[Candida] haemuloni
Average proteome isoelectric point is 6.25
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5240 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2V1ATU0|A0A2V1ATU0_9ASCO Transcription initiation factor IIF subunit beta OS=[Candida] haemuloni OX=45357 GN=CXQ85_000149 PE=3 SV=1
MM1 pKa = 7.82 KK2 pKa = 10.28 YY3 pKa = 10.21 LALALIASTVAADD16 pKa = 4.1 RR17 pKa = 11.84 YY18 pKa = 10.48 KK19 pKa = 11.02 LYY21 pKa = 11.08 AEE23 pKa = 4.57 TDD25 pKa = 3.25 NEE27 pKa = 4.29 EE28 pKa = 4.31 YY29 pKa = 10.47 NGKK32 pKa = 9.41 GLYY35 pKa = 9.98 ANKK38 pKa = 9.62 EE39 pKa = 3.99 ARR41 pKa = 11.84 SGLDD45 pKa = 3.23 YY46 pKa = 11.54 VFLNDD51 pKa = 3.61 NPIGLEE57 pKa = 3.77 YY58 pKa = 10.87 DD59 pKa = 3.88 EE60 pKa = 5.49 EE61 pKa = 4.24 DD62 pKa = 4.23 HH63 pKa = 7.18 EE64 pKa = 5.3 LEE66 pKa = 4.27 GWDD69 pKa = 3.54 SKK71 pKa = 10.96 MAVLGQYY78 pKa = 11.0 LVMASRR84 pKa = 11.84 GDD86 pKa = 3.44 GDD88 pKa = 4.63 SNVAIADD95 pKa = 3.68 NYY97 pKa = 10.32 LQLSAKK103 pKa = 8.64 TWACKK108 pKa = 8.82 ATQDD112 pKa = 3.55 PNGYY116 pKa = 9.86 DD117 pKa = 3.26 RR118 pKa = 11.84 SNLVYY123 pKa = 10.89 VNEE126 pKa = 4.18 TQPYY130 pKa = 7.92 EE131 pKa = 4.16 DD132 pKa = 3.88 CAEE135 pKa = 3.77 VRR137 pKa = 11.84 FRR139 pKa = 11.84 AEE141 pKa = 3.99 KK142 pKa = 10.74 EE143 pKa = 3.75 GDD145 pKa = 3.37 GDD147 pKa = 4.47 GDD149 pKa = 3.98 GDD151 pKa = 4.55 GDD153 pKa = 4.55 GDD155 pKa = 4.55 GDD157 pKa = 4.55 GDD159 pKa = 4.55 GDD161 pKa = 4.55 GDD163 pKa = 4.55 GDD165 pKa = 4.55 GDD167 pKa = 4.55 GDD169 pKa = 4.55 GDD171 pKa = 4.55 GDD173 pKa = 4.55 GDD175 pKa = 4.55 GDD177 pKa = 4.55 GDD179 pKa = 4.55 GDD181 pKa = 4.46 GDD183 pKa = 5.07 GDD185 pKa = 5.4 GDD187 pKa = 6.07 DD188 pKa = 6.13 DD189 pKa = 7.07 DD190 pKa = 7.68 DD191 pKa = 7.51 DD192 pKa = 6.46 DD193 pKa = 5.27 DD194 pKa = 4.48 HH195 pKa = 6.71 HH196 pKa = 7.05 TPTPSLPTGGWNHH209 pKa = 5.57 TTTTTTLTDD218 pKa = 3.41 YY219 pKa = 7.55 TTYY222 pKa = 10.77 CPTEE226 pKa = 3.9 TTITLTTCGPRR237 pKa = 11.84 HH238 pKa = 5.82 CHH240 pKa = 5.46 PTTITVTEE248 pKa = 4.26 PTTVTVSSCIPPAPTGPEE266 pKa = 3.75 PTGPQPTGPQPTGPEE281 pKa = 4.14 PTVSPPEE288 pKa = 3.98 PTVTSWDD295 pKa = 3.65 GAGTNPVPVLAAFLGFAALLAA316 pKa = 4.74
Molecular weight: 33.24 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.712
IPC2_protein 3.668
IPC_protein 3.706
Toseland 3.478
ProMoST 3.884
Dawson 3.706
Bjellqvist 3.859
Wikipedia 3.668
Rodwell 3.528
Grimsley 3.376
Solomon 3.706
Lehninger 3.668
Nozaki 3.821
DTASelect 4.101
Thurlkill 3.528
EMBOSS 3.668
Sillero 3.834
Patrickios 0.744
IPC_peptide 3.694
IPC2_peptide 3.808
IPC2.peptide.svr19 3.752
Protein with the highest isoelectric point:
>tr|A0A2V1AZE8|A0A2V1AZE8_9ASCO Uncharacterized protein OS=[Candida] haemuloni OX=45357 GN=CXQ85_002923 PE=4 SV=1
MM1 pKa = 7.37 FAGLKK6 pKa = 9.4 HH7 pKa = 5.63 CQRR10 pKa = 11.84 TFRR13 pKa = 11.84 VGINATRR20 pKa = 11.84 QFSMLNKK27 pKa = 9.79 PLVTSSLASPANQASRR43 pKa = 11.84 ISPVAQSLPQTSVLSFLSNLVQKK66 pKa = 10.44 RR67 pKa = 11.84 FKK69 pKa = 11.08 SRR71 pKa = 11.84 GNTYY75 pKa = 10.24 QPSTLKK81 pKa = 10.47 RR82 pKa = 11.84 KK83 pKa = 8.63 RR84 pKa = 11.84 TFGFLARR91 pKa = 11.84 LRR93 pKa = 11.84 TKK95 pKa = 10.52 NGRR98 pKa = 11.84 KK99 pKa = 8.57 VLQRR103 pKa = 11.84 RR104 pKa = 11.84 RR105 pKa = 11.84 AKK107 pKa = 9.93 GRR109 pKa = 11.84 WYY111 pKa = 9.11 LTHH114 pKa = 7.23
Molecular weight: 13.05 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.343
IPC2_protein 10.965
IPC_protein 12.296
Toseland 12.457
ProMoST 12.939
Dawson 12.457
Bjellqvist 12.442
Wikipedia 12.925
Rodwell 12.208
Grimsley 12.501
Solomon 12.939
Lehninger 12.852
Nozaki 12.457
DTASelect 12.442
Thurlkill 12.457
EMBOSS 12.954
Sillero 12.457
Patrickios 11.915
IPC_peptide 12.954
IPC2_peptide 11.93
IPC2.peptide.svr19 9.026
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5240
0
5240
2783537
99
4948
531.2
59.48
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.775 ± 0.03
1.079 ± 0.012
6.037 ± 0.024
6.917 ± 0.039
4.409 ± 0.022
5.665 ± 0.035
2.227 ± 0.014
5.398 ± 0.024
6.813 ± 0.035
9.126 ± 0.04
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.984 ± 0.012
4.778 ± 0.022
4.939 ± 0.035
3.969 ± 0.03
4.587 ± 0.023
9.167 ± 0.045
5.531 ± 0.059
6.284 ± 0.024
1.073 ± 0.011
3.242 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here