Pseudomonas virus MP22

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Casadabanvirus

Average proteome isoelectric point is 6.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 51 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A7Y8R3|A7Y8R3_9CAUD Uncharacterized protein orf51 OS=Pseudomonas virus MP22 OX=397353 GN=orf51 PE=4 SV=1
MM1 pKa = 7.93RR2 pKa = 11.84LYY4 pKa = 10.51QFSRR8 pKa = 11.84GAIRR12 pKa = 11.84WSYY15 pKa = 10.82NSSDD19 pKa = 4.7RR20 pKa = 11.84DD21 pKa = 3.12ITYY24 pKa = 10.21QNQIFRR30 pKa = 11.84TVQGGITDD38 pKa = 3.5NGIICSGDD46 pKa = 3.43PQSDD50 pKa = 3.53QFVITAPADD59 pKa = 3.6LDD61 pKa = 3.68VALLYY66 pKa = 8.52KK67 pKa = 10.08TRR69 pKa = 11.84SPSDD73 pKa = 4.1AIDD76 pKa = 3.47LVVYY80 pKa = 10.54DD81 pKa = 3.99MHH83 pKa = 8.19YY84 pKa = 11.19GDD86 pKa = 5.63AEE88 pKa = 4.2AAVSWVGQIGDD99 pKa = 4.03VDD101 pKa = 4.12WPTMDD106 pKa = 3.24SCRR109 pKa = 11.84ITCVSEE115 pKa = 4.47DD116 pKa = 3.9EE117 pKa = 6.08LMDD120 pKa = 3.76QPGLIDD126 pKa = 3.86TYY128 pKa = 11.28CRR130 pKa = 11.84TCTAIVGDD138 pKa = 4.32HH139 pKa = 5.93RR140 pKa = 11.84CKK142 pKa = 11.05VNLVPYY148 pKa = 9.97RR149 pKa = 11.84VTLTPQSISGWVISSGVVAGYY170 pKa = 11.08ADD172 pKa = 3.46GWFTGGYY179 pKa = 8.66VEE181 pKa = 4.37WQVDD185 pKa = 2.96GDD187 pKa = 3.71NYY189 pKa = 11.0DD190 pKa = 3.15RR191 pKa = 11.84RR192 pKa = 11.84FIEE195 pKa = 3.78QHH197 pKa = 6.1AGADD201 pKa = 3.57LHH203 pKa = 7.71ILGGTEE209 pKa = 4.97GIPAGSQLRR218 pKa = 11.84VYY220 pKa = 9.53PGCDD224 pKa = 3.11GLAEE228 pKa = 4.32TCDD231 pKa = 3.58SKK233 pKa = 11.27FGNILNFRR241 pKa = 11.84GFNKK245 pKa = 10.37LQGRR249 pKa = 11.84SPFDD253 pKa = 3.89GDD255 pKa = 4.11QVWW258 pKa = 3.01

Molecular weight: 28.51 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A7Y8K7|A7Y8K7_9CAUD Uncharacterized protein orf18 OS=Pseudomonas virus MP22 OX=397353 GN=orf18 PE=4 SV=1
MM1 pKa = 7.2TNEE4 pKa = 4.2TKK6 pKa = 10.15TDD8 pKa = 3.35RR9 pKa = 11.84QRR11 pKa = 11.84RR12 pKa = 11.84LARR15 pKa = 11.84EE16 pKa = 3.61RR17 pKa = 11.84QRR19 pKa = 11.84AKK21 pKa = 10.65RR22 pKa = 11.84EE23 pKa = 3.43RR24 pKa = 11.84DD25 pKa = 3.22ALRR28 pKa = 11.84RR29 pKa = 11.84AALGGRR35 pKa = 11.84RR36 pKa = 11.84FNMDD40 pKa = 3.83MYY42 pKa = 10.99QGTADD47 pKa = 4.65ALDD50 pKa = 5.18LICAAGGFAEE60 pKa = 4.46PAEE63 pKa = 4.31AVTLLLHH70 pKa = 6.04NVAEE74 pKa = 4.23IAEE77 pKa = 4.21RR78 pKa = 11.84DD79 pKa = 3.33ASRR82 pKa = 11.84FAEE85 pKa = 4.92LIQKK89 pKa = 9.95RR90 pKa = 11.84SHH92 pKa = 6.71PGRR95 pKa = 11.84TKK97 pKa = 10.55RR98 pKa = 4.06

Molecular weight: 11.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

51

0

51

11683

34

1232

229.1

25.07

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.916 ± 0.86

0.77 ± 0.133

5.743 ± 0.212

6.017 ± 0.286

2.833 ± 0.228

7.626 ± 0.331

1.601 ± 0.181

4.245 ± 0.168

3.099 ± 0.27

9.783 ± 0.343

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.183 ± 0.155

3.116 ± 0.262

4.982 ± 0.396

4.87 ± 0.271

7.635 ± 0.372

5.966 ± 0.223

5.692 ± 0.271

6.522 ± 0.271

1.806 ± 0.148

2.594 ± 0.235
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski