Peanut bud necrosis virus
Average proteome isoelectric point is 6.55
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q84405|Q84405_9VIRU Envelopment polyprotein OS=Peanut bud necrosis virus OX=40687 GN=GPp PE=3 SV=1
MM1 pKa = 7.46 KK2 pKa = 10.1 KK3 pKa = 10.47 YY4 pKa = 10.74 YY5 pKa = 10.82 LLVYY9 pKa = 10.52 CLGLVSLFFVSEE21 pKa = 4.57 VYY23 pKa = 10.88 LLNQVDD29 pKa = 3.88 NSVQLRR35 pKa = 11.84 KK36 pKa = 9.74 IQDD39 pKa = 3.33 RR40 pKa = 11.84 YY41 pKa = 10.97 KK42 pKa = 10.9 VDD44 pKa = 3.77 DD45 pKa = 4.53 PEE47 pKa = 5.7 DD48 pKa = 3.56 LVEE51 pKa = 4.57 EE52 pKa = 4.15 EE53 pKa = 5.07 DD54 pKa = 4.41 NIITEE59 pKa = 4.51 IIEE62 pKa = 3.96 PRR64 pKa = 11.84 QKK66 pKa = 10.53 KK67 pKa = 10.03 LSRR70 pKa = 11.84 ILRR73 pKa = 11.84 NDD75 pKa = 2.96 EE76 pKa = 3.75 VTTQSSVSEE85 pKa = 3.91 LSCDD89 pKa = 3.08 IFEE92 pKa = 5.57 KK93 pKa = 9.33 RR94 pKa = 11.84 HH95 pKa = 5.3 CMIKK99 pKa = 10.49 GVSDD103 pKa = 4.51 FNAHH107 pKa = 4.82 YY108 pKa = 10.67 QIDD111 pKa = 3.98 NGNEE115 pKa = 3.88 IISCISNSANIFDD128 pKa = 3.45 ICQYY132 pKa = 10.26 EE133 pKa = 4.33 KK134 pKa = 10.77 EE135 pKa = 4.15 FKK137 pKa = 10.32 KK138 pKa = 10.75 IKK140 pKa = 10.05 FKK142 pKa = 10.97 SFPVVPVLKK151 pKa = 10.85 LEE153 pKa = 4.18 NKK155 pKa = 9.46 KK156 pKa = 10.39 VLEE159 pKa = 4.41 IGTKK163 pKa = 10.07 FFFVDD168 pKa = 4.25 KK169 pKa = 10.82 SNNPINIDD177 pKa = 3.25 PKK179 pKa = 11.31 VNLKK183 pKa = 10.52 SPTVARR189 pKa = 11.84 LSVRR193 pKa = 11.84 LSGDD197 pKa = 3.35 CKK199 pKa = 10.31 INQVSMSSPYY209 pKa = 9.8 QIKK212 pKa = 10.28 LRR214 pKa = 11.84 SEE216 pKa = 4.13 EE217 pKa = 4.15 NIGILIKK224 pKa = 10.48 NVKK227 pKa = 9.43 SSKK230 pKa = 10.13 SGNIKK235 pKa = 10.45 SIAGDD240 pKa = 3.26 TTINFKK246 pKa = 10.84 PEE248 pKa = 4.02 EE249 pKa = 3.96 LDD251 pKa = 3.54 GNHH254 pKa = 6.69 FLLCGDD260 pKa = 4.1 KK261 pKa = 10.89 SSLIAKK267 pKa = 9.67 VDD269 pKa = 3.34 IPVRR273 pKa = 11.84 NCVSKK278 pKa = 11.44 YY279 pKa = 9.76 SDD281 pKa = 3.57 EE282 pKa = 4.08 PKK284 pKa = 10.64 KK285 pKa = 10.85 IFFCTNFSYY294 pKa = 10.01 FKK296 pKa = 9.94 WIFVFLMVAFPISWFIWKK314 pKa = 8.58 TKK316 pKa = 10.1 DD317 pKa = 4.7 ALSIWYY323 pKa = 8.99 DD324 pKa = 3.33 VIGILTYY331 pKa = 10.46 PILWTLNWLWPYY343 pKa = 10.52 FPLKK347 pKa = 10.55 CRR349 pKa = 11.84 ICGCFSFLTHH359 pKa = 6.58 SCTEE363 pKa = 4.01 KK364 pKa = 10.8 CVCNQDD370 pKa = 3.89 KK371 pKa = 10.74 ASKK374 pKa = 10.59 DD375 pKa = 3.57 HH376 pKa = 6.94 TDD378 pKa = 2.88 EE379 pKa = 5.59 CYY381 pKa = 10.88 LFTKK385 pKa = 10.55 DD386 pKa = 3.24 KK387 pKa = 11.15 EE388 pKa = 4.26 EE389 pKa = 3.93 WRR391 pKa = 11.84 KK392 pKa = 8.18 LTLIQQFQFIINTKK406 pKa = 10.16 LSTNFLVFVTKK417 pKa = 10.41 MILASILISYY427 pKa = 9.3 IPSSIALKK435 pKa = 10.35 QPNICVEE442 pKa = 3.72 KK443 pKa = 10.51 CYY445 pKa = 11.27 YY446 pKa = 10.32 NLNLDD451 pKa = 4.1 SLTTDD456 pKa = 2.98 KK457 pKa = 11.33 FGMTDD462 pKa = 3.42 NGYY465 pKa = 7.5 EE466 pKa = 4.01 TCEE469 pKa = 3.93 CSIGNVITEE478 pKa = 4.0 TVYY481 pKa = 10.83 RR482 pKa = 11.84 SGVPMSRR489 pKa = 11.84 ATSLNDD495 pKa = 3.36 CVLGSDD501 pKa = 4.27 LCMVSNNQAQNLFACRR517 pKa = 11.84 NGCNSLSSIKK527 pKa = 10.42 NIPDD531 pKa = 3.27 TKK533 pKa = 10.63 FNKK536 pKa = 9.87 LYY538 pKa = 10.44 KK539 pKa = 9.95 GQSFKK544 pKa = 11.72 GNLTSLKK551 pKa = 9.23 IANRR555 pKa = 11.84 LRR557 pKa = 11.84 EE558 pKa = 4.28 GYY560 pKa = 8.76 MDD562 pKa = 4.73 SPTEE566 pKa = 4.08 SKK568 pKa = 10.49 ILEE571 pKa = 4.18 EE572 pKa = 4.33 EE573 pKa = 4.11 SAKK576 pKa = 10.38 EE577 pKa = 3.71 YY578 pKa = 11.07 KK579 pKa = 9.96 FYY581 pKa = 11.29 KK582 pKa = 9.52 SLKK585 pKa = 9.71 VDD587 pKa = 5.4 DD588 pKa = 5.0 IPPEE592 pKa = 4.0 NLLPRR597 pKa = 11.84 QSLVFSTEE605 pKa = 3.11 VDD607 pKa = 3.09 GKK609 pKa = 10.74 YY610 pKa = 9.97 RR611 pKa = 11.84 YY612 pKa = 9.71 LLEE615 pKa = 4.2 MDD617 pKa = 3.71 IKK619 pKa = 11.17 SNTGSVYY626 pKa = 10.92 LLNDD630 pKa = 4.29 DD631 pKa = 4.2 ATHH634 pKa = 6.64 SPMEE638 pKa = 3.57 FMIYY642 pKa = 9.48 IKK644 pKa = 10.62 SVGVEE649 pKa = 3.31 YY650 pKa = 10.6 DD651 pKa = 3.11 VRR653 pKa = 11.84 YY654 pKa = 9.93 KK655 pKa = 11.16 YY656 pKa = 9.83 STAKK660 pKa = 10.07 IDD662 pKa = 3.86 TTVSDD667 pKa = 4.23 YY668 pKa = 11.57 LVTCTGKK675 pKa = 10.63 CADD678 pKa = 5.18 CIKK681 pKa = 10.37 QKK683 pKa = 10.68 PKK685 pKa = 10.93 VGVLDD690 pKa = 4.06 FCVTPTSWWGCEE702 pKa = 3.82 EE703 pKa = 4.53 LGCLAINEE711 pKa = 4.23 GSICGHH717 pKa = 5.92 CTNIYY722 pKa = 10.33 DD723 pKa = 4.93 LSSLVNIYY731 pKa = 10.07 QVVEE735 pKa = 3.98 SHH737 pKa = 5.44 VTAEE741 pKa = 4.25 VCVKK745 pKa = 10.75 SLDD748 pKa = 3.85 GYY750 pKa = 9.1 SCKK753 pKa = 10.14 KK754 pKa = 10.17 HH755 pKa = 6.52 SDD757 pKa = 3.26 RR758 pKa = 11.84 SPIQTDD764 pKa = 4.38 YY765 pKa = 10.95 YY766 pKa = 10.08 QLDD769 pKa = 3.74 MSIDD773 pKa = 3.46 LHH775 pKa = 7.51 NDD777 pKa = 2.92 YY778 pKa = 10.78 MSTDD782 pKa = 3.11 KK783 pKa = 11.27 LFAVTKK789 pKa = 7.15 QQKK792 pKa = 9.81 ILTGNIADD800 pKa = 4.66 LGDD803 pKa = 3.62 FAGSSFGHH811 pKa = 5.51 PQITIDD817 pKa = 5.04 GIPLSVPATLSQNDD831 pKa = 4.03 FTWSCSAVGDD841 pKa = 3.62 KK842 pKa = 10.46 KK843 pKa = 11.66 VNIKK847 pKa = 10.15 QCGLYY852 pKa = 9.02 TYY854 pKa = 10.59 SAIYY858 pKa = 9.9 VLSPSKK864 pKa = 10.66 DD865 pKa = 3.43 VSHH868 pKa = 7.56 LDD870 pKa = 3.45 EE871 pKa = 5.03 NSNKK875 pKa = 10.45 LYY877 pKa = 9.75 MEE879 pKa = 4.61 KK880 pKa = 10.79 DD881 pKa = 3.46 FLVGKK886 pKa = 10.29 LKK888 pKa = 10.36 MVVDD892 pKa = 4.18 MPKK895 pKa = 10.84 EE896 pKa = 3.97 MFKK899 pKa = 10.71 KK900 pKa = 10.41 IPTKK904 pKa = 10.41 PIISEE909 pKa = 3.83 AKK911 pKa = 8.95 MICSGCSQCAVGIDD925 pKa = 3.78 CNITYY930 pKa = 9.74 TSDD933 pKa = 3.11 TTFSARR939 pKa = 11.84 LMMDD943 pKa = 3.59 TCSFKK948 pKa = 10.77 SDD950 pKa = 3.37 QLGTFLGPNEE960 pKa = 4.33 KK961 pKa = 10.24 SIKK964 pKa = 9.84 AYY966 pKa = 10.24 CSEE969 pKa = 4.41 EE970 pKa = 3.89 ITDD973 pKa = 4.37 KK974 pKa = 10.92 SLKK977 pKa = 10.29 LIPEE981 pKa = 4.46 DD982 pKa = 4.01 QEE984 pKa = 4.11 EE985 pKa = 4.64 LTVDD989 pKa = 3.28 IQVDD993 pKa = 3.75 EE994 pKa = 4.65 FTQVDD999 pKa = 3.9 QDD1001 pKa = 4.34 TIIHH1005 pKa = 6.59 FDD1007 pKa = 3.87 DD1008 pKa = 5.37 KK1009 pKa = 11.3 SAHH1012 pKa = 6.98 DD1013 pKa = 4.17 EE1014 pKa = 4.3 NKK1016 pKa = 9.65 HH1017 pKa = 6.34 HH1018 pKa = 7.3 SDD1020 pKa = 3.33 TSISSLWDD1028 pKa = 3.48 WIKK1031 pKa = 11.43 APFNWIASFFGSFFDD1046 pKa = 3.64 LVRR1049 pKa = 11.84 IILVIVAACVGLYY1062 pKa = 9.71 IVSSIFKK1069 pKa = 10.52 LSRR1072 pKa = 11.84 TYY1074 pKa = 11.0 YY1075 pKa = 8.94 VDD1077 pKa = 3.07 KK1078 pKa = 10.72 RR1079 pKa = 11.84 RR1080 pKa = 11.84 QKK1082 pKa = 11.05 LEE1084 pKa = 3.82 DD1085 pKa = 4.69 AIEE1088 pKa = 4.28 SVEE1091 pKa = 4.65 SSVLLANYY1099 pKa = 8.84 TGIDD1103 pKa = 3.25 QTRR1106 pKa = 11.84 KK1107 pKa = 9.77 RR1108 pKa = 11.84 KK1109 pKa = 9.82 SPPKK1113 pKa = 10.42 GYY1115 pKa = 10.48 DD1116 pKa = 3.1 FSLDD1120 pKa = 3.12 II1121 pKa = 5.22
Molecular weight: 127.34 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.476
IPC2_protein 5.626
IPC_protein 5.639
Toseland 5.753
ProMoST 5.944
Dawson 5.83
Bjellqvist 5.881
Wikipedia 5.804
Rodwell 5.792
Grimsley 5.779
Solomon 5.83
Lehninger 5.817
Nozaki 6.059
DTASelect 6.249
Thurlkill 6.173
EMBOSS 6.135
Sillero 6.148
Patrickios 3.77
IPC_peptide 5.842
IPC2_peptide 6.109
IPC2.peptide.svr19 6.215
Protein with the highest isoelectric point:
>tr|Q84404|Q84404_9VIRU Non-structural protein OS=Peanut bud necrosis virus OX=40687 GN=NSm PE=4 SV=1
MM1 pKa = 7.52 SNVKK5 pKa = 9.98 QLTEE9 pKa = 4.08 KK10 pKa = 10.51 KK11 pKa = 10.05 IKK13 pKa = 9.93 EE14 pKa = 4.02 LLAGGSADD22 pKa = 4.03 VEE24 pKa = 4.45 IEE26 pKa = 4.46 TEE28 pKa = 4.09 DD29 pKa = 3.57 STPGFSFKK37 pKa = 10.96 AFYY40 pKa = 8.24 DD41 pKa = 3.67 TNKK44 pKa = 10.16 NLEE47 pKa = 4.03 ITFTNCLNILKK58 pKa = 9.92 CRR60 pKa = 11.84 KK61 pKa = 9.63 QIFAACKK68 pKa = 7.45 SGKK71 pKa = 9.77 YY72 pKa = 7.99 VFCGKK77 pKa = 9.11 TIVATNTDD85 pKa = 3.75 VGPDD89 pKa = 2.87 DD90 pKa = 3.44 WTFKK94 pKa = 9.6 RR95 pKa = 11.84 TEE97 pKa = 3.6 AFIRR101 pKa = 11.84 TKK103 pKa = 9.04 MASMVEE109 pKa = 3.99 KK110 pKa = 10.61 SKK112 pKa = 11.51 NDD114 pKa = 3.46 AAKK117 pKa = 10.37 QEE119 pKa = 4.19 MYY121 pKa = 10.86 NKK123 pKa = 9.67 IMEE126 pKa = 4.76 LPLVAAYY133 pKa = 9.82 GLNVPASFDD142 pKa = 3.51 TCALRR147 pKa = 11.84 MMLCIGGPLPLLSSMTGLAPIIFPLAYY174 pKa = 8.37 YY175 pKa = 10.6 QNVKK179 pKa = 10.31 KK180 pKa = 10.66 EE181 pKa = 3.8 KK182 pKa = 10.6 LGIKK186 pKa = 10.06 NFSTYY191 pKa = 9.83 EE192 pKa = 3.92 QVCKK196 pKa = 9.96 VAKK199 pKa = 10.09 VLSASQIEE207 pKa = 4.69 FKK209 pKa = 11.32 NEE211 pKa = 3.41 LEE213 pKa = 4.25 EE214 pKa = 4.18 MFKK217 pKa = 10.96 SAVKK221 pKa = 10.36 LLSEE225 pKa = 4.79 SNPGTASSISLKK237 pKa = 10.73 KK238 pKa = 10.13 YY239 pKa = 10.29 DD240 pKa = 3.9 EE241 pKa = 4.12 QVKK244 pKa = 10.65 YY245 pKa = 9.75 MDD247 pKa = 3.99 KK248 pKa = 11.02 AFSASLSMDD257 pKa = 3.49 DD258 pKa = 4.54 YY259 pKa = 11.95 GEE261 pKa = 4.16 HH262 pKa = 6.2 SKK264 pKa = 10.97 KK265 pKa = 10.23 KK266 pKa = 9.73 SSKK269 pKa = 10.33 AGPSLEE275 pKa = 4.06 LL276 pKa = 4.47
Molecular weight: 30.61 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.576
IPC2_protein 7.907
IPC_protein 7.761
Toseland 7.585
ProMoST 8.331
Dawson 8.58
Bjellqvist 8.77
Wikipedia 8.536
Rodwell 8.653
Grimsley 7.454
Solomon 8.697
Lehninger 8.697
Nozaki 9.004
DTASelect 8.551
Thurlkill 8.668
EMBOSS 8.785
Sillero 8.931
Patrickios 4.507
IPC_peptide 8.697
IPC2_peptide 7.454
IPC2.peptide.svr19 7.827
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
5020
276
2877
1004.0
114.54
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.263 ± 0.499
2.271 ± 0.484
5.876 ± 0.423
6.394 ± 0.338
4.781 ± 0.254
4.084 ± 0.321
1.813 ± 0.247
8.227 ± 0.312
9.104 ± 0.395
9.163 ± 0.378
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.307 ± 0.551
6.315 ± 0.499
2.988 ± 0.277
2.968 ± 0.071
3.127 ± 0.28
9.542 ± 0.347
5.339 ± 0.211
5.598 ± 0.545
0.797 ± 0.174
4.044 ± 0.316
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here