Amino acid dipepetide frequency for Aspergillus oryzae (Yellow koji mold)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	8.167AlaAla: 8.167 ± 0.054	1.107AlaCys: 1.107 ± 0.016	4.086AlaAsp: 4.086 ± 0.028	4.885AlaGlu: 4.885 ± 0.038	3.198AlaPhe: 3.198 ± 0.024	5.603AlaGly: 5.603 ± 0.039	1.785AlaHis: 1.785 ± 0.018	4.424AlaIle: 4.424 ± 0.03	3.742AlaLys: 3.742 ± 0.027	7.834AlaLeu: 7.834 ± 0.039	1.967AlaMet: 1.967 ± 0.02	2.896AlaAsn: 2.896 ± 0.026	4.377AlaPro: 4.377 ± 0.036	3.254AlaGln: 3.254 ± 0.027	4.664AlaArg: 4.664 ± 0.031	6.883AlaSer: 6.883 ± 0.039	5.104AlaThr: 5.104 ± 0.033	5.411AlaVal: 5.411 ± 0.031	1.183AlaTrp: 1.183 ± 0.014	2.274AlaTyr: 2.274 ± 0.02	0.0AlaXaa: 0.0 ± 0.0
Cys	1.002CysAla: 1.002 ± 0.013	0.242CysCys: 0.242 ± 0.006	0.69CysAsp: 0.69 ± 0.013	0.619CysGlu: 0.619 ± 0.01	0.567CysPhe: 0.567 ± 0.009	0.936CysGly: 0.936 ± 0.015	0.35CysHis: 0.35 ± 0.008	0.751CysIle: 0.751 ± 0.01	0.495CysLys: 0.495 ± 0.01	1.372CysLeu: 1.372 ± 0.02	0.279CysMet: 0.279 ± 0.008	0.447CysAsn: 0.447 ± 0.01	0.684CysPro: 0.684 ± 0.015	0.491CysGln: 0.491 ± 0.01	0.776CysArg: 0.776 ± 0.013	0.957CysSer: 0.957 ± 0.015	0.72CysThr: 0.72 ± 0.013	0.84CysVal: 0.84 ± 0.015	0.213CysTrp: 0.213 ± 0.006	0.392CysTyr: 0.392 ± 0.009	0.0CysXaa: 0.0 ± 0.0
Asp	4.437AspAla: 4.437 ± 0.03	0.631AspCys: 0.631 ± 0.011	3.83AspAsp: 3.83 ± 0.035	4.154AspGlu: 4.154 ± 0.037	2.17AspPhe: 2.17 ± 0.019	3.937AspGly: 3.937 ± 0.03	1.297AspHis: 1.297 ± 0.016	3.305AspIle: 3.305 ± 0.025	2.289AspLys: 2.289 ± 0.02	5.174AspLeu: 5.174 ± 0.034	1.274AspMet: 1.274 ± 0.016	1.968AspAsn: 1.968 ± 0.019	3.382AspPro: 3.382 ± 0.024	1.965AspGln: 1.965 ± 0.019	3.064AspArg: 3.064 ± 0.026	4.124AspSer: 4.124 ± 0.031	3.08AspThr: 3.08 ± 0.026	3.675AspVal: 3.675 ± 0.027	0.892AspTrp: 0.892 ± 0.015	1.707AspTyr: 1.707 ± 0.017	0.0AspXaa: 0.0 ± 0.0
Glu	5.144GluAla: 5.144 ± 0.036	0.667GluCys: 0.667 ± 0.012	4.009GluAsp: 4.009 ± 0.034	5.277GluGlu: 5.277 ± 0.055	1.965GluPhe: 1.965 ± 0.019	3.686GluGly: 3.686 ± 0.024	1.408GluHis: 1.408 ± 0.016	3.122GluIle: 3.122 ± 0.025	3.631GluLys: 3.631 ± 0.032	5.264GluLeu: 5.264 ± 0.038	1.402GluMet: 1.402 ± 0.014	2.389GluAsn: 2.389 ± 0.02	2.784GluPro: 2.784 ± 0.036	2.525GluGln: 2.525 ± 0.024	3.846GluArg: 3.846 ± 0.027	4.356GluSer: 4.356 ± 0.034	3.569GluThr: 3.569 ± 0.028	3.572GluVal: 3.572 ± 0.027	0.905GluTrp: 0.905 ± 0.013	1.78GluTyr: 1.78 ± 0.015	0.0GluXaa: 0.0 ± 0.0
Phe	3.04PheAla: 3.04 ± 0.025	0.595PheCys: 0.595 ± 0.01	2.285PheAsp: 2.285 ± 0.019	2.118PheGlu: 2.118 ± 0.019	1.71PhePhe: 1.71 ± 0.019	2.859PheGly: 2.859 ± 0.026	0.985PheHis: 0.985 ± 0.013	1.948PheIle: 1.948 ± 0.02	1.48PheLys: 1.48 ± 0.016	3.713PheLeu: 3.713 ± 0.026	0.804PheMet: 0.804 ± 0.012	1.495PheAsn: 1.495 ± 0.015	2.038PhePro: 2.038 ± 0.018	1.472PheGln: 1.472 ± 0.018	1.997PheArg: 1.997 ± 0.018	3.044PheSer: 3.044 ± 0.022	2.249PheThr: 2.249 ± 0.019	2.432PheVal: 2.432 ± 0.024	0.678PheTrp: 0.678 ± 0.011	1.214PheTyr: 1.214 ± 0.016	0.0PheXaa: 0.0 ± 0.0
Gly	5.188GlyAla: 5.188 ± 0.037	0.922GlyCys: 0.922 ± 0.015	3.606GlyAsp: 3.606 ± 0.028	3.568GlyGlu: 3.568 ± 0.026	2.895GlyPhe: 2.895 ± 0.026	5.301GlyGly: 5.301 ± 0.048	1.72GlyHis: 1.72 ± 0.019	3.737GlyIle: 3.737 ± 0.029	3.29GlyLys: 3.29 ± 0.027	6.328GlyLeu: 6.328 ± 0.033	1.543GlyMet: 1.543 ± 0.02	2.577GlyAsn: 2.577 ± 0.021	3.303GlyPro: 3.303 ± 0.027	2.611GlyGln: 2.611 ± 0.024	3.941GlyArg: 3.941 ± 0.028	5.627GlySer: 5.627 ± 0.039	3.987GlyThr: 3.987 ± 0.031	4.518GlyVal: 4.518 ± 0.03	1.222GlyTrp: 1.222 ± 0.016	2.268GlyTyr: 2.268 ± 0.02	0.0GlyXaa: 0.0 ± 0.0
His	1.833HisAla: 1.833 ± 0.018	0.358HisCys: 0.358 ± 0.008	1.345HisAsp: 1.345 ± 0.014	1.345HisGlu: 1.345 ± 0.016	0.951HisPhe: 0.951 ± 0.013	1.745HisGly: 1.745 ± 0.02	0.835HisHis: 0.835 ± 0.014	1.309HisIle: 1.309 ± 0.017	0.878HisLys: 0.878 ± 0.012	2.331HisLeu: 2.331 ± 0.021	0.509HisMet: 0.509 ± 0.011	0.88HisAsn: 0.88 ± 0.014	1.688HisPro: 1.688 ± 0.019	0.965HisGln: 0.965 ± 0.014	1.576HisArg: 1.576 ± 0.018	1.881HisSer: 1.881 ± 0.017	1.318HisThr: 1.318 ± 0.017	1.461HisVal: 1.461 ± 0.016	0.383HisTrp: 0.383 ± 0.009	0.734HisTyr: 0.734 ± 0.01	0.0HisXaa: 0.0 ± 0.0
Ile	4.351IleAla: 4.351 ± 0.029	0.811IleCys: 0.811 ± 0.012	2.915IleAsp: 2.915 ± 0.021	2.938IleGlu: 2.938 ± 0.023	2.119IlePhe: 2.119 ± 0.023	3.406IleGly: 3.406 ± 0.029	1.323IleHis: 1.323 ± 0.014	2.722IleIle: 2.722 ± 0.024	2.082IleLys: 2.082 ± 0.022	4.919IleLeu: 4.919 ± 0.03	1.069IleMet: 1.069 ± 0.013	1.876IleAsn: 1.876 ± 0.018	3.227IlePro: 3.227 ± 0.029	2.035IleGln: 2.035 ± 0.02	2.881IleArg: 2.881 ± 0.025	4.056IleSer: 4.056 ± 0.03	2.959IleThr: 2.959 ± 0.026	3.378IleVal: 3.378 ± 0.027	0.765IleTrp: 0.765 ± 0.013	1.578IleTyr: 1.578 ± 0.02	0.0IleXaa: 0.0 ± 0.0
Lys	3.962LysAla: 3.962 ± 0.03	0.517LysCys: 0.517 ± 0.01	2.756LysAsp: 2.756 ± 0.025	3.314LysGlu: 3.314 ± 0.034	1.407LysPhe: 1.407 ± 0.017	2.928LysGly: 2.928 ± 0.021	1.087LysHis: 1.087 ± 0.013	2.181LysIle: 2.181 ± 0.02	2.901LysLys: 2.901 ± 0.039	3.988LysLeu: 3.988 ± 0.032	0.9LysMet: 0.9 ± 0.013	1.727LysAsn: 1.727 ± 0.017	2.564LysPro: 2.564 ± 0.025	1.814LysGln: 1.814 ± 0.02	3.156LysArg: 3.156 ± 0.028	3.332LysSer: 3.332 ± 0.032	2.639LysThr: 2.639 ± 0.022	2.812LysVal: 2.812 ± 0.022	0.666LysTrp: 0.666 ± 0.011	1.402LysTyr: 1.402 ± 0.018	0.0LysXaa: 0.0 ± 0.0
Leu	7.826LeuAla: 7.826 ± 0.038	1.308LeuCys: 1.308 ± 0.016	5.288LeuAsp: 5.288 ± 0.03	5.638LeuGlu: 5.638 ± 0.037	3.533LeuPhe: 3.533 ± 0.032	6.203LeuGly: 6.203 ± 0.036	2.366LeuHis: 2.366 ± 0.025	4.243LeuIle: 4.243 ± 0.029	4.09LeuLys: 4.09 ± 0.031	8.867LeuLeu: 8.867 ± 0.043	1.864LeuMet: 1.864 ± 0.018	3.348LeuAsn: 3.348 ± 0.024	5.513LeuPro: 5.513 ± 0.032	3.99LeuGln: 3.99 ± 0.032	5.786LeuArg: 5.786 ± 0.037	7.67LeuSer: 7.67 ± 0.044	5.002LeuThr: 5.002 ± 0.032	5.673LeuVal: 5.673 ± 0.031	1.28LeuTrp: 1.28 ± 0.016	2.595LeuTyr: 2.595 ± 0.025	0.0LeuXaa: 0.0 ± 0.0
Met	2.096MetAla: 2.096 ± 0.018	0.269MetCys: 0.269 ± 0.008	1.229MetAsp: 1.229 ± 0.014	1.289MetGlu: 1.289 ± 0.016	0.756MetPhe: 0.756 ± 0.012	1.473MetGly: 1.473 ± 0.017	0.477MetHis: 0.477 ± 0.009	1.05MetIle: 1.05 ± 0.015	1.009MetLys: 1.009 ± 0.013	1.879MetLeu: 1.879 ± 0.022	0.536MetMet: 0.536 ± 0.01	0.825MetAsn: 0.825 ± 0.012	1.171MetPro: 1.171 ± 0.012	0.861MetGln: 0.861 ± 0.012	1.218MetArg: 1.218 ± 0.014	1.828MetSer: 1.828 ± 0.016	1.305MetThr: 1.305 ± 0.015	1.367MetVal: 1.367 ± 0.016	0.272MetTrp: 0.272 ± 0.007	0.548MetTyr: 0.548 ± 0.009	0.0MetXaa: 0.0 ± 0.0
Asn	3.141AsnAla: 3.141 ± 0.024	0.484AsnCys: 0.484 ± 0.01	2.001AsnAsp: 2.001 ± 0.018	2.087AsnGlu: 2.087 ± 0.018	1.379AsnPhe: 1.379 ± 0.016	2.97AsnGly: 2.97 ± 0.026	0.892AsnHis: 0.892 ± 0.012	2.191AsnIle: 2.191 ± 0.019	1.531AsnLys: 1.531 ± 0.017	3.351AsnLeu: 3.351 ± 0.023	0.855AsnMet: 0.855 ± 0.014	1.501AsnAsn: 1.501 ± 0.019	2.51AsnPro: 2.51 ± 0.022	1.35AsnGln: 1.35 ± 0.016	1.951AsnArg: 1.951 ± 0.018	2.74AsnSer: 2.74 ± 0.021	2.265AsnThr: 2.265 ± 0.018	2.443AsnVal: 2.443 ± 0.021	0.597AsnTrp: 0.597 ± 0.011	1.118AsnTyr: 1.118 ± 0.015	0.0AsnXaa: 0.0 ± 0.0
Pro	4.736ProAla: 4.736 ± 0.034	0.558ProCys: 0.558 ± 0.012	3.225ProAsp: 3.225 ± 0.028	3.922ProGlu: 3.922 ± 0.036	2.136ProPhe: 2.136 ± 0.019	3.856ProGly: 3.856 ± 0.034	1.289ProHis: 1.289 ± 0.015	2.59ProIle: 2.59 ± 0.021	2.502ProLys: 2.502 ± 0.023	4.788ProLeu: 4.788 ± 0.033	1.043ProMet: 1.043 ± 0.015	2.199ProAsn: 2.199 ± 0.019	4.47ProPro: 4.47 ± 0.053	2.402ProGln: 2.402 ± 0.024	3.29ProArg: 3.29 ± 0.028	5.876ProSer: 5.876 ± 0.041	3.891ProThr: 3.891 ± 0.034	3.649ProVal: 3.649 ± 0.03	0.796ProTrp: 0.796 ± 0.012	1.579ProTyr: 1.579 ± 0.018	0.0ProXaa: 0.0 ± 0.0
Gln	3.325GlnAla: 3.325 ± 0.031	0.479GlnCys: 0.479 ± 0.01	2.085GlnAsp: 2.085 ± 0.02	2.501GlnGlu: 2.501 ± 0.026	1.337GlnPhe: 1.337 ± 0.018	2.527GlnGly: 2.527 ± 0.026	1.039GlnHis: 1.039 ± 0.016	1.971GlnIle: 1.971 ± 0.02	1.985GlnLys: 1.985 ± 0.021	3.626GlnLeu: 3.626 ± 0.026	0.873GlnMet: 0.873 ± 0.012	1.602GlnAsn: 1.602 ± 0.018	2.472GlnPro: 2.472 ± 0.028	2.238GlnGln: 2.238 ± 0.034	2.588GlnArg: 2.588 ± 0.022	3.189GlnSer: 3.189 ± 0.028	2.388GlnThr: 2.388 ± 0.02	2.24GlnVal: 2.24 ± 0.02	0.615GlnTrp: 0.615 ± 0.01	1.223GlnTyr: 1.223 ± 0.016	0.0GlnXaa: 0.0 ± 0.0
Arg	4.467ArgAla: 4.467 ± 0.033	0.704ArgCys: 0.704 ± 0.013	3.279ArgAsp: 3.279 ± 0.026	3.739ArgGlu: 3.739 ± 0.031	2.222ArgPhe: 2.222 ± 0.018	3.553ArgGly: 3.553 ± 0.033	1.532ArgHis: 1.532 ± 0.019	2.923ArgIle: 2.923 ± 0.022	3.254ArgLys: 3.254 ± 0.028	5.62ArgLeu: 5.62 ± 0.037	1.262ArgMet: 1.262 ± 0.013	2.238ArgAsn: 2.238 ± 0.019	3.308ArgPro: 3.308 ± 0.029	2.575ArgGln: 2.575 ± 0.024	4.801ArgArg: 4.801 ± 0.042	4.632ArgSer: 4.632 ± 0.035	3.215ArgThr: 3.215 ± 0.025	3.435ArgVal: 3.435 ± 0.023	0.979ArgTrp: 0.979 ± 0.012	1.758ArgTyr: 1.758 ± 0.019	0.0ArgXaa: 0.0 ± 0.0
Ser	6.414SerAla: 6.414 ± 0.036	0.92SerCys: 0.92 ± 0.013	4.287SerAsp: 4.287 ± 0.031	4.299SerGlu: 4.299 ± 0.031	3.1SerPhe: 3.1 ± 0.02	5.506SerGly: 5.506 ± 0.035	1.98SerHis: 1.98 ± 0.02	4.115SerIle: 4.115 ± 0.027	3.579SerLys: 3.579 ± 0.026	7.518SerLeu: 7.518 ± 0.035	1.703SerMet: 1.703 ± 0.019	3.043SerAsn: 3.043 ± 0.026	5.245SerPro: 5.245 ± 0.043	3.367SerGln: 3.367 ± 0.027	4.825SerArg: 4.825 ± 0.038	8.45SerSer: 8.45 ± 0.067	5.48SerThr: 5.48 ± 0.039	4.778SerVal: 4.778 ± 0.032	1.186SerTrp: 1.186 ± 0.016	2.186SerTyr: 2.186 ± 0.021	0.0SerXaa: 0.0 ± 0.0
Thr	5.094ThrAla: 5.094 ± 0.028	0.756ThrCys: 0.756 ± 0.011	2.988ThrAsp: 2.988 ± 0.024	3.272ThrGlu: 3.272 ± 0.026	2.307ThrPhe: 2.307 ± 0.02	4.303ThrGly: 4.303 ± 0.033	1.314ThrHis: 1.314 ± 0.017	3.236ThrIle: 3.236 ± 0.023	2.543ThrLys: 2.543 ± 0.021	5.404ThrLeu: 5.404 ± 0.033	1.207ThrMet: 1.207 ± 0.013	2.128ThrAsn: 2.128 ± 0.02	4.15ThrPro: 4.15 ± 0.034	2.115ThrGln: 2.115 ± 0.019	2.979ThrArg: 2.979 ± 0.021	5.204ThrSer: 5.204 ± 0.035	4.137ThrThr: 4.137 ± 0.037	3.988ThrVal: 3.988 ± 0.028	0.903ThrTrp: 0.903 ± 0.012	1.701ThrTyr: 1.701 ± 0.018	0.0ThrXaa: 0.0 ± 0.0
Val	5.217ValAla: 5.217 ± 0.033	0.863ValCys: 0.863 ± 0.013	3.804ValAsp: 3.804 ± 0.023	3.845ValGlu: 3.845 ± 0.026	2.573ValPhe: 2.573 ± 0.022	4.159ValGly: 4.159 ± 0.03	1.467ValHis: 1.467 ± 0.016	3.184ValIle: 3.184 ± 0.026	2.796ValLys: 2.796 ± 0.024	5.797ValLeu: 5.797 ± 0.034	1.335ValMet: 1.335 ± 0.015	2.347ValAsn: 2.347 ± 0.021	3.665ValPro: 3.665 ± 0.029	2.48ValGln: 2.48 ± 0.021	3.442ValArg: 3.442 ± 0.028	4.895ValSer: 4.895 ± 0.033	3.704ValThr: 3.704 ± 0.025	4.359ValVal: 4.359 ± 0.034	0.895ValTrp: 0.895 ± 0.012	1.889ValTyr: 1.889 ± 0.019	0.0ValXaa: 0.0 ± 0.0
Trp	1.156TrpAla: 1.156 ± 0.014	0.201TrpCys: 0.201 ± 0.006	0.943TrpAsp: 0.943 ± 0.014	0.88TrpGlu: 0.88 ± 0.012	0.572TrpPhe: 0.572 ± 0.009	0.976TrpGly: 0.976 ± 0.014	0.371TrpHis: 0.371 ± 0.008	0.826TrpIle: 0.826 ± 0.013	0.848TrpLys: 0.848 ± 0.012	1.444TrpLeu: 1.444 ± 0.017	0.379TrpMet: 0.379 ± 0.008	0.674TrpAsn: 0.674 ± 0.012	0.634TrpPro: 0.634 ± 0.011	0.586TrpGln: 0.586 ± 0.01	0.973TrpArg: 0.973 ± 0.015	1.09TrpSer: 1.09 ± 0.014	0.958TrpThr: 0.958 ± 0.012	0.936TrpVal: 0.936 ± 0.012	0.297TrpTrp: 0.297 ± 0.008	0.469TrpTyr: 0.469 ± 0.009	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.249TyrAla: 2.249 ± 0.021	0.431TyrCys: 0.431 ± 0.009	1.702TyrAsp: 1.702 ± 0.017	1.614TyrGlu: 1.614 ± 0.017	1.259TyrPhe: 1.259 ± 0.014	2.226TyrGly: 2.226 ± 0.024	0.808TyrHis: 0.808 ± 0.011	1.58TyrIle: 1.58 ± 0.017	1.126TyrLys: 1.126 ± 0.014	2.866TyrLeu: 2.866 ± 0.024	0.653TyrMet: 0.653 ± 0.01	1.199TyrAsn: 1.199 ± 0.015	1.612TyrPro: 1.612 ± 0.02	1.205TyrGln: 1.205 ± 0.016	1.741TyrArg: 1.741 ± 0.016	2.149TyrSer: 2.149 ± 0.021	1.759TyrThr: 1.759 ± 0.018	1.749TyrVal: 1.749 ± 0.018	0.485TyrTrp: 0.485 ± 0.009	1.042TyrTyr: 1.042 ± 0.03	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 11429 proteins (6065336 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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