Peanut bud necrosis virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina; Ellioviricetes; Bunyavirales; Tospoviridae; Orthotospovirus; Groundnut bud necrosis orthotospovirus

Average proteome isoelectric point is 6.55

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|Q84405|Q84405_9VIRU Envelopment polyprotein OS=Peanut bud necrosis virus OX=40687 GN=GPp PE=3 SV=1
MM1 pKa = 7.46KK2 pKa = 10.1KK3 pKa = 10.47YY4 pKa = 10.74YY5 pKa = 10.82LLVYY9 pKa = 10.52CLGLVSLFFVSEE21 pKa = 4.57VYY23 pKa = 10.88LLNQVDD29 pKa = 3.88NSVQLRR35 pKa = 11.84KK36 pKa = 9.74IQDD39 pKa = 3.33RR40 pKa = 11.84YY41 pKa = 10.97KK42 pKa = 10.9VDD44 pKa = 3.77DD45 pKa = 4.53PEE47 pKa = 5.7DD48 pKa = 3.56LVEE51 pKa = 4.57EE52 pKa = 4.15EE53 pKa = 5.07DD54 pKa = 4.41NIITEE59 pKa = 4.51IIEE62 pKa = 3.96PRR64 pKa = 11.84QKK66 pKa = 10.53KK67 pKa = 10.03LSRR70 pKa = 11.84ILRR73 pKa = 11.84NDD75 pKa = 2.96EE76 pKa = 3.75VTTQSSVSEE85 pKa = 3.91LSCDD89 pKa = 3.08IFEE92 pKa = 5.57KK93 pKa = 9.33RR94 pKa = 11.84HH95 pKa = 5.3CMIKK99 pKa = 10.49GVSDD103 pKa = 4.51FNAHH107 pKa = 4.82YY108 pKa = 10.67QIDD111 pKa = 3.98NGNEE115 pKa = 3.88IISCISNSANIFDD128 pKa = 3.45ICQYY132 pKa = 10.26EE133 pKa = 4.33KK134 pKa = 10.77EE135 pKa = 4.15FKK137 pKa = 10.32KK138 pKa = 10.75IKK140 pKa = 10.05FKK142 pKa = 10.97SFPVVPVLKK151 pKa = 10.85LEE153 pKa = 4.18NKK155 pKa = 9.46KK156 pKa = 10.39VLEE159 pKa = 4.41IGTKK163 pKa = 10.07FFFVDD168 pKa = 4.25KK169 pKa = 10.82SNNPINIDD177 pKa = 3.25PKK179 pKa = 11.31VNLKK183 pKa = 10.52SPTVARR189 pKa = 11.84LSVRR193 pKa = 11.84LSGDD197 pKa = 3.35CKK199 pKa = 10.31INQVSMSSPYY209 pKa = 9.8QIKK212 pKa = 10.28LRR214 pKa = 11.84SEE216 pKa = 4.13EE217 pKa = 4.15NIGILIKK224 pKa = 10.48NVKK227 pKa = 9.43SSKK230 pKa = 10.13SGNIKK235 pKa = 10.45SIAGDD240 pKa = 3.26TTINFKK246 pKa = 10.84PEE248 pKa = 4.02EE249 pKa = 3.96LDD251 pKa = 3.54GNHH254 pKa = 6.69FLLCGDD260 pKa = 4.1KK261 pKa = 10.89SSLIAKK267 pKa = 9.67VDD269 pKa = 3.34IPVRR273 pKa = 11.84NCVSKK278 pKa = 11.44YY279 pKa = 9.76SDD281 pKa = 3.57EE282 pKa = 4.08PKK284 pKa = 10.64KK285 pKa = 10.85IFFCTNFSYY294 pKa = 10.01FKK296 pKa = 9.94WIFVFLMVAFPISWFIWKK314 pKa = 8.58TKK316 pKa = 10.1DD317 pKa = 4.7ALSIWYY323 pKa = 8.99DD324 pKa = 3.33VIGILTYY331 pKa = 10.46PILWTLNWLWPYY343 pKa = 10.52FPLKK347 pKa = 10.55CRR349 pKa = 11.84ICGCFSFLTHH359 pKa = 6.58SCTEE363 pKa = 4.01KK364 pKa = 10.8CVCNQDD370 pKa = 3.89KK371 pKa = 10.74ASKK374 pKa = 10.59DD375 pKa = 3.57HH376 pKa = 6.94TDD378 pKa = 2.88EE379 pKa = 5.59CYY381 pKa = 10.88LFTKK385 pKa = 10.55DD386 pKa = 3.24KK387 pKa = 11.15EE388 pKa = 4.26EE389 pKa = 3.93WRR391 pKa = 11.84KK392 pKa = 8.18LTLIQQFQFIINTKK406 pKa = 10.16LSTNFLVFVTKK417 pKa = 10.41MILASILISYY427 pKa = 9.3IPSSIALKK435 pKa = 10.35QPNICVEE442 pKa = 3.72KK443 pKa = 10.51CYY445 pKa = 11.27YY446 pKa = 10.32NLNLDD451 pKa = 4.1SLTTDD456 pKa = 2.98KK457 pKa = 11.33FGMTDD462 pKa = 3.42NGYY465 pKa = 7.5EE466 pKa = 4.01TCEE469 pKa = 3.93CSIGNVITEE478 pKa = 4.0TVYY481 pKa = 10.83RR482 pKa = 11.84SGVPMSRR489 pKa = 11.84ATSLNDD495 pKa = 3.36CVLGSDD501 pKa = 4.27LCMVSNNQAQNLFACRR517 pKa = 11.84NGCNSLSSIKK527 pKa = 10.42NIPDD531 pKa = 3.27TKK533 pKa = 10.63FNKK536 pKa = 9.87LYY538 pKa = 10.44KK539 pKa = 9.95GQSFKK544 pKa = 11.72GNLTSLKK551 pKa = 9.23IANRR555 pKa = 11.84LRR557 pKa = 11.84EE558 pKa = 4.28GYY560 pKa = 8.76MDD562 pKa = 4.73SPTEE566 pKa = 4.08SKK568 pKa = 10.49ILEE571 pKa = 4.18EE572 pKa = 4.33EE573 pKa = 4.11SAKK576 pKa = 10.38EE577 pKa = 3.71YY578 pKa = 11.07KK579 pKa = 9.96FYY581 pKa = 11.29KK582 pKa = 9.52SLKK585 pKa = 9.71VDD587 pKa = 5.4DD588 pKa = 5.0IPPEE592 pKa = 4.0NLLPRR597 pKa = 11.84QSLVFSTEE605 pKa = 3.11VDD607 pKa = 3.09GKK609 pKa = 10.74YY610 pKa = 9.97RR611 pKa = 11.84YY612 pKa = 9.71LLEE615 pKa = 4.2MDD617 pKa = 3.71IKK619 pKa = 11.17SNTGSVYY626 pKa = 10.92LLNDD630 pKa = 4.29DD631 pKa = 4.2ATHH634 pKa = 6.64SPMEE638 pKa = 3.57FMIYY642 pKa = 9.48IKK644 pKa = 10.62SVGVEE649 pKa = 3.31YY650 pKa = 10.6DD651 pKa = 3.11VRR653 pKa = 11.84YY654 pKa = 9.93KK655 pKa = 11.16YY656 pKa = 9.83STAKK660 pKa = 10.07IDD662 pKa = 3.86TTVSDD667 pKa = 4.23YY668 pKa = 11.57LVTCTGKK675 pKa = 10.63CADD678 pKa = 5.18CIKK681 pKa = 10.37QKK683 pKa = 10.68PKK685 pKa = 10.93VGVLDD690 pKa = 4.06FCVTPTSWWGCEE702 pKa = 3.82EE703 pKa = 4.53LGCLAINEE711 pKa = 4.23GSICGHH717 pKa = 5.92CTNIYY722 pKa = 10.33DD723 pKa = 4.93LSSLVNIYY731 pKa = 10.07QVVEE735 pKa = 3.98SHH737 pKa = 5.44VTAEE741 pKa = 4.25VCVKK745 pKa = 10.75SLDD748 pKa = 3.85GYY750 pKa = 9.1SCKK753 pKa = 10.14KK754 pKa = 10.17HH755 pKa = 6.52SDD757 pKa = 3.26RR758 pKa = 11.84SPIQTDD764 pKa = 4.38YY765 pKa = 10.95YY766 pKa = 10.08QLDD769 pKa = 3.74MSIDD773 pKa = 3.46LHH775 pKa = 7.51NDD777 pKa = 2.92YY778 pKa = 10.78MSTDD782 pKa = 3.11KK783 pKa = 11.27LFAVTKK789 pKa = 7.15QQKK792 pKa = 9.81ILTGNIADD800 pKa = 4.66LGDD803 pKa = 3.62FAGSSFGHH811 pKa = 5.51PQITIDD817 pKa = 5.04GIPLSVPATLSQNDD831 pKa = 4.03FTWSCSAVGDD841 pKa = 3.62KK842 pKa = 10.46KK843 pKa = 11.66VNIKK847 pKa = 10.15QCGLYY852 pKa = 9.02TYY854 pKa = 10.59SAIYY858 pKa = 9.9VLSPSKK864 pKa = 10.66DD865 pKa = 3.43VSHH868 pKa = 7.56LDD870 pKa = 3.45EE871 pKa = 5.03NSNKK875 pKa = 10.45LYY877 pKa = 9.75MEE879 pKa = 4.61KK880 pKa = 10.79DD881 pKa = 3.46FLVGKK886 pKa = 10.29LKK888 pKa = 10.36MVVDD892 pKa = 4.18MPKK895 pKa = 10.84EE896 pKa = 3.97MFKK899 pKa = 10.71KK900 pKa = 10.41IPTKK904 pKa = 10.41PIISEE909 pKa = 3.83AKK911 pKa = 8.95MICSGCSQCAVGIDD925 pKa = 3.78CNITYY930 pKa = 9.74TSDD933 pKa = 3.11TTFSARR939 pKa = 11.84LMMDD943 pKa = 3.59TCSFKK948 pKa = 10.77SDD950 pKa = 3.37QLGTFLGPNEE960 pKa = 4.33KK961 pKa = 10.24SIKK964 pKa = 9.84AYY966 pKa = 10.24CSEE969 pKa = 4.41EE970 pKa = 3.89ITDD973 pKa = 4.37KK974 pKa = 10.92SLKK977 pKa = 10.29LIPEE981 pKa = 4.46DD982 pKa = 4.01QEE984 pKa = 4.11EE985 pKa = 4.64LTVDD989 pKa = 3.28IQVDD993 pKa = 3.75EE994 pKa = 4.65FTQVDD999 pKa = 3.9QDD1001 pKa = 4.34TIIHH1005 pKa = 6.59FDD1007 pKa = 3.87DD1008 pKa = 5.37KK1009 pKa = 11.3SAHH1012 pKa = 6.98DD1013 pKa = 4.17EE1014 pKa = 4.3NKK1016 pKa = 9.65HH1017 pKa = 6.34HH1018 pKa = 7.3SDD1020 pKa = 3.33TSISSLWDD1028 pKa = 3.48WIKK1031 pKa = 11.43APFNWIASFFGSFFDD1046 pKa = 3.64LVRR1049 pKa = 11.84IILVIVAACVGLYY1062 pKa = 9.71IVSSIFKK1069 pKa = 10.52LSRR1072 pKa = 11.84TYY1074 pKa = 11.0YY1075 pKa = 8.94VDD1077 pKa = 3.07KK1078 pKa = 10.72RR1079 pKa = 11.84RR1080 pKa = 11.84QKK1082 pKa = 11.05LEE1084 pKa = 3.82DD1085 pKa = 4.69AIEE1088 pKa = 4.28SVEE1091 pKa = 4.65SSVLLANYY1099 pKa = 8.84TGIDD1103 pKa = 3.25QTRR1106 pKa = 11.84KK1107 pKa = 9.77RR1108 pKa = 11.84KK1109 pKa = 9.82SPPKK1113 pKa = 10.42GYY1115 pKa = 10.48DD1116 pKa = 3.1FSLDD1120 pKa = 3.12II1121 pKa = 5.22

Molecular weight:
127.34 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|Q84404|Q84404_9VIRU Non-structural protein OS=Peanut bud necrosis virus OX=40687 GN=NSm PE=4 SV=1
MM1 pKa = 7.52SNVKK5 pKa = 9.98QLTEE9 pKa = 4.08KK10 pKa = 10.51KK11 pKa = 10.05IKK13 pKa = 9.93EE14 pKa = 4.02LLAGGSADD22 pKa = 4.03VEE24 pKa = 4.45IEE26 pKa = 4.46TEE28 pKa = 4.09DD29 pKa = 3.57STPGFSFKK37 pKa = 10.96AFYY40 pKa = 8.24DD41 pKa = 3.67TNKK44 pKa = 10.16NLEE47 pKa = 4.03ITFTNCLNILKK58 pKa = 9.92CRR60 pKa = 11.84KK61 pKa = 9.63QIFAACKK68 pKa = 7.45SGKK71 pKa = 9.77YY72 pKa = 7.99VFCGKK77 pKa = 9.11TIVATNTDD85 pKa = 3.75VGPDD89 pKa = 2.87DD90 pKa = 3.44WTFKK94 pKa = 9.6RR95 pKa = 11.84TEE97 pKa = 3.6AFIRR101 pKa = 11.84TKK103 pKa = 9.04MASMVEE109 pKa = 3.99KK110 pKa = 10.61SKK112 pKa = 11.51NDD114 pKa = 3.46AAKK117 pKa = 10.37QEE119 pKa = 4.19MYY121 pKa = 10.86NKK123 pKa = 9.67IMEE126 pKa = 4.76LPLVAAYY133 pKa = 9.82GLNVPASFDD142 pKa = 3.51TCALRR147 pKa = 11.84MMLCIGGPLPLLSSMTGLAPIIFPLAYY174 pKa = 8.37YY175 pKa = 10.6QNVKK179 pKa = 10.31KK180 pKa = 10.66EE181 pKa = 3.8KK182 pKa = 10.6LGIKK186 pKa = 10.06NFSTYY191 pKa = 9.83EE192 pKa = 3.92QVCKK196 pKa = 9.96VAKK199 pKa = 10.09VLSASQIEE207 pKa = 4.69FKK209 pKa = 11.32NEE211 pKa = 3.41LEE213 pKa = 4.25EE214 pKa = 4.18MFKK217 pKa = 10.96SAVKK221 pKa = 10.36LLSEE225 pKa = 4.79SNPGTASSISLKK237 pKa = 10.73KK238 pKa = 10.13YY239 pKa = 10.29DD240 pKa = 3.9EE241 pKa = 4.12QVKK244 pKa = 10.65YY245 pKa = 9.75MDD247 pKa = 3.99KK248 pKa = 11.02AFSASLSMDD257 pKa = 3.49DD258 pKa = 4.54YY259 pKa = 11.95GEE261 pKa = 4.16HH262 pKa = 6.2SKK264 pKa = 10.97KK265 pKa = 10.23KK266 pKa = 9.73SSKK269 pKa = 10.33AGPSLEE275 pKa = 4.06LL276 pKa = 4.47

Molecular weight:
30.61 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

5020

276

2877

1004.0

114.54

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.263 ± 0.499

2.271 ± 0.484

5.876 ± 0.423

6.394 ± 0.338

4.781 ± 0.254

4.084 ± 0.321

1.813 ± 0.247

8.227 ± 0.312

9.104 ± 0.395

9.163 ± 0.378

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.307 ± 0.551

6.315 ± 0.499

2.988 ± 0.277

2.968 ± 0.071

3.127 ± 0.28

9.542 ± 0.347

5.339 ± 0.211

5.598 ± 0.545

0.797 ± 0.174

4.044 ± 0.316

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski