Streptomyces sp. 13-12-16

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces; unclassified Streptomyces

Average proteome isoelectric point is 6.37

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8186 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1X4HZ47|A0A1X4HZ47_9ACTN ABC transporter OS=Streptomyces sp. 13-12-16 OX=1570823 GN=B7767_29250 PE=4 SV=1
MM1 pKa = 7.32IMSVPTDD8 pKa = 3.66EE9 pKa = 5.28NDD11 pKa = 3.37LEE13 pKa = 4.28VWIDD17 pKa = 3.44QDD19 pKa = 3.99LCTGDD24 pKa = 5.01GICAQYY30 pKa = 11.17APDD33 pKa = 3.9VFEE36 pKa = 6.34LDD38 pKa = 3.44VDD40 pKa = 3.65GLAYY44 pKa = 10.79VKK46 pKa = 10.79DD47 pKa = 4.35EE48 pKa = 4.14EE49 pKa = 5.9DD50 pKa = 3.98EE51 pKa = 4.73LCTTPGAAVPVPLSLLRR68 pKa = 11.84DD69 pKa = 3.72VADD72 pKa = 3.91SAKK75 pKa = 10.46DD76 pKa = 3.65CPGDD80 pKa = 4.11CIHH83 pKa = 6.33VRR85 pKa = 11.84RR86 pKa = 11.84RR87 pKa = 11.84SDD89 pKa = 3.02RR90 pKa = 11.84VEE92 pKa = 4.07VYY94 pKa = 10.89GPDD97 pKa = 3.08AA98 pKa = 4.85

Molecular weight: 10.7 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1X4I4R4|A0A1X4I4R4_9ACTN 5-dehydro-4-deoxyglucarate dehydratase (Fragment) OS=Streptomyces sp. 13-12-16 OX=1570823 GN=B7767_18735 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILASRR35 pKa = 11.84RR36 pKa = 11.84SKK38 pKa = 10.75GRR40 pKa = 11.84ARR42 pKa = 11.84LSAA45 pKa = 3.91

Molecular weight: 5.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8186

0

8186

2361865

25

3546

288.5

30.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.124 ± 0.039

0.787 ± 0.008

6.195 ± 0.023

5.945 ± 0.028

2.749 ± 0.015

9.527 ± 0.026

2.375 ± 0.014

2.98 ± 0.017

2.092 ± 0.026

10.339 ± 0.037

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.738 ± 0.011

1.735 ± 0.016

6.06 ± 0.024

2.573 ± 0.015

8.354 ± 0.032

4.925 ± 0.018

6.23 ± 0.027

8.649 ± 0.031

1.53 ± 0.01

2.092 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski