Treponema sp. OMZ 838
Average proteome isoelectric point is 6.53
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2218 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0A0X369|A0A0A0X369_9SPIO UDP-N-acetylmuramoylalanine--D-glutamate ligase OS=Treponema sp. OMZ 838 OX=1539298 GN=murD PE=3 SV=1
MM1 pKa = 7.8 AYY3 pKa = 9.38 KK4 pKa = 10.34 ISDD7 pKa = 3.41 ACVNCGACEE16 pKa = 4.32 GEE18 pKa = 4.61 CPVGAISEE26 pKa = 4.46 ANGARR31 pKa = 11.84 VIDD34 pKa = 4.02 ADD36 pKa = 3.79 ACISCGACAGVCPTEE51 pKa = 4.91 AISEE55 pKa = 4.3 EE56 pKa = 4.25
Molecular weight: 5.5 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.939
IPC2_protein 3.91
IPC_protein 3.681
Toseland 3.541
ProMoST 3.795
Dawson 3.668
Bjellqvist 3.973
Wikipedia 3.605
Rodwell 3.541
Grimsley 3.478
Solomon 3.617
Lehninger 3.567
Nozaki 3.834
DTASelect 3.897
Thurlkill 3.617
EMBOSS 3.617
Sillero 3.808
Patrickios 0.006
IPC_peptide 3.617
IPC2_peptide 3.783
IPC2.peptide.svr19 3.869
Protein with the highest isoelectric point:
>tr|A0A0A0WYY0|A0A0A0WYY0_9SPIO Tryptophanase OS=Treponema sp. OMZ 838 OX=1539298 GN=tnaA PE=3 SV=1
MM1 pKa = 7.23 FPSPFEE7 pKa = 3.87 NMRR10 pKa = 11.84 ASTASHH16 pKa = 7.03 AEE18 pKa = 4.42 NKK20 pKa = 9.91 CSTYY24 pKa = 10.19 TRR26 pKa = 11.84 YY27 pKa = 10.57 ASALFSAVSLYY38 pKa = 10.96 LLRR41 pKa = 11.84 LFSKK45 pKa = 9.55 GTVFPLPIVSGKK57 pKa = 8.88 TSGTVFLAILIVMLLADD74 pKa = 4.57 CRR76 pKa = 11.84 YY77 pKa = 9.93 LCTINTMQRR86 pKa = 11.84 YY87 pKa = 6.22 TSIGTALSAPIFSPLLFCSRR107 pKa = 11.84 GVFPLKK113 pKa = 10.09 RR114 pKa = 11.84 FKK116 pKa = 11.12 NRR118 pKa = 11.84 TCIIRR123 pKa = 11.84 AAKK126 pKa = 9.97 RR127 pKa = 11.84 IPASIAVRR135 pKa = 11.84 GRR137 pKa = 11.84 EE138 pKa = 3.88
Molecular weight: 15.27 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.343
IPC2_protein 9.794
IPC_protein 10.54
Toseland 10.511
ProMoST 10.248
Dawson 10.672
Bjellqvist 10.409
Wikipedia 10.877
Rodwell 10.906
Grimsley 10.73
Solomon 10.745
Lehninger 10.701
Nozaki 10.555
DTASelect 10.379
Thurlkill 10.54
EMBOSS 10.906
Sillero 10.599
Patrickios 10.628
IPC_peptide 10.745
IPC2_peptide 9.677
IPC2.peptide.svr19 8.35
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2218
0
2218
737495
37
3083
332.5
37.1
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.739 ± 0.06
1.255 ± 0.021
5.214 ± 0.043
6.504 ± 0.055
4.786 ± 0.043
6.635 ± 0.057
1.811 ± 0.025
7.562 ± 0.052
6.541 ± 0.047
9.468 ± 0.052
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.374 ± 0.026
4.204 ± 0.042
3.809 ± 0.033
3.512 ± 0.035
4.553 ± 0.042
6.178 ± 0.041
5.888 ± 0.046
6.279 ± 0.042
0.929 ± 0.019
3.76 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here