candidate division MSBL1 archaeon SCGC-AAA382A13

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Euryarchaeota incertae sedis; candidate division MSBL1

Average proteome isoelectric point is 6.33

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 450 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A133VGW7|A0A133VGW7_9EURY KH type-2 domain-containing protein OS=candidate division MSBL1 archaeon SCGC-AAA382A13 OX=1698279 GN=AKJ50_00175 PE=4 SV=1
MM1 pKa = 7.3ISKK4 pKa = 10.31EE5 pKa = 3.68IFRR8 pKa = 11.84DD9 pKa = 3.53EE10 pKa = 4.42KK11 pKa = 10.98GVSVVLGAILILGMLAAVFPIWYY34 pKa = 8.74NYY36 pKa = 9.11YY37 pKa = 10.29VEE39 pKa = 4.47STMKK43 pKa = 10.58SKK45 pKa = 8.82EE46 pKa = 4.34TNHH49 pKa = 6.17MDD51 pKa = 3.62TVRR54 pKa = 11.84DD55 pKa = 3.69QYY57 pKa = 11.69FEE59 pKa = 4.33LQSKK63 pKa = 8.23VSWMDD68 pKa = 3.33GPEE71 pKa = 3.83TGGKK75 pKa = 9.68VKK77 pKa = 10.48IPMNAGSVRR86 pKa = 11.84FFPGQSPAGTLTIDD100 pKa = 3.67PGSDD104 pKa = 2.5INVEE108 pKa = 3.94NTGVQEE114 pKa = 4.9IITVDD119 pKa = 3.26PGSITFVGQNIYY131 pKa = 10.6FPTQTYY137 pKa = 9.58IFEE140 pKa = 4.85GGHH143 pKa = 5.11VFLVQGEE150 pKa = 4.53AEE152 pKa = 4.92LMASPNEE159 pKa = 3.9EE160 pKa = 4.32LILWGDD166 pKa = 3.9SLEE169 pKa = 4.24VKK171 pKa = 10.01YY172 pKa = 10.36PKK174 pKa = 10.53VVGEE178 pKa = 4.28EE179 pKa = 4.07EE180 pKa = 4.92SISSMVTEE188 pKa = 4.53SVTLAWGGRR197 pKa = 11.84NRR199 pKa = 11.84FPEE202 pKa = 3.89NMEE205 pKa = 3.85WKK207 pKa = 9.98NADD210 pKa = 3.57NIRR213 pKa = 11.84INFEE217 pKa = 3.7TRR219 pKa = 11.84YY220 pKa = 8.44EE221 pKa = 4.25NVWVSYY227 pKa = 11.02LEE229 pKa = 4.07EE230 pKa = 3.91KK231 pKa = 10.95AEE233 pKa = 4.3NIEE236 pKa = 3.94NATIFAEE243 pKa = 4.13ADD245 pKa = 3.23NYY247 pKa = 11.0EE248 pKa = 4.17NGAYY252 pKa = 10.2LQIFNDD258 pKa = 3.6NIRR261 pKa = 11.84YY262 pKa = 7.85LVCATEE268 pKa = 3.95TGEE271 pKa = 4.46DD272 pKa = 3.81VEE274 pKa = 4.81VTNPTVEE281 pKa = 4.03TLEE284 pKa = 4.33NGEE287 pKa = 4.14VWADD291 pKa = 3.21AATLRR296 pKa = 11.84MSYY299 pKa = 10.89DD300 pKa = 3.56FNDD303 pKa = 3.34YY304 pKa = 11.07AYY306 pKa = 10.7GEE308 pKa = 4.47TRR310 pKa = 11.84FKK312 pKa = 9.82WRR314 pKa = 11.84KK315 pKa = 9.27EE316 pKa = 3.64NGTWSATGWSSQYY329 pKa = 11.06GADD332 pKa = 3.82DD333 pKa = 4.22NYY335 pKa = 11.5SEE337 pKa = 5.8LITGIRR343 pKa = 11.84RR344 pKa = 11.84NTTYY348 pKa = 10.49EE349 pKa = 3.66YY350 pKa = 10.71KK351 pKa = 10.85AQIKK355 pKa = 10.02YY356 pKa = 10.3DD357 pKa = 3.82SKK359 pKa = 11.37NKK361 pKa = 9.54EE362 pKa = 4.03GKK364 pKa = 8.34TKK366 pKa = 10.1TFYY369 pKa = 10.35TGNKK373 pKa = 8.29EE374 pKa = 4.21SYY376 pKa = 9.83GGDD379 pKa = 3.29GGRR382 pKa = 11.84INPPSAARR390 pKa = 11.84LVGAEE395 pKa = 4.1KK396 pKa = 10.19TSNDD400 pKa = 2.93TMYY403 pKa = 10.1LTFRR407 pKa = 11.84NTGDD411 pKa = 3.41NTIHH415 pKa = 6.77FGDD418 pKa = 4.22SVVAFYY424 pKa = 10.9KK425 pKa = 10.82EE426 pKa = 4.04EE427 pKa = 4.16IQNKK431 pKa = 8.17TYY433 pKa = 10.75EE434 pKa = 4.44DD435 pKa = 3.63WSSTTLGIGPSPIGGEE451 pKa = 3.92WKK453 pKa = 10.77SPTNQISIPEE463 pKa = 4.09NGEE466 pKa = 3.94TGVSISFSPTDD477 pKa = 3.21AKK479 pKa = 10.68FDD481 pKa = 3.37QGEE484 pKa = 4.15FFVYY488 pKa = 10.16KK489 pKa = 10.8AEE491 pKa = 4.33FSNGTQNIYY500 pKa = 10.2FVNVEE505 pKa = 4.13TTATGNTTPNSPSNPSPSDD524 pKa = 3.18GATLSEE530 pKa = 4.5TTSTTLSVNVSDD542 pKa = 5.61PDD544 pKa = 4.0GDD546 pKa = 4.1SMNVSFYY553 pKa = 10.94DD554 pKa = 3.69ASDD557 pKa = 3.65DD558 pKa = 4.72SIIGTDD564 pKa = 3.17SGVIDD569 pKa = 4.66GGTASVTWDD578 pKa = 3.44SLSAGSSYY586 pKa = 11.48DD587 pKa = 3.07WYY589 pKa = 11.11AVADD593 pKa = 4.79DD594 pKa = 4.75GTDD597 pKa = 3.36STTSSTWSFTVNTAPDD613 pKa = 3.84SPTNPSPSDD622 pKa = 3.51GAVLSSGTSSTTLSVDD638 pKa = 3.07VSDD641 pKa = 5.26PDD643 pKa = 4.16GDD645 pKa = 4.25SMDD648 pKa = 3.33VTFYY652 pKa = 10.3TGEE655 pKa = 4.01GTEE658 pKa = 4.24IGTDD662 pKa = 3.25TGVSSGGTASTTYY675 pKa = 10.86NGLSDD680 pKa = 3.7GNTYY684 pKa = 11.02DD685 pKa = 3.41WYY687 pKa = 11.12AEE689 pKa = 4.03ADD691 pKa = 3.88DD692 pKa = 4.33GHH694 pKa = 7.11DD695 pKa = 3.58VTTSSTYY702 pKa = 10.56SFTIGSVTYY711 pKa = 9.93SYY713 pKa = 10.79TYY715 pKa = 10.82VDD717 pKa = 3.76SEE719 pKa = 4.59TIYY722 pKa = 11.05EE723 pKa = 4.32GGTANFSNAQQDD735 pKa = 3.46DD736 pKa = 4.46GNFEE740 pKa = 4.72NIYY743 pKa = 9.28EE744 pKa = 4.28TDD746 pKa = 3.42QNPQPGQGNIDD757 pKa = 3.35YY758 pKa = 10.68RR759 pKa = 11.84EE760 pKa = 4.05NVQHH764 pKa = 6.79NIVNVSGNNPVFQIEE779 pKa = 4.18YY780 pKa = 9.07MISNDD785 pKa = 3.16TGEE788 pKa = 4.33SVDD791 pKa = 3.76IYY793 pKa = 11.29LEE795 pKa = 4.23NFTSGNWDD803 pKa = 3.65HH804 pKa = 7.16IGQLSSISLDD814 pKa = 3.09NFEE817 pKa = 5.4YY818 pKa = 11.13NMPSDD823 pKa = 4.53YY824 pKa = 10.92ISATDD829 pKa = 4.07NIHH832 pKa = 5.29VRR834 pKa = 11.84YY835 pKa = 9.01VQPTDD840 pKa = 3.32DD841 pKa = 3.91SARR844 pKa = 11.84TDD846 pKa = 4.06LLIDD850 pKa = 3.35YY851 pKa = 9.83CRR853 pKa = 11.84VRR855 pKa = 11.84SEE857 pKa = 4.02TT858 pKa = 3.45

Molecular weight: 94.24 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A133VFX7|A0A133VFX7_9EURY Uncharacterized protein OS=candidate division MSBL1 archaeon SCGC-AAA382A13 OX=1698279 GN=AKJ50_01195 PE=4 SV=1
RR1 pKa = 7.89DD2 pKa = 3.49NCDD5 pKa = 3.09LVVDD9 pKa = 3.91VGGIPRR15 pKa = 11.84FYY17 pKa = 9.46PKK19 pKa = 10.38KK20 pKa = 10.66GITLKK25 pKa = 10.87RR26 pKa = 11.84KK27 pKa = 9.82GSWSWTEE34 pKa = 3.48MLMNLIEE41 pKa = 4.93NPQGWLRR48 pKa = 11.84DD49 pKa = 3.39YY50 pKa = 10.57HH51 pKa = 5.96QRR53 pKa = 11.84SNIEE57 pKa = 4.21TIYY60 pKa = 10.0STLKK64 pKa = 10.27RR65 pKa = 11.84DD66 pKa = 3.45FQTRR70 pKa = 11.84PRR72 pKa = 11.84SRR74 pKa = 11.84NLNRR78 pKa = 11.84RR79 pKa = 11.84GG80 pKa = 3.29

Molecular weight: 9.58 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

450

0

450

96092

41

1206

213.5

24.11

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.969 ± 0.131

1.0 ± 0.052

5.749 ± 0.112

9.935 ± 0.158

3.864 ± 0.101

6.94 ± 0.146

1.624 ± 0.048

7.599 ± 0.125

8.473 ± 0.188

9.037 ± 0.139

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.28 ± 0.056

4.241 ± 0.08

3.948 ± 0.061

2.414 ± 0.055

5.052 ± 0.094

6.383 ± 0.106

4.907 ± 0.089

6.457 ± 0.116

1.099 ± 0.045

3.026 ± 0.081
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski