Amino acid dipepetide frequency for Staphylococcus phage VB-SauS-SA2

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
1.802AlaAla: 1.802 ± 0.467
0.153AlaCys: 0.153 ± 0.067
3.105AlaAsp: 3.105 ± 0.33
3.488AlaGlu: 3.488 ± 0.424
1.763AlaPhe: 1.763 ± 0.274
3.143AlaGly: 3.143 ± 0.453
0.69AlaHis: 0.69 ± 0.139
3.91AlaIle: 3.91 ± 0.413
3.028AlaLys: 3.028 ± 0.462
4.485AlaLeu: 4.485 ± 0.515
2.453AlaMet: 2.453 ± 0.371
2.837AlaAsn: 2.837 ± 0.305
1.035AlaPro: 1.035 ± 0.188
1.687AlaGln: 1.687 ± 0.27
2.032AlaArg: 2.032 ± 0.283
3.68AlaSer: 3.68 ± 0.607
3.028AlaThr: 3.028 ± 0.361
3.143AlaVal: 3.143 ± 0.404
0.613AlaTrp: 0.613 ± 0.168
2.377AlaTyr: 2.377 ± 0.368
0.0AlaXaa: 0.0 ± 0.0
Cys
0.307CysAla: 0.307 ± 0.12
0.038CysCys: 0.038 ± 0.034
0.268CysAsp: 0.268 ± 0.093
0.422CysGlu: 0.422 ± 0.14
0.268CysPhe: 0.268 ± 0.099
0.345CysGly: 0.345 ± 0.125
0.077CysHis: 0.077 ± 0.054
0.575CysIle: 0.575 ± 0.149
0.23CysLys: 0.23 ± 0.114
0.575CysLeu: 0.575 ± 0.15
0.115CysMet: 0.115 ± 0.064
0.498CysAsn: 0.498 ± 0.163
0.153CysPro: 0.153 ± 0.095
0.268CysGln: 0.268 ± 0.09
0.153CysArg: 0.153 ± 0.079
0.345CysSer: 0.345 ± 0.103
0.345CysThr: 0.345 ± 0.128
0.268CysVal: 0.268 ± 0.113
0.115CysTrp: 0.115 ± 0.064
0.537CysTyr: 0.537 ± 0.162
0.0CysXaa: 0.0 ± 0.0
Asp
2.99AspAla: 2.99 ± 0.32
0.345AspCys: 0.345 ± 0.13
4.102AspAsp: 4.102 ± 0.523
5.444AspGlu: 5.444 ± 0.512
3.373AspPhe: 3.373 ± 0.478
5.022AspGly: 5.022 ± 0.493
0.46AspHis: 0.46 ± 0.145
6.632AspIle: 6.632 ± 0.578
6.67AspLys: 6.67 ± 0.583
5.367AspLeu: 5.367 ± 0.447
2.683AspMet: 2.683 ± 0.321
3.795AspAsn: 3.795 ± 0.481
1.418AspPro: 1.418 ± 0.265
1.15AspGln: 1.15 ± 0.196
2.032AspArg: 2.032 ± 0.325
3.948AspSer: 3.948 ± 0.438
3.412AspThr: 3.412 ± 0.461
4.102AspVal: 4.102 ± 0.422
1.112AspTrp: 1.112 ± 0.217
3.833AspTyr: 3.833 ± 0.551
0.0AspXaa: 0.0 ± 0.0
Glu
3.335GluAla: 3.335 ± 0.464
0.843GluCys: 0.843 ± 0.183
6.172GluAsp: 6.172 ± 0.657
8.472GluGlu: 8.472 ± 0.984
3.603GluPhe: 3.603 ± 0.444
4.6GluGly: 4.6 ± 0.579
0.92GluHis: 0.92 ± 0.206
6.249GluIle: 6.249 ± 0.745
7.284GluLys: 7.284 ± 0.604
7.475GluLeu: 7.475 ± 0.626
3.182GluMet: 3.182 ± 0.401
5.904GluAsn: 5.904 ± 0.611
1.725GluPro: 1.725 ± 0.324
3.488GluGln: 3.488 ± 0.386
3.335GluArg: 3.335 ± 0.37
4.562GluSer: 4.562 ± 0.483
3.642GluThr: 3.642 ± 0.38
5.405GluVal: 5.405 ± 0.591
