Cricetid gammaherpesvirus 2
Average proteome isoelectric point is 6.71
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 82 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|E9M5L4|E9M5L4_9GAMA Uncharacterized protein OS=Cricetid gammaherpesvirus 2 OX=1605972 GN=RHVP-L.31 PE=3 SV=1
MM1 pKa = 7.59 SGPEE5 pKa = 3.72 CSEE8 pKa = 4.05 KK9 pKa = 10.64 KK10 pKa = 10.33 KK11 pKa = 9.6 EE12 pKa = 4.26 TISDD16 pKa = 3.58 NDD18 pKa = 3.36 MEE20 pKa = 4.4 EE21 pKa = 4.19 CMKK24 pKa = 10.43 FFTMPLPQMLHH35 pKa = 7.33 AINQSIADD43 pKa = 3.76 FEE45 pKa = 4.84 IVCSASQQLEE55 pKa = 4.29 RR56 pKa = 11.84 ACLLFDD62 pKa = 4.14 FVGAEE67 pKa = 4.22 CVKK70 pKa = 10.57 DD71 pKa = 3.6 VGMEE75 pKa = 3.84 THH77 pKa = 7.17 AEE79 pKa = 3.86
Molecular weight: 8.87 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.351
IPC2_protein 4.431
IPC_protein 4.291
Toseland 4.151
ProMoST 4.329
Dawson 4.24
Bjellqvist 4.457
Wikipedia 4.101
Rodwell 4.139
Grimsley 4.062
Solomon 4.228
Lehninger 4.177
Nozaki 4.355
DTASelect 4.457
Thurlkill 4.151
EMBOSS 4.113
Sillero 4.406
Patrickios 3.35
IPC_peptide 4.24
IPC2_peptide 4.393
IPC2.peptide.svr19 4.338
Protein with the highest isoelectric point:
>tr|E9M5K4|E9M5K4_9GAMA Thymidine kinase OS=Cricetid gammaherpesvirus 2 OX=1605972 GN=RHVP-L.21 PE=3 SV=1
MM1 pKa = 7.77 AALHH5 pKa = 6.38 KK6 pKa = 10.48 NWAKK10 pKa = 8.73 QTLAQLPPKK19 pKa = 9.88 RR20 pKa = 11.84 KK21 pKa = 9.28 AVGKK25 pKa = 9.56 RR26 pKa = 11.84 AHH28 pKa = 6.22 LRR30 pKa = 11.84 CYY32 pKa = 9.84 IQLLRR37 pKa = 11.84 EE38 pKa = 4.28 PTLHH42 pKa = 7.46 ALLDD46 pKa = 3.7 QLKK49 pKa = 10.0 ISHH52 pKa = 7.1 TDD54 pKa = 2.88 GCHH57 pKa = 5.9 QLFKK61 pKa = 11.0 LYY63 pKa = 10.71 FEE65 pKa = 4.53 VRR67 pKa = 11.84 LGHH70 pKa = 6.31 RR71 pKa = 11.84 VADD74 pKa = 4.76 AIILTGNDD82 pKa = 4.22 SLCHH86 pKa = 6.12 CYY88 pKa = 10.0 ILEE91 pKa = 4.49 FKK93 pKa = 9.79 TCASPKK99 pKa = 9.98 FDD101 pKa = 3.75 INCQIRR107 pKa = 11.84 AAQHH111 pKa = 6.88 AEE113 pKa = 4.0 GCSQLRR119 pKa = 11.84 DD120 pKa = 3.86 MLSHH124 pKa = 6.76 LRR126 pKa = 11.84 WHH128 pKa = 6.33 VPAGPEE134 pKa = 3.89 SCSISCHH141 pKa = 6.43 LVFKK145 pKa = 9.38 SQRR148 pKa = 11.84 GLKK151 pKa = 8.35 TLYY154 pKa = 10.44 SKK156 pKa = 11.13 SLFGASASIPASRR169 pKa = 11.84 SALHH173 pKa = 6.12 VLLSSLEE180 pKa = 3.79 DD181 pKa = 2.98 RR182 pKa = 11.84 AFKK185 pKa = 9.76 NTLLRR190 pKa = 11.84 HH191 pKa = 4.88 VARR194 pKa = 11.84 AKK196 pKa = 10.39 HH197 pKa = 5.84 DD198 pKa = 3.61 RR199 pKa = 11.84 CNLLGPKK206 pKa = 8.1 SQKK209 pKa = 8.53 HH210 pKa = 3.77 TAPRR214 pKa = 11.84 PKK216 pKa = 9.77 HH217 pKa = 5.0 PKK219 pKa = 8.67 PRR221 pKa = 11.84 KK222 pKa = 8.0 CAGRR226 pKa = 11.84 SALGQTGLRR235 pKa = 11.84 TPNPRR240 pKa = 11.84 KK241 pKa = 9.78 KK242 pKa = 10.24 QPPKK246 pKa = 9.98 NRR248 pKa = 11.84 ARR250 pKa = 11.84 PRR252 pKa = 11.84 RR253 pKa = 11.84 AFPADD258 pKa = 3.1 TKK260 pKa = 10.52 QRR262 pKa = 11.84 SRR264 pKa = 11.84 ARR266 pKa = 11.84 AGSSQRR272 pKa = 11.84 IPKK275 pKa = 8.64 GHH277 pKa = 7.57 KK278 pKa = 9.21 SRR280 pKa = 11.84 KK281 pKa = 7.71 SS282 pKa = 3.17
Molecular weight: 31.68 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.317
IPC2_protein 9.794
IPC_protein 10.57
Toseland 11.082
ProMoST 10.833
Dawson 11.125
Bjellqvist 10.847
Wikipedia 11.345
Rodwell 11.389
Grimsley 11.14
Solomon 11.316
Lehninger 11.272
Nozaki 11.067
DTASelect 10.833
Thurlkill 11.052
EMBOSS 11.491
Sillero 11.067
Patrickios 11.111
IPC_peptide 11.316
IPC2_peptide 10.058
IPC2.peptide.svr19 8.675
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
82
0
82
35689
77
2505
435.2
48.71
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.094 ± 0.202
2.88 ± 0.195
4.62 ± 0.101
5.114 ± 0.157
4.363 ± 0.166
5.167 ± 0.251
2.88 ± 0.127
6.218 ± 0.277
5.548 ± 0.214
10.39 ± 0.263
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.634 ± 0.131
4.36 ± 0.142
6.136 ± 0.318
4.046 ± 0.142
4.122 ± 0.245
8.235 ± 0.281
6.901 ± 0.276
5.898 ± 0.272
1.132 ± 0.073
3.262 ± 0.119
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here