Felis catus gammaherpesvirus 1
Average proteome isoelectric point is 6.88
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 87 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0M3T960|A0A0M3T960_9GAMA DNA polymerase OS=Felis catus gammaherpesvirus 1 OX=1452540 PE=3 SV=1
MM1 pKa = 7.44 NSKK4 pKa = 8.78 CTEE7 pKa = 3.61 IVLVFLEE14 pKa = 4.9 LNNPSYY20 pKa = 10.83 PVTVLINITLSFPGLHH36 pKa = 6.77 LYY38 pKa = 9.92 IEE40 pKa = 4.31 NSGLYY45 pKa = 8.56 KK46 pKa = 9.53 TVCKK50 pKa = 10.37 NLEE53 pKa = 4.29 FIRR56 pKa = 11.84 GLLAMAYY63 pKa = 9.55 YY64 pKa = 10.04 GRR66 pKa = 11.84 SVQTANSTCIPVEE79 pKa = 4.34 TTWPPEE85 pKa = 3.6 EE86 pKa = 4.48 DD87 pKa = 3.6 TPNGPEE93 pKa = 3.89 EE94 pKa = 5.18 DD95 pKa = 3.34 EE96 pKa = 4.24 WPYY99 pKa = 11.72 GDD101 pKa = 4.55 IIGGGDD107 pKa = 3.44 VDD109 pKa = 4.8 SGGLINQPVEE119 pKa = 4.53 DD120 pKa = 4.62 EE121 pKa = 4.18 WPKK124 pKa = 10.76 PLDD127 pKa = 3.39 AVGPNQPEE135 pKa = 3.98 EE136 pKa = 4.44 SDD138 pKa = 3.38 WPGDD142 pKa = 3.57 LTT144 pKa = 4.82
Molecular weight: 15.87 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.791
IPC2_protein 3.897
IPC_protein 3.821
Toseland 3.643
ProMoST 3.961
Dawson 3.783
Bjellqvist 3.948
Wikipedia 3.681
Rodwell 3.656
Grimsley 3.554
Solomon 3.77
Lehninger 3.732
Nozaki 3.91
DTASelect 4.05
Thurlkill 3.681
EMBOSS 3.694
Sillero 3.935
Patrickios 0.604
IPC_peptide 3.77
IPC2_peptide 3.91
IPC2.peptide.svr19 3.833
Protein with the highest isoelectric point:
>tr|A0A0M4MD83|A0A0M4MD83_9GAMA F7 OS=Felis catus gammaherpesvirus 1 OX=1452540 PE=4 SV=1
MM1 pKa = 7.45 IAGASIWLHH10 pKa = 4.98 VLLINILGKK19 pKa = 8.14 TVHH22 pKa = 6.6 ASSSVITSSEE32 pKa = 3.94 TTKK35 pKa = 10.84 AVPTTVTVTSVTSGLGSSLTSQNHH59 pKa = 3.84 QTNTVTTVNFNSQLLKK75 pKa = 10.3 STDD78 pKa = 3.45 LVMVMLLVTIAGLFLLIMSGVIITDD103 pKa = 3.77 CYY105 pKa = 9.89 KK106 pKa = 10.5 NRR108 pKa = 11.84 NRR110 pKa = 11.84 RR111 pKa = 11.84 YY112 pKa = 9.74 QRR114 pKa = 11.84 VPLIPLRR121 pKa = 11.84 NYY123 pKa = 8.62 PNQRR127 pKa = 11.84 NRR129 pKa = 11.84 MWGNN133 pKa = 2.98
Molecular weight: 14.62 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.327
IPC2_protein 9.984
IPC_protein 10.833
Toseland 10.804
ProMoST 10.555
Dawson 10.906
Bjellqvist 10.657
Wikipedia 11.14
Rodwell 11.125
Grimsley 10.965
Solomon 11.038
Lehninger 11.008
Nozaki 10.789
DTASelect 10.643
Thurlkill 10.804
EMBOSS 11.213
Sillero 10.847
Patrickios 10.906
IPC_peptide 11.052
IPC2_peptide 9.721
IPC2.peptide.svr19 8.222
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
87
0
87
36473
55
2259
419.2
47.52
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.68 ± 0.156
2.506 ± 0.15
4.401 ± 0.176
5.045 ± 0.234
5.113 ± 0.179
4.59 ± 0.263
2.72 ± 0.094
7.241 ± 0.271
6.27 ± 0.237
10.276 ± 0.268
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.196 ± 0.102
5.711 ± 0.199
5.168 ± 0.356
4.532 ± 0.159
3.356 ± 0.19
8.283 ± 0.207
7.002 ± 0.243
5.854 ± 0.192
1.195 ± 0.111
3.86 ± 0.15
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here