus Brocadiales; Candidatus Brocadiaceae; Candidatus Brocadia; Candidatus Brocadia sinica

Average proteome isoelectric point is 7.04

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3878 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0C9PJB9|A0A0C9PJB9_9BACT Cysteine synthase OS=Candidatus Brocadia sinica JPN1 OX=1197129 GN=BROSI_A0303 PE=4 SV=1
MM1 pKa = 7.72NDD3 pKa = 3.69GLCVSAYY10 pKa = 10.65DD11 pKa = 3.87EE12 pKa = 4.31VTITILDD19 pKa = 4.05LNGLPACDD27 pKa = 4.29LAQASPGLIWPPNHH41 pKa = 6.78KK42 pKa = 10.05LVEE45 pKa = 4.1VGITGVTDD53 pKa = 3.84PDD55 pKa = 3.79NNQVTITITGVTQDD69 pKa = 4.13EE70 pKa = 4.53PVDD73 pKa = 3.8GLGDD77 pKa = 4.05GDD79 pKa = 4.11TSPDD83 pKa = 3.6AVIQGDD89 pKa = 4.03KK90 pKa = 10.84VLLRR94 pKa = 11.84AEE96 pKa = 4.13RR97 pKa = 11.84SGNGNGRR104 pKa = 11.84VYY106 pKa = 10.77RR107 pKa = 11.84ITFTADD113 pKa = 2.88DD114 pKa = 4.52GAGGSCTGTVNVCVPHH130 pKa = 6.69SSQSEE135 pKa = 4.46CIDD138 pKa = 4.16DD139 pKa = 4.13GQNYY143 pKa = 10.25NSLQQ147 pKa = 3.37

Molecular weight: 15.29 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0C9PYY7|A0A0C9PYY7_9BACT Ribonuclease Y OS=Candidatus Brocadia sinica JPN1 OX=1197129 GN=rny PE=3 SV=1
MM1 pKa = 7.84PKK3 pKa = 10.57LKK5 pKa = 8.68THH7 pKa = 6.7KK8 pKa = 10.27GLKK11 pKa = 9.64KK12 pKa = 10.16RR13 pKa = 11.84IKK15 pKa = 9.83ISAKK19 pKa = 10.35GKK21 pKa = 8.73VKK23 pKa = 10.4RR24 pKa = 11.84PKK26 pKa = 10.08AGKK29 pKa = 9.6GHH31 pKa = 7.06LLSGKK36 pKa = 8.34SGRR39 pKa = 11.84RR40 pKa = 11.84RR41 pKa = 11.84EE42 pKa = 4.08HH43 pKa = 5.87LRR45 pKa = 11.84KK46 pKa = 8.77KK47 pKa = 9.05TGVSPAFNKK56 pKa = 9.96IMTRR60 pKa = 11.84ALRR63 pKa = 11.84GSS65 pKa = 3.44

Molecular weight: 7.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3878

0

3878

1138923

30

2828

293.7

33.05

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.755 ± 0.036

1.31 ± 0.016

5.235 ± 0.029

6.779 ± 0.041

4.481 ± 0.031

6.986 ± 0.039

2.168 ± 0.02

7.989 ± 0.037

7.333 ± 0.042

9.319 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.421 ± 0.016

4.388 ± 0.031

3.951 ± 0.027

3.164 ± 0.022

5.031 ± 0.036

5.932 ± 0.033

5.466 ± 0.037

6.688 ± 0.028

1.105 ± 0.017

3.497 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski