Methanothermus fervidus (strain ATCC 43054 / DSM 2088 / JCM 10308 / V24 S)

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Methanomada group; Methanobacteria; Methanobacteriales; Methanothermaceae; Methanothermus; Methanothermus fervidus

Average proteome isoelectric point is 7.65

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1283 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E3GYR0|E3GYR0_METFV 30S ribosomal protein S10 OS=Methanothermus fervidus (strain ATCC 43054 / DSM 2088 / JCM 10308 / V24 S) OX=523846 GN=rps10 PE=3 SV=1
MM1 pKa = 7.37SKK3 pKa = 8.47ITIDD7 pKa = 3.46YY8 pKa = 8.17DD9 pKa = 3.47KK10 pKa = 11.39CDD12 pKa = 3.94GPDD15 pKa = 3.28CAEE18 pKa = 4.51CVDD21 pKa = 4.05VCPMEE26 pKa = 4.1ILVIEE31 pKa = 4.38GDD33 pKa = 3.65KK34 pKa = 11.11VVAKK38 pKa = 9.3NTEE41 pKa = 3.89EE42 pKa = 4.45CNEE45 pKa = 4.33CMVCMDD51 pKa = 4.06VCPNEE56 pKa = 4.81AITVEE61 pKa = 4.21PEE63 pKa = 3.59EE64 pKa = 4.2

Molecular weight: 7.06 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E3GXE8|E3GXE8_METFV Oligosaccharyl transferase OS=Methanothermus fervidus (strain ATCC 43054 / DSM 2088 / JCM 10308 / V24 S) OX=523846 GN=Mfer_0177 PE=3 SV=1
MM1 pKa = 7.46SRR3 pKa = 11.84NKK5 pKa = 10.2HH6 pKa = 4.53LAKK9 pKa = 10.5KK10 pKa = 9.83IRR12 pKa = 11.84LSKK15 pKa = 10.87AMRR18 pKa = 11.84QNRR21 pKa = 11.84RR22 pKa = 11.84VPAWVMLKK30 pKa = 9.48TGRR33 pKa = 11.84RR34 pKa = 11.84NIRR37 pKa = 11.84NPKK40 pKa = 7.41EE41 pKa = 3.72RR42 pKa = 11.84HH43 pKa = 3.49WRR45 pKa = 11.84RR46 pKa = 11.84NKK48 pKa = 10.73LKK50 pKa = 10.65VV51 pKa = 3.09

Molecular weight: 6.35 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1283

0

1283

383747

39

1726

299.1

33.7

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.98 ± 0.062

1.306 ± 0.034

5.06 ± 0.043

7.481 ± 0.079

3.84 ± 0.053

6.704 ± 0.058

1.692 ± 0.028

10.022 ± 0.059

10.602 ± 0.1

8.854 ± 0.067

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.325 ± 0.027

4.832 ± 0.063

4.069 ± 0.038

1.624 ± 0.032

4.138 ± 0.05

5.328 ± 0.044

4.576 ± 0.068

7.154 ± 0.057

0.728 ± 0.024

3.683 ± 0.044
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski