Aspergillus oryzae (Yellow koji mold)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; Eurotio

Average proteome isoelectric point is 6.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 11429 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1S9DLG7|A0A1S9DLG7_ASPOZ SIT4 phosphatase-associated protein family OS=Aspergillus oryzae OX=5062 GN=OAory_01057170 PE=3 SV=1
MM1 pKa = 7.7ADD3 pKa = 3.51EE4 pKa = 5.26AGSIYY9 pKa = 10.71DD10 pKa = 3.85EE11 pKa = 4.95IEE13 pKa = 4.34IEE15 pKa = 5.79DD16 pKa = 3.79MTFDD20 pKa = 6.24PITQLYY26 pKa = 9.73HH27 pKa = 5.63YY28 pKa = 7.55PCPCGDD34 pKa = 3.67RR35 pKa = 11.84FEE37 pKa = 5.7IMIDD41 pKa = 3.49DD42 pKa = 4.19LRR44 pKa = 11.84DD45 pKa = 3.58GEE47 pKa = 4.73EE48 pKa = 3.84IAVCPSCSLRR58 pKa = 11.84IRR60 pKa = 11.84VIFDD64 pKa = 4.07LDD66 pKa = 4.0DD67 pKa = 3.49LHH69 pKa = 9.1KK70 pKa = 10.81DD71 pKa = 3.74DD72 pKa = 4.55QQQGPSAVAVQAA84 pKa = 4.09

Molecular weight: 9.49 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1S9DMJ5|A0A1S9DMJ5_ASPOZ Histone chaperone OS=Aspergillus oryzae OX=5062 GN=OAory_01060990 PE=3 SV=1
MM1 pKa = 7.14EE2 pKa = 4.95QVSQPDD8 pKa = 3.49QMNHH12 pKa = 5.02FQHH15 pKa = 6.92LNRR18 pKa = 11.84IALHH22 pKa = 5.49EE23 pKa = 4.22FVRR26 pKa = 11.84LPVSRR31 pKa = 11.84EE32 pKa = 4.03MVAHH36 pKa = 7.41LAMQASQVIRR46 pKa = 11.84CEE48 pKa = 3.84PHH50 pKa = 5.05VTTACAHH57 pKa = 5.92GQPTPPSTPPLDD69 pKa = 4.68AVDD72 pKa = 4.09SQLPPLPSVEE82 pKa = 4.15MFIASLVTRR91 pKa = 11.84SQVQVPTLMTSLVYY105 pKa = 10.42LARR108 pKa = 11.84LRR110 pKa = 11.84ARR112 pKa = 11.84LPPVAKK118 pKa = 10.25GMRR121 pKa = 11.84CTVHH125 pKa = 7.16RR126 pKa = 11.84IFLASLILAAKK137 pKa = 9.3NLNDD141 pKa = 3.64SSPKK145 pKa = 7.95NKK147 pKa = 9.25HH148 pKa = 3.66WARR151 pKa = 11.84YY152 pKa = 4.24TTVKK156 pKa = 10.64GYY158 pKa = 10.95DD159 pKa = 3.2GFAFSLPEE167 pKa = 3.85VNLMEE172 pKa = 4.37RR173 pKa = 11.84QLLFLLDD180 pKa = 3.01WDD182 pKa = 4.16TRR184 pKa = 11.84VTEE187 pKa = 4.12EE188 pKa = 5.79DD189 pKa = 4.18LLCHH193 pKa = 7.13LEE195 pKa = 4.36PFLAPIRR202 pKa = 11.84YY203 pKa = 8.88RR204 pKa = 11.84YY205 pKa = 8.93QVQEE209 pKa = 4.06RR210 pKa = 11.84EE211 pKa = 4.18AEE213 pKa = 3.97LRR215 pKa = 11.84QPRR218 pKa = 11.84EE219 pKa = 3.57WRR221 pKa = 11.84RR222 pKa = 11.84LQASAEE228 pKa = 3.98LLACRR233 pKa = 11.84LRR235 pKa = 11.84RR236 pKa = 11.84QKK238 pKa = 11.1LEE240 pKa = 3.57ARR242 pKa = 11.84LEE244 pKa = 3.93ARR246 pKa = 11.84RR247 pKa = 11.84SDD249 pKa = 3.88SIHH252 pKa = 6.1RR253 pKa = 11.84RR254 pKa = 11.84QRR256 pKa = 11.84LPASPASSVSLSSMSSMSSNASPASLADD284 pKa = 3.57TDD286 pKa = 3.38RR287 pKa = 11.84HH288 pKa = 5.3KK289 pKa = 10.59PYY291 pKa = 10.35RR292 pKa = 11.84PRR294 pKa = 11.84RR295 pKa = 11.84RR296 pKa = 11.84PSTRR300 pKa = 11.84SGASVSPPSAQDD312 pKa = 3.39VPSLTRR318 pKa = 11.84VDD320 pKa = 4.03TVPSLSSRR328 pKa = 11.84ASSIAPSSRR337 pKa = 11.84NATPASLRR345 pKa = 11.84PSSSITSMEE354 pKa = 3.45ADD356 pKa = 2.84IRR358 pKa = 11.84VVDD361 pKa = 4.64GARR364 pKa = 11.84SPSLSCGYY372 pKa = 10.53VPVSSMMAPNPKK384 pKa = 8.93MEE386 pKa = 4.42EE387 pKa = 4.1TQQPTKK393 pKa = 10.48KK394 pKa = 10.34VRR396 pKa = 11.84TNGHH400 pKa = 6.25AGHH403 pKa = 6.84TGFVARR409 pKa = 11.84FLASAAGSYY418 pKa = 9.41MGGRR422 pKa = 11.84MRR424 pKa = 11.84SHH426 pKa = 5.9VV427 pKa = 3.46

Molecular weight: 47.42 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

11429

0

11429

6065336

66

6885

530.7

58.86

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.285 ± 0.016

1.282 ± 0.008

5.635 ± 0.013

6.13 ± 0.02

3.816 ± 0.012

6.815 ± 0.022

2.432 ± 0.009

5.141 ± 0.016

4.605 ± 0.018

9.208 ± 0.019

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.146 ± 0.007

3.736 ± 0.01

5.88 ± 0.025

4.03 ± 0.015

5.949 ± 0.018

8.198 ± 0.024

5.973 ± 0.013

6.272 ± 0.017

1.521 ± 0.008

2.946 ± 0.01
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski