Amino acid dipepetide frequency for Streptomyces sp. 13-12-16

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
19.542AlaAla: 19.542 ± 0.139
1.067AlaCys: 1.067 ± 0.025
8.265AlaAsp: 8.265 ± 0.069
8.764AlaGlu: 8.764 ± 0.07
3.489AlaPhe: 3.489 ± 0.04
13.014AlaGly: 13.014 ± 0.089
2.967AlaHis: 2.967 ± 0.04
3.073AlaIle: 3.073 ± 0.039
2.594AlaLys: 2.594 ± 0.048
14.36AlaLeu: 14.36 ± 0.101
2.432AlaMet: 2.432 ± 0.03
1.78AlaAsn: 1.78 ± 0.033
6.864AlaPro: 6.864 ± 0.076
3.349AlaGln: 3.349 ± 0.043
10.455AlaArg: 10.455 ± 0.079
5.616AlaSer: 5.616 ± 0.057
6.629AlaThr: 6.629 ± 0.053
12.417AlaVal: 12.417 ± 0.08
1.828AlaTrp: 1.828 ± 0.031
2.734AlaTyr: 2.734 ± 0.038
0.001AlaXaa: 0.001 ± 0.001
Cys
1.091CysAla: 1.091 ± 0.022
0.103CysCys: 0.103 ± 0.007
0.472CysAsp: 0.472 ± 0.013
0.404CysGlu: 0.404 ± 0.013
0.213CysPhe: 0.213 ± 0.01
0.961CysGly: 0.961 ± 0.022
0.208CysHis: 0.208 ± 0.01
0.145CysIle: 0.145 ± 0.008
0.098CysLys: 0.098 ± 0.006
0.742CysLeu: 0.742 ± 0.019
0.114CysMet: 0.114 ± 0.007
0.127CysAsn: 0.127 ± 0.007
0.491CysPro: 0.491 ± 0.016
0.155CysGln: 0.155 ± 0.009
0.643CysArg: 0.643 ± 0.018
0.432CysSer: 0.432 ± 0.014
0.503CysThr: 0.503 ± 0.015
0.683CysVal: 0.683 ± 0.019
0.13CysTrp: 0.13 ± 0.007
0.159CysTyr: 0.159 ± 0.008
0.0CysXaa: 0.0 ± 0.0
Asp
7.732AspAla: 7.732 ± 0.064
0.453AspCys: 0.453 ± 0.014
3.968AspAsp: 3.968 ± 0.05
4.12AspGlu: 4.12 ± 0.039
1.678AspPhe: 1.678 ± 0.032
6.892AspGly: 6.892 ± 0.07
1.545AspHis: 1.545 ± 0.026
1.878AspIle: 1.878 ± 0.033
1.16AspLys: 1.16 ± 0.026
6.309AspLeu: 6.309 ± 0.057
0.862AspMet: 0.862 ± 0.021
0.951AspAsn: 0.951 ± 0.023
4.658AspPro: 4.658 ± 0.049
1.406AspGln: 1.406 ± 0.028
5.247AspArg: 5.247 ± 0.061
2.539AspSer: 2.539 ± 0.035
3.469AspThr: 3.469 ± 0.041
4.901AspVal: 4.901 ± 0.049
1.087AspTrp: 1.087 ± 0.024
1.094AspTyr: 1.094 ± 0.025
0.0AspXaa: 0.0 ± 0.0
Glu
7.527GluAla: 7.527 ± 0.068
0.385GluCys: 0.385 ± 0.015
3.189GluAsp: 3.189 ± 0.036
3.99GluGlu: 3.99 ± 0.052
1.514GluPhe: 1.514 ± 0.027
4.61GluGly: 4.61 ± 0.043
1.639GluHis: 1.639 ± 0.023
2.213GluIle: 2.213 ± 0.035
1.561GluLys: 1.561 ± 0.032
6.922GluLeu: 6.922 ± 0.069
0.89GluMet: 0.89 ± 0.017
1.123GluAsn: 1.123 ± 0.024
3.534GluPro: 3.534 ± 0.042
2.261GluGln: 2.261 ± 0.035
5.805GluArg: 5.805 ± 0.062
2.587GluSer: 2.587 ± 0.035
3.02GluThr: 3.02 ± 0.034
4.675GluVal: 4.675 ± 0.048
0.813GluTrp: 0.813 ± 0.019
1.192GluTyr: 1.192 ± 0.021
0.0GluXaa: 0.0 ± 0.0
Phe
3.605PheAla: 3.605 ± 0.041
0.26PheCys: 0.26 ± 0.011
2.016PheAsp: 2.016 ± 0.03
1.486PheGlu: 1.486 ± 0.026
0.898PhePhe: 0.898 ± 0.022
2.965PheGly: 2.965 ± 0.037
0.649PheHis: 0.649 ± 0.016
0.651PheIle: 0.651 ± 0.017
0.502PheLys: 0.502 ± 0.017
2.678PheLeu: 2.678 ± 0.035
0.415PheMet: 0.415 ± 0.014
0.546PheAsn: 0.546 ± 0.018
1.423PhePro: 1.423 ± 0.025
0.671PheGln: 0.671 ± 0.016
1.965PheArg: 1.965 ± 0.029
1.412PheSer: 1.412 ± 0.023
2.052PheThr: 2.052 ± 0.032
2.315PheVal: 2.315 ± 0.035
0.412PheTrp: 0.412 ± 0.014
0.567PheTyr: 0.567 ± 0.016
0.0PheXaa: 0.0 ± 0.0
Gly
10.785GlyAla: 10.785 ± 0.079
0.819GlyCys: 0.819 ± 0.022
5.419GlyAsp: 5.419 ± 0.056
5.467GlyGlu: 5.467 ± 0.053
2.916GlyPhe: 2.916 ± 0.035
8.906GlyGly: 8.906 ± 0.095
2.458GlyHis: 2.458 ± 0.037
3.282GlyIle: 3.282 ± 0.04
2.311GlyLys: 2.311 ± 0.041
9.462GlyLeu: 9.462 ± 0.074
2.011GlyMet: 2.011 ± 0.03
1.746GlyAsn: 1.746 ± 0.037
5.204GlyPro: 5.204 ± 0.058
2.5GlyGln: 2.5 ± 0.038
8.301GlyArg: 8.301 ± 0.059
5.209GlySer: 5.209 ± 0.055
6.808GlyThr: 6.808 ± 0.075
7.727GlyVal: 7.727 ± 0.055
1.699GlyTrp: 1.699 ± 0.027
2.242GlyTyr: 2.242 ± 0.037
0.0GlyXaa: 0.0 ± 0.0
His
2.751HisAla: 2.751 ± 0.032
0.205HisCys: 0.205 ± 0.01
1.434HisAsp: 1.434 ± 0.027
1.296HisGlu: 1.296 ± 0.022
0.66HisPhe: 0.66 ± 0.016
2.512HisGly: 2.512 ± 0.033
0.74HisHis: 0.74 ± 0.021
0.683HisIle: 0.683 ± 0.017
0.347HisLys: 0.347 ± 0.011
2.483HisLeu: 2.483 ± 0.033
0.342HisMet: 0.342 ± 0.013
0.369HisAsn: 0.369 ± 0.013
1.857HisPro: 1.857 ± 0.031
0.632HisGln: 0.632 ± 0.016
2.316HisArg: 2.316 ± 0.036
0.989HisSer: 0.989 ± 0.021
1.427HisThr: 1.427 ± 0.025
1.814HisVal: 1.814 ± 0.028
0.397HisTrp: 0.397 ± 0.014
0.497HisTyr: 0.497 ± 0.016
0.0HisXaa: 0.0 ± 0.0
Ile
4.433IleAla: 4.433 ± 0.053
0.264IleCys: 0.264 ± 0.01
2.118IleAsp: 2.118 ± 0.03
1.941IleGlu: 1.941 ± 0.032
0.644IlePhe: 0.644 ± 0.018
3.443IleGly: 3.443 ± 0.042
0.59IleHis: 0.59 ± 0.017
0.779IleIle: 0.779 ± 0.019
0.655IleLys: 0.655 ± 0.018
2.248IleLeu: 2.248 ± 0.038
0.428IleMet: 0.428 ± 0.013
0.611IleAsn: 0.611 ± 0.02
1.611IlePro: 1.611 ± 0.024
0.663IleGln: 0.663 ± 0.019
2.213IleArg: 2.213 ± 0.038
1.522IleSer: 1.522 ± 0.022
2.17IleThr: 2.17 ± 0.031
2.623IleVal: 2.623 ± 0.036
0.36IleTrp: 0.36 ± 0.011
0.485IleTyr: 0.485 ± 0.014
0.0IleXaa: 0.0 ± 0.0
Lys
2.81LysAla: 2.81 ± 0.048
0.109LysCys: 0.109 ± 0.007
1.355LysAsp: 1.355 ± 0.032
1.278LysGlu: 1.278 ± 0.029
0.43LysPhe: 0.43 ± 0.016
1.803LysGly: 1.803 ± 0.031
0.413LysHis: 0.413 ± 0.014
0.772LysIle: 0.772 ± 0.02
0.861LysLys: 0.861 ± 0.027
1.948LysLeu: 1.948 ± 0.034
0.339LysMet: 0.339 ± 0.013
0.519LysAsn: 0.519 ± 0.021
1.25LysPro: 1.25 ± 0.025
0.666LysGln: 0.666 ± 0.017
1.442LysArg: 1.442 ± 0.025
1.109LysSer: 1.109 ± 0.025
1.221LysThr: 1.221 ± 0.031
1.865LysVal: 1.865 ± 0.032
0.273LysTrp: 0.273 ± 0.011
0.455LysTyr: 0.455 ± 0.016
0.0LysXaa: 0.0 ± 0.0
Leu
14.61LeuAla: 14.61 ± 0.1
0.843LeuCys: 0.843 ± 0.018
6.872LeuAsp: 6.872 ± 0.064
4.917LeuGlu: 4.917 ± 0.05
2.7LeuPhe: 2.7 ± 0.042
9.17LeuGly: 9.17 ± 0.077
2.368LeuHis: 2.368 ± 0.037
3.101LeuIle: 3.101 ± 0.044
2.031LeuLys: 2.031 ± 0.038
11.364LeuLeu: 11.364 ± 0.105
1.673LeuMet: 1.673 ± 0.027
1.701LeuAsn: 1.701 ± 0.028
6.475LeuPro: 6.475 ± 0.067
2.087LeuGln: 2.087 ± 0.032
8.931LeuArg: 8.931 ± 0.072
5.25LeuSer: 5.25 ± 0.054
7.137LeuThr: 7.137 ± 0.058
8.977LeuVal: 8.977 ± 0.073
1.324LeuTrp: 1.324 ± 0.025
1.858LeuTyr: 1.858 ± 0.026
0.0LeuXaa: 0.0 ± 0.0
Met
2.236MetAla: 2.236 ± 0.03
0.133MetCys: 0.133 ± 0.008
0.907MetAsp: 0.907 ± 0.021
0.786MetGlu: 0.786 ± 0.018
0.467MetPhe: 0.467 ± 0.016
1.342MetGly: 1.342 ± 0.025
0.347MetHis: 0.347 ± 0.011
0.634MetIle: 0.634 ± 0.018
0.431MetLys: 0.431 ± 0.014
1.681MetLeu: 1.681 ± 0.025
0.295MetMet: 0.295 ± 0.012
0.435MetAsn: 0.435 ± 0.012
1.093MetPro: 1.093 ± 0.024
0.429MetGln: 0.429 ± 0.014
1.452MetArg: 1.452 ± 0.024
1.31MetSer: 1.31 ± 0.025
1.566MetThr: 1.566 ± 0.028
1.283MetVal: 1.283 ± 0.028
0.226MetTrp: 0.226 ± 0.01
0.323MetTyr: 0.323 ± 0.012
0.0MetXaa: 0.0 ± 0.0
Asn
2.131AsnAla: 2.131 ± 0.032
0.174AsnCys: 0.174 ± 0.009
0.967AsnAsp: 0.967 ± 0.02
0.842AsnGlu: 0.842 ± 0.019
0.467AsnPhe: 0.467 ± 0.016
1.887AsnGly: 1.887 ± 0.033
0.418AsnHis: 0.418 ± 0.015
0.614AsnIle: 0.614 ± 0.017
0.402AsnLys: 0.402 ± 0.015
1.603AsnLeu: 1.603 ± 0.03
0.292AsnMet: 0.292 ± 0.012
0.424AsnAsn: 0.424 ± 0.014
1.323AsnPro: 1.323 ± 0.026
0.484AsnGln: 0.484 ± 0.019
1.277AsnArg: 1.277 ± 0.025
0.884AsnSer: 0.884 ± 0.022
1.092AsnThr: 1.092 ± 0.027
1.369AsnVal: 1.369 ± 0.023
0.285AsnTrp: 0.285 ± 0.012
0.417AsnTyr: 0.417 ± 0.013
0.0AsnXaa: 0.0 ± 0.0
Pro
8.374ProAla: 8.374 ± 0.075
0.349ProCys: 0.349 ± 0.012
4.716ProAsp: 4.716 ± 0.05
4.581ProGlu: 4.581 ± 0.045
1.54ProPhe: 1.54 ± 0.024
6.743ProGly: 6.743 ± 0.064
1.404ProHis: 1.404 ± 0.026
1.083ProIle: 1.083 ± 0.022
1.114ProLys: 1.114 ± 0.024
5.389ProLeu: 5.389 ± 0.055
1.036ProMet: 1.036 ± 0.02
0.837ProAsn: 0.837 ± 0.02
3.517ProPro: 3.517 ± 0.05
1.529ProGln: 1.529 ± 0.028
4.145ProArg: 4.145 ± 0.055
3.126ProSer: 3.126 ± 0.045
3.099ProThr: 3.099 ± 0.04
5.724ProVal: 5.724 ± 0.052
0.897ProTrp: 0.897 ± 0.018
1.395ProTyr: 1.395 ± 0.027
0.0ProXaa: 0.0 ± 0.0
Gln
3.442GlnAla: 3.442 ± 0.044
0.164GlnCys: 0.164 ± 0.008
1.41GlnAsp: 1.41 ± 0.024
1.475GlnGlu: 1.475 ± 0.024
0.611GlnPhe: 0.611 ± 0.017
2.168GlnGly: 2.168 ± 0.036
0.633GlnHis: 0.633 ± 0.016
0.981GlnIle: 0.981 ± 0.021
0.584GlnLys: 0.584 ± 0.017
2.714GlnLeu: 2.714 ± 0.034
0.474GlnMet: 0.474 ± 0.014
0.49GlnAsn: 0.49 ± 0.016
1.534GlnPro: 1.534 ± 0.029
1.151GlnGln: 1.151 ± 0.03
2.275GlnArg: 2.275 ± 0.031
1.14GlnSer: 1.14 ± 0.023
1.196GlnThr: 1.196 ± 0.022
2.251GlnVal: 2.251 ± 0.031
0.449GlnTrp: 0.449 ± 0.014
0.59GlnTyr: 0.59 ± 0.017
0.0GlnXaa: 0.0 ± 0.0
Arg
10.132ArgAla: 10.132 ± 0.075
0.599ArgCys: 0.599 ± 0.019
4.565ArgAsp: 4.565 ± 0.055
5.098ArgGlu: 5.098 ± 0.059
2.457ArgPhe: 2.457 ± 0.036
6.074ArgGly: 6.074 ± 0.059
2.295ArgHis: 2.295 ± 0.033
3.28ArgIle: 3.28 ± 0.04
1.66ArgLys: 1.66 ± 0.027
9.267ArgLeu: 9.267 ± 0.079
1.81ArgMet: 1.81 ± 0.029
1.393ArgAsn: 1.393 ± 0.023
5.183ArgPro: 5.183 ± 0.057
2.282ArgGln: 2.282 ± 0.031
8.141ArgArg: 8.141 ± 0.081
4.123ArgSer: 4.123 ± 0.045
5.695ArgThr: 5.695 ± 0.057
6.215ArgVal: 6.215 ± 0.056
1.418ArgTrp: 1.418 ± 0.028
1.852ArgTyr: 1.852 ± 0.031
0.0ArgXaa: 0.0 ± 0.0
Ser
6.353SerAla: 6.353 ± 0.058
0.406SerCys: 0.406 ± 0.014
2.685SerAsp: 2.685 ± 0.039
2.416SerGlu: 2.416 ± 0.034
1.492SerPhe: 1.492 ± 0.023
5.916SerGly: 5.916 ± 0.059
0.991SerHis: 0.991 ± 0.021
1.282SerIle: 1.282 ± 0.03
0.968SerLys: 0.968 ± 0.021
4.778SerLeu: 4.778 ± 0.043
0.984SerMet: 0.984 ± 0.017
0.837SerAsn: 0.837 ± 0.019
3.107SerPro: 3.107 ± 0.042
1.111SerGln: 1.111 ± 0.021
3.784SerArg: 3.784 ± 0.043
2.712SerSer: 2.712 ± 0.039
2.967SerThr: 2.967 ± 0.035
4.352SerVal: 4.352 ± 0.047
0.882SerTrp: 0.882 ± 0.02
1.224SerTyr: 1.224 ± 0.024
0.0SerXaa: 0.0 ± 0.0
Thr
8.995ThrAla: 8.995 ± 0.066
0.428ThrCys: 0.428 ± 0.014
3.892ThrAsp: 3.892 ± 0.042
3.478ThrGlu: 3.478 ± 0.039
1.601ThrPhe: 1.601 ± 0.027
7.055ThrGly: 7.055 ± 0.06
1.261ThrHis: 1.261 ± 0.026
1.563ThrIle: 1.563 ± 0.027
1.106ThrLys: 1.106 ± 0.028
5.796ThrLeu: 5.796 ± 0.051
0.945ThrMet: 0.945 ± 0.023
0.984ThrAsn: 0.984 ± 0.022
4.113ThrPro: 4.113 ± 0.05
1.24ThrGln: 1.24 ± 0.022
4.157ThrArg: 4.157 ± 0.043
3.054ThrSer: 3.054 ± 0.041
3.925ThrThr: 3.925 ± 0.057
6.381ThrVal: 6.381 ± 0.063
0.933ThrTrp: 0.933 ± 0.019
1.396ThrTyr: 1.396 ± 0.034
0.0ThrXaa: 0.0 ± 0.0
Val
10.315ValAla: 10.315 ± 0.072
0.788ValCys: 0.788 ± 0.021
5.22ValAsp: 5.22 ± 0.045
4.991ValGlu: 4.991 ± 0.053
2.559ValPhe: 2.559 ± 0.036
6.407ValGly: 6.407 ± 0.06
2.038ValHis: 2.038 ± 0.033
2.79ValIle: 2.79 ± 0.035
1.764ValLys: 1.764 ± 0.03
9.839ValLeu: 9.839 ± 0.075
1.474ValMet: 1.474 ± 0.029
1.646ValAsn: 1.646 ± 0.026
5.532ValPro: 5.532 ± 0.053
1.957ValGln: 1.957 ± 0.032
7.708ValArg: 7.708 ± 0.067
4.363ValSer: 4.363 ± 0.045
6.035ValThr: 6.035 ± 0.059
8.262ValVal: 8.262 ± 0.077
1.193ValTrp: 1.193 ± 0.024
1.611ValTyr: 1.611 ± 0.028
0.0ValXaa: 0.0 ± 0.0
Trp
1.641TrpAla: 1.641 ± 0.023
0.144TrpCys: 0.144 ± 0.008
0.857TrpAsp: 0.857 ± 0.021
0.793TrpGlu: 0.793 ± 0.021
0.517TrpPhe: 0.517 ± 0.016
1.075TrpGly: 1.075 ± 0.023
0.374TrpHis: 0.374 ± 0.014
0.537TrpIle: 0.537 ± 0.016
0.376TrpLys: 0.376 ± 0.014
1.751TrpLeu: 1.751 ± 0.03
0.298TrpMet: 0.298 ± 0.011
0.434TrpAsn: 0.434 ± 0.014
0.807TrpPro: 0.807 ± 0.018
0.593TrpGln: 0.593 ± 0.017
1.394TrpArg: 1.394 ± 0.026
0.932TrpSer: 0.932 ± 0.019
1.085TrpThr: 1.085 ± 0.024
0.976TrpVal: 0.976 ± 0.021
0.34TrpTrp: 0.34 ± 0.013
0.379TrpTyr: 0.379 ± 0.013
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.737TyrAla: 2.737 ± 0.035
0.181TyrCys: 0.181 ± 0.009
1.62TyrAsp: 1.62 ± 0.036
1.329TyrGlu: 1.329 ± 0.024
0.635TyrPhe: 0.635 ± 0.016
2.33TyrGly: 2.33 ± 0.033
0.413TyrHis: 0.413 ± 0.014
0.462TyrIle: 0.462 ± 0.015
0.39TyrLys: 0.39 ± 0.014
2.052TyrLeu: 2.052 ± 0.031
0.262TyrMet: 0.262 ± 0.01
0.4TyrAsn: 0.4 ± 0.015
1.023TyrPro: 1.023 ± 0.019
0.565TyrGln: 0.565 ± 0.017
1.885TyrArg: 1.885 ± 0.032
0.939TyrSer: 0.939 ± 0.02
1.21TyrThr: 1.21 ± 0.026
1.682TyrVal: 1.682 ± 0.029
0.355TyrTrp: 0.355 ± 0.012
0.451TyrTyr: 0.451 ± 0.016
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.001XaaAla: 0.001 ± 0.001
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.003XaaXaa: 0.003 ± 0.002
Statistics based on 8186 proteins (2361865 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski