Porcine torque teno virus 2
Average proteome isoelectric point is 7.07
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|G8Z9V8|G8Z9V8_9VIRU Capsid protein OS=Porcine torque teno virus 2 OX=871765 PE=3 SV=1
MM1 pKa = 7.51 EE2 pKa = 4.92 EE3 pKa = 3.49 RR4 pKa = 11.84 WLTVAYY10 pKa = 8.84 CAHH13 pKa = 6.88 GLFCGCKK20 pKa = 9.81 DD21 pKa = 3.68 PKK23 pKa = 10.8 KK24 pKa = 10.09 HH25 pKa = 6.71 LEE27 pKa = 3.92 KK28 pKa = 10.88 CLTDD32 pKa = 5.18 AIADD36 pKa = 3.85 AEE38 pKa = 4.49 GDD40 pKa = 3.39 RR41 pKa = 11.84 HH42 pKa = 6.31 EE43 pKa = 5.77 DD44 pKa = 3.32 GGTGGGDD51 pKa = 2.92 ATFDD55 pKa = 3.25 IGIDD59 pKa = 3.51 ALLAAAAQRR68 pKa = 3.68
Molecular weight: 7.21 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.626
IPC2_protein 4.736
IPC_protein 4.596
Toseland 4.457
ProMoST 4.749
Dawson 4.584
Bjellqvist 4.736
Wikipedia 4.495
Rodwell 4.457
Grimsley 4.368
Solomon 4.584
Lehninger 4.533
Nozaki 4.711
DTASelect 4.914
Thurlkill 4.482
EMBOSS 4.52
Sillero 4.749
Patrickios 3.63
IPC_peptide 4.584
IPC2_peptide 4.736
IPC2.peptide.svr19 4.691
Protein with the highest isoelectric point:
>tr|G8Z9V8|G8Z9V8_9VIRU Capsid protein OS=Porcine torque teno virus 2 OX=871765 PE=3 SV=1
MM1 pKa = 8.01 PYY3 pKa = 9.82 RR4 pKa = 11.84 RR5 pKa = 11.84 YY6 pKa = 9.5 RR7 pKa = 11.84 RR8 pKa = 11.84 RR9 pKa = 11.84 RR10 pKa = 11.84 RR11 pKa = 11.84 RR12 pKa = 11.84 PTRR15 pKa = 11.84 RR16 pKa = 11.84 WRR18 pKa = 11.84 HH19 pKa = 4.47 RR20 pKa = 11.84 RR21 pKa = 11.84 WRR23 pKa = 11.84 RR24 pKa = 11.84 YY25 pKa = 7.81 FRR27 pKa = 11.84 YY28 pKa = 9.49 RR29 pKa = 11.84 YY30 pKa = 9.15 RR31 pKa = 11.84 RR32 pKa = 11.84 APRR35 pKa = 11.84 RR36 pKa = 11.84 RR37 pKa = 11.84 RR38 pKa = 11.84 TKK40 pKa = 8.79 VRR42 pKa = 11.84 RR43 pKa = 11.84 RR44 pKa = 11.84 RR45 pKa = 11.84 RR46 pKa = 11.84 KK47 pKa = 9.72 APVIQWFPPSRR58 pKa = 11.84 RR59 pKa = 11.84 TCLIEE64 pKa = 4.6 GFWPLSHH71 pKa = 6.02 GHH73 pKa = 6.06 WFRR76 pKa = 11.84 TCPPMRR82 pKa = 11.84 RR83 pKa = 11.84 LNGLIFTGGGCDD95 pKa = 2.95 WTQWSLQNLYY105 pKa = 10.4 HH106 pKa = 7.36 EE107 pKa = 4.67 KK108 pKa = 10.89 LNWRR112 pKa = 11.84 NIWTASNVGMEE123 pKa = 4.49 FARR126 pKa = 11.84 FLRR129 pKa = 11.84 GKK131 pKa = 10.07 FYY133 pKa = 10.73 FFRR136 pKa = 11.84 HH137 pKa = 5.6 PWRR140 pKa = 11.84 SYY142 pKa = 10.33 IVTWDD147 pKa = 3.16 QDD149 pKa = 3.94 IPCKK153 pKa = 9.77 PLPYY157 pKa = 10.21 QNLQPLLMLLKK168 pKa = 9.96 KK169 pKa = 8.04 QHH171 pKa = 6.63 KK172 pKa = 9.82 LVLSQKK178 pKa = 10.3 DD179 pKa = 3.66 CNPNRR184 pKa = 11.84 KK185 pKa = 8.65 QKK187 pKa = 10.48 PVTLKK192 pKa = 10.25 FRR194 pKa = 11.84 PPPKK198 pKa = 9.71 LTSQWRR204 pKa = 11.84 LSRR207 pKa = 11.84 EE208 pKa = 3.69 LSKK211 pKa = 10.89 IPLIRR216 pKa = 11.84 LGISLIDD223 pKa = 4.34 LSEE226 pKa = 4.04 PWLEE230 pKa = 3.84 GWGNAFYY237 pKa = 11.12 SVLGYY242 pKa = 9.35 EE243 pKa = 4.41 ASKK246 pKa = 11.09 HH247 pKa = 4.81 SGRR250 pKa = 11.84 WSNWTQMKK258 pKa = 9.28 YY259 pKa = 9.31 FWIYY263 pKa = 9.61 DD264 pKa = 3.69 TGVGNAVYY272 pKa = 10.59 VILLKK277 pKa = 10.49 KK278 pKa = 10.43 DD279 pKa = 3.4 VSDD282 pKa = 4.35 NPGDD286 pKa = 3.61 MATQFVTGSGQHH298 pKa = 6.95 PDD300 pKa = 4.02 AIDD303 pKa = 4.09 HH304 pKa = 6.49 IEE306 pKa = 4.08 MVNEE310 pKa = 4.58 GWPYY314 pKa = 9.43 WLFFYY319 pKa = 10.29 GQSEE323 pKa = 4.1 QGIKK327 pKa = 10.27 KK328 pKa = 9.44 LAHH331 pKa = 6.39 DD332 pKa = 3.59 QDD334 pKa = 3.53 IVRR337 pKa = 11.84 EE338 pKa = 4.03 YY339 pKa = 11.48 ARR341 pKa = 11.84 DD342 pKa = 3.74 PKK344 pKa = 10.9 SKK346 pKa = 10.0 KK347 pKa = 10.37 LKK349 pKa = 10.0 IGVIGWASSNYY360 pKa = 6.33 TTAGSNQNTQSQTPEE375 pKa = 4.64 AIQGGYY381 pKa = 8.0 VAYY384 pKa = 10.03 AGSRR388 pKa = 11.84 IPGAGSITNLFQMGWPGDD406 pKa = 4.08 QNWPPTNQDD415 pKa = 2.76 QTNFNWGLRR424 pKa = 11.84 GLCVLRR430 pKa = 11.84 DD431 pKa = 3.75 NMKK434 pKa = 10.72 LGAQEE439 pKa = 5.52 LDD441 pKa = 4.35 DD442 pKa = 4.54 EE443 pKa = 4.78 CTMLSLFGPFVEE455 pKa = 4.88 KK456 pKa = 11.02 ANTAFATNDD465 pKa = 3.26 PKK467 pKa = 11.25 YY468 pKa = 10.58 FRR470 pKa = 11.84 PEE472 pKa = 3.71 LKK474 pKa = 10.24 DD475 pKa = 3.49 YY476 pKa = 11.59 NVVTKK481 pKa = 10.43 YY482 pKa = 10.82 AFKK485 pKa = 10.32 FQWGGHH491 pKa = 3.76 GTEE494 pKa = 4.41 RR495 pKa = 11.84 FKK497 pKa = 9.27 TTIGDD502 pKa = 3.85 PSTIPCPFEE511 pKa = 4.92 PGEE514 pKa = 4.27 RR515 pKa = 11.84 YY516 pKa = 8.96 HH517 pKa = 8.27 HH518 pKa = 6.43 GVQDD522 pKa = 3.65 PAKK525 pKa = 10.05 VQNTVLNPWDD535 pKa = 4.05 YY536 pKa = 11.7 DD537 pKa = 3.35 CDD539 pKa = 4.96 GIVRR543 pKa = 11.84 TDD545 pKa = 3.21 TLKK548 pKa = 10.96 RR549 pKa = 11.84 LLEE552 pKa = 4.48 LPTEE556 pKa = 4.27 TEE558 pKa = 4.35 EE559 pKa = 4.41 TEE561 pKa = 3.86 KK562 pKa = 10.75 AYY564 pKa = 9.86 PLLGQKK570 pKa = 7.42 TEE572 pKa = 4.39 KK573 pKa = 10.65 EE574 pKa = 4.16 PLSDD578 pKa = 3.53 SDD580 pKa = 3.95 EE581 pKa = 4.33 EE582 pKa = 4.55 SVISSTSSGSSQEE595 pKa = 4.36 EE596 pKa = 3.9 EE597 pKa = 4.05 TQRR600 pKa = 11.84 RR601 pKa = 11.84 RR602 pKa = 11.84 QHH604 pKa = 6.02 KK605 pKa = 8.05 PSKK608 pKa = 10.13 RR609 pKa = 11.84 RR610 pKa = 11.84 LLKK613 pKa = 10.16 HH614 pKa = 5.57 LQRR617 pKa = 11.84 VVKK620 pKa = 10.41 RR621 pKa = 11.84 MKK623 pKa = 9.97 TLL625 pKa = 3.31
Molecular weight: 73.55 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.232
IPC2_protein 9.399
IPC_protein 9.531
Toseland 10.072
ProMoST 9.78
Dawson 10.277
Bjellqvist 9.97
Wikipedia 10.452
Rodwell 10.613
Grimsley 10.35
Solomon 10.306
Lehninger 10.262
Nozaki 10.087
DTASelect 9.955
Thurlkill 10.131
EMBOSS 10.482
Sillero 10.189
Patrickios 10.058
IPC_peptide 10.306
IPC2_peptide 8.741
IPC2.peptide.svr19 8.377
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
879
68
625
293.0
33.87
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.371 ± 3.633
1.934 ± 1.038
5.802 ± 1.854
5.688 ± 0.96
3.641 ± 0.678
7.85 ± 1.494
2.73 ± 0.525
4.323 ± 0.433
6.94 ± 0.645
8.191 ± 0.269
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.706 ± 0.08
3.982 ± 0.986
5.802 ± 1.564
4.778 ± 0.921
8.987 ± 1.328
4.664 ± 1.782
6.143 ± 0.129
3.641 ± 0.932
3.299 ± 0.934
3.527 ± 0.872
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here