Staphylococcus phage VB-SauS-SA2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Sextaecvirus; unclassified Sextaecvirus

Average proteome isoelectric point is 5.76

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 130 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2Z4Q0L2|A0A2Z4Q0L2_9CAUD Uncharacterized protein OS=Staphylococcus phage VB-SauS-SA2 OX=2234082 PE=4 SV=1
MM1 pKa = 7.42NEE3 pKa = 3.74QGITLEE9 pKa = 3.92NLDD12 pKa = 3.85YY13 pKa = 11.34YY14 pKa = 10.76RR15 pKa = 11.84LYY17 pKa = 10.08WLSDD21 pKa = 3.56FNGEE25 pKa = 4.07EE26 pKa = 4.06VGFPDD31 pKa = 4.11SNVVGHH37 pKa = 5.75YY38 pKa = 7.61MHH40 pKa = 7.37GEE42 pKa = 3.74LNLYY46 pKa = 9.87IDD48 pKa = 4.02TEE50 pKa = 4.63TNSVLEE56 pKa = 3.95LWFNEE61 pKa = 3.81IEE63 pKa = 4.17EE64 pKa = 4.62EE65 pKa = 4.09II66 pKa = 4.37

Molecular weight: 7.85 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2Z4Q0L4|A0A2Z4Q0L4_9CAUD Putative D(1) dopamine receptor-like protein OS=Staphylococcus phage VB-SauS-SA2 OX=2234082 PE=4 SV=1
MM1 pKa = 7.61KK2 pKa = 10.2FRR4 pKa = 11.84YY5 pKa = 9.25KK6 pKa = 10.26GKK8 pKa = 10.43KK9 pKa = 8.13YY10 pKa = 9.15RR11 pKa = 11.84TRR13 pKa = 11.84KK14 pKa = 8.47WIDD17 pKa = 3.0YY18 pKa = 7.67TAISTVIGVVFWSYY32 pKa = 10.03VIWYY36 pKa = 8.39FPLL39 pKa = 5.38

Molecular weight: 4.91 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

130

0

130

26087

39

2332

200.7

23.0

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.845 ± 0.35

0.59 ± 0.084

6.789 ± 0.33

8.613 ± 0.451

3.99 ± 0.16

6.202 ± 0.419

1.667 ± 0.116

7.739 ± 0.229

8.809 ± 0.334

7.897 ± 0.254

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.936 ± 0.142

6.639 ± 0.168

2.277 ± 0.136

3.335 ± 0.193

3.619 ± 0.147

6.195 ± 0.245

5.673 ± 0.243

6.218 ± 0.185

1.211 ± 0.185

4.753 ± 0.311
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski