Microbacterium phage Araxxi
Average proteome isoelectric point is 5.77
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 50 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A516KT73|A0A516KT73_9CAUD Uncharacterized protein OS=Microbacterium phage Araxxi OX=2590948 GN=41 PE=4 SV=1
MM1 pKa = 7.85 SDD3 pKa = 3.06 INEE6 pKa = 4.28 NTDD9 pKa = 3.52 GLLTEE14 pKa = 4.69 QDD16 pKa = 3.68 QADD19 pKa = 4.34 LAQLAAEE26 pKa = 4.17 PLEE29 pKa = 4.15 ITVLEE34 pKa = 4.24 TWEE37 pKa = 4.4 SVLSNIEE44 pKa = 3.57 QMEE47 pKa = 4.11 NEE49 pKa = 4.45 KK50 pKa = 10.35 IEE52 pKa = 3.83 PSYY55 pKa = 11.28 AVNIVGKK62 pKa = 9.08 WPKK65 pKa = 10.2 LDD67 pKa = 3.96 YY68 pKa = 11.36 GDD70 pKa = 4.15 VPHH73 pKa = 7.27 FYY75 pKa = 11.23 KK76 pKa = 10.98 LFNEE80 pKa = 3.89 YY81 pKa = 10.25 LKK83 pKa = 10.41 RR84 pKa = 11.84 YY85 pKa = 9.0 RR86 pKa = 11.84 AVLEE90 pKa = 4.1 EE91 pKa = 3.86 QLRR94 pKa = 11.84 LHH96 pKa = 7.31 PDD98 pKa = 2.96 AKK100 pKa = 11.13 DD101 pKa = 3.28 NTGEE105 pKa = 4.12 FGTEE109 pKa = 3.62 EE110 pKa = 3.88 SDD112 pKa = 4.43 AIANRR117 pKa = 11.84 DD118 pKa = 3.18 IYY120 pKa = 11.03 VEE122 pKa = 5.8 LMFGWNVVTAEE133 pKa = 5.4 LEE135 pKa = 4.46 HH136 pKa = 6.67 EE137 pKa = 4.06 WDD139 pKa = 4.14 ALDD142 pKa = 3.33 VDD144 pKa = 4.23 AAEE147 pKa = 5.4 KK148 pKa = 9.5 IAAMGEE154 pKa = 3.95 AQLFVTGPQGMLQALTAPNVGFQWTNEE181 pKa = 3.84 EE182 pKa = 4.11 QIALTEE188 pKa = 4.11 RR189 pKa = 11.84 VVAAAEE195 pKa = 4.04 EE196 pKa = 4.28 LL197 pKa = 3.84
Molecular weight: 22.22 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.245
IPC2_protein 4.062
IPC_protein 3.999
Toseland 3.834
ProMoST 4.113
Dawson 3.948
Bjellqvist 4.101
Wikipedia 3.808
Rodwell 3.834
Grimsley 3.745
Solomon 3.935
Lehninger 3.897
Nozaki 4.062
DTASelect 4.177
Thurlkill 3.846
EMBOSS 3.834
Sillero 4.113
Patrickios 3.516
IPC_peptide 3.948
IPC2_peptide 4.101
IPC2.peptide.svr19 4.017
Protein with the highest isoelectric point:
>tr|A0A516KT34|A0A516KT34_9CAUD Uncharacterized protein OS=Microbacterium phage Araxxi OX=2590948 GN=26 PE=4 SV=1
MM1 pKa = 6.84 NQEE4 pKa = 4.4 LKK6 pKa = 11.08 DD7 pKa = 3.7 PFAGSRR13 pKa = 11.84 LPSDD17 pKa = 3.78 AAAPPQPEE25 pKa = 4.74 RR26 pKa = 11.84 YY27 pKa = 9.42 DD28 pKa = 3.75 QLFLPNDD35 pKa = 3.68 EE36 pKa = 4.65 KK37 pKa = 11.27 PRR39 pKa = 11.84 MPLTKK44 pKa = 9.86 DD45 pKa = 2.8 RR46 pKa = 11.84 YY47 pKa = 8.12 VVRR50 pKa = 11.84 DD51 pKa = 3.83 NPHH54 pKa = 4.81 EE55 pKa = 4.32 VAWEE59 pKa = 4.06 RR60 pKa = 11.84 QVRR63 pKa = 11.84 MYY65 pKa = 11.16 LRR67 pKa = 11.84 GLSLDD72 pKa = 3.71 DD73 pKa = 3.54 SHH75 pKa = 6.38 RR76 pKa = 11.84 TSATMIYY83 pKa = 9.85 EE84 pKa = 4.04 WATGKK89 pKa = 10.28 RR90 pKa = 11.84 VADD93 pKa = 3.89 QIAAGVATSPDD104 pKa = 3.09 LRR106 pKa = 11.84 KK107 pKa = 9.6 INKK110 pKa = 8.92 IMRR113 pKa = 11.84 FYY115 pKa = 10.75 FGKK118 pKa = 9.85 PYY120 pKa = 8.65 QTWIAGRR127 pKa = 11.84 KK128 pKa = 8.74 IPNCYY133 pKa = 9.9 RR134 pKa = 11.84 IPKK137 pKa = 7.65 GWRR140 pKa = 11.84 VKK142 pKa = 8.85 RR143 pKa = 11.84 HH144 pKa = 5.56 RR145 pKa = 11.84 PMTLTLWSEE154 pKa = 4.26 YY155 pKa = 10.79 TEE157 pKa = 4.22 GTLNDD162 pKa = 3.62 DD163 pKa = 3.73 GARR166 pKa = 11.84 RR167 pKa = 11.84 NAAQQ171 pKa = 3.38
Molecular weight: 19.91 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.172
IPC2_protein 9.268
IPC_protein 9.414
Toseland 9.838
ProMoST 9.619
Dawson 10.101
Bjellqvist 9.809
Wikipedia 10.292
Rodwell 10.335
Grimsley 10.189
Solomon 10.131
Lehninger 10.087
Nozaki 9.838
DTASelect 9.794
Thurlkill 9.926
EMBOSS 10.262
Sillero 10.014
Patrickios 7.629
IPC_peptide 10.131
IPC2_peptide 8.478
IPC2.peptide.svr19 8.312
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
50
0
50
16885
43
4494
337.7
37.27
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.104 ± 0.534
0.444 ± 0.166
6.574 ± 0.227
5.988 ± 0.343
3.311 ± 0.141
7.77 ± 0.446
1.617 ± 0.223
4.625 ± 0.184
4.246 ± 0.362
8.22 ± 0.299
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.588 ± 0.168
4.661 ± 0.279
4.904 ± 0.2
4.762 ± 0.199
5.964 ± 0.321
6.189 ± 0.275
6.574 ± 0.338
6.532 ± 0.241
1.836 ± 0.127
3.092 ± 0.179
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here