Balearica regulorum gibbericeps (East African grey crowned-crane)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Sauropsida; Sauria; Archelosauria;

Average proteome isoelectric point is 6.6

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8821 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A087VCA1|A0A087VCA1_BALRE 5-hydroxytryptamine receptor 4 (Fragment) OS=Balearica regulorum gibbericeps OX=100784 GN=N312_02757 PE=3 SV=1
TT1 pKa = 6.72TGDD4 pKa = 3.72VVDD7 pKa = 4.02SCSAAEE13 pKa = 4.06LQLDD17 pKa = 3.99SLYY20 pKa = 10.17LQCMDD25 pKa = 4.32SRR27 pKa = 11.84NADD30 pKa = 3.6VLDD33 pKa = 3.77VLPKK37 pKa = 10.26QEE39 pKa = 4.18IQSSEE44 pKa = 4.06CQAAASDD51 pKa = 4.11AEE53 pKa = 4.38VLHH56 pKa = 6.71RR57 pKa = 11.84ARR59 pKa = 11.84GFLGQYY65 pKa = 5.64TTPKK69 pKa = 10.34VFLSANAPQTDD80 pKa = 3.32ATEE83 pKa = 4.6CEE85 pKa = 4.27VGLADD90 pKa = 4.52TYY92 pKa = 11.44LSPTADD98 pKa = 3.12SCEE101 pKa = 4.25NISLATTDD109 pKa = 4.93KK110 pKa = 11.31GDD112 pKa = 3.78LPHH115 pKa = 7.51SIVYY119 pKa = 10.04QNEE122 pKa = 3.61EE123 pKa = 4.41GKK125 pKa = 9.57WVTDD129 pKa = 3.51LAYY132 pKa = 9.07YY133 pKa = 10.17TSFDD137 pKa = 3.62EE138 pKa = 4.4EE139 pKa = 4.26QNLNLSEE146 pKa = 4.35DD147 pKa = 3.67DD148 pKa = 4.51KK149 pKa = 11.61INEE152 pKa = 4.01EE153 pKa = 4.77FITT156 pKa = 4.04

Molecular weight: 17.1 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A087V2A1|A0A087V2A1_BALRE Uncharacterized protein (Fragment) OS=Balearica regulorum gibbericeps OX=100784 GN=N312_02650 PE=4 SV=1
LL1 pKa = 7.45PARR4 pKa = 11.84QGKK7 pKa = 8.83ARR9 pKa = 11.84QGKK12 pKa = 8.73ARR14 pKa = 11.84QGKK17 pKa = 8.73ARR19 pKa = 11.84QGKK22 pKa = 8.73ARR24 pKa = 11.84QGKK27 pKa = 8.73ARR29 pKa = 11.84QGKK32 pKa = 8.73ARR34 pKa = 11.84QGKK37 pKa = 8.73ARR39 pKa = 11.84QGKK42 pKa = 8.73ARR44 pKa = 11.84QGKK47 pKa = 8.73ARR49 pKa = 11.84QGKK52 pKa = 8.73ARR54 pKa = 11.84QGKK57 pKa = 8.73ARR59 pKa = 11.84QGKK62 pKa = 9.07ARR64 pKa = 3.79

Molecular weight: 6.94 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8821

0

8821

3859431

32

5542

437.5

49.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.335 ± 0.019

2.249 ± 0.021

5.062 ± 0.021

7.195 ± 0.038

3.913 ± 0.021

5.805 ± 0.035

2.522 ± 0.014

5.061 ± 0.022

6.49 ± 0.029

9.68 ± 0.037

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.264 ± 0.01

4.218 ± 0.019

5.227 ± 0.035

4.698 ± 0.025

5.135 ± 0.021

8.234 ± 0.046

5.445 ± 0.018

6.271 ± 0.021

1.174 ± 0.011

3.017 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski