Methylocaldum marinum

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Methylococcales; Methylococcaceae; Methylocaldum

Average proteome isoelectric point is 6.64

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5413 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A250KVZ2|A0A250KVZ2_9GAMM 3-oxoacyl-ACP synthase OS=Methylocaldum marinum OX=1432792 GN=sS8_3825 PE=3 SV=1
MM1 pKa = 7.06NHH3 pKa = 7.1LFRR6 pKa = 11.84VTSIVALLTTASIANASITGDD27 pKa = 3.48VTGGGAFSDD36 pKa = 4.42DD37 pKa = 3.43VSTADD42 pKa = 3.36GWVLNNPASNGVDD55 pKa = 3.85FWTLTIDD62 pKa = 3.36QPGYY66 pKa = 10.35LSVNVGSQIDD76 pKa = 3.63FGISVYY82 pKa = 10.29QGSVSDD88 pKa = 3.78SVGFAFDD95 pKa = 3.31NAASFRR101 pKa = 11.84DD102 pKa = 4.44PITSEE107 pKa = 3.99LGTFIDD113 pKa = 3.97GTPNFGFAGSTLEE126 pKa = 4.85DD127 pKa = 3.49VFLATAGTYY136 pKa = 9.07TIAVGGSDD144 pKa = 3.98FGFSGPYY151 pKa = 8.53QYY153 pKa = 11.78EE154 pKa = 3.84MTVNVSAVPLPAAVWLFLTGLTALAGWSRR183 pKa = 11.84RR184 pKa = 11.84YY185 pKa = 7.6TAAA188 pKa = 4.05

Molecular weight: 19.54 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A250KNY6|A0A250KNY6_9GAMM Uncharacterized protein OS=Methylocaldum marinum OX=1432792 GN=sS8_1296 PE=4 SV=1
MM1 pKa = 7.42PKK3 pKa = 9.74YY4 pKa = 9.88RR5 pKa = 11.84RR6 pKa = 11.84RR7 pKa = 11.84VIDD10 pKa = 3.23GTLRR14 pKa = 11.84RR15 pKa = 11.84QIGGILRR22 pKa = 11.84EE23 pKa = 4.2LCRR26 pKa = 11.84QVGIEE31 pKa = 3.74LSEE34 pKa = 4.06RR35 pKa = 11.84HH36 pKa = 5.69TMPDD40 pKa = 3.66HH41 pKa = 6.64IHH43 pKa = 6.89PCLSIPWKK51 pKa = 10.43FSVADD56 pKa = 3.67TVVLLKK62 pKa = 10.75GKK64 pKa = 9.7SAVRR68 pKa = 11.84IHH70 pKa = 7.58RR71 pKa = 11.84EE72 pKa = 3.36FLGQKK77 pKa = 10.08RR78 pKa = 11.84NFTGLHH84 pKa = 4.99FRR86 pKa = 11.84AKK88 pKa = 9.19GFCVSTVGLDD98 pKa = 3.71GQVLRR103 pKa = 11.84AYY105 pKa = 9.38IRR107 pKa = 11.84HH108 pKa = 5.27QEE110 pKa = 3.86AEE112 pKa = 3.96EE113 pKa = 3.67RR114 pKa = 11.84RR115 pKa = 11.84IEE117 pKa = 4.09HH118 pKa = 5.32LHH120 pKa = 5.77IRR122 pKa = 11.84GLL124 pKa = 3.66

Molecular weight: 14.41 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5413

0

5413

1677097

42

6283

309.8

34.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.312 ± 0.039

1.008 ± 0.013

5.529 ± 0.029

6.392 ± 0.034

3.983 ± 0.025

7.906 ± 0.033

2.27 ± 0.017

5.213 ± 0.025

3.698 ± 0.029

10.856 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.056 ± 0.017

2.983 ± 0.02

5.166 ± 0.035

3.41 ± 0.021

7.429 ± 0.034

5.634 ± 0.027

4.992 ± 0.034

7.006 ± 0.029

1.426 ± 0.015

2.732 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski