Paenibacillus ginsengarvi
Average proteome isoelectric point is 6.45
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6998 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3B0AYY2|A0A3B0AYY2_9BACL Uncharacterized protein OS=Paenibacillus ginsengarvi OX=400777 GN=D7M11_32540 PE=4 SV=1
MM1 pKa = 7.56 SEE3 pKa = 3.79 APIYY7 pKa = 10.79 FEE9 pKa = 5.63 FSDD12 pKa = 3.65 SSAAVMALDD21 pKa = 3.72 TLSEE25 pKa = 4.3 LGYY28 pKa = 9.86 HH29 pKa = 6.16 AHH31 pKa = 6.06 ATEE34 pKa = 4.69 LDD36 pKa = 3.86 GKK38 pKa = 9.84 PGVQVYY44 pKa = 10.1 IDD46 pKa = 4.19 KK47 pKa = 11.19 NDD49 pKa = 3.42 LTSALEE55 pKa = 3.76 IAQASGGMLLSTEE68 pKa = 4.11 RR69 pKa = 11.84 AAADD73 pKa = 3.38 ADD75 pKa = 4.3 SYY77 pKa = 11.51 KK78 pKa = 10.55 LAYY81 pKa = 9.62 EE82 pKa = 4.41 LDD84 pKa = 3.93 SVPIPAHH91 pKa = 6.09 LVNEE95 pKa = 4.55 DD96 pKa = 2.7 WSEE99 pKa = 4.58 SYY101 pKa = 10.85 FSGDD105 pKa = 2.87 GAATSEE111 pKa = 4.18 AFDD114 pKa = 4.54 PSGDD118 pKa = 4.0 DD119 pKa = 3.37 YY120 pKa = 12.09 SHH122 pKa = 7.13 FSARR126 pKa = 3.65
Molecular weight: 13.46 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.747
IPC2_protein 3.935
IPC_protein 3.897
Toseland 3.694
ProMoST 4.05
Dawson 3.884
Bjellqvist 4.037
Wikipedia 3.808
Rodwell 3.732
Grimsley 3.605
Solomon 3.872
Lehninger 3.834
Nozaki 3.999
DTASelect 4.215
Thurlkill 3.745
EMBOSS 3.821
Sillero 4.024
Patrickios 0.998
IPC_peptide 3.872
IPC2_peptide 3.999
IPC2.peptide.svr19 3.895
Protein with the highest isoelectric point:
>tr|A0A3B0CMP3|A0A3B0CMP3_9BACL Carbohydrate ABC transporter permease OS=Paenibacillus ginsengarvi OX=400777 GN=D7M11_05570 PE=3 SV=1
MM1 pKa = 7.61 GPTFKK6 pKa = 10.87 PNVSKK11 pKa = 10.8 RR12 pKa = 11.84 KK13 pKa = 8.95 KK14 pKa = 8.25 VHH16 pKa = 5.49 GFRR19 pKa = 11.84 KK20 pKa = 10.01 RR21 pKa = 11.84 MSTKK25 pKa = 10.27 NGRR28 pKa = 11.84 KK29 pKa = 8.13 VLKK32 pKa = 10.34 ARR34 pKa = 11.84 RR35 pKa = 11.84 QKK37 pKa = 9.9 GRR39 pKa = 11.84 KK40 pKa = 8.85 VISAA44 pKa = 4.05
Molecular weight: 5.09 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.474
IPC2_protein 11.082
IPC_protein 12.618
Toseland 12.808
ProMoST 13.276
Dawson 12.808
Bjellqvist 12.793
Wikipedia 13.261
Rodwell 12.705
Grimsley 12.837
Solomon 13.29
Lehninger 13.188
Nozaki 12.793
DTASelect 12.793
Thurlkill 12.793
EMBOSS 13.29
Sillero 12.793
Patrickios 12.427
IPC_peptide 13.29
IPC2_peptide 12.266
IPC2.peptide.svr19 9.035
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6998
0
6998
2380545
25
3439
340.2
37.78
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.921 ± 0.035
0.84 ± 0.01
5.28 ± 0.021
6.498 ± 0.03
4.071 ± 0.019
7.875 ± 0.036
2.06 ± 0.015
5.979 ± 0.026
5.061 ± 0.031
9.801 ± 0.034
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.692 ± 0.015
3.53 ± 0.023
4.412 ± 0.018
3.571 ± 0.016
5.748 ± 0.032
6.198 ± 0.021
5.485 ± 0.025
7.066 ± 0.024
1.333 ± 0.013
3.581 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here