Spiroplasma gladiatoris

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Tenericutes; Mollicutes; Entomoplasmatales; Spiroplasmataceae; Spiroplasma

Average proteome isoelectric point is 7.14

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1024 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4P7AI28|A0A4P7AI28_9MOLU 30S ribosomal protein S11 OS=Spiroplasma gladiatoris OX=2143 GN=rpsK PE=3 SV=1
MM1 pKa = 6.41YY2 pKa = 9.25TSLEE6 pKa = 4.16AKK8 pKa = 10.12NLEE11 pKa = 3.79NTYY14 pKa = 10.28ISEE17 pKa = 4.19INFDD21 pKa = 3.92YY22 pKa = 10.79SAKK25 pKa = 9.7GWKK28 pKa = 9.26ISYY31 pKa = 10.95KK32 pKa = 10.97LMIDD36 pKa = 3.63LNGNNTYY43 pKa = 11.0DD44 pKa = 3.75EE45 pKa = 4.41NEE47 pKa = 4.14KK48 pKa = 11.18SNEE51 pKa = 4.11SEE53 pKa = 4.13NSGLEE58 pKa = 4.09YY59 pKa = 10.97

Molecular weight: 6.88 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4P7AIF0|A0A4P7AIF0_9MOLU 50S ribosomal protein L32 OS=Spiroplasma gladiatoris OX=2143 GN=rpmF PE=3 SV=1
MM1 pKa = 7.32KK2 pKa = 9.47RR3 pKa = 11.84TWQPSKK9 pKa = 10.03IKK11 pKa = 10.14HH12 pKa = 5.53ARR14 pKa = 11.84THH16 pKa = 5.78GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.41NGRR28 pKa = 11.84KK29 pKa = 9.2VIKK32 pKa = 10.14ARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.34GRR39 pKa = 11.84AKK41 pKa = 10.69LSAA44 pKa = 3.92

Molecular weight: 5.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1024

0

1024

345192

32

1878

337.1

38.69

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.758 ± 0.083

0.749 ± 0.022

5.474 ± 0.064

6.854 ± 0.082

5.442 ± 0.065

4.82 ± 0.072

1.198 ± 0.032

10.076 ± 0.085

10.525 ± 0.089

9.429 ± 0.073

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.036 ± 0.032

8.057 ± 0.097

2.36 ± 0.036

3.006 ± 0.044

2.656 ± 0.039

6.601 ± 0.068

4.929 ± 0.045

5.721 ± 0.058

0.938 ± 0.025

4.373 ± 0.056
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski