Anaerocolumna sp. CBA3638

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; Anaerocolumna

Average proteome isoelectric point is 6.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4505 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6P1TV38|A0A6P1TV38_9FIRM Sigma-70 family RNA polymerase sigma factor OS=Anaerocolumna sp. CBA3638 OX=2696063 GN=Ana3638_01180 PE=3 SV=1
MM1 pKa = 7.02EE2 pKa = 4.44TQTFLSAGTTFVSSTLAPYY21 pKa = 10.28NLSTSAVIAVGTHH34 pKa = 5.93PVYY37 pKa = 10.45QDD39 pKa = 3.44SISFLRR45 pKa = 11.84FDD47 pKa = 4.71LSSISAANASSAVLRR62 pKa = 11.84LFVFEE67 pKa = 5.09KK68 pKa = 10.56EE69 pKa = 4.22GAAPSPVVINRR80 pKa = 11.84VTSDD84 pKa = 3.29YY85 pKa = 11.24DD86 pKa = 3.57INTVTYY92 pKa = 7.47NTRR95 pKa = 11.84PAYY98 pKa = 10.6VNTEE102 pKa = 3.65ITSEE106 pKa = 4.07VSSDD110 pKa = 3.39EE111 pKa = 4.11VIKK114 pKa = 10.92YY115 pKa = 10.1IEE117 pKa = 4.38IPVTTLVNQWLNGDD131 pKa = 3.97FPNYY135 pKa = 9.82GFALTNPDD143 pKa = 2.87GTTSVLFGGKK153 pKa = 8.63PVGASYY159 pKa = 10.52EE160 pKa = 4.07PQLVITYY167 pKa = 10.42SSEE170 pKa = 4.31TPGKK174 pKa = 8.93LTGIQAQLQRR184 pKa = 11.84SPGTIIADD192 pKa = 3.74GANVIFDD199 pKa = 3.95STLTDD204 pKa = 3.32QSEE207 pKa = 4.71SISYY211 pKa = 10.49NPATGEE217 pKa = 4.05FTISKK222 pKa = 8.85PDD224 pKa = 3.14NYY226 pKa = 10.56YY227 pKa = 10.01ISWWVTTDD235 pKa = 3.33GSAGPVNMVFGLVADD250 pKa = 4.5GTTIAVGNSPDD261 pKa = 3.16VTGQVDD267 pKa = 3.69GDD269 pKa = 4.01GFLTVSNDD277 pKa = 3.16PVTLTLVNQTGADD290 pKa = 3.31VLYY293 pKa = 11.25ANIPVQANISIITVVSDD310 pKa = 3.35SSVV313 pKa = 2.62

Molecular weight: 33.12 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6P1THG1|A0A6P1THG1_9FIRM Uncharacterized protein OS=Anaerocolumna sp. CBA3638 OX=2696063 GN=Ana3638_02850 PE=4 SV=1
MM1 pKa = 7.67KK2 pKa = 8.72MTFQPKK8 pKa = 8.86KK9 pKa = 8.2RR10 pKa = 11.84SRR12 pKa = 11.84AKK14 pKa = 9.27VHH16 pKa = 5.81GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTSNGRR28 pKa = 11.84KK29 pKa = 8.69VLASRR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.26GRR39 pKa = 11.84HH40 pKa = 5.5KK41 pKa = 10.9LSAA44 pKa = 3.8

Molecular weight: 5.06 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4505

0

4505

1407776

29

1706

312.5

35.2

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.442 ± 0.037

1.251 ± 0.013

5.518 ± 0.028

7.011 ± 0.041

4.36 ± 0.028

6.626 ± 0.034

1.601 ± 0.016

8.838 ± 0.04

7.485 ± 0.033

9.233 ± 0.037

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.806 ± 0.019

5.56 ± 0.029

3.205 ± 0.023

2.967 ± 0.019

3.638 ± 0.021

6.165 ± 0.033

5.631 ± 0.032

6.313 ± 0.028

0.907 ± 0.012

4.444 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski