Amino acid dipepetide frequency for Streptomyces phage Daudau

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
12.019AlaAla: 12.019 ± 0.971
0.84AlaCys: 0.84 ± 0.236
7.884AlaAsp: 7.884 ± 0.627
7.754AlaGlu: 7.754 ± 0.854
3.037AlaPhe: 3.037 ± 0.399
8.078AlaGly: 8.078 ± 0.783
2.132AlaHis: 2.132 ± 0.465
5.04AlaIle: 5.04 ± 0.54
5.428AlaLys: 5.428 ± 0.751
11.567AlaLeu: 11.567 ± 1.191
2.714AlaMet: 2.714 ± 0.382
2.585AlaAsn: 2.585 ± 0.314
4.782AlaPro: 4.782 ± 0.579
3.942AlaGln: 3.942 ± 0.559
6.333AlaArg: 6.333 ± 0.642
5.493AlaSer: 5.493 ± 0.674
7.108AlaThr: 7.108 ± 0.647
8.595AlaVal: 8.595 ± 0.756
2.456AlaTrp: 2.456 ± 0.4
3.231AlaTyr: 3.231 ± 0.475
0.0AlaXaa: 0.0 ± 0.0
Cys
0.517CysAla: 0.517 ± 0.201
0.065CysCys: 0.065 ± 0.066
0.452CysAsp: 0.452 ± 0.163
0.969CysGlu: 0.969 ± 0.372
0.194CysPhe: 0.194 ± 0.113
0.775CysGly: 0.775 ± 0.198
0.258CysHis: 0.258 ± 0.129
0.323CysIle: 0.323 ± 0.116
0.517CysLys: 0.517 ± 0.206
0.517CysLeu: 0.517 ± 0.15
0.065CysMet: 0.065 ± 0.062
0.194CysAsn: 0.194 ± 0.101
0.646CysPro: 0.646 ± 0.277
0.194CysGln: 0.194 ± 0.097
0.323CysArg: 0.323 ± 0.138
0.775CysSer: 0.775 ± 0.25
0.517CysThr: 0.517 ± 0.204
0.194CysVal: 0.194 ± 0.118
0.194CysTrp: 0.194 ± 0.109
0.194CysTyr: 0.194 ± 0.112
0.0CysXaa: 0.0 ± 0.0
Asp
7.173AspAla: 7.173 ± 0.701
0.582AspCys: 0.582 ± 0.176
5.234AspAsp: 5.234 ± 0.744
5.493AspGlu: 5.493 ± 0.701
1.68AspPhe: 1.68 ± 0.289
6.979AspGly: 6.979 ± 0.674
1.422AspHis: 1.422 ± 0.325
3.296AspIle: 3.296 ± 0.63
2.068AspLys: 2.068 ± 0.402
5.299AspLeu: 5.299 ± 0.569
1.486AspMet: 1.486 ± 0.303
1.616AspAsn: 1.616 ± 0.29
3.748AspPro: 3.748 ± 0.492
1.616AspGln: 1.616 ± 0.337
3.037AspArg: 3.037 ± 0.389
3.748AspSer: 3.748 ± 0.537
3.813AspThr: 3.813 ± 0.528
4.459AspVal: 4.459 ± 0.52
1.422AspTrp: 1.422 ± 0.299
1.616AspTyr: 1.616 ± 0.332
0.0AspXaa: 0.0 ± 0.0
Glu
9.047GluAla: 9.047 ± 0.998
1.034GluCys: 1.034 ± 0.298
4.588GluAsp: 4.588 ± 0.595
5.234GluGlu: 5.234 ± 0.842
1.68GluPhe: 1.68 ± 0.299
6.527GluGly: 6.527 ± 0.616
1.551GluHis: 1.551 ± 0.341
3.36GluIle: 3.36 ± 0.446
2.843GluLys: 2.843 ± 0.454
6.721GluLeu: 6.721 ± 0.801
1.163GluMet: 1.163 ± 0.243
1.292GluAsn: 1.292 ± 0.256
2.391GluPro: 2.391 ± 0.433
2.779GluGln: 2.779 ± 0.449
4.588GluArg: 4.588 ± 0.587
3.36GluSer: 3.36 ± 0.488
4.394GluThr: 4.394 ± 0.672
4.653GluVal: 4.653 ± 0.624
1.292GluTrp: 1.292 ± 0.306
2.326GluTyr: 2.326 ± 0.472
0.0GluXaa: 0.0 ± 0.0
Phe
2.843PheAla: 2.843 ± 0.367
0.323PheCys: 0.323 ± 0.131
2.132PheAsp: 2.132 ± 0.321
2.132PheGlu: 2.132 ± 0.328
0.388PhePhe: 0.388 ± 0.181
2.714PheGly: 2.714 ± 0.422
0.452PheHis: 0.452 ± 0.17
1.034PheIle: 1.034 ± 0.248
1.034PheLys: 1.034 ± 0.22
2.197PheLeu: 2.197 ± 0.404
0.711PheMet: 0.711 ± 0.261
1.099PheAsn: 1.099 ± 0.25
1.486PhePro: 1.486 ± 0.412
1.034PheGln: 1.034 ± 0.295
1.939PheArg: 1.939 ± 0.346
1.68PheSer: 1.68 ± 0.435
1.745PheThr: 1.745 ± 0.312
1.874PheVal: 1.874 ± 0.324
0.582PheTrp: 0.582 ± 0.176
0.711PheTyr: 0.711 ± 0.253
0.0PheXaa: 0.0 ± 0.0
Gly
7.948GlyAla: 7.948 ± 0.892
0.323GlyCys: 0.323 ± 0.179
4.911GlyAsp: 4.911 ± 0.605
5.428GlyGlu: 5.428 ± 0.515
2.52GlyPhe: 2.52 ± 0.448
6.397GlyGly: 6.397 ± 1.056
2.262GlyHis: 2.262 ± 0.41
3.877GlyIle: 3.877 ± 0.569
4.459GlyLys: 4.459 ± 0.691
6.333GlyLeu: 6.333 ± 0.616
1.551GlyMet: 1.551 ± 0.267
3.166GlyAsn: 3.166 ± 0.442
3.554GlyPro: 3.554 ± 0.469
3.166GlyGln: 3.166 ± 0.498
4.911GlyArg: 4.911 ± 0.638
6.01GlySer: 6.01 ± 0.863
6.01GlyThr: 6.01 ± 0.902
6.591GlyVal: 6.591 ± 0.693
2.326GlyTrp: 2.326 ± 0.372
2.326GlyTyr: 2.326 ± 0.373
0.0GlyXaa: 0.0 ± 0.0
His
1.486HisAla: 1.486 ± 0.372
0.065HisCys: 0.065 ± 0.06
1.034HisAsp: 1.034 ± 0.259
1.551HisGlu: 1.551 ± 0.413
0.646HisPhe: 0.646 ± 0.208
1.939HisGly: 1.939 ± 0.367
0.711HisHis: 0.711 ± 0.227
0.905HisIle: 0.905 ± 0.241
0.517HisLys: 0.517 ± 0.177
1.874HisLeu: 1.874 ± 0.299
0.323HisMet: 0.323 ± 0.125
0.323HisAsn: 0.323 ± 0.147
0.969HisPro: 0.969 ± 0.298
0.775HisGln: 0.775 ± 0.218
1.745HisArg: 1.745 ± 0.394
1.357HisSer: 1.357 ± 0.481
1.228HisThr: 1.228 ± 0.311
1.422HisVal: 1.422 ± 0.368
0.905HisTrp: 0.905 ± 0.267
0.711HisTyr: 0.711 ± 0.28
0.0HisXaa: 0.0 ± 0.0
Ile
5.105IleAla: 5.105 ± 0.495
0.323IleCys: 0.323 ± 0.17
3.554IleAsp: 3.554 ± 0.527
3.554IleGlu: 3.554 ± 0.477
1.357IlePhe: 1.357 ± 0.303
3.166IleGly: 3.166 ± 0.626
0.969IleHis: 0.969 ± 0.228
1.939IleIle: 1.939 ± 0.481
2.456IleLys: 2.456 ± 0.541
2.779IleLeu: 2.779 ± 0.472
0.388IleMet: 0.388 ± 0.141
1.034IleAsn: 1.034 ± 0.262
2.003IlePro: 2.003 ± 0.329
1.292IleGln: 1.292 ± 0.373
3.231IleArg: 3.231 ± 0.411
2.391IleSer: 2.391 ± 0.374
3.166IleThr: 3.166 ± 0.48
3.36IleVal: 3.36 ± 0.504
0.711IleTrp: 0.711 ± 0.223
1.486IleTyr: 1.486 ± 0.3
0.0IleXaa: 0.0 ± 0.0
Lys
5.687LysAla: 5.687 ± 0.621
0.388LysCys: 0.388 ± 0.145
1.874LysAsp: 1.874 ± 0.51
2.197LysGlu: 2.197 ± 0.443
0.646LysPhe: 0.646 ± 0.188
4.653LysGly: 4.653 ± 0.639
0.711LysHis: 0.711 ± 0.179
1.939LysIle: 1.939 ± 0.395
2.779LysLys: 2.779 ± 0.607
3.619LysLeu: 3.619 ± 0.563
0.775LysMet: 0.775 ± 0.215
0.84LysAsn: 0.84 ± 0.253
2.843LysPro: 2.843 ± 0.465
1.874LysGln: 1.874 ± 0.337
3.554LysArg: 3.554 ± 0.543
1.874LysSer: 1.874 ± 0.452
2.779LysThr: 2.779 ± 0.418
2.714LysVal: 2.714 ± 0.399
0.582LysTrp: 0.582 ± 0.178
1.616LysTyr: 1.616 ± 0.307
0.0LysXaa: 0.0 ± 0.0
Leu
10.856LeuAla: 10.856 ± 0.852
0.388LeuCys: 0.388 ± 0.152
4.911LeuAsp: 4.911 ± 0.606
5.428LeuGlu: 5.428 ± 0.654
1.809LeuPhe: 1.809 ± 0.383
6.527LeuGly: 6.527 ± 0.858
1.228LeuHis: 1.228 ± 0.341
3.748LeuIle: 3.748 ± 0.483
3.231LeuLys: 3.231 ± 0.543
6.01LeuLeu: 6.01 ± 0.67
2.003LeuMet: 2.003 ± 0.357
3.296LeuAsn: 3.296 ± 0.477
4.588LeuPro: 4.588 ± 0.536
2.908LeuGln: 2.908 ± 0.42
5.299LeuArg: 5.299 ± 0.746
5.428LeuSer: 5.428 ± 0.592
5.88LeuThr: 5.88 ± 0.599
5.363LeuVal: 5.363 ± 0.569
0.905LeuTrp: 0.905 ± 0.217
1.745LeuTyr: 1.745 ± 0.272
0.0LeuXaa: 0.0 ± 0.0
Met
3.36MetAla: 3.36 ± 0.528
0.065MetCys: 0.065 ± 0.071
1.228MetAsp: 1.228 ± 0.246
1.163MetGlu: 1.163 ± 0.273
0.582MetPhe: 0.582 ± 0.187
0.969MetGly: 0.969 ± 0.242
0.517MetHis: 0.517 ± 0.18
0.452MetIle: 0.452 ± 0.179
0.711MetLys: 0.711 ± 0.208
1.551MetLeu: 1.551 ± 0.331
0.258MetMet: 0.258 ± 0.139
0.388MetAsn: 0.388 ± 0.152
1.228MetPro: 1.228 ± 0.251
0.775MetGln: 0.775 ± 0.223
1.228MetArg: 1.228 ± 0.342
1.939MetSer: 1.939 ± 0.353
2.326MetThr: 2.326 ± 0.437
0.84MetVal: 0.84 ± 0.212
0.258MetTrp: 0.258 ± 0.129
0.388MetTyr: 0.388 ± 0.174
0.0MetXaa: 0.0 ± 0.0
Asn
2.843AsnAla: 2.843 ± 0.448
0.452AsnCys: 0.452 ± 0.185
1.939AsnAsp: 1.939 ± 0.357
1.616AsnGlu: 1.616 ± 0.319
1.034AsnPhe: 1.034 ± 0.323
2.779AsnGly: 2.779 ± 0.441
0.517AsnHis: 0.517 ± 0.169
1.551AsnIle: 1.551 ± 0.28
0.84AsnLys: 0.84 ± 0.187
1.874AsnLeu: 1.874 ± 0.328
0.452AsnMet: 0.452 ± 0.151
0.905AsnAsn: 0.905 ± 0.223
1.809AsnPro: 1.809 ± 0.281
1.099AsnGln: 1.099 ± 0.24
1.874AsnArg: 1.874 ± 0.305
1.551AsnSer: 1.551 ± 0.369
1.874AsnThr: 1.874 ± 0.331
2.132AsnVal: 2.132 ± 0.338
0.775AsnTrp: 0.775 ± 0.212
0.517AsnTyr: 0.517 ± 0.149
0.0AsnXaa: 0.0 ± 0.0
Pro
6.204ProAla: 6.204 ± 0.65
0.323ProCys: 0.323 ± 0.134
3.748ProAsp: 3.748 ± 0.504
4.588ProGlu: 4.588 ± 0.74
1.357ProPhe: 1.357 ± 0.317
4.459ProGly: 4.459 ± 0.413
0.517ProHis: 0.517 ± 0.165
1.809ProIle: 1.809 ± 0.456
2.456ProLys: 2.456 ± 0.462
2.973ProLeu: 2.973 ± 0.458
0.905ProMet: 0.905 ± 0.211
1.099ProAsn: 1.099 ± 0.323
2.197ProPro: 2.197 ± 0.403
1.099ProGln: 1.099 ± 0.246
2.52ProArg: 2.52 ± 0.396
2.52ProSer: 2.52 ± 0.429
3.683ProThr: 3.683 ± 0.549
3.296ProVal: 3.296 ± 0.453
0.775ProTrp: 0.775 ± 0.269
1.486ProTyr: 1.486 ± 0.247
0.0ProXaa: 0.0 ± 0.0
Gln
4.33GlnAla: 4.33 ± 0.431
0.388GlnCys: 0.388 ± 0.151
1.939GlnAsp: 1.939 ± 0.337
2.197GlnGlu: 2.197 ± 0.501
1.422GlnPhe: 1.422 ± 0.282
2.262GlnGly: 2.262 ± 0.329
0.323GlnHis: 0.323 ± 0.146
1.809GlnIle: 1.809 ± 0.302
1.357GlnLys: 1.357 ± 0.332
3.489GlnLeu: 3.489 ± 0.607
1.422GlnMet: 1.422 ± 0.368
0.646GlnAsn: 0.646 ± 0.192
1.163GlnPro: 1.163 ± 0.236
1.228GlnGln: 1.228 ± 0.293
2.456GlnArg: 2.456 ± 0.432
1.874GlnSer: 1.874 ± 0.334
2.068GlnThr: 2.068 ± 0.398
2.585GlnVal: 2.585 ± 0.358
0.582GlnTrp: 0.582 ± 0.165
1.099GlnTyr: 1.099 ± 0.249
0.0GlnXaa: 0.0 ± 0.0
Arg
5.363ArgAla: 5.363 ± 0.68
0.646ArgCys: 0.646 ± 0.178
4.394ArgAsp: 4.394 ± 0.518
4.782ArgGlu: 4.782 ± 0.713
2.456ArgPhe: 2.456 ± 0.332
4.2ArgGly: 4.2 ± 0.659
1.809ArgHis: 1.809 ± 0.326
2.262ArgIle: 2.262 ± 0.371
3.296ArgLys: 3.296 ± 0.402
4.911ArgLeu: 4.911 ± 0.566
1.68ArgMet: 1.68 ± 0.385
1.939ArgAsn: 1.939 ± 0.362
3.231ArgPro: 3.231 ± 0.442
2.456ArgGln: 2.456 ± 0.367
5.363ArgArg: 5.363 ± 0.819
3.554ArgSer: 3.554 ± 0.545
3.683ArgThr: 3.683 ± 0.501
4.006ArgVal: 4.006 ± 0.484
0.905ArgTrp: 0.905 ± 0.249
2.262ArgTyr: 2.262 ± 0.314
0.0ArgXaa: 0.0 ± 0.0
Ser
5.816SerAla: 5.816 ± 0.618
0.388SerCys: 0.388 ± 0.184
3.36SerAsp: 3.36 ± 0.498
3.037SerGlu: 3.037 ± 0.439
1.68SerPhe: 1.68 ± 0.34
5.493SerGly: 5.493 ± 0.777
1.228SerHis: 1.228 ± 0.427
2.649SerIle: 2.649 ± 0.403
2.391SerLys: 2.391 ± 0.41
5.363SerLeu: 5.363 ± 0.64
1.099SerMet: 1.099 ± 0.26
1.874SerAsn: 1.874 ± 0.337
3.102SerPro: 3.102 ± 0.411
2.132SerGln: 2.132 ± 0.362
3.489SerArg: 3.489 ± 0.598
3.554SerSer: 3.554 ± 0.426
3.877SerThr: 3.877 ± 0.544
4.911SerVal: 4.911 ± 0.746
1.68SerTrp: 1.68 ± 0.272
2.262SerTyr: 2.262 ± 0.405
0.0SerXaa: 0.0 ± 0.0
Thr
6.979ThrAla: 6.979 ± 0.658
0.388ThrCys: 0.388 ± 0.145
4.071ThrAsp: 4.071 ± 0.435
4.782ThrGlu: 4.782 ± 0.641
2.456ThrPhe: 2.456 ± 0.381
6.397ThrGly: 6.397 ± 1.029
1.292ThrHis: 1.292 ± 0.353
3.36ThrIle: 3.36 ± 0.561
2.132ThrLys: 2.132 ± 0.317
5.299ThrLeu: 5.299 ± 0.507
0.775ThrMet: 0.775 ± 0.237
1.939ThrAsn: 1.939 ± 0.385
3.489ThrPro: 3.489 ± 0.476
1.809ThrGln: 1.809 ± 0.398
3.489ThrArg: 3.489 ± 0.408
5.622ThrSer: 5.622 ± 0.8
4.588ThrThr: 4.588 ± 0.709
5.299ThrVal: 5.299 ± 0.526
1.099ThrTrp: 1.099 ± 0.278
2.456ThrTyr: 2.456 ± 0.328
0.0ThrXaa: 0.0 ± 0.0
Val
7.625ValAla: 7.625 ± 0.687
0.452ValCys: 0.452 ± 0.153
4.459ValAsp: 4.459 ± 0.458
5.234ValGlu: 5.234 ± 0.556
1.809ValPhe: 1.809 ± 0.301
5.751ValGly: 5.751 ± 0.746
1.486ValHis: 1.486 ± 0.283
3.166ValIle: 3.166 ± 0.402
3.425ValLys: 3.425 ± 0.441
5.428ValLeu: 5.428 ± 0.601
1.551ValMet: 1.551 ± 0.316
2.262ValAsn: 2.262 ± 0.402
2.908ValPro: 2.908 ± 0.395
3.102ValGln: 3.102 ± 0.495
4.136ValArg: 4.136 ± 0.437
3.877ValSer: 3.877 ± 0.426
5.363ValThr: 5.363 ± 0.573
5.557ValVal: 5.557 ± 0.649
1.228ValTrp: 1.228 ± 0.303
2.132ValTyr: 2.132 ± 0.435
0.0ValXaa: 0.0 ± 0.0
Trp
2.585TrpAla: 2.585 ± 0.388
0.129TrpCys: 0.129 ± 0.085
1.551TrpAsp: 1.551 ± 0.347
1.163TrpGlu: 1.163 ± 0.301
0.582TrpPhe: 0.582 ± 0.192
0.969TrpGly: 0.969 ± 0.257
0.323TrpHis: 0.323 ± 0.127
0.388TrpIle: 0.388 ± 0.154
0.905TrpLys: 0.905 ± 0.242
1.68TrpLeu: 1.68 ± 0.354
0.388TrpMet: 0.388 ± 0.15
1.099TrpAsn: 1.099 ± 0.239
0.646TrpPro: 0.646 ± 0.223
0.775TrpGln: 0.775 ± 0.22
1.551TrpArg: 1.551 ± 0.296
1.163TrpSer: 1.163 ± 0.237
1.745TrpThr: 1.745 ± 0.446
1.292TrpVal: 1.292 ± 0.346
0.194TrpTrp: 0.194 ± 0.119
0.323TrpTyr: 0.323 ± 0.148
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.231TyrAla: 3.231 ± 0.551
0.323TyrCys: 0.323 ± 0.205
2.52TyrAsp: 2.52 ± 0.415
2.52TyrGlu: 2.52 ± 0.394
0.775TyrPhe: 0.775 ± 0.228
3.037TyrGly: 3.037 ± 0.548
0.775TyrHis: 0.775 ± 0.274
1.357TyrIle: 1.357 ± 0.268
1.099TyrLys: 1.099 ± 0.31
2.068TyrLeu: 2.068 ± 0.386
0.452TyrMet: 0.452 ± 0.142
0.84TyrAsn: 0.84 ± 0.242
1.228TyrPro: 1.228 ± 0.265
0.582TyrGln: 0.582 ± 0.18
2.132TyrArg: 2.132 ± 0.331
1.616TyrSer: 1.616 ± 0.341
1.874TyrThr: 1.874 ± 0.336
1.874TyrVal: 1.874 ± 0.306
0.582TyrTrp: 0.582 ± 0.201
1.163TyrTyr: 1.163 ± 0.268
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 83 proteins (15476 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski