Sclerotinia sclerotiorum (strain ATCC 18683 / 1980 / Ss-1) (White mold) (Whetzelinia sclerotiorum)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Leotiomycetes; Helotiales; Sclerotiniaceae; Sclerotinia; Sclerotinia sclerotiorum

Average proteome isoelectric point is 6.68

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 14445 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A7EBU4|A7EBU4_SCLS1 Uncharacterized protein OS=Sclerotinia sclerotiorum (strain ATCC 18683 / 1980 / Ss-1) OX=665079 GN=SS1G_02780 PE=4 SV=1
MM1 pKa = 7.59AHH3 pKa = 7.23LEE5 pKa = 4.25PNAEE9 pKa = 3.78AQAPIRR15 pKa = 11.84RR16 pKa = 11.84RR17 pKa = 11.84MFGCEE22 pKa = 3.57FTYY25 pKa = 11.2DD26 pKa = 4.01NINHH30 pKa = 6.2FQYY33 pKa = 10.53KK34 pKa = 10.12GYY36 pKa = 10.77SFDD39 pKa = 4.02PLQEE43 pKa = 3.94PEE45 pKa = 4.32VFHH48 pKa = 6.89EE49 pKa = 4.49KK50 pKa = 11.04NNDD53 pKa = 2.7INYY56 pKa = 8.28WKK58 pKa = 10.43AQLAFRR64 pKa = 11.84GASSAGNDD72 pKa = 3.28MDD74 pKa = 4.94QIWGGDD80 pKa = 3.06EE81 pKa = 3.94WEE83 pKa = 4.39EE84 pKa = 4.49DD85 pKa = 4.18GEE87 pKa = 4.5DD88 pKa = 3.7EE89 pKa = 5.73EE90 pKa = 5.77AGEE93 pKa = 5.01WEE95 pKa = 4.56DD96 pKa = 3.51DD97 pKa = 3.38WEE99 pKa = 4.61EE100 pKa = 3.9EE101 pKa = 4.15VEE103 pKa = 4.45EE104 pKa = 4.38YY105 pKa = 10.86EE106 pKa = 5.5LYY108 pKa = 11.07DD109 pKa = 3.39EE110 pKa = 4.64VMEE113 pKa = 5.46DD114 pKa = 3.94EE115 pKa = 5.23EE116 pKa = 5.71DD117 pKa = 4.23DD118 pKa = 4.61EE119 pKa = 4.88YY120 pKa = 11.2EE121 pKa = 3.76QYY123 pKa = 11.36YY124 pKa = 10.41EE125 pKa = 4.06IGEE128 pKa = 4.24HH129 pKa = 6.64RR130 pKa = 11.84DD131 pKa = 3.16EE132 pKa = 6.84DD133 pKa = 4.15EE134 pKa = 5.46DD135 pKa = 5.21DD136 pKa = 3.9NGHH139 pKa = 7.04AGGNFEE145 pKa = 5.82GIVNLIGGGGGNLDD159 pKa = 3.18YY160 pKa = 10.58TYY162 pKa = 10.94RR163 pKa = 11.84SWRR166 pKa = 11.84LL167 pKa = 3.04

Molecular weight: 19.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A7E5V2|A7E5V2_SCLS1 Chitinase OS=Sclerotinia sclerotiorum (strain ATCC 18683 / 1980 / Ss-1) OX=665079 GN=SS1G_00677 PE=3 SV=1
MM1 pKa = 7.94PSQKK5 pKa = 10.41SFRR8 pKa = 11.84TKK10 pKa = 10.33QKK12 pKa = 10.05LARR15 pKa = 11.84AQKK18 pKa = 8.97QNRR21 pKa = 11.84PIPQWIRR28 pKa = 11.84LRR30 pKa = 11.84TGNTIRR36 pKa = 11.84YY37 pKa = 5.79NAKK40 pKa = 8.89RR41 pKa = 11.84RR42 pKa = 11.84HH43 pKa = 4.15WRR45 pKa = 11.84KK46 pKa = 7.46TRR48 pKa = 11.84IGLL51 pKa = 3.73

Molecular weight: 6.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

14445

0

14445

5243077

28

6771

363.0

40.33

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.627 ± 0.018

1.205 ± 0.009

5.524 ± 0.017

6.656 ± 0.03

3.713 ± 0.014

6.817 ± 0.023

2.268 ± 0.01

5.575 ± 0.016

5.665 ± 0.025

8.489 ± 0.023

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.285 ± 0.01

4.268 ± 0.014

5.649 ± 0.021

3.854 ± 0.019

5.752 ± 0.02

8.629 ± 0.031

6.086 ± 0.019

5.736 ± 0.017

1.381 ± 0.009

2.819 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski