Salsuginibacillus halophilus

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Salsuginibacillus

Average proteome isoelectric point is 5.66

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2921 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2P8HQM1|A0A2P8HQM1_9BACI Scaffold Nfu/NifU family protein OS=Salsuginibacillus halophilus OX=517424 GN=B0H94_104108 PE=4 SV=1
MM1 pKa = 7.33KK2 pKa = 9.97CVKK5 pKa = 10.43GSMVLLFAGVVAGSSQAAAEE25 pKa = 4.15EE26 pKa = 4.57VAFEE30 pKa = 4.22QDD32 pKa = 3.01WKK34 pKa = 10.08TLIDD38 pKa = 4.05DD39 pKa = 4.09RR40 pKa = 11.84NLVEE44 pKa = 5.94DD45 pKa = 4.99ADD47 pKa = 4.19LPGNGEE53 pKa = 4.02IEE55 pKa = 4.23HH56 pKa = 6.49MEE58 pKa = 4.06LFQDD62 pKa = 5.24EE63 pKa = 4.64ILLTDD68 pKa = 4.27GVPFGDD74 pKa = 4.19SFITFVNPEE83 pKa = 4.03NGEE86 pKa = 4.17VIDD89 pKa = 4.63NFTVEE94 pKa = 4.21NPYY97 pKa = 11.29DD98 pKa = 3.85LLIFNQTVDD107 pKa = 3.96DD108 pKa = 4.46YY109 pKa = 11.39IIFDD113 pKa = 4.0GEE115 pKa = 3.72EE116 pKa = 4.18DD117 pKa = 3.59SPYY120 pKa = 10.68DD121 pKa = 3.38YY122 pKa = 11.23TLIYY126 pKa = 9.96SAEE129 pKa = 4.05GDD131 pKa = 3.58KK132 pKa = 10.28VTEE135 pKa = 4.3LKK137 pKa = 10.56DD138 pKa = 3.24AQFIDD143 pKa = 4.8FYY145 pKa = 11.74GEE147 pKa = 3.49NDD149 pKa = 3.39QNILVNGGSGLEE161 pKa = 3.7VRR163 pKa = 11.84DD164 pKa = 4.18FNDD167 pKa = 3.46EE168 pKa = 4.31VIHH171 pKa = 7.21SYY173 pKa = 7.81EE174 pKa = 4.04TEE176 pKa = 3.76ALFEE180 pKa = 4.18FWSTDD185 pKa = 3.14QHH187 pKa = 5.94EE188 pKa = 4.74LNLVLEE194 pKa = 4.76NNMEE198 pKa = 4.1PAIAEE203 pKa = 4.11ADD205 pKa = 3.84NNLKK209 pKa = 9.97PAIIAVDD216 pKa = 3.67NNLEE220 pKa = 4.18QQWEE224 pKa = 4.07ISDD227 pKa = 3.96FPEE230 pKa = 4.78GFADD234 pKa = 3.82EE235 pKa = 5.05PGDD238 pKa = 3.68IRR240 pKa = 11.84SIDD243 pKa = 3.41ASLEE247 pKa = 3.9NTAVIQNRR255 pKa = 11.84EE256 pKa = 4.12NEE258 pKa = 4.27FVTYY262 pKa = 11.15DD263 pKa = 3.18MDD265 pKa = 3.59TGEE268 pKa = 4.3FLTRR272 pKa = 11.84LSDD275 pKa = 3.66YY276 pKa = 11.45SLGNGDD282 pKa = 5.03DD283 pKa = 4.1VVPMIVFDD291 pKa = 4.4DD292 pKa = 5.0RR293 pKa = 11.84IHH295 pKa = 6.69FVDD298 pKa = 4.53EE299 pKa = 4.64SEE301 pKa = 4.18TSNVYY306 pKa = 10.43VYY308 pKa = 10.82DD309 pKa = 3.96EE310 pKa = 3.92NLEE313 pKa = 4.23YY314 pKa = 10.71VDD316 pKa = 3.45HH317 pKa = 7.18HH318 pKa = 5.63EE319 pKa = 4.45TEE321 pKa = 4.1GTEE324 pKa = 3.66IDD326 pKa = 3.68LHH328 pKa = 7.39HH329 pKa = 6.96MYY331 pKa = 9.76MEE333 pKa = 4.25EE334 pKa = 4.39ANYY337 pKa = 10.49QLISDD342 pKa = 4.41YY343 pKa = 11.36EE344 pKa = 4.15NEE346 pKa = 3.99NTKK349 pKa = 10.63LINLSGEE356 pKa = 4.28KK357 pKa = 9.86EE358 pKa = 4.1WTSEE362 pKa = 3.6EE363 pKa = 3.96TLKK366 pKa = 11.1YY367 pKa = 10.85NRR369 pKa = 11.84DD370 pKa = 3.9DD371 pKa = 3.99LSLAGSTLGDD381 pKa = 3.43FAYY384 pKa = 11.26SNFEE388 pKa = 3.87FNNYY392 pKa = 8.39EE393 pKa = 3.86NEE395 pKa = 4.09IYY397 pKa = 11.01LMGVDD402 pKa = 3.61QPMTKK407 pKa = 9.48VHH409 pKa = 6.91EE410 pKa = 4.28IDD412 pKa = 3.0SAYY415 pKa = 10.18FQWTAPMISSDD426 pKa = 3.09GDD428 pKa = 3.51RR429 pKa = 11.84IFFLTNEE436 pKa = 4.17EE437 pKa = 4.8GEE439 pKa = 4.36THH441 pKa = 5.47LTGIEE446 pKa = 4.29RR447 pKa = 11.84STDD450 pKa = 3.26DD451 pKa = 4.9HH452 pKa = 7.42DD453 pKa = 3.84ITPADD458 pKa = 3.93HH459 pKa = 6.89EE460 pKa = 4.56WTIEE464 pKa = 3.9LSQSVDD470 pKa = 3.2DD471 pKa = 4.72HH472 pKa = 7.1SVTEE476 pKa = 3.98SAIYY480 pKa = 10.25IEE482 pKa = 5.18DD483 pKa = 3.58EE484 pKa = 3.93AGEE487 pKa = 4.25RR488 pKa = 11.84IEE490 pKa = 4.61IDD492 pKa = 4.29KK493 pKa = 9.19EE494 pKa = 4.23TADD497 pKa = 4.38QEE499 pKa = 4.87ILIQPADD506 pKa = 3.55GVYY509 pKa = 10.48AAGDD513 pKa = 3.46YY514 pKa = 9.8TLYY517 pKa = 9.83VTDD520 pKa = 4.35EE521 pKa = 4.15LRR523 pKa = 11.84SEE525 pKa = 3.84NGTRR529 pKa = 11.84LNEE532 pKa = 4.18GAAHH536 pKa = 7.04EE537 pKa = 4.53FTVEE541 pKa = 3.61

Molecular weight: 61.46 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2P8H812|A0A2P8H812_9BACI Ornithine aminotransferase OS=Salsuginibacillus halophilus OX=517424 GN=rocD PE=3 SV=1
MM1 pKa = 8.28RR2 pKa = 11.84MMLFWLLVIAGLSAGWKK19 pKa = 6.69MRR21 pKa = 11.84KK22 pKa = 9.22RR23 pKa = 11.84LVPMLLASKK32 pKa = 10.33RR33 pKa = 11.84FRR35 pKa = 11.84KK36 pKa = 10.14YY37 pKa = 10.51GLQLAFSVPGFRR49 pKa = 11.84RR50 pKa = 11.84LAWRR54 pKa = 11.84MII56 pKa = 3.71

Molecular weight: 6.7 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2921

0

2921

855502

29

1530

292.9

32.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.096 ± 0.062

0.622 ± 0.014

5.693 ± 0.059

8.783 ± 0.055

4.137 ± 0.035

7.236 ± 0.042

2.407 ± 0.027

5.867 ± 0.035

4.665 ± 0.049

9.435 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.911 ± 0.025

3.522 ± 0.026

3.95 ± 0.028

4.107 ± 0.038

4.779 ± 0.039

5.474 ± 0.031

5.651 ± 0.032

7.461 ± 0.036

1.057 ± 0.015

3.148 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski