Arthrobacter phage Ichor

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Amigovirus; unclassified Amigovirus

Average proteome isoelectric point is 6.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 84 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5J6TRG0|A0A5J6TRG0_9CAUD Uncharacterized protein OS=Arthrobacter phage Ichor OX=2599824 GN=42 PE=4 SV=1
MM1 pKa = 7.65TDD3 pKa = 3.07ASNEE7 pKa = 3.89AVEE10 pKa = 5.0AEE12 pKa = 4.29VAEE15 pKa = 4.42APEE18 pKa = 4.36EE19 pKa = 4.1EE20 pKa = 4.76VALQTVDD27 pKa = 4.7NIVSPLRR34 pKa = 11.84YY35 pKa = 8.08TPDD38 pKa = 3.61PCSTCHH44 pKa = 6.55

Molecular weight: 4.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5J6TRG6|A0A5J6TRG6_9CAUD RuvC-like resolvase OS=Arthrobacter phage Ichor OX=2599824 GN=51 PE=4 SV=1
MM1 pKa = 7.62AGVKK5 pKa = 10.31NKK7 pKa = 7.67TAPLKK12 pKa = 10.87VVMKK16 pKa = 10.18QFTGSVSDD24 pKa = 3.79RR25 pKa = 11.84LNTRR29 pKa = 11.84SRR31 pKa = 11.84EE32 pKa = 3.74AAIRR36 pKa = 11.84AGIAAQAAMRR46 pKa = 11.84EE47 pKa = 4.61VIMTTPSDD55 pKa = 3.67INPNKK60 pKa = 9.1PDD62 pKa = 3.75RR63 pKa = 11.84YY64 pKa = 9.52DD65 pKa = 3.24TGNMFNKK72 pKa = 8.16VTHH75 pKa = 5.5STSFRR80 pKa = 11.84HH81 pKa = 5.38QRR83 pKa = 11.84FTVRR87 pKa = 11.84FGWLYY92 pKa = 9.7NHH94 pKa = 6.67KK95 pKa = 10.44QYY97 pKa = 11.18FLTQEE102 pKa = 4.02YY103 pKa = 10.75GGVAFGKK110 pKa = 9.76FQITGMFAMRR120 pKa = 11.84AGLRR124 pKa = 11.84AAEE127 pKa = 3.8RR128 pKa = 11.84VLEE131 pKa = 4.22EE132 pKa = 3.83EE133 pKa = 4.49LKK135 pKa = 11.04AVTRR139 pKa = 11.84KK140 pKa = 9.74KK141 pKa = 10.82

Molecular weight: 15.99 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

84

0

84

17372

24

1569

206.8

22.87

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.913 ± 0.708

0.691 ± 0.096

6.073 ± 0.187

7.144 ± 0.438

3.506 ± 0.126

7.685 ± 0.344

1.831 ± 0.205

5.025 ± 0.196

5.434 ± 0.248

7.765 ± 0.207

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.343 ± 0.119

4.3 ± 0.192

4.168 ± 0.223

3.724 ± 0.18

5.302 ± 0.227

6.027 ± 0.308

6.654 ± 0.314

7.316 ± 0.258

1.75 ± 0.195

3.35 ± 0.159
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski