Fulvimarina pelagi HTCC2506
Average proteome isoelectric point is 6.16
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3739 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q0G5I4|Q0G5I4_9RHIZ Proline-rich region:ATP/GTP-binding site motif A (P-loop) OS=Fulvimarina pelagi HTCC2506 OX=314231 GN=FP2506_09561 PE=4 SV=1
MM1 pKa = 7.68 KK2 pKa = 10.59 KK3 pKa = 8.95 LTLSALASVALLAAPALAQDD23 pKa = 4.51 DD24 pKa = 4.07 QTSAEE29 pKa = 4.18 TTAAASEE36 pKa = 4.22 DD37 pKa = 3.94 TAGGDD42 pKa = 3.64 MQPMEE47 pKa = 4.55 VTMNAPDD54 pKa = 3.93 GSDD57 pKa = 2.99 RR58 pKa = 11.84 GTVTVTPTPNGLLLEE73 pKa = 4.67 AEE75 pKa = 4.42 LTGLEE80 pKa = 4.17 PGEE83 pKa = 4.53 HH84 pKa = 6.62 GFHH87 pKa = 6.39 IHH89 pKa = 5.81 EE90 pKa = 4.73 TGQCGEE96 pKa = 4.71 DD97 pKa = 4.16 FSAAGGHH104 pKa = 5.68 YY105 pKa = 10.39 NPTDD109 pKa = 3.52 MEE111 pKa = 4.58 HH112 pKa = 8.29 GYY114 pKa = 7.81 MTEE117 pKa = 4.61 GGPHH121 pKa = 6.83 AGDD124 pKa = 3.56 MPNFTAGEE132 pKa = 4.34 DD133 pKa = 3.88 GSATFAVFNPNLTVSDD149 pKa = 3.47 GDD151 pKa = 4.06 APVMDD156 pKa = 5.94 DD157 pKa = 4.34 DD158 pKa = 5.15 GSAIMVHH165 pKa = 6.45 GGADD169 pKa = 3.61 DD170 pKa = 4.75 YY171 pKa = 11.58 EE172 pKa = 4.39 SQPAGDD178 pKa = 3.72 AGDD181 pKa = 4.07 RR182 pKa = 11.84 VACGVIGGAEE192 pKa = 3.68
Molecular weight: 19.38 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.736
IPC2_protein 3.783
IPC_protein 3.77
Toseland 3.567
ProMoST 3.91
Dawson 3.757
Bjellqvist 3.948
Wikipedia 3.694
Rodwell 3.605
Grimsley 3.478
Solomon 3.757
Lehninger 3.706
Nozaki 3.884
DTASelect 4.101
Thurlkill 3.617
EMBOSS 3.706
Sillero 3.897
Patrickios 1.875
IPC_peptide 3.745
IPC2_peptide 3.872
IPC2.peptide.svr19 3.817
Protein with the highest isoelectric point:
>tr|Q0G1A7|Q0G1A7_9RHIZ Uncharacterized protein OS=Fulvimarina pelagi HTCC2506 OX=314231 GN=FP2506_12944 PE=3 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.43 RR3 pKa = 11.84 TYY5 pKa = 10.27 QPSKK9 pKa = 9.73 LVRR12 pKa = 11.84 KK13 pKa = 8.95 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.63 GFRR19 pKa = 11.84 SRR21 pKa = 11.84 MATVGGRR28 pKa = 11.84 KK29 pKa = 9.29 VIASRR34 pKa = 11.84 RR35 pKa = 11.84 SKK37 pKa = 10.32 GRR39 pKa = 11.84 KK40 pKa = 8.78 RR41 pKa = 11.84 LSAA44 pKa = 3.96
Molecular weight: 5.16 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.433
IPC2_protein 11.125
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.384
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.106
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.067
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3739
0
3739
1100244
20
2839
294.3
32.02
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.667 ± 0.047
0.809 ± 0.013
6.036 ± 0.038
6.649 ± 0.04
3.95 ± 0.032
8.586 ± 0.042
1.881 ± 0.018
5.493 ± 0.029
3.405 ± 0.036
9.601 ± 0.05
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.517 ± 0.02
2.691 ± 0.023
4.876 ± 0.031
2.854 ± 0.027
7.008 ± 0.042
5.888 ± 0.04
5.467 ± 0.027
7.229 ± 0.033
1.196 ± 0.016
2.195 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here