Cellulomonas bogoriensis 69B4 = DSM 16987
Average proteome isoelectric point is 6.15
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2898 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0A0C0Q4|A0A0A0C0Q4_9CELL Iron ABC transporter permease OS=Cellulomonas bogoriensis 69B4 = DSM 16987 OX=1386082 GN=N869_13145 PE=3 SV=1
MM1 pKa = 7.35 RR2 pKa = 11.84 TTRR5 pKa = 11.84 NRR7 pKa = 11.84 GLTAVAAGGFALALTLAACTPPPEE31 pKa = 5.15 DD32 pKa = 3.94 SPAGPGDD39 pKa = 4.22 DD40 pKa = 4.14 GDD42 pKa = 4.14 RR43 pKa = 11.84 DD44 pKa = 3.59 LAEE47 pKa = 4.17 GCEE50 pKa = 4.0 AFEE53 pKa = 4.96 QYY55 pKa = 11.37 GDD57 pKa = 3.72 LSGTSVSVYY66 pKa = 10.12 TSITAPEE73 pKa = 4.44 DD74 pKa = 3.42 QPHH77 pKa = 6.5 IDD79 pKa = 3.46 SYY81 pKa = 11.37 TEE83 pKa = 3.7 FEE85 pKa = 4.54 RR86 pKa = 11.84 CTGADD91 pKa = 2.59 IVYY94 pKa = 10.2 DD95 pKa = 3.72 GSRR98 pKa = 11.84 EE99 pKa = 3.9 FEE101 pKa = 3.89 AQLPVRR107 pKa = 11.84 VQAGNPPDD115 pKa = 3.36 IAYY118 pKa = 9.43 IPQPGLLQTIVRR130 pKa = 11.84 DD131 pKa = 4.05 FPDD134 pKa = 3.15 AVVPVGDD141 pKa = 3.84 LAEE144 pKa = 4.66 SNVDD148 pKa = 3.42 EE149 pKa = 5.2 YY150 pKa = 11.9 YY151 pKa = 10.83 DD152 pKa = 4.3 PSWKK156 pKa = 10.32 EE157 pKa = 3.59 YY158 pKa = 8.79 GTVDD162 pKa = 3.04 GTYY165 pKa = 10.15 YY166 pKa = 9.98 ATPLGANVKK175 pKa = 10.15 SFVWYY180 pKa = 10.67 SPDD183 pKa = 3.03 AFADD187 pKa = 3.26 AGYY190 pKa = 9.23 EE191 pKa = 4.2 IPEE194 pKa = 4.18 TWDD197 pKa = 3.14 EE198 pKa = 4.4 LLALSDD204 pKa = 4.58 QIVDD208 pKa = 3.94 DD209 pKa = 6.05 GGTPWCVGIEE219 pKa = 4.16 SGDD222 pKa = 3.55 ATGWPATDD230 pKa = 3.1 WLEE233 pKa = 4.49 DD234 pKa = 3.53 VMLRR238 pKa = 11.84 TAGPEE243 pKa = 4.35 VYY245 pKa = 10.05 DD246 pKa = 3.02 QWVDD250 pKa = 3.35 HH251 pKa = 6.95 EE252 pKa = 5.18 IPFNDD257 pKa = 3.82 PAVAEE262 pKa = 4.07 ALAAVGEE269 pKa = 4.2 ILKK272 pKa = 10.84 NEE274 pKa = 4.46 DD275 pKa = 3.22 YY276 pKa = 11.63 VNAGFGDD283 pKa = 3.74 VRR285 pKa = 11.84 SIASTPFTDD294 pKa = 3.93 AGFPILDD301 pKa = 3.99 GEE303 pKa = 4.67 CWMHH307 pKa = 6.24 RR308 pKa = 11.84 QASFYY313 pKa = 9.87 AANWEE318 pKa = 4.31 SADD321 pKa = 3.58 PDD323 pKa = 3.88 VQVGEE328 pKa = 4.39 NGDD331 pKa = 3.52 VFAFYY336 pKa = 10.94 LPGATTDD343 pKa = 3.52 EE344 pKa = 4.46 SPLLGGGEE352 pKa = 3.98 FVAAFDD358 pKa = 4.56 EE359 pKa = 4.72 RR360 pKa = 11.84 PEE362 pKa = 4.05 VQAFQAFLTSPEE374 pKa = 3.95 WVNAKK379 pKa = 9.96 IPEE382 pKa = 4.47 HH383 pKa = 7.17 PDD385 pKa = 3.48 GGWVSANRR393 pKa = 11.84 GQDD396 pKa = 3.25 MEE398 pKa = 5.72 LYY400 pKa = 10.42 ASPIDD405 pKa = 3.74 RR406 pKa = 11.84 LSAEE410 pKa = 4.59 LLADD414 pKa = 4.28 DD415 pKa = 5.12 DD416 pKa = 4.42 AVFRR420 pKa = 11.84 FDD422 pKa = 5.94 ASDD425 pKa = 3.65 LMPGEE430 pKa = 4.43 VGSNSFWTEE439 pKa = 3.5 MVNWIANDD447 pKa = 3.85 KK448 pKa = 10.21 PDD450 pKa = 4.48 QDD452 pKa = 3.88 VLDD455 pKa = 5.65 DD456 pKa = 5.88 IEE458 pKa = 5.06 ASWPP462 pKa = 3.5
Molecular weight: 50.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.712
IPC2_protein 3.605
IPC_protein 3.643
Toseland 3.414
ProMoST 3.795
Dawson 3.63
Bjellqvist 3.783
Wikipedia 3.567
Rodwell 3.465
Grimsley 3.325
Solomon 3.63
Lehninger 3.579
Nozaki 3.745
DTASelect 3.986
Thurlkill 3.465
EMBOSS 3.567
Sillero 3.757
Patrickios 1.341
IPC_peptide 3.617
IPC2_peptide 3.732
IPC2.peptide.svr19 3.716
Protein with the highest isoelectric point:
>tr|A0A0A0BYF2|A0A0A0BYF2_9CELL Uncharacterized protein OS=Cellulomonas bogoriensis 69B4 = DSM 16987 OX=1386082 GN=N869_14270 PE=4 SV=1
MM1 pKa = 7.7 GGPPPPRR8 pKa = 11.84 RR9 pKa = 11.84 PGRR12 pKa = 11.84 PSAGRR17 pKa = 11.84 ARR19 pKa = 11.84 PGRR22 pKa = 11.84 RR23 pKa = 11.84 GPGPPRR29 pKa = 11.84 PPARR33 pKa = 11.84 AAQRR37 pKa = 11.84 AARR40 pKa = 11.84 VPAAPPRR47 pKa = 11.84 APRR50 pKa = 11.84 TGPRR54 pKa = 11.84 RR55 pKa = 11.84 RR56 pKa = 11.84 GRR58 pKa = 11.84 RR59 pKa = 11.84 RR60 pKa = 11.84 ARR62 pKa = 11.84 PDD64 pKa = 2.75 VTRR67 pKa = 11.84 PARR70 pKa = 11.84 SGG72 pKa = 3.21
Molecular weight: 7.67 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.52
IPC2_protein 11.228
IPC_protein 12.837
Toseland 12.998
ProMoST 13.495
Dawson 12.998
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.501
Grimsley 13.042
Solomon 13.495
Lehninger 13.393
Nozaki 12.998
DTASelect 12.998
Thurlkill 12.998
EMBOSS 13.495
Sillero 12.998
Patrickios 12.223
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.217
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2898
0
2898
906499
37
2055
312.8
33.35
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.225 ± 0.056
0.621 ± 0.012
6.423 ± 0.039
5.569 ± 0.042
2.464 ± 0.027
9.529 ± 0.042
2.351 ± 0.028
2.858 ± 0.033
1.299 ± 0.025
10.34 ± 0.045
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.784 ± 0.02
1.504 ± 0.021
5.996 ± 0.035
2.929 ± 0.023
8.028 ± 0.052
4.749 ± 0.033
6.554 ± 0.038
10.49 ± 0.051
1.51 ± 0.019
1.776 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here