1.38GluTrp: 1.38 ± 0.222
4.562GluTyr: 4.562 ± 0.487
0.0GluXaa: 0.0 ± 0.0
Phe
1.802PheAla: 1.802 ± 0.27
0.345PheCys: 0.345 ± 0.125
2.913PheAsp: 2.913 ± 0.385
3.028PheGlu: 3.028 ± 0.405
1.495PhePhe: 1.495 ± 0.273
2.377PheGly: 2.377 ± 0.347
0.882PheHis: 0.882 ± 0.194
3.297PheIle: 3.297 ± 0.567
4.025PheLys: 4.025 ± 0.376
2.875PheLeu: 2.875 ± 0.388
0.882PheMet: 0.882 ± 0.19
3.028PheAsn: 3.028 ± 0.359
0.92PhePro: 0.92 ± 0.179
0.767PheGln: 0.767 ± 0.195
1.763PheArg: 1.763 ± 0.238
2.108PheSer: 2.108 ± 0.266
1.955PheThr: 1.955 ± 0.29
2.492PheVal: 2.492 ± 0.327
0.422PheTrp: 0.422 ± 0.132
2.53PheTyr: 2.53 ± 0.318
0.0PheXaa: 0.0 ± 0.0
Gly
3.105GlyAla: 3.105 ± 0.472
0.307GlyCys: 0.307 ± 0.119
3.488GlyAsp: 3.488 ± 0.322
3.987GlyGlu: 3.987 ± 0.404
3.143GlyPhe: 3.143 ± 0.34
3.067GlyGly: 3.067 ± 0.567
1.265GlyHis: 1.265 ± 0.209
5.329GlyIle: 5.329 ± 0.477
5.252GlyLys: 5.252 ± 0.523
5.137GlyLeu: 5.137 ± 0.608
1.725GlyMet: 1.725 ± 0.292
4.255GlyAsn: 4.255 ± 0.584
1.112GlyPro: 1.112 ± 0.348
2.147GlyGln: 2.147 ± 0.31
2.07GlyArg: 2.07 ± 0.264
4.063GlySer: 4.063 ± 0.542
3.565GlyThr: 3.565 ± 0.593
4.6GlyVal: 4.6 ± 0.459
1.035GlyTrp: 1.035 ± 0.295
3.373GlyTyr: 3.373 ± 0.433
0.0GlyXaa: 0.0 ± 0.0
His
0.805HisAla: 0.805 ± 0.202
0.115HisCys: 0.115 ± 0.066
1.342HisAsp: 1.342 ± 0.277
1.035HisGlu: 1.035 ± 0.248
0.652HisPhe: 0.652 ± 0.176
1.265HisGly: 1.265 ± 0.176
0.652HisHis: 0.652 ± 0.146
1.572HisIle: 1.572 ± 0.31
1.112HisLys: 1.112 ± 0.206
1.265HisLeu: 1.265 ± 0.208
0.46HisMet: 0.46 ± 0.112
0.997HisAsn: 0.997 ± 0.177
0.46HisPro: 0.46 ± 0.113
0.498HisGln: 0.498 ± 0.166
0.652HisArg: 0.652 ± 0.155
0.728HisSer: 0.728 ± 0.177
0.92HisThr: 0.92 ± 0.194
1.265HisVal: 1.265 ± 0.179
0.077HisTrp: 0.077 ± 0.056
0.805HisTyr: 0.805 ± 0.202
0.0HisXaa: 0.0 ± 0.0
Ile
3.603IleAla: 3.603 ± 0.379
0.345IleCys: 0.345 ± 0.106
5.635IleAsp: 5.635 ± 0.41
7.245IleGlu: 7.245 ± 0.717
2.607IlePhe: 2.607 ± 0.287
4.447IleGly: 4.447 ± 0.423
1.035IleHis: 1.035 ± 0.175
5.712IleIle: 5.712 ± 0.636
8.28IleLys: 8.28 ± 0.721
5.367IleLeu: 5.367 ± 0.477
2.683IleMet: 2.683 ± 0.285
4.907IleAsn: 4.907 ± 0.51
2.645IlePro: 2.645 ± 0.327
2.415IleGln: 2.415 ± 0.352
2.683IleArg: 2.683 ± 0.304
4.869IleSer: 4.869 ± 0.378
4.6IleThr: 4.6 ± 0.437
4.447IleVal: 4.447 ± 0.575
1.112IleTrp: 1.112 ± 0.541
2.76IleTyr: 2.76 ± 0.378
0.0IleXaa: 0.0 ± 0.0
Lys
4.6LysAla: 4.6 ± 0.935
0.383LysCys: 0.383 ± 0.121
6.824LysAsp: 6.824 ± 0.489
9.315LysGlu: 9.315 ± 0.749
2.722LysPhe: 2.722 ± 0.343
6.287LysGly: 6.287 ± 0.85
1.878LysHis: 1.878 ± 0.255
5.75LysIle: 5.75 ± 0.455
7.322LysLys: 7.322 ± 0.537
6.019LysLeu: 6.019 ± 0.521
2.492LysMet: 2.492 ± 0.318
5.98LysAsn: 5.98 ± 0.481
1.763LysPro: 1.763 ± 0.35
3.565LysGln: 3.565 ± 0.414
3.68LysArg: 3.68 ± 0.411
4.408LysSer: 4.408 ± 0.548
4.792LysThr: 4.792 ± 0.469
5.444LysVal: 5.444 ± 0.535
0.652LysTrp: 0.652 ± 0.161
4.178LysTyr: 4.178 ± 0.367
0.0LysXaa: 0.0 ± 0.0
Leu
3.948LeuAla: 3.948 ± 0.427
0.345LeuCys: 0.345 ± 0.136
6.325LeuAsp: 6.325 ± 0.667
8.204LeuGlu: 8.204 ± 0.859
3.028LeuPhe: 3.028 ± 0.417
3.987LeuGly: 3.987 ± 0.451
1.572LeuHis: 1.572 ± 0.25
5.29LeuIle: 5.29 ± 0.507
7.322LeuLys: 7.322 ± 0.583
6.249LeuLeu: 6.249 ± 0.482
2.223LeuMet: 2.223 ± 0.291
4.562LeuAsn: 4.562 ± 0.543
1.457LeuPro: 1.457 ± 0.26
3.105LeuGln: 3.105 ± 0.312
2.722LeuArg: 2.722 ± 0.418
5.98LeuSer: 5.98 ± 0.527
4.485LeuThr: 4.485 ± 0.425
4.025LeuVal: 4.025 ± 0.435
0.958LeuTrp: 0.958 ± 0.213
3.182LeuTyr: 3.182 ± 0.434
0.0LeuXaa: 0.0 ± 0.0
Met
1.917MetAla: 1.917 ± 0.511
0.077MetCys: 0.077 ± 0.053
1.955MetAsp: 1.955 ± 0.286
3.067MetGlu: 3.067 ± 0.436
0.997MetPhe: 0.997 ± 0.182
1.648MetGly: 1.648 ± 0.395
0.345MetHis: 0.345 ± 0.104
2.262MetIle: 2.262 ± 0.287
2.913MetLys: 2.913 ± 0.369
1.878MetLeu: 1.878 ± 0.246
0.805MetMet: 0.805 ± 0.18
2.53MetAsn: 2.53 ± 0.259
0.652MetPro: 0.652 ± 0.166
0.92MetGln: 0.92 ± 0.202
0.537MetArg: 0.537 ± 0.143
1.84MetSer: 1.84 ± 0.271
1.917MetThr: 1.917 ± 0.248
1.878MetVal: 1.878 ± 0.261
0.307MetTrp: 0.307 ± 0.106
0.92MetTyr: 0.92 ± 0.186
0.0MetXaa: 0.0 ± 0.0
Asn
3.488AsnAla: 3.488 ± 0.423
0.383AsnCys: 0.383 ± 0.116
4.255AsnAsp: 4.255 ± 0.401
5.405AsnGlu: 5.405 ± 0.591
2.377AsnPhe: 2.377 ± 0.341
4.869AsnGly: 4.869 ± 0.466
1.15AsnHis: 1.15 ± 0.232
4.945AsnIle: 4.945 ± 0.359
6.364AsnLys: 6.364 ± 0.527
5.52AsnLeu: 5.52 ± 0.482
2.147AsnMet: 2.147 ± 0.287
4.523AsnAsn: 4.523 ± 0.49
1.533AsnPro: 1.533 ± 0.215
2.3AsnGln: 2.3 ± 0.266
2.645AsnArg: 2.645 ± 0.336
3.948AsnSer: 3.948 ± 0.417
4.063AsnThr: 4.063 ± 0.426
3.335AsnVal: 3.335 ± 0.352
0.69AsnTrp: 0.69 ± 0.19
2.453AsnTyr: 2.453 ± 0.332
0.0AsnXaa: 0.0 ± 0.0
Pro
0.997ProAla: 0.997 ± 0.24
0.077ProCys: 0.077 ± 0.049
1.572ProAsp: 1.572 ± 0.218
2.185ProGlu: 2.185 ± 0.334
1.073ProPhe: 1.073 ± 0.167
0.958ProGly: 0.958 ± 0.31
0.46ProHis: 0.46 ± 0.115
1.763ProIle: 1.763 ± 0.247
1.572ProLys: 1.572 ± 0.255
1.38ProLeu: 1.38 ± 0.212
0.537ProMet: 0.537 ± 0.144
1.342ProAsn: 1.342 ± 0.275
0.383ProPro: 0.383 ± 0.092
0.997ProGln: 0.997 ± 0.242
0.882ProArg: 0.882 ± 0.203
1.687ProSer: 1.687 ± 0.195
1.725ProThr: 1.725 ± 0.265
1.61ProVal: 1.61 ± 0.262
0.077ProTrp: 0.077 ± 0.059
1.495ProTyr: 1.495 ± 0.218
0.0ProXaa: 0.0 ± 0.0
Gln
1.687GlnAla: 1.687 ± 0.248
0.268GlnCys: 0.268 ± 0.111
2.185GlnAsp: 2.185 ± 0.267
3.488GlnGlu: 3.488 ± 0.413
0.882GlnPhe: 0.882 ± 0.201
2.338GlnGly: 2.338 ± 0.627
0.805GlnHis: 0.805 ± 0.199
2.3GlnIle: 2.3 ± 0.252
2.568GlnLys: 2.568 ± 0.336
3.182GlnLeu: 3.182 ± 0.413
0.843GlnMet: 0.843 ± 0.275
1.533GlnAsn: 1.533 ± 0.244
0.575GlnPro: 0.575 ± 0.205
1.648GlnGln: 1.648 ± 0.293
1.533GlnArg: 1.533 ± 0.256
1.725GlnSer: 1.725 ± 0.274
1.725GlnThr: 1.725 ± 0.29
1.917GlnVal: 1.917 ± 0.278
0.383GlnTrp: 0.383 ± 0.108
1.763GlnTyr: 1.763 ± 0.247
0.0GlnXaa: 0.0 ± 0.0
Arg
2.108ArgAla: 2.108 ± 0.247
0.115ArgCys: 0.115 ± 0.072
2.53ArgAsp: 2.53 ± 0.342
3.105ArgGlu: 3.105 ± 0.464
1.61ArgPhe: 1.61 ± 0.27
2.683ArgGly: 2.683 ± 0.377
0.843ArgHis: 0.843 ± 0.141
2.722ArgIle: 2.722 ± 0.308
2.952ArgLys: 2.952 ± 0.393
2.722ArgLeu: 2.722 ± 0.334
1.035ArgMet: 1.035 ± 0.21
2.338ArgAsn: 2.338 ± 0.385
0.997ArgPro: 0.997 ± 0.248
1.188ArgGln: 1.188 ± 0.218
1.61ArgArg: 1.61 ± 0.242
1.725ArgSer: 1.725 ± 0.296
1.725ArgThr: 1.725 ± 0.217
2.032ArgVal: 2.032 ± 0.273
0.268ArgTrp: 0.268 ± 0.11
1.878ArgTyr: 1.878 ± 0.317
0.0ArgXaa: 0.0 ± 0.0
Ser
3.603SerAla: 3.603 ± 0.469
0.537SerCys: 0.537 ± 0.218
4.102SerAsp: 4.102 ± 0.423
4.945SerGlu: 4.945 ± 0.551
2.798SerPhe: 2.798 ± 0.264
3.718SerGly: 3.718 ± 0.55
0.728SerHis: 0.728 ± 0.155
5.214SerIle: 5.214 ± 0.423
5.214SerLys: 5.214 ± 0.521
5.29SerLeu: 5.29 ± 0.44
1.265SerMet: 1.265 ± 0.24
3.987SerAsn: 3.987 ± 0.462
1.418SerPro: 1.418 ± 0.229
1.84SerGln: 1.84 ± 0.255
2.147SerArg: 2.147 ± 0.285
3.987SerSer: 3.987 ± 0.503
3.603SerThr: 3.603 ± 0.423
3.91SerVal: 3.91 ± 0.501
0.728SerTrp: 0.728 ± 0.178
2.913SerTyr: 2.913 ± 0.395
0.0SerXaa: 0.0 ± 0.0
Thr
3.028ThrAla: 3.028 ± 0.449
0.23ThrCys: 0.23 ± 0.095
2.875ThrAsp: 2.875 ± 0.323
4.37ThrGlu: 4.37 ± 0.424
2.415ThrPhe: 2.415 ± 0.33
4.523ThrGly: 4.523 ± 0.718
0.958ThrHis: 0.958 ± 0.187
4.063ThrIle: 4.063 ± 0.418
4.792ThrLys: 4.792 ± 0.504
4.178ThrLeu: 4.178 ± 0.527
0.92ThrMet: 0.92 ± 0.201
3.872ThrAsn: 3.872 ± 0.488
1.763ThrPro: 1.763 ± 0.248
2.032ThrGln: 2.032 ± 0.349
1.84ThrArg: 1.84 ± 0.322
3.527ThrSer: 3.527 ± 0.407
4.562ThrThr: 4.562 ± 1.222
4.255ThrVal: 4.255 ± 0.407
0.537ThrTrp: 0.537 ± 0.217
1.993ThrTyr: 1.993 ± 0.297
0.0ThrXaa: 0.0 ± 0.0
Val
2.798ValAla: 2.798 ± 0.382
0.383ValCys: 0.383 ± 0.139
3.948ValAsp: 3.948 ± 0.471
4.37ValGlu: 4.37 ± 0.463
2.492ValPhe: 2.492 ± 0.359
3.565ValGly: 3.565 ± 0.394
0.958ValHis: 0.958 ± 0.181
4.907ValIle: 4.907 ± 0.431
6.632ValLys: 6.632 ± 0.62
4.945ValLeu: 4.945 ± 0.384
1.342ValMet: 1.342 ± 0.233
4.677ValAsn: 4.677 ± 0.431
1.61ValPro: 1.61 ± 0.213
1.917ValGln: 1.917 ± 0.304
1.763ValArg: 1.763 ± 0.241
4.792ValSer: 4.792 ± 0.442
3.105ValThr: 3.105 ± 0.482
4.293ValVal: 4.293 ± 0.376
0.69ValTrp: 0.69 ± 0.311
2.99ValTyr: 2.99 ± 0.344
0.0ValXaa: 0.0 ± 0.0
Trp
0.498TrpAla: 0.498 ± 0.154
0.115TrpCys: 0.115 ± 0.067
0.498TrpAsp: 0.498 ± 0.138
1.112TrpGlu: 1.112 ± 0.233
0.46TrpPhe: 0.46 ± 0.15
0.92TrpGly: 0.92 ± 0.335
0.268TrpHis: 0.268 ± 0.099
1.112TrpIle: 1.112 ± 0.323
0.805TrpLys: 0.805 ± 0.301
0.652TrpLeu: 0.652 ± 0.155
0.077TrpMet: 0.077 ± 0.053
0.958TrpAsn: 0.958 ± 0.419
0.0TrpPro: 0.0 ± 0.0
0.46TrpGln: 0.46 ± 0.105
0.46TrpArg: 0.46 ± 0.102
1.035TrpSer: 1.035 ± 0.228
0.92TrpThr: 0.92 ± 0.265
0.92TrpVal: 0.92 ± 0.188
0.192TrpTrp: 0.192 ± 0.087
0.652TrpTyr: 0.652 ± 0.155
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.032TyrAla: 2.032 ± 0.315
0.498TyrCys: 0.498 ± 0.151
3.795TyrAsp: 3.795 ± 0.462
3.45TyrGlu: 3.45 ± 0.48
2.147TyrPhe: 2.147 ± 0.374
2.262TyrGly: 2.262 ± 0.393
0.728TyrHis: 0.728 ± 0.198
3.795TyrIle: 3.795 ± 0.505
3.757TyrLys: 3.757 ± 0.371
4.408TyrLeu: 4.408 ± 0.545
1.457TyrMet: 1.457 ± 0.206
3.872TyrAsn: 3.872 ± 0.549
1.15TyrPro: 1.15 ± 0.234
0.958TyrGln: 0.958 ± 0.169
1.648TyrArg: 1.648 ± 0.32
2.99TyrSer: 2.99 ± 0.38
2.645TyrThr: 2.645 ± 0.294
2.837TyrVal: 2.837 ± 0.369
0.767TyrTrp: 0.767 ± 0.188
2.338TyrTyr: 2.338 ± 0.393
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 130 proteins (26087 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski