Amino acid dipepetide frequency for Microbacterium phage Araxxi

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
14.392AlaAla: 14.392 ± 1.32
0.0AlaCys: 0.0 ± 0.0
6.87AlaAsp: 6.87 ± 0.901
6.337AlaGlu: 6.337 ± 0.614
2.606AlaPhe: 2.606 ± 0.404
8.233AlaGly: 8.233 ± 0.618
1.54AlaHis: 1.54 ± 0.353
4.383AlaIle: 4.383 ± 0.559
3.494AlaLys: 3.494 ± 0.453
8.529AlaLeu: 8.529 ± 0.754
2.724AlaMet: 2.724 ± 0.392
4.442AlaAsn: 4.442 ± 0.705
4.857AlaPro: 4.857 ± 0.596
4.679AlaGln: 4.679 ± 0.723
5.982AlaArg: 5.982 ± 0.841
5.745AlaSer: 5.745 ± 0.834
6.397AlaThr: 6.397 ± 0.993
5.745AlaVal: 5.745 ± 0.483
1.836AlaTrp: 1.836 ± 0.244
2.251AlaTyr: 2.251 ± 0.408
0.0AlaXaa: 0.0 ± 0.0
Cys
0.296CysAla: 0.296 ± 0.144
0.0CysCys: 0.0 ± 0.0
0.118CysAsp: 0.118 ± 0.093
0.296CysGlu: 0.296 ± 0.171
0.118CysPhe: 0.118 ± 0.092
0.355CysGly: 0.355 ± 0.199
0.237CysHis: 0.237 ± 0.179
0.178CysIle: 0.178 ± 0.106
0.237CysLys: 0.237 ± 0.145
0.118CysLeu: 0.118 ± 0.087
0.0CysMet: 0.0 ± 0.0
0.237CysAsn: 0.237 ± 0.129
0.355CysPro: 0.355 ± 0.291
0.059CysGln: 0.059 ± 0.056
0.415CysArg: 0.415 ± 0.195
0.355CysSer: 0.355 ± 0.171
0.415CysThr: 0.415 ± 0.247
0.355CysVal: 0.355 ± 0.185
0.059CysTrp: 0.059 ± 0.062
0.237CysTyr: 0.237 ± 0.137
0.0CysXaa: 0.0 ± 0.0
Asp
7.818AspAla: 7.818 ± 0.739
0.415AspCys: 0.415 ± 0.21
3.85AspAsp: 3.85 ± 0.574
4.264AspGlu: 4.264 ± 0.537
2.191AspPhe: 2.191 ± 0.431
5.212AspGly: 5.212 ± 0.574
0.948AspHis: 0.948 ± 0.236
4.027AspIle: 4.027 ± 0.387
2.902AspLys: 2.902 ± 0.328
6.1AspLeu: 6.1 ± 0.91
1.007AspMet: 1.007 ± 0.212
2.724AspAsn: 2.724 ± 0.379
4.561AspPro: 4.561 ± 0.687
2.428AspGln: 2.428 ± 0.257
3.554AspArg: 3.554 ± 0.381
3.317AspSer: 3.317 ± 0.361
3.317AspThr: 3.317 ± 0.382
3.85AspVal: 3.85 ± 0.431
1.244AspTrp: 1.244 ± 0.437
2.014AspTyr: 2.014 ± 0.407
0.0AspXaa: 0.0 ± 0.0
Glu
4.975GluAla: 4.975 ± 0.697
0.296GluCys: 0.296 ± 0.176
3.08GluAsp: 3.08 ± 0.617
3.613GluGlu: 3.613 ± 0.679
2.724GluPhe: 2.724 ± 0.399
4.324GluGly: 4.324 ± 0.613
1.066GluHis: 1.066 ± 0.266
3.258GluIle: 3.258 ± 0.435
3.08GluLys: 3.08 ± 0.493
5.153GluLeu: 5.153 ± 0.514
1.955GluMet: 1.955 ± 0.288
2.547GluAsn: 2.547 ± 0.434
2.369GluPro: 2.369 ± 0.309
2.665GluGln: 2.665 ± 0.45
4.561GluArg: 4.561 ± 0.64
2.902GluSer: 2.902 ± 0.476
3.909GluThr: 3.909 ± 0.519
4.146GluVal: 4.146 ± 0.488
1.421GluTrp: 1.421 ± 0.363
1.836GluTyr: 1.836 ± 0.339
0.0GluXaa: 0.0 ± 0.0
Phe
3.613PheAla: 3.613 ± 0.472
0.118PheCys: 0.118 ± 0.098
3.376PheAsp: 3.376 ± 0.45
2.251PheGlu: 2.251 ± 0.286
1.303PhePhe: 1.303 ± 0.263
2.606PheGly: 2.606 ± 0.358
0.474PheHis: 0.474 ± 0.169
1.244PheIle: 1.244 ± 0.251
1.125PheLys: 1.125 ± 0.175
2.191PheLeu: 2.191 ± 0.287
0.888PheMet: 0.888 ± 0.208
1.777PheAsn: 1.777 ± 0.37
0.948PhePro: 0.948 ± 0.226
1.421PheGln: 1.421 ± 0.333
1.718PheArg: 1.718 ± 0.299
2.132PheSer: 2.132 ± 0.328
2.369PheThr: 2.369 ± 0.373
2.31PheVal: 2.31 ± 0.294
0.237PheTrp: 0.237 ± 0.108
1.007PheTyr: 1.007 ± 0.267
0.0PheXaa: 0.0 ± 0.0
Gly
6.752GlyAla: 6.752 ± 0.856
0.533GlyCys: 0.533 ± 0.253
4.561GlyAsp: 4.561 ± 0.584
4.62GlyGlu: 4.62 ± 0.456
2.073GlyPhe: 2.073 ± 0.287
6.752GlyGly: 6.752 ± 0.939
0.888GlyHis: 0.888 ± 0.247
3.494GlyIle: 3.494 ± 0.498
4.027GlyLys: 4.027 ± 0.789
6.397GlyLeu: 6.397 ± 0.66
2.31GlyMet: 2.31 ± 0.445
4.205GlyAsn: 4.205 ± 0.426
2.606GlyPro: 2.606 ± 0.341
2.961GlyGln: 2.961 ± 0.376
3.672GlyArg: 3.672 ± 0.486
5.271GlySer: 5.271 ± 0.711
5.804GlyThr: 5.804 ± 0.479
5.449GlyVal: 5.449 ± 0.389
2.369GlyTrp: 2.369 ± 0.248
2.961GlyTyr: 2.961 ± 0.275
0.0GlyXaa: 0.0 ± 0.0
His
1.007HisAla: 1.007 ± 0.278
0.118HisCys: 0.118 ± 0.094
1.007HisAsp: 1.007 ± 0.221
1.125HisGlu: 1.125 ± 0.317
0.652HisPhe: 0.652 ± 0.17
1.244HisGly: 1.244 ± 0.345
0.355HisHis: 0.355 ± 0.166
0.474HisIle: 0.474 ± 0.155
0.533HisLys: 0.533 ± 0.162
1.54HisLeu: 1.54 ± 0.47
0.355HisMet: 0.355 ± 0.126
0.415HisAsn: 0.415 ± 0.126
1.244HisPro: 1.244 ± 0.273
1.125HisGln: 1.125 ± 0.283
1.185HisArg: 1.185 ± 0.315
1.125HisSer: 1.125 ± 0.265
0.592HisThr: 0.592 ± 0.188
0.77HisVal: 0.77 ± 0.196
0.296HisTrp: 0.296 ± 0.124
1.007HisTyr: 1.007 ± 0.239
0.0HisXaa: 0.0 ± 0.0
Ile
4.442IleAla: 4.442 ± 0.657
0.118IleCys: 0.118 ± 0.087
3.376IleAsp: 3.376 ± 0.472
3.672IleGlu: 3.672 ± 0.468
0.829IlePhe: 0.829 ± 0.168
3.021IleGly: 3.021 ± 0.36
0.652IleHis: 0.652 ± 0.161
2.073IleIle: 2.073 ± 0.331
2.191IleLys: 2.191 ± 0.468
4.205IleLeu: 4.205 ± 0.637
1.421IleMet: 1.421 ± 0.327
2.191IleAsn: 2.191 ± 0.305
2.547IlePro: 2.547 ± 0.405
2.014IleGln: 2.014 ± 0.41
2.488IleArg: 2.488 ± 0.345
2.843IleSer: 2.843 ± 0.528
3.139IleThr: 3.139 ± 0.475
3.198IleVal: 3.198 ± 0.534
0.77IleTrp: 0.77 ± 0.182
1.066IleTyr: 1.066 ± 0.221
0.0IleXaa: 0.0 ± 0.0
Lys
4.087LysAla: 4.087 ± 0.431
0.178LysCys: 0.178 ± 0.115
2.724LysAsp: 2.724 ± 0.459
1.955LysGlu: 1.955 ± 0.383
0.888LysPhe: 0.888 ± 0.215
3.08LysGly: 3.08 ± 0.691
0.888LysHis: 0.888 ± 0.236
2.191LysIle: 2.191 ± 0.62
2.014LysLys: 2.014 ± 0.548
4.205LysLeu: 4.205 ± 0.545
1.421LysMet: 1.421 ± 0.334
1.777LysAsn: 1.777 ± 0.304
1.955LysPro: 1.955 ± 0.315
1.836LysGln: 1.836 ± 0.313
2.784LysArg: 2.784 ± 0.625
2.665LysSer: 2.665 ± 0.417
2.784LysThr: 2.784 ± 0.471
2.784LysVal: 2.784 ± 0.384
0.829LysTrp: 0.829 ± 0.237
1.421LysTyr: 1.421 ± 0.246
0.0LysXaa: 0.0 ± 0.0
Leu
7.107LeuAla: 7.107 ± 0.584
0.415LeuCys: 0.415 ± 0.207
4.975LeuAsp: 4.975 ± 0.533
5.686LeuGlu: 5.686 ± 0.5
3.494LeuPhe: 3.494 ± 0.386
6.16LeuGly: 6.16 ± 0.649
1.362LeuHis: 1.362 ± 0.304
4.027LeuIle: 4.027 ± 0.43
3.435LeuLys: 3.435 ± 0.373
5.686LeuLeu: 5.686 ± 0.706
2.191LeuMet: 2.191 ± 0.326
4.442LeuAsn: 4.442 ± 0.605
3.85LeuPro: 3.85 ± 0.416
4.738LeuGln: 4.738 ± 0.576
5.627LeuArg: 5.627 ± 0.459
4.738LeuSer: 4.738 ± 0.789
5.864LeuThr: 5.864 ± 0.565
5.034LeuVal: 5.034 ± 0.781
1.125LeuTrp: 1.125 ± 0.29
2.251LeuTyr: 2.251 ± 0.401
0.0LeuXaa: 0.0 ± 0.0
Met
3.494MetAla: 3.494 ± 0.578
0.118MetCys: 0.118 ± 0.097
1.185MetAsp: 1.185 ± 0.216
1.836MetGlu: 1.836 ± 0.301
1.599MetPhe: 1.599 ± 0.296
1.895MetGly: 1.895 ± 0.374
0.296MetHis: 0.296 ± 0.109
0.711MetIle: 0.711 ± 0.196
1.244MetLys: 1.244 ± 0.256
1.895MetLeu: 1.895 ± 0.279
0.711MetMet: 0.711 ± 0.228
0.829MetAsn: 0.829 ± 0.237
1.599MetPro: 1.599 ± 0.247
1.185MetGln: 1.185 ± 0.274
1.244MetArg: 1.244 ± 0.387
2.073MetSer: 2.073 ± 0.377
1.54MetThr: 1.54 ± 0.327
1.125MetVal: 1.125 ± 0.286
0.592MetTrp: 0.592 ± 0.171
0.711MetTyr: 0.711 ± 0.244
0.0MetXaa: 0.0 ± 0.0
Asn
4.324AsnAla: 4.324 ± 0.73
0.415AsnCys: 0.415 ± 0.206
2.606AsnAsp: 2.606 ± 0.376
1.955AsnGlu: 1.955 ± 0.439
1.125AsnPhe: 1.125 ± 0.299
4.146AsnGly: 4.146 ± 0.638
0.948AsnHis: 0.948 ± 0.215
1.836AsnIle: 1.836 ± 0.444
2.073AsnLys: 2.073 ± 0.382
4.442AsnLeu: 4.442 ± 0.658
1.185AsnMet: 1.185 ± 0.271
1.777AsnAsn: 1.777 ± 0.378
2.784AsnPro: 2.784 ± 0.328
1.955AsnGln: 1.955 ± 0.351
2.369AsnArg: 2.369 ± 0.331
3.08AsnSer: 3.08 ± 0.472
3.731AsnThr: 3.731 ± 0.664
3.613AsnVal: 3.613 ± 0.497
0.592AsnTrp: 0.592 ± 0.2
1.658AsnTyr: 1.658 ± 0.422
0.0AsnXaa: 0.0 ± 0.0
Pro
4.975ProAla: 4.975 ± 0.731
0.0ProCys: 0.0 ± 0.0
3.791ProAsp: 3.791 ± 0.464
2.961ProGlu: 2.961 ± 0.42
1.362ProPhe: 1.362 ± 0.28
3.731ProGly: 3.731 ± 0.605
0.829ProHis: 0.829 ± 0.297
2.428ProIle: 2.428 ± 0.45
2.251ProLys: 2.251 ± 0.281
3.968ProLeu: 3.968 ± 0.351
0.888ProMet: 0.888 ± 0.217
2.902ProAsn: 2.902 ± 0.451
2.665ProPro: 2.665 ± 0.544
1.955ProGln: 1.955 ± 0.378
2.191ProArg: 2.191 ± 0.408
2.843ProSer: 2.843 ± 0.344
4.027ProThr: 4.027 ± 0.4
3.376ProVal: 3.376 ± 0.47
0.77ProTrp: 0.77 ± 0.197
1.066ProTyr: 1.066 ± 0.218
0.0ProXaa: 0.0 ± 0.0
Gln
4.679GlnAla: 4.679 ± 0.759
0.237GlnCys: 0.237 ± 0.165
2.073GlnAsp: 2.073 ± 0.288
2.961GlnGlu: 2.961 ± 0.451
1.303GlnPhe: 1.303 ± 0.215
3.376GlnGly: 3.376 ± 0.518
1.185GlnHis: 1.185 ± 0.389
2.073GlnIle: 2.073 ± 0.31
1.836GlnLys: 1.836 ± 0.391
4.146GlnLeu: 4.146 ± 0.372
1.481GlnMet: 1.481 ± 0.372
2.251GlnAsn: 2.251 ± 0.429
2.132GlnPro: 2.132 ± 0.342
3.672GlnGln: 3.672 ± 0.627
3.08GlnArg: 3.08 ± 0.367
2.961GlnSer: 2.961 ± 0.474
2.843GlnThr: 2.843 ± 0.511
3.08GlnVal: 3.08 ± 0.443
0.711GlnTrp: 0.711 ± 0.155
1.54GlnTyr: 1.54 ± 0.238
0.0GlnXaa: 0.0 ± 0.0
Arg
6.16ArgAla: 6.16 ± 0.943
0.415ArgCys: 0.415 ± 0.231
4.324ArgAsp: 4.324 ± 0.375
3.909ArgGlu: 3.909 ± 0.477
2.251ArgPhe: 2.251 ± 0.314
4.324ArgGly: 4.324 ± 0.826
1.007ArgHis: 1.007 ± 0.16
2.31ArgIle: 2.31 ± 0.332
3.08ArgLys: 3.08 ± 0.411
5.212ArgLeu: 5.212 ± 0.586
1.658ArgMet: 1.658 ± 0.325
3.021ArgAsn: 3.021 ± 0.373
2.902ArgPro: 2.902 ± 0.346
2.902ArgGln: 2.902 ± 0.396
3.258ArgArg: 3.258 ± 0.366
2.784ArgSer: 2.784 ± 0.4
3.021ArgThr: 3.021 ± 0.415
3.613ArgVal: 3.613 ± 0.414
1.185ArgTrp: 1.185 ± 0.212
2.31ArgTyr: 2.31 ± 0.401
0.0ArgXaa: 0.0 ± 0.0
Ser
5.33SerAla: 5.33 ± 0.629
0.059SerCys: 0.059 ± 0.077
4.679SerAsp: 4.679 ± 0.583
3.08SerGlu: 3.08 ± 0.4
3.021SerPhe: 3.021 ± 0.46
4.679SerGly: 4.679 ± 0.537
0.77SerHis: 0.77 ± 0.2
3.258SerIle: 3.258 ± 0.442
2.488SerLys: 2.488 ± 0.419
4.975SerLeu: 4.975 ± 0.495
1.303SerMet: 1.303 ± 0.217
2.369SerAsn: 2.369 ± 0.478
2.902SerPro: 2.902 ± 0.452
2.902SerGln: 2.902 ± 0.389
3.791SerArg: 3.791 ± 0.465
4.383SerSer: 4.383 ± 0.627
4.561SerThr: 4.561 ± 0.647
4.027SerVal: 4.027 ± 0.426
1.362SerTrp: 1.362 ± 0.251
1.955SerTyr: 1.955 ± 0.285
0.0SerXaa: 0.0 ± 0.0
Thr
6.633ThrAla: 6.633 ± 0.614
0.237ThrCys: 0.237 ± 0.161
4.442ThrAsp: 4.442 ± 0.56
3.731ThrGlu: 3.731 ± 0.474
2.191ThrPhe: 2.191 ± 0.316
6.1ThrGly: 6.1 ± 0.63
1.066ThrHis: 1.066 ± 0.24
2.369ThrIle: 2.369 ± 0.399
2.251ThrLys: 2.251 ± 0.401
4.857ThrLeu: 4.857 ± 0.487
1.125ThrMet: 1.125 ± 0.198
3.139ThrAsn: 3.139 ± 0.358
3.85ThrPro: 3.85 ± 0.599
2.547ThrGln: 2.547 ± 0.405
3.791ThrArg: 3.791 ± 0.818
4.62ThrSer: 4.62 ± 0.495
4.975ThrThr: 4.975 ± 0.842
4.501ThrVal: 4.501 ± 0.383
1.362ThrTrp: 1.362 ± 0.3
1.955ThrTyr: 1.955 ± 0.441
0.0ThrXaa: 0.0 ± 0.0
Val
6.574ValAla: 6.574 ± 0.76
0.237ValCys: 0.237 ± 0.173
4.738ValAsp: 4.738 ± 0.393
3.317ValGlu: 3.317 ± 0.475
1.895ValPhe: 1.895 ± 0.332
4.916ValGly: 4.916 ± 0.605
0.711ValHis: 0.711 ± 0.186
2.902ValIle: 2.902 ± 0.461
2.31ValLys: 2.31 ± 0.316
4.916ValLeu: 4.916 ± 0.523
1.955ValMet: 1.955 ± 0.378
3.021ValAsn: 3.021 ± 0.376
3.613ValPro: 3.613 ± 0.451
4.146ValGln: 4.146 ± 0.74
4.383ValArg: 4.383 ± 0.414
4.383ValSer: 4.383 ± 0.46
3.968ValThr: 3.968 ± 0.412
4.383ValVal: 4.383 ± 0.478
0.948ValTrp: 0.948 ± 0.323
2.014ValTyr: 2.014 ± 0.286
0.0ValXaa: 0.0 ± 0.0
Trp
2.073TrpAla: 2.073 ± 0.287
0.059TrpCys: 0.059 ± 0.059
1.421TrpAsp: 1.421 ± 0.302
0.592TrpGlu: 0.592 ± 0.237
0.592TrpPhe: 0.592 ± 0.25
1.066TrpGly: 1.066 ± 0.272
0.237TrpHis: 0.237 ± 0.158
1.421TrpIle: 1.421 ± 0.265
0.711TrpLys: 0.711 ± 0.179
1.599TrpLeu: 1.599 ± 0.309
0.415TrpMet: 0.415 ± 0.178
1.066TrpAsn: 1.066 ± 0.24
0.296TrpPro: 0.296 ± 0.133
0.829TrpGln: 0.829 ± 0.15
1.362TrpArg: 1.362 ± 0.318
1.185TrpSer: 1.185 ± 0.375
1.185TrpThr: 1.185 ± 0.257
1.244TrpVal: 1.244 ± 0.287
0.237TrpTrp: 0.237 ± 0.107
0.77TrpTyr: 0.77 ± 0.2
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.31TyrAla: 2.31 ± 0.322
0.474TyrCys: 0.474 ± 0.191
2.547TyrAsp: 2.547 ± 0.373
1.718TyrGlu: 1.718 ± 0.217
0.888TyrPhe: 0.888 ± 0.24
2.488TyrGly: 2.488 ± 0.332
0.711TyrHis: 0.711 ± 0.255
1.599TyrIle: 1.599 ± 0.349
1.185TyrLys: 1.185 ± 0.263
2.073TyrLeu: 2.073 ± 0.243
0.829TyrMet: 0.829 ± 0.26
1.481TyrAsn: 1.481 ± 0.437
1.007TyrPro: 1.007 ± 0.265
1.599TyrGln: 1.599 ± 0.3
2.191TyrArg: 2.191 ± 0.324
2.488TyrSer: 2.488 ± 0.413
1.303TyrThr: 1.303 ± 0.226
2.724TyrVal: 2.724 ± 0.533
0.415TyrTrp: 0.415 ± 0.141
0.888TyrTyr: 0.888 ± 0.3
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 50 proteins (16885 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski