Streptococcus phage Javan244
Average proteome isoelectric point is 6.07
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 36 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D6AW31|A0A4D6AW31_9CAUD Uncharacterized protein OS=Streptococcus phage Javan244 OX=2548067 GN=Javan244_0032 PE=4 SV=1
MM1 pKa = 7.57 LGTVYY6 pKa = 10.6 GHH8 pKa = 7.73 DD9 pKa = 4.19 DD10 pKa = 3.5 LASVLVHH17 pKa = 6.56 WDD19 pKa = 3.28 NGSSLSVVYY28 pKa = 10.23 GVDD31 pKa = 3.27 RR32 pKa = 11.84 ITRR35 pKa = 11.84 VEE37 pKa = 3.77
Molecular weight: 4.06 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.636
IPC2_protein 4.889
IPC_protein 4.546
Toseland 4.406
ProMoST 4.482
Dawson 4.584
Bjellqvist 4.902
Wikipedia 4.558
Rodwell 4.431
Grimsley 4.342
Solomon 4.558
Lehninger 4.52
Nozaki 4.724
DTASelect 4.978
Thurlkill 4.495
EMBOSS 4.584
Sillero 4.724
Patrickios 3.503
IPC_peptide 4.558
IPC2_peptide 4.698
IPC2.peptide.svr19 4.739
Protein with the highest isoelectric point:
>tr|A0A4D6AV54|A0A4D6AV54_9CAUD DNA helicase OS=Streptococcus phage Javan244 OX=2548067 GN=Javan244_0028 PE=4 SV=1
MM1 pKa = 7.65 RR2 pKa = 11.84 ADD4 pKa = 3.58 EE5 pKa = 4.32 LSRR8 pKa = 11.84 AIEE11 pKa = 4.25 KK12 pKa = 10.69 KK13 pKa = 10.46 LEE15 pKa = 4.29 EE16 pKa = 4.35 YY17 pKa = 10.53 LHH19 pKa = 5.5 QTTEE23 pKa = 4.43 TVVEE27 pKa = 4.15 AVDD30 pKa = 3.59 AVTDD34 pKa = 3.71 EE35 pKa = 5.41 AIATLKK41 pKa = 9.42 STSPKK46 pKa = 9.6 RR47 pKa = 11.84 SGRR50 pKa = 11.84 YY51 pKa = 6.83 VRR53 pKa = 11.84 GWTSKK58 pKa = 10.41 EE59 pKa = 3.71 MSSTSTGKK67 pKa = 8.57 TKK69 pKa = 10.18 TIHH72 pKa = 5.97 NRR74 pKa = 11.84 TPGLTHH80 pKa = 7.19 LLEE83 pKa = 4.53 NGYY86 pKa = 9.75 IKK88 pKa = 10.8 QNGGRR93 pKa = 11.84 VSGRR97 pKa = 11.84 PHH99 pKa = 7.06 IGPVEE104 pKa = 4.11 QKK106 pKa = 10.6 AVQALEE112 pKa = 3.53 KK113 pKa = 10.31 RR114 pKa = 11.84 IRR116 pKa = 11.84 GKK118 pKa = 10.61 LL119 pKa = 3.37
Molecular weight: 13.21 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.027
IPC2_protein 9.194
IPC_protein 9.194
Toseland 10.131
ProMoST 9.677
Dawson 10.262
Bjellqvist 9.867
Wikipedia 10.394
Rodwell 10.804
Grimsley 10.306
Solomon 10.306
Lehninger 10.277
Nozaki 10.087
DTASelect 9.867
Thurlkill 10.131
EMBOSS 10.511
Sillero 10.175
Patrickios 10.54
IPC_peptide 10.306
IPC2_peptide 8.258
IPC2.peptide.svr19 8.26
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
36
0
36
10845
37
1039
301.3
33.8
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.796 ± 0.512
0.83 ± 0.121
5.966 ± 0.36
7.054 ± 0.315
3.744 ± 0.183
6.943 ± 0.389
1.835 ± 0.181
6.27 ± 0.351
7.091 ± 0.32
8.944 ± 0.309
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.425 ± 0.145
4.62 ± 0.231
3.006 ± 0.193
4.343 ± 0.215
4.546 ± 0.347
7.063 ± 0.401
6.777 ± 0.424
6.814 ± 0.228
1.429 ± 0.128
3.504 ± 0.3
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here