Amino acid dipepetide frequency for Anaerocolumna sp. CBA3638

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	5.705AlaAla: 5.705 ± 0.086	0.864AlaCys: 0.864 ± 0.025	3.805AlaAsp: 3.805 ± 0.05	4.32AlaGlu: 4.32 ± 0.061	2.853AlaPhe: 2.853 ± 0.045	5.091AlaGly: 5.091 ± 0.075	0.872AlaHis: 0.872 ± 0.024	5.183AlaIle: 5.183 ± 0.062	4.549AlaLys: 4.549 ± 0.054	6.071AlaLeu: 6.071 ± 0.068	1.892AlaMet: 1.892 ± 0.04	2.72AlaAsn: 2.72 ± 0.043	1.729AlaPro: 1.729 ± 0.038	1.58AlaGln: 1.58 ± 0.037	2.233AlaArg: 2.233 ± 0.043	3.648AlaSer: 3.648 ± 0.053	3.02AlaThr: 3.02 ± 0.053	5.185AlaVal: 5.185 ± 0.063	0.506AlaTrp: 0.506 ± 0.019	2.596AlaTyr: 2.596 ± 0.041	0.0AlaXaa: 0.0 ± 0.0
Cys	0.734CysAla: 0.734 ± 0.024	0.211CysCys: 0.211 ± 0.012	0.719CysAsp: 0.719 ± 0.021	0.742CysGlu: 0.742 ± 0.022	0.58CysPhe: 0.58 ± 0.025	1.21CysGly: 1.21 ± 0.031	0.257CysHis: 0.257 ± 0.014	1.151CysIle: 1.151 ± 0.028	0.808CysLys: 0.808 ± 0.022	1.005CysLeu: 1.005 ± 0.024	0.308CysMet: 0.308 ± 0.013	0.638CysAsn: 0.638 ± 0.023	0.566CysPro: 0.566 ± 0.029	0.313CysGln: 0.313 ± 0.015	0.486CysArg: 0.486 ± 0.019	0.82CysSer: 0.82 ± 0.023	0.666CysThr: 0.666 ± 0.019	0.65CysVal: 0.65 ± 0.02	0.109CysTrp: 0.109 ± 0.008	0.536CysTyr: 0.536 ± 0.021	0.0CysXaa: 0.0 ± 0.0
Asp	3.142AspAla: 3.142 ± 0.053	0.641AspCys: 0.641 ± 0.022	2.556AspAsp: 2.556 ± 0.045	4.046AspGlu: 4.046 ± 0.056	2.721AspPhe: 2.721 ± 0.049	3.714AspGly: 3.714 ± 0.063	0.716AspHis: 0.716 ± 0.022	5.473AspIle: 5.473 ± 0.059	4.457AspLys: 4.457 ± 0.06	4.607AspLeu: 4.607 ± 0.061	1.714AspMet: 1.714 ± 0.035	3.132AspAsn: 3.132 ± 0.057	1.536AspPro: 1.536 ± 0.038	1.274AspGln: 1.274 ± 0.028	1.975AspArg: 1.975 ± 0.043	3.275AspSer: 3.275 ± 0.046	3.282AspThr: 3.282 ± 0.061	3.28AspVal: 3.28 ± 0.058	0.587AspTrp: 0.587 ± 0.021	3.053AspTyr: 3.053 ± 0.045	0.0AspXaa: 0.0 ± 0.0
Glu	4.656GluAla: 4.656 ± 0.063	0.707GluCys: 0.707 ± 0.025	3.894GluAsp: 3.894 ± 0.058	6.304GluGlu: 6.304 ± 0.083	2.743GluPhe: 2.743 ± 0.045	3.655GluGly: 3.655 ± 0.054	1.106GluHis: 1.106 ± 0.032	6.178GluIle: 6.178 ± 0.074	5.936GluLys: 5.936 ± 0.066	6.701GluLeu: 6.701 ± 0.083	1.953GluMet: 1.953 ± 0.035	4.248GluAsn: 4.248 ± 0.064	1.737GluPro: 1.737 ± 0.036	2.498GluGln: 2.498 ± 0.046	2.657GluArg: 2.657 ± 0.051	3.413GluSer: 3.413 ± 0.048	3.56GluThr: 3.56 ± 0.046	4.308GluVal: 4.308 ± 0.053	0.619GluTrp: 0.619 ± 0.023	3.239GluTyr: 3.239 ± 0.052	0.0GluXaa: 0.0 ± 0.0
Phe	2.59PheAla: 2.59 ± 0.043	0.592PheCys: 0.592 ± 0.021	2.602PheAsp: 2.602 ± 0.045	2.633PheGlu: 2.633 ± 0.043	1.918PhePhe: 1.918 ± 0.042	2.983PheGly: 2.983 ± 0.054	0.881PheHis: 0.881 ± 0.027	4.03PheIle: 4.03 ± 0.056	2.799PheLys: 2.799 ± 0.046	4.07PheLeu: 4.07 ± 0.062	1.229PheMet: 1.229 ± 0.033	2.277PheAsn: 2.277 ± 0.04	1.369PhePro: 1.369 ± 0.032	1.336PheGln: 1.336 ± 0.029	1.548PheArg: 1.548 ± 0.034	3.001PheSer: 3.001 ± 0.05	2.723PheThr: 2.723 ± 0.046	2.552PheVal: 2.552 ± 0.042	0.455PheTrp: 0.455 ± 0.019	2.012PheTyr: 2.012 ± 0.033	0.0PheXaa: 0.0 ± 0.0
Gly	4.091GlyAla: 4.091 ± 0.062	1.075GlyCys: 1.075 ± 0.031	3.278GlyAsp: 3.278 ± 0.051	4.158GlyGlu: 4.158 ± 0.06	3.204GlyPhe: 3.204 ± 0.054	4.323GlyGly: 4.323 ± 0.069	1.067GlyHis: 1.067 ± 0.03	6.817GlyIle: 6.817 ± 0.073	5.218GlyLys: 5.218 ± 0.07	5.876GlyLeu: 5.876 ± 0.068	2.037GlyMet: 2.037 ± 0.042	3.468GlyAsn: 3.468 ± 0.052	1.25GlyPro: 1.25 ± 0.065	1.785GlyGln: 1.785 ± 0.04	2.417GlyArg: 2.417 ± 0.045	3.877GlySer: 3.877 ± 0.056	3.984GlyThr: 3.984 ± 0.064	4.381GlyVal: 4.381 ± 0.066	0.645GlyTrp: 0.645 ± 0.023	3.311GlyTyr: 3.311 ± 0.047	0.0GlyXaa: 0.0 ± 0.0
His	0.842HisAla: 0.842 ± 0.022	0.253HisCys: 0.253 ± 0.013	0.791HisAsp: 0.791 ± 0.023	0.899HisGlu: 0.899 ± 0.025	0.81HisPhe: 0.81 ± 0.027	1.116HisGly: 1.116 ± 0.028	0.34HisHis: 0.34 ± 0.015	1.558HisIle: 1.558 ± 0.032	1.023HisLys: 1.023 ± 0.029	1.423HisLeu: 1.423 ± 0.029	0.472HisMet: 0.472 ± 0.018	0.918HisAsn: 0.918 ± 0.026	0.738HisPro: 0.738 ± 0.024	0.466HisGln: 0.466 ± 0.02	0.632HisArg: 0.632 ± 0.02	0.96HisSer: 0.96 ± 0.025	0.86HisThr: 0.86 ± 0.022	0.931HisVal: 0.931 ± 0.025	0.185HisTrp: 0.185 ± 0.011	0.787HisTyr: 0.787 ± 0.026	0.0HisXaa: 0.0 ± 0.0
Ile	5.779IleAla: 5.779 ± 0.072	1.216IleCys: 1.216 ± 0.034	4.648IleAsp: 4.648 ± 0.07	5.22IleGlu: 5.22 ± 0.073	3.624IlePhe: 3.624 ± 0.058	5.769IleGly: 5.769 ± 0.075	1.528IleHis: 1.528 ± 0.036	7.787IleIle: 7.787 ± 0.082	6.329IleLys: 6.329 ± 0.073	8.482IleLeu: 8.482 ± 0.093	2.308IleMet: 2.308 ± 0.047	5.072IleAsn: 5.072 ± 0.063	3.643IlePro: 3.643 ± 0.056	2.598IleGln: 2.598 ± 0.044	3.444IleArg: 3.444 ± 0.057	6.11IleSer: 6.11 ± 0.066	5.681IleThr: 5.681 ± 0.065	5.004IleVal: 5.004 ± 0.06	0.703IleTrp: 0.703 ± 0.022	3.438IleTyr: 3.438 ± 0.052	0.0IleXaa: 0.0 ± 0.0
Lys	4.96LysAla: 4.96 ± 0.06	0.665LysCys: 0.665 ± 0.024	4.526LysAsp: 4.526 ± 0.062	7.146LysGlu: 7.146 ± 0.092	2.304LysPhe: 2.304 ± 0.032	4.236LysGly: 4.236 ± 0.056	1.07LysHis: 1.07 ± 0.027	6.025LysIle: 6.025 ± 0.069	6.179LysLys: 6.179 ± 0.078	6.547LysLeu: 6.547 ± 0.075	2.156LysMet: 2.156 ± 0.035	4.668LysAsn: 4.668 ± 0.062	2.23LysPro: 2.23 ± 0.039	2.59LysGln: 2.59 ± 0.041	2.79LysArg: 2.79 ± 0.053	4.233LysSer: 4.233 ± 0.061	4.027LysThr: 4.027 ± 0.055	4.57LysVal: 4.57 ± 0.059	0.664LysTrp: 0.664 ± 0.024	3.266LysTyr: 3.266 ± 0.055	0.0LysXaa: 0.0 ± 0.0
Leu	5.84LeuAla: 5.84 ± 0.069	1.241LeuCys: 1.241 ± 0.029	4.948LeuAsp: 4.948 ± 0.055	5.98LeuGlu: 5.98 ± 0.071	4.156LeuPhe: 4.156 ± 0.066	5.64LeuGly: 5.64 ± 0.071	1.461LeuHis: 1.461 ± 0.036	7.677LeuIle: 7.677 ± 0.088	7.232LeuLys: 7.232 ± 0.075	8.966LeuLeu: 8.966 ± 0.112	2.409LeuMet: 2.409 ± 0.042	5.324LeuAsn: 5.324 ± 0.063	3.466LeuPro: 3.466 ± 0.057	2.681LeuGln: 2.681 ± 0.045	3.317LeuArg: 3.317 ± 0.057	6.876LeuSer: 6.876 ± 0.078	5.411LeuThr: 5.411 ± 0.064	5.163LeuVal: 5.163 ± 0.069	0.741LeuTrp: 0.741 ± 0.026	3.799LeuTyr: 3.799 ± 0.054	0.0LeuXaa: 0.0 ± 0.0
Met	1.902MetAla: 1.902 ± 0.037	0.27MetCys: 0.27 ± 0.015	1.799MetAsp: 1.799 ± 0.037	2.33MetGlu: 2.33 ± 0.041	1.027MetPhe: 1.027 ± 0.029	1.841MetGly: 1.841 ± 0.039	0.368MetHis: 0.368 ± 0.013	2.312MetIle: 2.312 ± 0.042	2.495MetLys: 2.495 ± 0.042	2.466MetLeu: 2.466 ± 0.047	0.749MetMet: 0.749 ± 0.027	1.794MetAsn: 1.794 ± 0.032	1.038MetPro: 1.038 ± 0.028	0.867MetGln: 0.867 ± 0.025	0.92MetArg: 0.92 ± 0.025	1.662MetSer: 1.662 ± 0.041	1.441MetThr: 1.441 ± 0.034	1.772MetVal: 1.772 ± 0.037	0.179MetTrp: 0.179 ± 0.011	0.833MetTyr: 0.833 ± 0.025	0.0MetXaa: 0.0 ± 0.0
Asn	3.121AsnAla: 3.121 ± 0.044	0.707AsnCys: 0.707 ± 0.025	2.66AsnAsp: 2.66 ± 0.043	3.645AsnGlu: 3.645 ± 0.053	2.144AsnPhe: 2.144 ± 0.041	3.846AsnGly: 3.846 ± 0.054	1.025AsnHis: 1.025 ± 0.026	5.021AsnIle: 5.021 ± 0.061	4.181AsnLys: 4.181 ± 0.063	5.05AsnLeu: 5.05 ± 0.068	1.532AsnMet: 1.532 ± 0.035	3.284AsnAsn: 3.284 ± 0.063	2.248AsnPro: 2.248 ± 0.033	2.041AsnGln: 2.041 ± 0.047	2.219AsnArg: 2.219 ± 0.043	3.419AsnSer: 3.419 ± 0.058	3.305AsnThr: 3.305 ± 0.059	3.006AsnVal: 3.006 ± 0.054	0.512AsnTrp: 0.512 ± 0.018	2.633AsnTyr: 2.633 ± 0.047	0.0AsnXaa: 0.0 ± 0.0
Pro	2.151ProAla: 2.151 ± 0.045	0.407ProCys: 0.407 ± 0.018	2.26ProAsp: 2.26 ± 0.043	2.633ProGlu: 2.633 ± 0.043	1.636ProPhe: 1.636 ± 0.038	2.201ProGly: 2.201 ± 0.043	0.537ProHis: 0.537 ± 0.021	2.535ProIle: 2.535 ± 0.042	1.967ProLys: 1.967 ± 0.036	2.723ProLeu: 2.723 ± 0.05	0.856ProMet: 0.856 ± 0.023	1.501ProAsn: 1.501 ± 0.036	0.745ProPro: 0.745 ± 0.026	0.869ProGln: 0.869 ± 0.027	0.874ProArg: 0.874 ± 0.029	1.824ProSer: 1.824 ± 0.04	1.683ProThr: 1.683 ± 0.046	2.763ProVal: 2.763 ± 0.045	0.368ProTrp: 0.368 ± 0.016	1.517ProTyr: 1.517 ± 0.034	0.0ProXaa: 0.0 ± 0.0
Gln	1.991GlnAla: 1.991 ± 0.041	0.306GlnCys: 0.306 ± 0.014	1.553GlnAsp: 1.553 ± 0.03	2.062GlnGlu: 2.062 ± 0.041	1.313GlnPhe: 1.313 ± 0.029	1.77GlnGly: 1.77 ± 0.039	0.402GlnHis: 0.402 ± 0.017	2.662GlnIle: 2.662 ± 0.049	2.369GlnLys: 2.369 ± 0.044	2.831GlnLeu: 2.831 ± 0.05	0.915GlnMet: 0.915 ± 0.023	1.782GlnAsn: 1.782 ± 0.038	0.825GlnPro: 0.825 ± 0.024	0.891GlnGln: 0.891 ± 0.029	1.057GlnArg: 1.057 ± 0.028	1.696GlnSer: 1.696 ± 0.037	1.622GlnThr: 1.622 ± 0.034	1.929GlnVal: 1.929 ± 0.036	0.258GlnTrp: 0.258 ± 0.014	1.431GlnTyr: 1.431 ± 0.032	0.0GlnXaa: 0.0 ± 0.0
Arg	1.96ArgAla: 1.96 ± 0.041	0.441ArgCys: 0.441 ± 0.019	1.809ArgAsp: 1.809 ± 0.041	2.834ArgGlu: 2.834 ± 0.054	1.699ArgPhe: 1.699 ± 0.034	1.983ArgGly: 1.983 ± 0.038	0.6ArgHis: 0.6 ± 0.021	3.405ArgIle: 3.405 ± 0.052	2.956ArgLys: 2.956 ± 0.054	3.432ArgLeu: 3.432 ± 0.051	1.192ArgMet: 1.192 ± 0.029	2.288ArgAsn: 2.288 ± 0.042	1.027ArgPro: 1.027 ± 0.032	1.227ArgGln: 1.227 ± 0.03	1.517ArgArg: 1.517 ± 0.04	1.872ArgSer: 1.872 ± 0.039	1.881ArgThr: 1.881 ± 0.038	2.196ArgVal: 2.196 ± 0.042	0.33ArgTrp: 0.33 ± 0.016	1.733ArgTyr: 1.733 ± 0.043	0.0ArgXaa: 0.0 ± 0.0
Ser	3.869SerAla: 3.869 ± 0.058	0.727SerCys: 0.727 ± 0.022	3.465SerAsp: 3.465 ± 0.048	4.053SerGlu: 4.053 ± 0.053	3.049SerPhe: 3.049 ± 0.05	4.874SerGly: 4.874 ± 0.061	0.978SerHis: 0.978 ± 0.027	5.472SerIle: 5.472 ± 0.069	4.371SerLys: 4.371 ± 0.053	5.768SerLeu: 5.768 ± 0.064	1.72SerMet: 1.72 ± 0.038	3.157SerAsn: 3.157 ± 0.058	1.79SerPro: 1.79 ± 0.034	1.707SerGln: 1.707 ± 0.034	2.227SerArg: 2.227 ± 0.042	3.889SerSer: 3.889 ± 0.062	3.176SerThr: 3.176 ± 0.049	4.094SerVal: 4.094 ± 0.057	0.533SerTrp: 0.533 ± 0.019	2.732SerTyr: 2.732 ± 0.048	0.0SerXaa: 0.0 ± 0.0
Thr	4.337ThrAla: 4.337 ± 0.059	0.576ThrCys: 0.576 ± 0.021	3.323ThrAsp: 3.323 ± 0.057	3.796ThrGlu: 3.796 ± 0.059	2.381ThrPhe: 2.381 ± 0.042	4.653ThrGly: 4.653 ± 0.066	0.822ThrHis: 0.822 ± 0.023	4.712ThrIle: 4.712 ± 0.065	3.634ThrLys: 3.634 ± 0.055	5.119ThrLeu: 5.119 ± 0.061	1.391ThrMet: 1.391 ± 0.031	2.656ThrAsn: 2.656 ± 0.055	2.072ThrPro: 2.072 ± 0.057	1.453ThrGln: 1.453 ± 0.036	1.736ThrArg: 1.736 ± 0.036	3.381ThrSer: 3.381 ± 0.061	2.986ThrThr: 2.986 ± 0.066	4.415ThrVal: 4.415 ± 0.072	0.533ThrTrp: 0.533 ± 0.02	2.328ThrTyr: 2.328 ± 0.04	0.0ThrXaa: 0.0 ± 0.0
Val	3.771ValAla: 3.771 ± 0.06	0.892ValCys: 0.892 ± 0.028	3.403ValAsp: 3.403 ± 0.054	3.718ValGlu: 3.718 ± 0.056	2.917ValPhe: 2.917 ± 0.047	3.734ValGly: 3.734 ± 0.056	0.901ValHis: 0.901 ± 0.026	5.902ValIle: 5.902 ± 0.072	4.791ValLys: 4.791 ± 0.058	6.177ValLeu: 6.177 ± 0.079	1.793ValMet: 1.793 ± 0.035	3.46ValAsn: 3.46 ± 0.053	2.183ValPro: 2.183 ± 0.043	1.64ValGln: 1.64 ± 0.037	2.193ValArg: 2.193 ± 0.041	4.365ValSer: 4.365 ± 0.052	4.138ValThr: 4.138 ± 0.061	3.941ValVal: 3.941 ± 0.051	0.605ValTrp: 0.605 ± 0.019	2.603ValTyr: 2.603 ± 0.041	0.0ValXaa: 0.0 ± 0.0
Trp	0.496TrpAla: 0.496 ± 0.017	0.141TrpCys: 0.141 ± 0.01	0.57TrpAsp: 0.57 ± 0.023	0.57TrpGlu: 0.57 ± 0.02	0.438TrpPhe: 0.438 ± 0.02	0.647TrpGly: 0.647 ± 0.024	0.178TrpHis: 0.178 ± 0.012	0.697TrpIle: 0.697 ± 0.023	0.622TrpLys: 0.622 ± 0.024	0.856TrpLeu: 0.856 ± 0.025	0.294TrpMet: 0.294 ± 0.016	0.67TrpAsn: 0.67 ± 0.021	0.235TrpPro: 0.235 ± 0.012	0.306TrpGln: 0.306 ± 0.014	0.327TrpArg: 0.327 ± 0.015	0.536TrpSer: 0.536 ± 0.022	0.44TrpThr: 0.44 ± 0.017	0.531TrpVal: 0.531 ± 0.018	0.132TrpTrp: 0.132 ± 0.01	0.381TrpTyr: 0.381 ± 0.017	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.483TyrAla: 2.483 ± 0.042	0.578TyrCys: 0.578 ± 0.021	2.57TyrAsp: 2.57 ± 0.05	3.021TyrGlu: 3.021 ± 0.048	2.079TyrPhe: 2.079 ± 0.044	2.976TyrGly: 2.976 ± 0.048	0.896TyrHis: 0.896 ± 0.023	3.785TyrIle: 3.785 ± 0.058	2.936TyrLys: 2.936 ± 0.05	4.163TyrLeu: 4.163 ± 0.055	1.144TyrMet: 1.144 ± 0.029	2.543TyrAsn: 2.543 ± 0.04	1.622TyrPro: 1.622 ± 0.033	1.545TyrGln: 1.545 ± 0.032	1.814TyrArg: 1.814 ± 0.036	2.794TyrSer: 2.794 ± 0.049	2.42TyrThr: 2.42 ± 0.042	2.456TyrVal: 2.456 ± 0.043	0.405TyrTrp: 0.405 ± 0.017	2.211TyrTyr: 2.211 ± 0.054	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4505 proteins (1407776 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

---