Streptomyces phage Daudau

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Arquatrovirinae; Arequatrovirus; unclassified Arequatrovirus

Average proteome isoelectric point is 5.96

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 83 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A291LH49|A0A291LH49_9CAUD Scaffolding protein OS=Streptomyces phage Daudau OX=2041206 GN=SEA_DAUDAU_9 PE=4 SV=1
MM1 pKa = 7.75ALNDD5 pKa = 3.72AATLVIGSGNYY16 pKa = 7.79LTAPVGTDD24 pKa = 2.71IPTDD28 pKa = 3.63LLVPVSPWEE37 pKa = 4.32NVGHH41 pKa = 6.58TSLEE45 pKa = 4.57DD46 pKa = 3.02IFSISSEE53 pKa = 3.96GGEE56 pKa = 3.91ATTIGSLQNKK66 pKa = 7.29SLRR69 pKa = 11.84TKK71 pKa = 10.19YY72 pKa = 10.01SARR75 pKa = 11.84TEE77 pKa = 4.14TMAFTLQQFDD87 pKa = 3.92VKK89 pKa = 10.73GLKK92 pKa = 9.98LYY94 pKa = 10.49YY95 pKa = 10.4GSNAPTLPDD104 pKa = 3.47GSVGVPADD112 pKa = 3.63PTPTVAAFLAVFVDD126 pKa = 4.2GEE128 pKa = 4.59NVFAFYY134 pKa = 10.79APKK137 pKa = 10.81AEE139 pKa = 4.35IYY141 pKa = 10.76RR142 pKa = 11.84NDD144 pKa = 3.94DD145 pKa = 3.38LSFGDD150 pKa = 3.96TEE152 pKa = 4.59SLAGLPLGVKK162 pKa = 10.04PMAYY166 pKa = 9.89GANSWTYY173 pKa = 11.09AITPLGTVAATGATAGTPGSFTPAGATAPFDD204 pKa = 4.52LADD207 pKa = 4.96LDD209 pKa = 5.04LVTASPTTAWTTGQYY224 pKa = 10.36VVLGDD229 pKa = 3.93GSNAYY234 pKa = 8.91WDD236 pKa = 3.56GTQWVTGIAAA246 pKa = 4.22

Molecular weight: 25.46 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A291LH86|A0A291LH86_9CAUD Uncharacterized protein OS=Streptomyces phage Daudau OX=2041206 GN=SEA_DAUDAU_49 PE=4 SV=1
MM1 pKa = 7.01TPKK4 pKa = 10.16FRR6 pKa = 11.84IHH8 pKa = 7.13DD9 pKa = 3.99ANVRR13 pKa = 11.84DD14 pKa = 4.4SKK16 pKa = 11.26RR17 pKa = 11.84KK18 pKa = 10.12DD19 pKa = 3.05KK20 pKa = 10.71ATTLARR26 pKa = 11.84RR27 pKa = 11.84EE28 pKa = 3.99IRR30 pKa = 11.84HH31 pKa = 5.55QKK33 pKa = 9.93YY34 pKa = 9.06EE35 pKa = 4.39SNPAVIRR42 pKa = 11.84IAASAA47 pKa = 3.35

Molecular weight: 5.45 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

83

0

83

15476

32

1099

186.5

20.43

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.101 ± 0.327

0.788 ± 0.137

6.578 ± 0.266

7.082 ± 0.441

2.811 ± 0.197

8.407 ± 0.39

2.048 ± 0.208

4.426 ± 0.304

4.394 ± 0.294

8.155 ± 0.385

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.022 ± 0.143

2.843 ± 0.113

4.801 ± 0.178

3.463 ± 0.207

6.319 ± 0.413

5.938 ± 0.287

6.772 ± 0.282

7.101 ± 0.228

1.945 ± 0.127

3.005 ± 0.247
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski