Amino acid dipepetide frequency for Spiroplasma gladiatoris

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
2.651AlaAla: 2.651 ± 0.12
0.507AlaCys: 0.507 ± 0.04
2.031AlaAsp: 2.031 ± 0.095
2.373AlaGlu: 2.373 ± 0.1
2.393AlaPhe: 2.393 ± 0.088
2.538AlaGly: 2.538 ± 0.113
0.626AlaHis: 0.626 ± 0.05
5.368AlaIle: 5.368 ± 0.166
5.287AlaLys: 5.287 ± 0.147
4.719AlaLeu: 4.719 ± 0.145
1.046AlaMet: 1.046 ± 0.055
3.3AlaAsn: 3.3 ± 0.099
1.112AlaPro: 1.112 ± 0.063
1.408AlaGln: 1.408 ± 0.068
1.469AlaArg: 1.469 ± 0.077
3.308AlaSer: 3.308 ± 0.103
2.654AlaThr: 2.654 ± 0.094
2.506AlaVal: 2.506 ± 0.095
0.391AlaTrp: 0.391 ± 0.036
1.892AlaTyr: 1.892 ± 0.081
0.0AlaXaa: 0.0 ± 0.0
Cys
0.385CysAla: 0.385 ± 0.033
0.113CysCys: 0.113 ± 0.022
0.449CysAsp: 0.449 ± 0.037
0.484CysGlu: 0.484 ± 0.037
0.466CysPhe: 0.466 ± 0.038
0.455CysGly: 0.455 ± 0.036
0.156CysHis: 0.156 ± 0.02
0.637CysIle: 0.637 ± 0.045
0.634CysLys: 0.634 ± 0.043
0.857CysLeu: 0.857 ± 0.057
0.133CysMet: 0.133 ± 0.021
0.464CysAsn: 0.464 ± 0.035
0.22CysPro: 0.22 ± 0.027
0.229CysGln: 0.229 ± 0.027
0.133CysArg: 0.133 ± 0.021
0.449CysSer: 0.449 ± 0.043
0.319CysThr: 0.319 ± 0.032
0.458CysVal: 0.458 ± 0.038
0.072CysTrp: 0.072 ± 0.014
0.374CysTyr: 0.374 ± 0.036
0.0CysXaa: 0.0 ± 0.0
Asp
2.213AspAla: 2.213 ± 0.086
0.403AspCys: 0.403 ± 0.036
2.659AspAsp: 2.659 ± 0.115
3.896AspGlu: 3.896 ± 0.13
3.786AspPhe: 3.786 ± 0.111
2.596AspGly: 2.596 ± 0.115
0.626AspHis: 0.626 ± 0.042
5.484AspIle: 5.484 ± 0.13
5.62AspLys: 5.62 ± 0.134
5.82AspLeu: 5.82 ± 0.148
1.005AspMet: 1.005 ± 0.05
3.818AspAsn: 3.818 ± 0.116
1.463AspPro: 1.463 ± 0.077
1.906AspGln: 1.906 ± 0.065
1.173AspArg: 1.173 ± 0.054
3.276AspSer: 3.276 ± 0.124
2.135AspThr: 2.135 ± 0.082
3.056AspVal: 3.056 ± 0.094
0.666AspTrp: 0.666 ± 0.042
3.137AspTyr: 3.137 ± 0.103
0.0AspXaa: 0.0 ± 0.0
Glu
3.294GluAla: 3.294 ± 0.115
0.417GluCys: 0.417 ± 0.037
3.427GluAsp: 3.427 ± 0.115
5.345GluGlu: 5.345 ± 0.165
3.471GluPhe: 3.471 ± 0.115
2.772GluGly: 2.772 ± 0.105
0.846GluHis: 0.846 ± 0.056
7.39GluIle: 7.39 ± 0.153
7.338GluLys: 7.338 ± 0.186
6.727GluLeu: 6.727 ± 0.154
1.504GluMet: 1.504 ± 0.068
5.887GluAsn: 5.887 ± 0.15
1.396GluPro: 1.396 ± 0.064
2.399GluGln: 2.399 ± 0.084
1.79GluArg: 1.79 ± 0.086
3.647GluSer: 3.647 ± 0.103
3.097GluThr: 3.097 ± 0.093
4.351GluVal: 4.351 ± 0.127
0.649GluTrp: 0.649 ± 0.046
2.79GluTyr: 2.79 ± 0.09
0.0GluXaa: 0.0 ± 0.0
Phe
2.683PheAla: 2.683 ± 0.097
0.487PheCys: 0.487 ± 0.041
3.378PheAsp: 3.378 ± 0.102
3.905PheGlu: 3.905 ± 0.11
2.859PhePhe: 2.859 ± 0.115
2.894PheGly: 2.894 ± 0.099
0.446PheHis: 0.446 ± 0.037
5.38PheIle: 5.38 ± 0.163
5.478PheLys: 5.478 ± 0.15
5.423PheLeu: 5.423 ± 0.179
1.049PheMet: 1.049 ± 0.054
4.143PheAsn: 4.143 ± 0.106
0.918PhePro: 0.918 ± 0.052
1.112PheGln: 1.112 ± 0.054
1.243PheArg: 1.243 ± 0.062
4.25PheSer: 4.25 ± 0.129
2.448PheThr: 2.448 ± 0.092
3.276PheVal: 3.276 ± 0.114
0.455PheTrp: 0.455 ± 0.042
2.599PheTyr: 2.599 ± 0.106
0.0PheXaa: 0.0 ± 0.0
Gly
2.677GlyAla: 2.677 ± 0.108
0.388GlyCys: 0.388 ± 0.038
2.529GlyAsp: 2.529 ± 0.116
2.929GlyGlu: 2.929 ± 0.099
2.836GlyPhe: 2.836 ± 0.104
2.856GlyGly: 2.856 ± 0.121
0.736GlyHis: 0.736 ± 0.054
4.542GlyIle: 4.542 ± 0.124
4.096GlyLys: 4.096 ± 0.128
4.528GlyLeu: 4.528 ± 0.122
1.208GlyMet: 1.208 ± 0.064
2.546GlyAsn: 2.546 ± 0.09
1.078GlyPro: 1.078 ± 0.052
1.46GlyGln: 1.46 ± 0.073
1.556GlyArg: 1.556 ± 0.077
3.132GlySer: 3.132 ± 0.107
2.512GlyThr: 2.512 ± 0.092
3.624GlyVal: 3.624 ± 0.121
0.585GlyTrp: 0.585 ± 0.047
2.384GlyTyr: 2.384 ± 0.087
0.0GlyXaa: 0.0 ± 0.0
His
0.582HisAla: 0.582 ± 0.047
0.101HisCys: 0.101 ± 0.016
0.579HisAsp: 0.579 ± 0.049
0.692HisGlu: 0.692 ± 0.047
0.704HisPhe: 0.704 ± 0.046
0.684HisGly: 0.684 ± 0.051
0.229HisHis: 0.229 ± 0.026
1.228HisIle: 1.228 ± 0.069
0.997HisLys: 0.997 ± 0.045
1.22HisLeu: 1.22 ± 0.064
0.203HisMet: 0.203 ± 0.024
0.953HisAsn: 0.953 ± 0.081
0.44HisPro: 0.44 ± 0.035
0.408HisGln: 0.408 ± 0.037
0.417HisArg: 0.417 ± 0.035
0.759HisSer: 0.759 ± 0.05
0.475HisThr: 0.475 ± 0.038
0.649HisVal: 0.649 ± 0.042
0.139HisTrp: 0.139 ± 0.021
0.519HisTyr: 0.519 ± 0.037
0.0HisXaa: 0.0 ± 0.0
Ile
5.345IleAla: 5.345 ± 0.151
0.915IleCys: 0.915 ± 0.054
6.341IleAsp: 6.341 ± 0.127
7.263IleGlu: 7.263 ± 0.165
5.383IlePhe: 5.383 ± 0.154
4.794IleGly: 4.794 ± 0.144
1.078IleHis: 1.078 ± 0.053
9.919IleIle: 9.919 ± 0.23
10.696IleLys: 10.696 ± 0.222
9.047IleLeu: 9.047 ± 0.197
1.909IleMet: 1.909 ± 0.075
8.676IleAsn: 8.676 ± 0.186
2.381IlePro: 2.381 ± 0.097
2.254IleGln: 2.254 ± 0.087
2.549IleArg: 2.549 ± 0.09
6.86IleSer: 6.86 ± 0.134
4.974IleThr: 4.974 ± 0.135
5.678IleVal: 5.678 ± 0.137
0.805IleTrp: 0.805 ± 0.045
3.888IleTyr: 3.888 ± 0.112
0.0IleXaa: 0.0 ± 0.0
Lys
4.551LysAla: 4.551 ± 0.12
0.449LysCys: 0.449 ± 0.04
6.339LysAsp: 6.339 ± 0.119
8.679LysGlu: 8.679 ± 0.165
4.603LysPhe: 4.603 ± 0.112
3.925LysGly: 3.925 ± 0.119
1.286LysHis: 1.286 ± 0.059
10.727LysIle: 10.727 ± 0.203
11.872LysLys: 11.872 ± 0.234
8.572LysLeu: 8.572 ± 0.167
2.236LysMet: 2.236 ± 0.076
10.493LysAsn: 10.493 ± 0.221
2.375LysPro: 2.375 ± 0.094
3.488LysGln: 3.488 ± 0.099
2.813LysArg: 2.813 ± 0.103
5.724LysSer: 5.724 ± 0.119
5.8LysThr: 5.8 ± 0.148
5.733LysVal: 5.733 ± 0.129
0.982LysTrp: 0.982 ± 0.059
4.597LysTyr: 4.597 ± 0.122
0.0LysXaa: 0.0 ± 0.0
Leu
4.725LeuAla: 4.725 ± 0.133
0.733LeuCys: 0.733 ± 0.046
5.154LeuAsp: 5.154 ± 0.121
6.889LeuGlu: 6.889 ± 0.131
4.925LeuPhe: 4.925 ± 0.157
4.424LeuGly: 4.424 ± 0.132
1.002LeuHis: 1.002 ± 0.064
9.56LeuIle: 9.56 ± 0.211
10.719LeuLys: 10.719 ± 0.193
8.219LeuLeu: 8.219 ± 0.184
1.996LeuMet: 1.996 ± 0.085
8.059LeuAsn: 8.059 ± 0.189
2.265LeuPro: 2.265 ± 0.08
2.387LeuGln: 2.387 ± 0.089
2.407LeuArg: 2.407 ± 0.095
6.672LeuSer: 6.672 ± 0.18
4.986LeuThr: 4.986 ± 0.116
5.212LeuVal: 5.212 ± 0.14
0.733LeuTrp: 0.733 ± 0.045
3.224LeuTyr: 3.224 ± 0.099
0.0LeuXaa: 0.0 ± 0.0
Met
1.28MetAla: 1.28 ± 0.07
0.174MetCys: 0.174 ± 0.024
0.857MetAsp: 0.857 ± 0.053
1.112MetGlu: 1.112 ± 0.067
1.121MetPhe: 1.121 ± 0.059
1.243MetGly: 1.243 ± 0.072
0.281MetHis: 0.281 ± 0.031
2.028MetIle: 2.028 ± 0.075
2.132MetLys: 2.132 ± 0.081
1.813MetLeu: 1.813 ± 0.07
0.437MetMet: 0.437 ± 0.042
1.353MetAsn: 1.353 ± 0.069
0.629MetPro: 0.629 ± 0.045
0.776MetGln: 0.776 ± 0.042
0.53MetArg: 0.53 ± 0.042
1.55MetSer: 1.55 ± 0.077
0.956MetThr: 0.956 ± 0.055
1.098MetVal: 1.098 ± 0.062
0.142MetTrp: 0.142 ± 0.018
0.843MetTyr: 0.843 ± 0.055
0.0MetXaa: 0.0 ± 0.0
Asn
3.065AsnAla: 3.065 ± 0.099
0.597AsnCys: 0.597 ± 0.042
4.673AsnAsp: 4.673 ± 0.139
5.238AsnGlu: 5.238 ± 0.148
4.568AsnPhe: 4.568 ± 0.139
3.25AsnGly: 3.25 ± 0.109
0.936AsnHis: 0.936 ± 0.055
7.894AsnIle: 7.894 ± 0.159
9.23AsnLys: 9.23 ± 0.183
7.943AsnLeu: 7.943 ± 0.169
1.443AsnMet: 1.443 ± 0.076
7.448AsnAsn: 7.448 ± 0.204
2.022AsnPro: 2.022 ± 0.071
3.013AsnGln: 3.013 ± 0.106
1.845AsnArg: 1.845 ± 0.073
5.252AsnSer: 5.252 ± 0.144
3.3AsnThr: 3.3 ± 0.1
3.844AsnVal: 3.844 ± 0.108
0.773AsnTrp: 0.773 ± 0.05
4.235AsnTyr: 4.235 ± 0.138
0.0AsnXaa: 0.0 ± 0.0
Pro
1.037ProAla: 1.037 ± 0.063
0.148ProCys: 0.148 ± 0.022
1.185ProAsp: 1.185 ± 0.059
1.698ProGlu: 1.698 ± 0.074
1.382ProPhe: 1.382 ± 0.064
1.393ProGly: 1.393 ± 0.067
0.333ProHis: 0.333 ± 0.03
2.436ProIle: 2.436 ± 0.089
2.158ProLys: 2.158 ± 0.08
2.147ProLeu: 2.147 ± 0.089
0.495ProMet: 0.495 ± 0.037
1.796ProAsn: 1.796 ± 0.08
0.524ProPro: 0.524 ± 0.041
0.672ProGln: 0.672 ± 0.042
0.574ProArg: 0.574 ± 0.045
1.55ProSer: 1.55 ± 0.066
1.385ProThr: 1.385 ± 0.076
1.48ProVal: 1.48 ± 0.071
0.255ProTrp: 0.255 ± 0.032
0.956ProTyr: 0.956 ± 0.054
0.0ProXaa: 0.0 ± 0.0
Gln
1.434GlnAla: 1.434 ± 0.07
0.098GlnCys: 0.098 ± 0.017
1.472GlnAsp: 1.472 ± 0.058
2.222GlnGlu: 2.222 ± 0.078
1.315GlnPhe: 1.315 ± 0.061
1.136GlnGly: 1.136 ± 0.064
0.313GlnHis: 0.313 ± 0.029
3.14GlnIle: 3.14 ± 0.092
3.615GlnLys: 3.615 ± 0.112
2.633GlnLeu: 2.633 ± 0.098
0.695GlnMet: 0.695 ± 0.054
2.842GlnAsn: 2.842 ± 0.105
0.652GlnPro: 0.652 ± 0.047
1.199GlnGln: 1.199 ± 0.083
1.005GlnArg: 1.005 ± 0.056
1.631GlnSer: 1.631 ± 0.069
1.535GlnThr: 1.535 ± 0.074
1.709GlnVal: 1.709 ± 0.073
0.293GlnTrp: 0.293 ± 0.03
1.121GlnTyr: 1.121 ± 0.07
0.0GlnXaa: 0.0 ± 0.0
Arg
1.344ArgAla: 1.344 ± 0.075
0.165ArgCys: 0.165 ± 0.023
1.472ArgAsp: 1.472 ± 0.073
1.935ArgGlu: 1.935 ± 0.08
1.33ArgPhe: 1.33 ± 0.061
1.225ArgGly: 1.225 ± 0.069
0.348ArgHis: 0.348 ± 0.032
2.622ArgIle: 2.622 ± 0.097
2.5ArgLys: 2.5 ± 0.087
2.422ArgLeu: 2.422 ± 0.095
0.666ArgMet: 0.666 ± 0.044
2.01ArgAsn: 2.01 ± 0.082
0.675ArgPro: 0.675 ± 0.046
0.837ArgGln: 0.837 ± 0.058
1.072ArgArg: 1.072 ± 0.063
1.483ArgSer: 1.483 ± 0.071
1.324ArgThr: 1.324 ± 0.069
1.645ArgVal: 1.645 ± 0.075
0.24ArgTrp: 0.24 ± 0.03
1.24ArgTyr: 1.24 ± 0.064
0.0ArgXaa: 0.0 ± 0.0
Ser
2.914SerAla: 2.914 ± 0.1
0.507SerCys: 0.507 ± 0.038
3.32SerAsp: 3.32 ± 0.109
3.827SerGlu: 3.827 ± 0.122
4.015SerPhe: 4.015 ± 0.113
3.424SerGly: 3.424 ± 0.109
0.733SerHis: 0.733 ± 0.049
6.987SerIle: 6.987 ± 0.159
6.753SerLys: 6.753 ± 0.157
6.715SerLeu: 6.715 ± 0.134
1.199SerMet: 1.199 ± 0.063
4.809SerAsn: 4.809 ± 0.138
1.341SerPro: 1.341 ± 0.071
1.976SerGln: 1.976 ± 0.074
1.779SerArg: 1.779 ± 0.078
4.919SerSer: 4.919 ± 0.148
3.537SerThr: 3.537 ± 0.101
3.537SerVal: 3.537 ± 0.112
0.716SerTrp: 0.716 ± 0.054
2.998SerTyr: 2.998 ± 0.12
0.0SerXaa: 0.0 ± 0.0
Thr
2.063ThrAla: 2.063 ± 0.092
0.31ThrCys: 0.31 ± 0.031
2.291ThrAsp: 2.291 ± 0.09
2.202ThrGlu: 2.202 ± 0.084
2.891ThrPhe: 2.891 ± 0.106
2.825ThrGly: 2.825 ± 0.1
0.672ThrHis: 0.672 ± 0.049
5.284ThrIle: 5.284 ± 0.114
4.989ThrLys: 4.989 ± 0.127
4.87ThrLeu: 4.87 ± 0.12
0.924ThrMet: 0.924 ± 0.058
3.975ThrAsn: 3.975 ± 0.119
1.527ThrPro: 1.527 ± 0.075
1.434ThrGln: 1.434 ± 0.072
1.356ThrArg: 1.356 ± 0.066
3.65ThrSer: 3.65 ± 0.091
2.845ThrThr: 2.845 ± 0.106
2.549ThrVal: 2.549 ± 0.09
0.408ThrTrp: 0.408 ± 0.032
2.222ThrTyr: 2.222 ± 0.089
0.0ThrXaa: 0.0 ± 0.0
Val
3.097ValAla: 3.097 ± 0.108
0.55ValCys: 0.55 ± 0.042
3.418ValAsp: 3.418 ± 0.094
4.154ValGlu: 4.154 ± 0.121
3.094ValPhe: 3.094 ± 0.107
3.178ValGly: 3.178 ± 0.119
0.64ValHis: 0.64 ± 0.041
5.527ValIle: 5.527 ± 0.133
5.348ValLys: 5.348 ± 0.132
5.339ValLeu: 5.339 ± 0.133
1.176ValMet: 1.176 ± 0.063
3.899ValAsn: 3.899 ± 0.092
1.44ValPro: 1.44 ± 0.061
1.414ValGln: 1.414 ± 0.074
1.521ValArg: 1.521 ± 0.076
4.012ValSer: 4.012 ± 0.109
2.68ValThr: 2.68 ± 0.095
3.943ValVal: 3.943 ± 0.136
0.487ValTrp: 0.487 ± 0.037
2.297ValTyr: 2.297 ± 0.074
0.0ValXaa: 0.0 ± 0.0
Trp
0.385TrpAla: 0.385 ± 0.038
0.064TrpCys: 0.064 ± 0.013
0.582TrpAsp: 0.582 ± 0.04
0.533TrpGlu: 0.533 ± 0.037
0.591TrpPhe: 0.591 ± 0.045
0.411TrpGly: 0.411 ± 0.037
0.098TrpHis: 0.098 ± 0.016
0.898TrpIle: 0.898 ± 0.059
0.924TrpLys: 0.924 ± 0.055
0.942TrpLeu: 0.942 ± 0.055
0.278TrpMet: 0.278 ± 0.029
0.687TrpAsn: 0.687 ± 0.054
0.162TrpPro: 0.162 ± 0.02
0.272TrpGln: 0.272 ± 0.026
0.168TrpArg: 0.168 ± 0.022
0.663TrpSer: 0.663 ± 0.042
0.501TrpThr: 0.501 ± 0.04
0.629TrpVal: 0.629 ± 0.055
0.156TrpTrp: 0.156 ± 0.02
0.432TrpTyr: 0.432 ± 0.034
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.851TyrAla: 1.851 ± 0.072
0.362TyrCys: 0.362 ± 0.033
2.584TyrAsp: 2.584 ± 0.101
3.161TyrGlu: 3.161 ± 0.115
2.691TyrPhe: 2.691 ± 0.112
2.179TyrGly: 2.179 ± 0.089
0.594TyrHis: 0.594 ± 0.052
3.702TyrIle: 3.702 ± 0.112
4.861TyrLys: 4.861 ± 0.122
4.334TyrLeu: 4.334 ± 0.124
0.75TyrMet: 0.75 ± 0.047
3.413TyrAsn: 3.413 ± 0.113
0.982TyrPro: 0.982 ± 0.053
1.417TyrGln: 1.417 ± 0.081
1.156TyrArg: 1.156 ± 0.063
3.219TyrSer: 3.219 ± 0.103
1.825TyrThr: 1.825 ± 0.067
2.236TyrVal: 2.236 ± 0.092
0.432TyrTrp: 0.432 ± 0.034
1.979TyrTyr: 1.979 ± 0.089
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 1024 proteins (345192 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski