Amino acid dipepetide frequency for Pseudomonas virus MP22

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
19.432AlaAla: 19.432 ± 4.03
1.027AlaCys: 1.027 ± 0.35
7.362AlaAsp: 7.362 ± 0.87
8.903AlaGlu: 8.903 ± 1.477
3.167AlaPhe: 3.167 ± 0.453
8.646AlaGly: 8.646 ± 1.041
1.37AlaHis: 1.37 ± 0.348
7.191AlaIle: 7.191 ± 0.723
4.366AlaLys: 4.366 ± 0.612
14.552AlaLeu: 14.552 ± 1.374
2.568AlaMet: 2.568 ± 0.473
3.595AlaAsn: 3.595 ± 1.029
5.65AlaPro: 5.65 ± 0.692
5.992AlaGln: 5.992 ± 0.909
8.988AlaArg: 8.988 ± 1.175
8.132AlaSer: 8.132 ± 1.306
6.848AlaThr: 6.848 ± 0.853
6.591AlaVal: 6.591 ± 0.897
2.397AlaTrp: 2.397 ± 0.386
2.397AlaTyr: 2.397 ± 0.467
0.0AlaXaa: 0.0 ± 0.0
Cys
0.77CysAla: 0.77 ± 0.232
0.0CysCys: 0.0 ± 0.0
0.599CysAsp: 0.599 ± 0.253
0.685CysGlu: 0.685 ± 0.279
0.514CysPhe: 0.514 ± 0.216
0.599CysGly: 0.599 ± 0.225
0.342CysHis: 0.342 ± 0.217
0.171CysIle: 0.171 ± 0.099
0.086CysLys: 0.086 ± 0.103
0.342CysLeu: 0.342 ± 0.161
0.257CysMet: 0.257 ± 0.169
0.257CysAsn: 0.257 ± 0.148
0.514CysPro: 0.514 ± 0.278
0.0CysGln: 0.0 ± 0.0
1.027CysArg: 1.027 ± 0.394
0.428CysSer: 0.428 ± 0.21
0.514CysThr: 0.514 ± 0.265
0.171CysVal: 0.171 ± 0.139
0.171CysTrp: 0.171 ± 0.123
0.257CysTyr: 0.257 ± 0.14
0.0CysXaa: 0.0 ± 0.0
Asp
8.047AspAla: 8.047 ± 1.002
0.086AspCys: 0.086 ± 0.075
2.568AspAsp: 2.568 ± 0.406
3.595AspGlu: 3.595 ± 0.598
1.626AspPhe: 1.626 ± 0.261
6.078AspGly: 6.078 ± 0.772
0.77AspHis: 0.77 ± 0.283
2.482AspIle: 2.482 ± 0.367
1.198AspLys: 1.198 ± 0.335
5.735AspLeu: 5.735 ± 0.634
1.626AspMet: 1.626 ± 0.447
1.969AspAsn: 1.969 ± 0.44
2.654AspPro: 2.654 ± 0.564
2.91AspGln: 2.91 ± 0.469
3.253AspArg: 3.253 ± 0.51
3.167AspSer: 3.167 ± 0.522
3.338AspThr: 3.338 ± 0.46
3.51AspVal: 3.51 ± 0.719
1.37AspTrp: 1.37 ± 0.319
1.455AspTyr: 1.455 ± 0.397
0.0AspXaa: 0.0 ± 0.0
Glu
7.447GluAla: 7.447 ± 0.992
0.514GluCys: 0.514 ± 0.288
2.91GluAsp: 2.91 ± 0.644
3.253GluGlu: 3.253 ± 0.465
2.054GluPhe: 2.054 ± 0.421
3.424GluGly: 3.424 ± 0.433
0.77GluHis: 0.77 ± 0.217
2.91GluIle: 2.91 ± 0.4
2.226GluLys: 2.226 ± 0.428
6.249GluLeu: 6.249 ± 0.663
1.626GluMet: 1.626 ± 0.393
1.626GluAsn: 1.626 ± 0.38
2.996GluPro: 2.996 ± 0.559
3.938GluGln: 3.938 ± 0.591
4.794GluArg: 4.794 ± 0.7
2.996GluSer: 2.996 ± 0.478
3.167GluThr: 3.167 ± 0.53
4.794GluVal: 4.794 ± 0.631
1.113GluTrp: 1.113 ± 0.295
1.37GluTyr: 1.37 ± 0.328
0.0GluXaa: 0.0 ± 0.0
Phe
4.023PheAla: 4.023 ± 0.516
0.514PheCys: 0.514 ± 0.177
2.397PheAsp: 2.397 ± 0.431
1.626PheGlu: 1.626 ± 0.463
0.77PhePhe: 0.77 ± 0.285
2.568PheGly: 2.568 ± 0.488
0.685PheHis: 0.685 ± 0.266
0.942PheIle: 0.942 ± 0.324
0.77PheLys: 0.77 ± 0.179
2.14PheLeu: 2.14 ± 0.416
0.856PheMet: 0.856 ± 0.319
0.856PheAsn: 0.856 ± 0.245
1.37PhePro: 1.37 ± 0.389
1.541PheGln: 1.541 ± 0.466
1.712PheArg: 1.712 ± 0.425
1.37PheSer: 1.37 ± 0.271
1.626PheThr: 1.626 ± 0.333
1.284PheVal: 1.284 ± 0.305
0.428PheTrp: 0.428 ± 0.187
0.856PheTyr: 0.856 ± 0.303
0.0PheXaa: 0.0 ± 0.0
Gly
7.276GlyAla: 7.276 ± 1.052
0.514GlyCys: 0.514 ± 0.208
4.109GlyAsp: 4.109 ± 0.843
4.708GlyGlu: 4.708 ± 0.576
2.739GlyPhe: 2.739 ± 0.355
6.42GlyGly: 6.42 ± 0.796
1.113GlyHis: 1.113 ± 0.25
3.253GlyIle: 3.253 ± 0.505
2.226GlyLys: 2.226 ± 0.427
8.047GlyLeu: 8.047 ± 0.84
1.712GlyMet: 1.712 ± 0.443
2.311GlyAsn: 2.311 ± 0.387
2.654GlyPro: 2.654 ± 0.43
4.28GlyGln: 4.28 ± 0.557
7.191GlyArg: 7.191 ± 0.676
4.622GlySer: 4.622 ± 0.852
4.366GlyThr: 4.366 ± 0.489
4.794GlyVal: 4.794 ± 0.56
1.798GlyTrp: 1.798 ± 0.32
2.14GlyTyr: 2.14 ± 0.518
0.0GlyXaa: 0.0 ± 0.0
His
1.626HisAla: 1.626 ± 0.299
0.086HisCys: 0.086 ± 0.081
0.77HisAsp: 0.77 ± 0.212
0.599HisGlu: 0.599 ± 0.251
0.599HisPhe: 0.599 ± 0.256
1.37HisGly: 1.37 ± 0.372
0.514HisHis: 0.514 ± 0.266
0.77HisIle: 0.77 ± 0.262
0.086HisLys: 0.086 ± 0.078
1.626HisLeu: 1.626 ± 0.39
0.856HisMet: 0.856 ± 0.294
0.942HisAsn: 0.942 ± 0.273
1.198HisPro: 1.198 ± 0.384
1.027HisGln: 1.027 ± 0.369
1.027HisArg: 1.027 ± 0.317
0.342HisSer: 0.342 ± 0.165
0.685HisThr: 0.685 ± 0.241
0.942HisVal: 0.942 ± 0.3
0.086HisTrp: 0.086 ± 0.083
0.856HisTyr: 0.856 ± 0.248
0.0HisXaa: 0.0 ± 0.0
Ile
4.879IleAla: 4.879 ± 0.747
0.428IleCys: 0.428 ± 0.207
3.424IleAsp: 3.424 ± 0.406
2.397IleGlu: 2.397 ± 0.382
0.77IlePhe: 0.77 ± 0.288
3.338IleGly: 3.338 ± 0.488
1.113IleHis: 1.113 ± 0.261
1.969IleIle: 1.969 ± 0.369
1.455IleLys: 1.455 ± 0.381
3.167IleLeu: 3.167 ± 0.457
0.428IleMet: 0.428 ± 0.188
1.198IleAsn: 1.198 ± 0.37
2.397IlePro: 2.397 ± 0.4
1.284IleGln: 1.284 ± 0.353
4.109IleArg: 4.109 ± 0.442
2.739IleSer: 2.739 ± 0.418
3.167IleThr: 3.167 ± 0.572
2.397IleVal: 2.397 ± 0.418
0.599IleTrp: 0.599 ± 0.274
1.198IleTyr: 1.198 ± 0.324
0.0IleXaa: 0.0 ± 0.0
Lys
4.109LysAla: 4.109 ± 0.649
0.0LysCys: 0.0 ± 0.0
0.942LysAsp: 0.942 ± 0.298
1.883LysGlu: 1.883 ± 0.417
0.428LysPhe: 0.428 ± 0.172
2.14LysGly: 2.14 ± 0.33
0.599LysHis: 0.599 ± 0.234
1.027LysIle: 1.027 ± 0.31
1.37LysLys: 1.37 ± 0.365
2.568LysLeu: 2.568 ± 0.623
0.856LysMet: 0.856 ± 0.25
1.113LysAsn: 1.113 ± 0.262
2.654LysPro: 2.654 ± 0.635
1.113LysGln: 1.113 ± 0.396
3.082LysArg: 3.082 ± 0.438
2.14LysSer: 2.14 ± 0.402
1.969LysThr: 1.969 ± 0.334
2.054LysVal: 2.054 ± 0.436
0.086LysTrp: 0.086 ± 0.075
0.856LysTyr: 0.856 ± 0.231
0.0LysXaa: 0.0 ± 0.0
Leu
13.611LeuAla: 13.611 ± 1.48
0.856LeuCys: 0.856 ± 0.221
6.335LeuAsp: 6.335 ± 0.752
7.276LeuGlu: 7.276 ± 0.776
2.568LeuPhe: 2.568 ± 0.572
7.619LeuGly: 7.619 ± 0.598
2.311LeuHis: 2.311 ± 0.487
3.681LeuIle: 3.681 ± 0.731
3.082LeuLys: 3.082 ± 0.577
9.416LeuLeu: 9.416 ± 0.962
1.883LeuMet: 1.883 ± 0.505
3.253LeuAsn: 3.253 ± 0.623
4.451LeuPro: 4.451 ± 0.699
4.023LeuGln: 4.023 ± 0.587
6.934LeuArg: 6.934 ± 0.822
5.136LeuSer: 5.136 ± 0.624
5.65LeuThr: 5.65 ± 1.031
6.763LeuVal: 6.763 ± 0.525
1.284LeuTrp: 1.284 ± 0.297
1.712LeuTyr: 1.712 ± 0.42
0.0LeuXaa: 0.0 ± 0.0
Met
4.023MetAla: 4.023 ± 0.492
0.086MetCys: 0.086 ± 0.091
2.311MetAsp: 2.311 ± 0.467
1.37MetGlu: 1.37 ± 0.41
0.428MetPhe: 0.428 ± 0.198
1.455MetGly: 1.455 ± 0.338
0.171MetHis: 0.171 ± 0.141
0.428MetIle: 0.428 ± 0.175
0.685MetLys: 0.685 ± 0.272
1.198MetLeu: 1.198 ± 0.284
0.257MetMet: 0.257 ± 0.143
0.599MetAsn: 0.599 ± 0.203
1.284MetPro: 1.284 ± 0.45
1.027MetGln: 1.027 ± 0.309
1.541MetArg: 1.541 ± 0.359
1.712MetSer: 1.712 ± 0.336
1.798MetThr: 1.798 ± 0.373
0.599MetVal: 0.599 ± 0.242
0.428MetTrp: 0.428 ± 0.225
0.428MetTyr: 0.428 ± 0.211
0.0MetXaa: 0.0 ± 0.0
Asn
3.681AsnAla: 3.681 ± 0.83
0.086AsnCys: 0.086 ± 0.088
1.284AsnAsp: 1.284 ± 0.344
1.37AsnGlu: 1.37 ± 0.389
0.599AsnPhe: 0.599 ± 0.314
2.91AsnGly: 2.91 ± 0.622
0.599AsnHis: 0.599 ± 0.236
1.198AsnIle: 1.198 ± 0.325
1.027AsnLys: 1.027 ± 0.31
3.51AsnLeu: 3.51 ± 0.711
0.77AsnMet: 0.77 ± 0.216
1.712AsnAsn: 1.712 ± 0.472
2.14AsnPro: 2.14 ± 0.438
1.113AsnGln: 1.113 ± 0.315
3.082AsnArg: 3.082 ± 0.406
1.626AsnSer: 1.626 ± 0.423
1.113AsnThr: 1.113 ± 0.321
1.883AsnVal: 1.883 ± 0.433
0.428AsnTrp: 0.428 ± 0.204
1.027AsnTyr: 1.027 ± 0.258
0.0AsnXaa: 0.0 ± 0.0
Pro
7.533ProAla: 7.533 ± 0.896
0.428ProCys: 0.428 ± 0.161
3.424ProAsp: 3.424 ± 0.621
2.654ProGlu: 2.654 ± 0.499
1.626ProPhe: 1.626 ± 0.366
4.451ProGly: 4.451 ± 0.5
0.599ProHis: 0.599 ± 0.245
1.541ProIle: 1.541 ± 0.294
1.712ProLys: 1.712 ± 0.378
4.109ProLeu: 4.109 ± 0.667
0.599ProMet: 0.599 ± 0.244
1.541ProAsn: 1.541 ± 0.38
2.739ProPro: 2.739 ± 0.662
2.568ProGln: 2.568 ± 0.564
3.167ProArg: 3.167 ± 0.613
3.51ProSer: 3.51 ± 0.592
2.825ProThr: 2.825 ± 0.453
2.91ProVal: 2.91 ± 0.628
0.514ProTrp: 0.514 ± 0.194
1.37ProTyr: 1.37 ± 0.365
0.0ProXaa: 0.0 ± 0.0
Gln
5.992GlnAla: 5.992 ± 1.115
0.171GlnCys: 0.171 ± 0.117
1.626GlnAsp: 1.626 ± 0.389
1.455GlnGlu: 1.455 ± 0.365
1.798GlnPhe: 1.798 ± 0.42
3.595GlnGly: 3.595 ± 0.493
1.027GlnHis: 1.027 ± 0.286
2.14GlnIle: 2.14 ± 0.416
1.37GlnLys: 1.37 ± 0.411
6.249GlnLeu: 6.249 ± 0.607
1.113GlnMet: 1.113 ± 0.373
1.626GlnAsn: 1.626 ± 0.484
1.969GlnPro: 1.969 ± 0.354
2.996GlnGln: 2.996 ± 0.732
4.879GlnArg: 4.879 ± 0.58
3.167GlnSer: 3.167 ± 0.473
1.798GlnThr: 1.798 ± 0.392
4.451GlnVal: 4.451 ± 0.707
0.685GlnTrp: 0.685 ± 0.276
0.599GlnTyr: 0.599 ± 0.262
0.0GlnXaa: 0.0 ± 0.0
Arg
9.074ArgAla: 9.074 ± 1.217
0.77ArgCys: 0.77 ± 0.297
4.28ArgAsp: 4.28 ± 0.545
5.307ArgGlu: 5.307 ± 0.664
2.568ArgPhe: 2.568 ± 0.486
3.938ArgGly: 3.938 ± 0.635
1.113ArgHis: 1.113 ± 0.233
4.109ArgIle: 4.109 ± 0.61
2.311ArgLys: 2.311 ± 0.446
7.533ArgLeu: 7.533 ± 1.081
1.284ArgMet: 1.284 ± 0.286
1.883ArgAsn: 1.883 ± 0.365
3.938ArgPro: 3.938 ± 0.849
5.051ArgGln: 5.051 ± 0.744
6.335ArgArg: 6.335 ± 0.72
4.537ArgSer: 4.537 ± 0.718
3.338ArgThr: 3.338 ± 0.409
4.965ArgVal: 4.965 ± 0.774
1.626ArgTrp: 1.626 ± 0.441
2.397ArgTyr: 2.397 ± 0.448
0.0ArgXaa: 0.0 ± 0.0
Ser
7.533SerAla: 7.533 ± 0.819
0.77SerCys: 0.77 ± 0.232
3.852SerAsp: 3.852 ± 0.668
3.253SerGlu: 3.253 ± 0.595
1.541SerPhe: 1.541 ± 0.352
4.965SerGly: 4.965 ± 0.743
0.685SerHis: 0.685 ± 0.254
2.568SerIle: 2.568 ± 0.445
2.311SerLys: 2.311 ± 0.459
5.907SerLeu: 5.907 ± 0.614
0.942SerMet: 0.942 ± 0.274
1.798SerAsn: 1.798 ± 0.375
3.766SerPro: 3.766 ± 0.651
2.397SerGln: 2.397 ± 0.338
3.082SerArg: 3.082 ± 0.508
3.852SerSer: 3.852 ± 0.475
3.766SerThr: 3.766 ± 0.725
3.253SerVal: 3.253 ± 0.528
1.798SerTrp: 1.798 ± 0.321
1.541SerTyr: 1.541 ± 0.417
0.0SerXaa: 0.0 ± 0.0
Thr
7.79ThrAla: 7.79 ± 1.158
0.342ThrCys: 0.342 ± 0.305
3.681ThrAsp: 3.681 ± 0.449
2.825ThrGlu: 2.825 ± 0.45
1.284ThrPhe: 1.284 ± 0.34
4.879ThrGly: 4.879 ± 0.688
0.685ThrHis: 0.685 ± 0.26
1.883ThrIle: 1.883 ± 0.444
1.541ThrLys: 1.541 ± 0.37
5.735ThrLeu: 5.735 ± 0.803
0.942ThrMet: 0.942 ± 0.266
1.626ThrAsn: 1.626 ± 0.321
1.798ThrPro: 1.798 ± 0.414
1.113ThrGln: 1.113 ± 0.271
3.681ThrArg: 3.681 ± 0.544
4.023ThrSer: 4.023 ± 0.684
4.28ThrThr: 4.28 ± 0.809
6.335ThrVal: 6.335 ± 0.789
1.027ThrTrp: 1.027 ± 0.295
1.455ThrTyr: 1.455 ± 0.365
0.0ThrXaa: 0.0 ± 0.0
Val
7.875ValAla: 7.875 ± 0.786
0.428ValCys: 0.428 ± 0.17
3.595ValAsp: 3.595 ± 0.493
4.537ValGlu: 4.537 ± 0.832
1.712ValPhe: 1.712 ± 0.317
4.622ValGly: 4.622 ± 0.606
0.77ValHis: 0.77 ± 0.224
2.654ValIle: 2.654 ± 0.494
2.14ValLys: 2.14 ± 0.388
5.222ValLeu: 5.222 ± 0.616
1.798ValMet: 1.798 ± 0.384
1.969ValAsn: 1.969 ± 0.307
2.996ValPro: 2.996 ± 0.359
3.766ValGln: 3.766 ± 0.54
5.051ValArg: 5.051 ± 0.565
3.167ValSer: 3.167 ± 0.639
4.708ValThr: 4.708 ± 0.636
4.023ValVal: 4.023 ± 0.691
1.027ValTrp: 1.027 ± 0.232
3.167ValTyr: 3.167 ± 0.536
0.0ValXaa: 0.0 ± 0.0
Trp
1.626TrpAla: 1.626 ± 0.338
0.257TrpCys: 0.257 ± 0.122
0.599TrpAsp: 0.599 ± 0.219
1.198TrpGlu: 1.198 ± 0.356
0.514TrpPhe: 0.514 ± 0.214
1.284TrpGly: 1.284 ± 0.398
0.171TrpHis: 0.171 ± 0.119
0.856TrpIle: 0.856 ± 0.225
0.599TrpLys: 0.599 ± 0.206
2.054TrpLeu: 2.054 ± 0.464
0.77TrpMet: 0.77 ± 0.254
0.514TrpAsn: 0.514 ± 0.192
0.856TrpPro: 0.856 ± 0.289
0.856TrpGln: 0.856 ± 0.299
1.37TrpArg: 1.37 ± 0.325
1.37TrpSer: 1.37 ± 0.357
0.685TrpThr: 0.685 ± 0.245
1.455TrpVal: 1.455 ± 0.444
0.77TrpTrp: 0.77 ± 0.233
0.257TrpTyr: 0.257 ± 0.148
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.825TyrAla: 2.825 ± 0.44
0.342TyrCys: 0.342 ± 0.183
1.37TyrAsp: 1.37 ± 0.329
1.284TyrGlu: 1.284 ± 0.357
1.027TyrPhe: 1.027 ± 0.261
1.969TyrGly: 1.969 ± 0.447
0.599TyrHis: 0.599 ± 0.343
0.685TyrIle: 0.685 ± 0.307
0.428TyrLys: 0.428 ± 0.195
2.482TyrLeu: 2.482 ± 0.392
0.599TyrMet: 0.599 ± 0.304
0.77TyrAsn: 0.77 ± 0.289
1.798TyrPro: 1.798 ± 0.487
1.712TyrGln: 1.712 ± 0.41
2.054TyrArg: 2.054 ± 0.425
1.626TyrSer: 1.626 ± 0.426
1.284TyrThr: 1.284 ± 0.349
2.054TyrVal: 2.054 ± 0.418
0.428TyrTrp: 0.428 ± 0.217
0.599TyrTyr: 0.599 ± 0.257
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 51 proteins (11683 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski