Acetatifactor muris

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; Acetatifactor

Average proteome isoelectric point is 6.14

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5687 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2K4ZN03|A0A2K4ZN03_9FIRM Prophage phiRv2 integrase OS=Acetatifactor muris OX=879566 GN=AMURIS_04569 PE=4 SV=1
MM1 pKa = 7.62KK2 pKa = 10.66KK3 pKa = 7.68MTALFLSLALVFGCVGCGNDD23 pKa = 4.44DD24 pKa = 3.84GQASGSSDD32 pKa = 3.09SSGTSVEE39 pKa = 4.35SSDD42 pKa = 4.88ASDD45 pKa = 3.83ASTEE49 pKa = 4.07SSTEE53 pKa = 3.89STAEE57 pKa = 3.86AQEE60 pKa = 4.4SSSEE64 pKa = 3.99ASDD67 pKa = 3.31AGEE70 pKa = 4.14DD71 pKa = 3.72VKK73 pKa = 11.44SEE75 pKa = 3.67GVMTYY80 pKa = 10.36EE81 pKa = 4.51EE82 pKa = 4.28YY83 pKa = 10.93AAAALDD89 pKa = 3.83TEE91 pKa = 4.87VVIEE95 pKa = 4.92AYY97 pKa = 10.34VQAKK101 pKa = 8.46QSWWEE106 pKa = 3.75KK107 pKa = 10.83DD108 pKa = 3.37GQGCCTVYY116 pKa = 10.94AQDD119 pKa = 3.42KK120 pKa = 10.36DD121 pKa = 3.39GGYY124 pKa = 10.46FLYY127 pKa = 10.74EE128 pKa = 4.06MACSEE133 pKa = 4.07EE134 pKa = 5.39DD135 pKa = 3.66YY136 pKa = 11.52AKK138 pKa = 10.58LVPGTKK144 pKa = 9.29IKK146 pKa = 9.86VTGFKK151 pKa = 10.81AEE153 pKa = 3.69WSGEE157 pKa = 3.88FEE159 pKa = 4.8IIDD162 pKa = 3.4AAFEE166 pKa = 4.16IGEE169 pKa = 4.13GSYY172 pKa = 10.1IAEE175 pKa = 4.53AEE177 pKa = 4.32DD178 pKa = 3.46VTALLGQDD186 pKa = 4.53DD187 pKa = 5.1LVNHH191 pKa = 5.58QNKK194 pKa = 8.7FVSFKK199 pKa = 11.12GMTVEE204 pKa = 4.33AAKK207 pKa = 10.72DD208 pKa = 3.46PEE210 pKa = 4.24GNIIKK215 pKa = 10.6DD216 pKa = 3.47EE217 pKa = 4.71AGNEE221 pKa = 3.85AAFLYY226 pKa = 10.39NWDD229 pKa = 3.9GSGQDD234 pKa = 4.14GDD236 pKa = 4.73DD237 pKa = 3.72LYY239 pKa = 11.84FNVSVNGATYY249 pKa = 8.66TFTVEE254 pKa = 5.73SYY256 pKa = 11.11LCDD259 pKa = 3.35STTDD263 pKa = 2.74VYY265 pKa = 11.6AAVKK269 pKa = 9.49GLKK272 pKa = 10.33VGDD275 pKa = 4.34KK276 pKa = 10.2IDD278 pKa = 3.83MEE280 pKa = 4.68GFLYY284 pKa = 9.77WYY286 pKa = 10.36NGVNPHH292 pKa = 5.61ITSVKK297 pKa = 9.83AANN300 pKa = 3.38

Molecular weight: 32.11 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2K4ZDR9|A0A2K4ZDR9_9FIRM 3-isopropylmalate dehydratase large subunit OS=Acetatifactor muris OX=879566 GN=dmdA_2 PE=3 SV=1
MM1 pKa = 7.68KK2 pKa = 10.19NRR4 pKa = 11.84EE5 pKa = 4.53KK6 pKa = 10.31IRR8 pKa = 11.84EE9 pKa = 4.08MTTTALLAALLAVMGMFRR27 pKa = 11.84LPSILPGCEE36 pKa = 3.88FQLSAPFAVCLAACFGFRR54 pKa = 11.84RR55 pKa = 11.84YY56 pKa = 9.88FKK58 pKa = 10.47IGVLASMMNLILGTHH73 pKa = 6.62TIVNVTIAMIFRR85 pKa = 11.84LVAGGILSLFGVNPVTIAVSGPLGTVAGRR114 pKa = 11.84VVLGAISDD122 pKa = 4.19TNPLALTAAAAPGMVFTAVGASVMYY147 pKa = 9.64PVMRR151 pKa = 11.84HH152 pKa = 4.51LVRR155 pKa = 11.84RR156 pKa = 11.84EE157 pKa = 3.6KK158 pKa = 10.97NAA160 pKa = 3.36

Molecular weight: 16.91 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5687

0

5687

1754436

29

2719

308.5

34.78

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.624 ± 0.032

1.612 ± 0.015

5.455 ± 0.028

8.128 ± 0.038

4.103 ± 0.026

7.309 ± 0.033

1.718 ± 0.015

6.606 ± 0.03

6.066 ± 0.035

9.151 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.055 ± 0.016

4.145 ± 0.025

3.377 ± 0.022

3.375 ± 0.019

5.457 ± 0.029

5.706 ± 0.038

5.143 ± 0.023

6.525 ± 0.028

1.135 ± 0.014

4.309 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski