Swingsia samuiensis
Average proteome isoelectric point is 6.69
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1984 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4Y6ULK5|A0A4Y6ULK5_9PROT Uncharacterized protein OS=Swingsia samuiensis OX=1293412 GN=E3D00_01870 PE=4 SV=1
MM1 pKa = 7.45 SYY3 pKa = 10.99 VVTEE7 pKa = 3.93 NCIKK11 pKa = 10.5 CKK13 pKa = 10.39 FMDD16 pKa = 4.29 CVEE19 pKa = 4.27 VCPVDD24 pKa = 3.86 CFYY27 pKa = 11.41 AGEE30 pKa = 4.18 NFLVINPDD38 pKa = 2.99 EE39 pKa = 5.52 CIDD42 pKa = 4.08 CGVCEE47 pKa = 4.44 PEE49 pKa = 4.91 CPAEE53 pKa = 4.95 AIFPDD58 pKa = 3.61 SDD60 pKa = 3.44 NRR62 pKa = 11.84 STPWIEE68 pKa = 3.69 INAKK72 pKa = 10.37 FSAQWPNITRR82 pKa = 11.84 KK83 pKa = 10.36 GEE85 pKa = 3.89 QPADD89 pKa = 3.36 AEE91 pKa = 4.25 EE92 pKa = 4.47 WKK94 pKa = 10.75 DD95 pKa = 3.71 KK96 pKa = 10.58 PNKK99 pKa = 10.29 AEE101 pKa = 4.2 LLSEE105 pKa = 4.43 NPHH108 pKa = 6.5 KK109 pKa = 10.82 DD110 pKa = 2.92
Molecular weight: 12.46 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.981
IPC2_protein 4.291
IPC_protein 4.19
Toseland 4.024
ProMoST 4.24
Dawson 4.139
Bjellqvist 4.342
Wikipedia 4.024
Rodwell 4.024
Grimsley 3.935
Solomon 4.139
Lehninger 4.088
Nozaki 4.253
DTASelect 4.406
Thurlkill 4.037
EMBOSS 4.037
Sillero 4.304
Patrickios 1.952
IPC_peptide 4.139
IPC2_peptide 4.291
IPC2.peptide.svr19 4.22
Protein with the highest isoelectric point:
>tr|A0A4Y6UMB2|A0A4Y6UMB2_9PROT MFS transporter OS=Swingsia samuiensis OX=1293412 GN=E3D00_00075 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.43 RR3 pKa = 11.84 TYY5 pKa = 10.27 QPSKK9 pKa = 9.73 LVRR12 pKa = 11.84 KK13 pKa = 9.15 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.54 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 TATVGGRR28 pKa = 11.84 RR29 pKa = 11.84 VLANRR34 pKa = 11.84 RR35 pKa = 11.84 TKK37 pKa = 10.41 GRR39 pKa = 11.84 KK40 pKa = 8.93 KK41 pKa = 10.59 LSAA44 pKa = 3.95
Molecular weight: 5.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.433
IPC2_protein 11.125
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.384
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.106
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.067
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1984
0
1984
654950
26
1679
330.1
36.33
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.467 ± 0.065
0.881 ± 0.013
5.282 ± 0.041
5.676 ± 0.052
3.815 ± 0.038
7.639 ± 0.067
2.567 ± 0.029
6.288 ± 0.042
4.483 ± 0.043
10.28 ± 0.061
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.366 ± 0.022
3.707 ± 0.043
5.017 ± 0.038
3.892 ± 0.033
5.879 ± 0.049
6.772 ± 0.047
5.527 ± 0.037
6.693 ± 0.056
1.318 ± 0.02
2.453 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here