Clostridium cavendishii DSM 21758
Average proteome isoelectric point is 6.58
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4475 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1M6DE85|A0A1M6DE85_9CLOT Ferrous iron transport protein A OS=Clostridium cavendishii DSM 21758 OX=1121302 GN=SAMN02745163_00706 PE=4 SV=1
GG1 pKa = 6.25 ATGVTGATGVTGATGVTGATGITGPLSTGNNAQFITAVSQQLADD45 pKa = 3.43 NDD47 pKa = 3.85 YY48 pKa = 10.23 VEE50 pKa = 5.25 LVNSVINGSNITHH63 pKa = 5.99 VNGTTDD69 pKa = 3.62 IILQGVHH76 pKa = 5.77 TYY78 pKa = 9.43 YY79 pKa = 11.07 VSWEE83 pKa = 3.86 IIAIVSVAASVFDD96 pKa = 6.21 GILEE100 pKa = 4.46 LNDD103 pKa = 3.63 QIISGSLGSSGTIQLIGNEE122 pKa = 4.22 GAASGSAVFVATKK135 pKa = 10.7 VGDD138 pKa = 3.53 NVLRR142 pKa = 11.84 LQYY145 pKa = 7.61 MTSDD149 pKa = 3.22 GGINTITSASVSVIEE164 pKa = 4.85 LAA166 pKa = 4.27
Molecular weight: 16.59 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.76
IPC2_protein 3.884
IPC_protein 3.783
Toseland 3.592
ProMoST 3.973
Dawson 3.783
Bjellqvist 3.948
Wikipedia 3.732
Rodwell 3.617
Grimsley 3.503
Solomon 3.757
Lehninger 3.706
Nozaki 3.923
DTASelect 4.113
Thurlkill 3.668
EMBOSS 3.745
Sillero 3.91
Patrickios 0.693
IPC_peptide 3.757
IPC2_peptide 3.884
IPC2.peptide.svr19 3.809
Protein with the highest isoelectric point:
>tr|A0A1M6TLR2|A0A1M6TLR2_9CLOT Chromosomal replication initiator protein DnaA OS=Clostridium cavendishii DSM 21758 OX=1121302 GN=dnaA PE=3 SV=1
MM1 pKa = 7.14 SRR3 pKa = 11.84 EE4 pKa = 4.04 MKK6 pKa = 10.47 KK7 pKa = 10.24 GGPRR11 pKa = 11.84 VRR13 pKa = 11.84 RR14 pKa = 11.84 SKK16 pKa = 10.87 RR17 pKa = 11.84 KK18 pKa = 8.2 VCAFCVEE25 pKa = 3.91 KK26 pKa = 11.26 AEE28 pKa = 4.82 AIDD31 pKa = 3.83 YY32 pKa = 11.15 KK33 pKa = 11.19 DD34 pKa = 3.31 VTKK37 pKa = 10.63 LRR39 pKa = 11.84 KK40 pKa = 9.42 YY41 pKa = 7.69 VTEE44 pKa = 4.22 RR45 pKa = 11.84 GKK47 pKa = 10.15 ILPRR51 pKa = 11.84 RR52 pKa = 11.84 ISGTCAKK59 pKa = 9.97 HH60 pKa = 5.6 QRR62 pKa = 11.84 QLTDD66 pKa = 4.2 AIKK69 pKa = 10.26 RR70 pKa = 11.84 SRR72 pKa = 11.84 NIALLPFSTDD82 pKa = 2.6
Molecular weight: 9.49 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.319
IPC2_protein 9.706
IPC_protein 10.262
Toseland 10.921
ProMoST 10.526
Dawson 10.965
Bjellqvist 10.643
Wikipedia 11.14
Rodwell 11.316
Grimsley 10.994
Solomon 11.096
Lehninger 11.067
Nozaki 10.891
DTASelect 10.628
Thurlkill 10.891
EMBOSS 11.316
Sillero 10.906
Patrickios 11.052
IPC_peptide 11.111
IPC2_peptide 9.604
IPC2.peptide.svr19 8.665
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4475
0
4475
1407850
39
3646
314.6
35.65
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.277 ± 0.037
1.13 ± 0.016
5.548 ± 0.032
7.393 ± 0.044
4.579 ± 0.026
6.085 ± 0.039
1.254 ± 0.012
10.195 ± 0.051
9.441 ± 0.039
9.089 ± 0.036
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.44 ± 0.02
6.946 ± 0.04
2.629 ± 0.023
2.233 ± 0.017
3.151 ± 0.025
6.324 ± 0.033
4.943 ± 0.034
6.359 ± 0.03
0.705 ± 0.011
4.282 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here