Fulvimarina endophytica
Average proteome isoelectric point is 6.15
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3833 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A371X1R6|A0A371X1R6_9RHIZ Uncharacterized protein OS=Fulvimarina endophytica OX=2293836 GN=DYI37_13440 PE=4 SV=1
MM1 pKa = 7.9 RR2 pKa = 11.84 LNLTASLAAIVLAGAMPAAAQDD24 pKa = 3.58 VYY26 pKa = 11.58 APYY29 pKa = 9.88 EE30 pKa = 4.22 PNPAPPVAAAYY41 pKa = 9.99 DD42 pKa = 3.72 YY43 pKa = 11.61 GDD45 pKa = 3.31 TDD47 pKa = 4.25 FVFEE51 pKa = 4.79 LGIGVAAQPDD61 pKa = 4.19 YY62 pKa = 11.24 LGSDD66 pKa = 3.33 NYY68 pKa = 9.64 MASIYY73 pKa = 10.76 GFGSVEE79 pKa = 3.93 YY80 pKa = 10.93 LNIPGLGAFGGRR92 pKa = 11.84 DD93 pKa = 3.29 GRR95 pKa = 11.84 GFSIGPSFNYY105 pKa = 10.06 VGEE108 pKa = 4.21 RR109 pKa = 11.84 DD110 pKa = 3.97 SNDD113 pKa = 3.07 LAGIDD118 pKa = 4.33 GVDD121 pKa = 3.09 ATYY124 pKa = 11.08 EE125 pKa = 3.88 LGLRR129 pKa = 11.84 AGYY132 pKa = 9.65 EE133 pKa = 3.64 WDD135 pKa = 3.35 NAEE138 pKa = 3.96 VYY140 pKa = 10.34 GALRR144 pKa = 11.84 YY145 pKa = 10.07 GLGGAEE151 pKa = 4.57 GLTGDD156 pKa = 4.41 IGANLIARR164 pKa = 11.84 PTDD167 pKa = 3.41 RR168 pKa = 11.84 LRR170 pKa = 11.84 LKK172 pKa = 10.18 AGPVATFADD181 pKa = 3.7 ADD183 pKa = 3.91 YY184 pKa = 10.89 NDD186 pKa = 4.91 AYY188 pKa = 10.92 FSVSPRR194 pKa = 11.84 EE195 pKa = 4.05 FVNSGGRR202 pKa = 11.84 FAAYY206 pKa = 9.77 DD207 pKa = 3.26 AGGGFNSVGIHH218 pKa = 5.69 GEE220 pKa = 3.44 ARR222 pKa = 11.84 YY223 pKa = 9.76 EE224 pKa = 3.82 IFTDD228 pKa = 3.64 YY229 pKa = 10.62 FVNLDD234 pKa = 3.68 ASWNRR239 pKa = 11.84 LVGDD243 pKa = 4.67 AGDD246 pKa = 3.92 SPIVKK251 pKa = 9.96 VAGDD255 pKa = 3.64 DD256 pKa = 3.48 DD257 pKa = 3.89 QYY259 pKa = 11.69 YY260 pKa = 10.34 IGLGVSKK267 pKa = 10.56 RR268 pKa = 11.84 FSLDD272 pKa = 3.03 LFF274 pKa = 4.45
Molecular weight: 29.01 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.79
IPC2_protein 3.999
IPC_protein 3.999
Toseland 3.783
ProMoST 4.164
Dawson 3.999
Bjellqvist 4.151
Wikipedia 3.948
Rodwell 3.821
Grimsley 3.681
Solomon 3.999
Lehninger 3.948
Nozaki 4.113
DTASelect 4.38
Thurlkill 3.834
EMBOSS 3.948
Sillero 4.126
Patrickios 0.973
IPC_peptide 3.986
IPC2_peptide 4.101
IPC2.peptide.svr19 4.0
Protein with the highest isoelectric point:
>tr|A0A371WYS9|A0A371WYS9_9RHIZ TVP38/TMEM64 family membrane protein OS=Fulvimarina endophytica OX=2293836 GN=DYI37_17905 PE=3 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.43 RR3 pKa = 11.84 TYY5 pKa = 10.27 QPSKK9 pKa = 9.73 LVRR12 pKa = 11.84 KK13 pKa = 8.95 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.63 GFRR19 pKa = 11.84 SRR21 pKa = 11.84 MATVGGRR28 pKa = 11.84 RR29 pKa = 11.84 VIASRR34 pKa = 11.84 RR35 pKa = 11.84 SKK37 pKa = 10.32 GRR39 pKa = 11.84 KK40 pKa = 8.78 RR41 pKa = 11.84 LSAA44 pKa = 3.96
Molecular weight: 5.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.448
IPC2_protein 11.155
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.384
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.106
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.089
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3833
0
3833
1255505
17
6238
327.6
35.45
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.371 ± 0.056
0.78 ± 0.012
6.016 ± 0.038
6.393 ± 0.045
3.809 ± 0.03
8.902 ± 0.049
1.865 ± 0.019
5.233 ± 0.029
2.995 ± 0.032
9.829 ± 0.05
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.419 ± 0.02
2.465 ± 0.024
4.972 ± 0.035
2.772 ± 0.024
7.413 ± 0.049
5.869 ± 0.036
5.358 ± 0.034
7.266 ± 0.033
1.166 ± 0.014
2.106 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here